LOCUS       Exported                3272 bp DNA     circular SYN 24-MAY-2022
DEFINITION  Standard E. coli vector with a multiple cloning site (MCS) for DNA 
            cloning. The MCS is reversed in pUC18.
ACCESSION   .
VERSION     .
KEYWORDS    pUC19
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Yanisch-Perron C, Vieira J, Messing J.
  TITLE     Improved M13 phage cloning vectors and host strains: nucleotide 
            sequences of the M13mp18 and pUC19 vectors.
  JOURNAL   Gene 1985;33:103-19.
  PUBMED    2985470
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2022 from SnapGene 6.0.4
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1985"; volume: "33"; pages: "103-19"
COMMENT     See also GenBank accession L09137.
FEATURES             Location/Qualifiers
     source          1..3272
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          397..1004
                     /chromosome="1"
                     /map="1p36.1"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9606"
                     /organism="Homo sapiens"
     source          799..816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    372..396
                     /label=Gibson
     misc_feature    397..798
                     /label=5' upstream
     misc_feature    complement(795..798)
                     /label=BbsI cut
     misc_feature    complement(801..806)
                     /label=BbsI
     misc_feature    809..814
                     /label=BbsI
     ncRNA           817..969
                     /gene="RNU1"
                     /label=U1 snRNA
                     /note="U1 small nuclear RNA; G00-119-560"
                     /ncRNA_class="snRNA"
     misc_feature    817..820
                     /label=BbsI cut
     misc_feature    970..1004
                     /label=3' downstream
     misc_feature    1005..1028
                     /label=Gibson
     protein_bind    1075..1091
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1099..1129)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(1453..2041)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2212..3072)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3073..3177)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgtaac acaggctaag gaccagcttc
      421 tttgggagag aacagacgca ggggcgggag ggaaaaaggg agaggcagac gtcacttccc
      481 cttggcggct ctggcagcag attggtcggt tgagtggcag aaaggcagac ggggactggg
      541 caaggcactg tcggtgacat cacggacagg gcgacttcta tgtagatgag gcagcgcaga
      601 ggctgctgct tcgccacttg ctgcttcacc acgaaggagt tcccgtgccc tgggagcggg
      661 ttcaggaccg ctgatcggaa gtgagaatcc cagctgtgtg tcagggctgg aaagggctcg
      721 ggagtgcgcg gggcaagtga ccgtgtgtgt aaagagtgag gcgtatgagg ctgtgtcggg
      781 gcagaggccc aagatctcgg gtcttcgaga agacctgcag gggagatacc atgatcacga
      841 aggtggtttt cccagggcga ggcttatcca ttgcactccg gatgtgctga cccctgcgat
      901 ttccccaaat gtgggaaact cgactgcata atttgtggta gtgggggact gcgttcgcgc
      961 tttcccctga ctttctggag tttcaaaagt agactgtacg ctaaatcctc tagagtcgac
     1021 ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     1081 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     1141 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     1201 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     1261 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1321 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1381 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1441 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1501 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1561 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1621 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1681 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1741 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     1801 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     1861 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1921 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1981 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2041 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2101 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2161 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     2221 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2281 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2341 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2401 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     2461 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     2521 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     2581 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     2641 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     2701 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     2761 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     2821 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     2881 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     2941 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3001 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3061 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3121 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     3181 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     3241 taaaaatagg cgtatcacga ggccctttcg tc
//
