LOCUS       pNHT7::ENO::sNluc2        3087 bp    DNA     circular UNA 28-JAN-2021
DEFINITION  Combination of Enolase 5' and 3' UTRs flanking sNluc2 .
ACCESSION   urn.local...9x-cq598q2
VERSION     urn.local...9x-cq598q2
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     source          join(1..>553,<1352..3087)
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     terminator      268..295
                     /note="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
                     /label="rrnB T2 terminator"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /label="Escherichia coli rrnB terminator"
     primer_bind     537..553
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="M13 fwd primer bind"
     misc_feature    <550..553
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        550..553
                     /label="Ligation"
     source          join(complement(<1346..>1355),complement(<550..>572))
                     /organism="Cloning vector pUC19"
                     /mol_type="other DNA"
                     /label="source Cloning vector pUC19"
     source          <550..>553
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     promoter        554..572
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     source          complement(<554..>572)
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     misc_feature    569..572
                     /created_by="richardhall"
                     /label="T1"
     ligation        569..572
                     /label="Ligation"
     5'UTR           573..662
                     /created_by="nhall"
                     /label="ENO 5' UTR"
     misc_feature    663..1179
                     /Source="referenceToDocument: urn:local:.:b0-ccuatug"
                     /label="Digestion of smedNLuc2 Insert - Fragment 2
                     (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    663..>666
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        663..666
                     /label="Ligation"
     CDS             663..1178
                     /created_by="nhall"
                     /label="sNluc2"
     misc_feature    1176..666
                     /Source="referenceToDocument: urn:local:.:a3-ccuasap"
                     /label="Digestion of pNHT7::Enolase extraction - Fragment
                     2 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    <1176..1179
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        1176..1179
                     /label="Ligation"
     misc_feature    1179
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     3'UTR           1179..1345
                     /created_by="nhall"
                     /label="ENO 3' UTR"
     misc_feature    1346..1351
                     /created_by="richardhall"
                     /label="HindIII"
     misc_feature    <1346..>1355
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        1346..1349
                     /label="Ligation"
     source          complement(<1346..>1351)
                     /organism="Cloning vector pUC19"
                     /mol_type="other DNA"
                     /label="source Cloning vector pUC19"
     primer_bind     complement(1352..1368)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="M13 rev primer bind"
     ligation        1352..1355
                     /label="Ligation"
     source          <1352..>1355
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     CDS             1481..2290
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin in bacteria or G418
                      (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                     PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
                     GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
                     /label="aph(3')-Ia CDS"
     rep_origin      2437..3025
                     /direction="RIGHT"
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori rep origin"
ORIGIN      
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agttaatacg actcactata gggttttagt aagtattata aatagtaaac
      601 catataaatt gcgtacgtga ttacagaaat tatatttgta atttacaaat tttttcaaga
      661 aaatggtgtt tactttggaa gattttgttg gagattggag acaaactgct ggttacaatc
      721 tggatcaggt actggaacaa ggcggtgtta gttcattatt ccaaaacctg ggtgtgagtg
      781 taactccgat tcagcgaata gtgttgtctg gagaaaatgg gctgaagatt gatatacacg
      841 tcataattcc atacgaaggc ttaagcggtg atcaaatggg acaaattgaa aaaattttta
      901 aagtagttta cccagttgac gaccatcatt ttaaagttat ccttcattac ggtacactgg
      961 ttatagatgg tgtaactcca aatatgatcg attatttcgg aagaccttac gaaggcatag
     1021 ccgtttttga tggaaaaaag attacagtaa caggtacatt gtggaacgga aataagatta
     1081 ttgacgaacg tttaattaac ccagatggaa gtttgctctt tagagttaca attaatggtg
     1141 tgacaggatg gagattatgc gaacggatac tcgcgtaatt tatatatcac tggttttgac
     1201 tctagtattt agtttggcct atattagtgt tgactgacct tgatatactt tttaccggtt
     1261 ttgtgttaat aaatattttt ctccaataat catggattca ttttgatgat tatagctata
     1321 gaaatatata aattttttgg ttgttaagct tgtcatagct gtttcctggc agctctggcc
     1381 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     1441 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     1501 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     1561 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     1621 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     1681 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     1741 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     1801 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     1861 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     1921 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     1981 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     2041 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     2101 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     2161 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     2221 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     2281 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     2341 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     2401 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2461 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2521 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2581 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2641 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2701 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2761 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2821 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2881 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2941 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3001 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3061 cttttgctgg ccttttgctc acatgtt
//
