LOCUS       Exported                5175 bp ds-DNA     circular SYN 22-JAN-2018
DEFINITION  Standard cloning vector (phagemid excised from lambda ZAP). The f1 
            (-) orientation allows rescue of antisense strand ssDNA.
ACCESSION   X52324
VERSION     .
KEYWORDS    pBluescript SK(-)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Short JM, Fernandez JM, Sorge JA, Huse WD.
  TITLE     Lambda ZAP: a bacteriophage lambda expression vector with in vivo 
            excision properties
  JOURNAL   Nucleic Acids Res. 16 (15), 7583-7600 (1988)
  PUBMED    2970625
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Stratagene
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 27, 2022 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1988"; volume: "16"; issue: "15"; pages: 
            "7583-7600"
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     rep_origin      7..462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(701..725)
                     /label=vector's primer Reverse
     misc_feature    726..1559
                     /label=left HA
     misc_feature    1560..2129
                     /label=Linker and SNAP tag
     CDS             1584..2129
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="optimized for bacterial expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     misc_feature    2130..2942
                     /label=Right HA
     misc_feature    2943..2967
                     /label=vector's primer Forward
     promoter        complement(2990..3008)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3029..3045)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3053..3069
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3077..3107)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(3431..4019)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4190..5050)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5051..5155)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
      721 atccacaaag gcgggtagag aacatatttt ccagagtggt ttgtggatat ctagggtcat
      781 gagatttcaa attcaagatt tctttgcctc aaattcttta agcgactcga tacaaggatg
      841 gaaacataaa tttaagaaag ctcgggtgtg ccagactaaa acatgagtgt agcagtctgt
      901 actagcagga gactgtgtgc aggagttaac caatcgggtg tgcaggagtt aaccaatcac
      961 cacttgaatt cctcagccaa tagtactact gcgcgggaca cttgaaaagc agacacgcct
     1021 atcaggatgc ttctcggtgg gaaggagggg tacgagcgcg ggtacgtgtg ttgcgcgtgt
     1081 gcgaacgcaa gcgtacttaa aggccaaagt gcgctactta ggtatctcac ttttccctac
     1141 ggttacttgc catggctcgt actaagcaga ccgctcgcaa gtctaccggc ggcaaggccc
     1201 cgcgcaagca gctggccacc aaggccgccc gcaagagcgc cccggccacc ggcggcgtga
     1261 agaagcctca ccgctaccgt cccggcactg tggcgctgcg cgagatccgg cgctaccaga
     1321 agtcgaccga gctgctgatc cgcaagctgc cgttccagcg cctggtgcgc gagatcgcgc
     1381 aggacttcaa gaccgacctg cgcttccaga gctcggccgt catggctctg caggaggcct
     1441 gtgaggccta cctcgtgggt ctgtttgagg acaccaacct gtgcgccatc cacgccaagc
     1501 gtgtcaccat catgcccaag gacatccagc tggcccgtcg catccgcggg gagagggctg
     1561 gaggaggatc cggaggtggc agcatggaca aagattgcga aatgaaacgt accaccctgg
     1621 atagcccgct gggcaaactg gaactgagcg gctgcgaaca gggcctgcat gaaattaaac
     1681 tgctgggtaa aggcaccagc gcggccgatg cggttgaagt tccggccccg gccgccgtgc
     1741 tgggtggtcc ggaaccgctg atgcaggcga ccgcgtggct gaacgcgtat tttcatcagc
     1801 cggaagcgat tgaagaattt ccggttccgg cgctgcatca tccggtgttt cagcaggaga
     1861 gctttacccg tcaggtgctg tggaaactgc tgaaagtggt taaatttggc gaagtgatta
     1921 gctatcagca gctggcggcc ctggcgggta atccggcggc caccgccgcc gttaaaaccg
     1981 cgctgagcgg taacccggtg ccgattctga ttccgtgcca tcgtgtggtt agctctagcg
     2041 gtgcggttgg cggttatgaa ggtggtctgg cggtgaaaga gtggctgctg gcccatgaag
     2101 gtcatcgtct gggtaaaccg ggtctgggat aagggtttct gttaatccac acaaccactt
     2161 taaaggctct tcttagagcc acccatcttc caaaaaaaga actgtagcgc tttttccaac
     2221 ttgtgggtat taatcagttt catttgtgaa agtgctaggt ctcctttcat cacaccgatc
     2281 tattataagc aagcaatatt ttggtaagtg atatctttac cctttttcat ggaaattgta
     2341 ctcttagtta gcaaagcata aagattggaa aactttgcat ggttacagta atttgggcat
     2401 ttaccgaatt taaaaataaa attattagtt gcagatgttt actttggaat tgtgaacttt
     2461 ctgaaggaca gaaccactaa tattgtaact cagaaaaatt aactgatctt ccagtcttga
     2521 aagggttaca gggatttacc attcacttta tagcgtgctt agtctcctcc ctacccacta
     2581 atagacctcc cccaccctct ttttaagtgg actcatagct taggctggtc tgcaactttc
     2641 tttgtagatc aggatgatct gtatttggga gatctgtttc tccttcctac tgtatgctgg
     2701 gattaaggtg tatatgtctt gccctccttt gggctttttg agatcctgaa tctttatggc
     2761 tctttcgtca cttgtttcac ttggcttaat aatcatcacc tcgaaaatta cagccatttg
     2821 ggcactggat ttgtcctagg tcattatcac agtggtgtat ggtgtggaaa tatgaaggct
     2881 ggtctttagg agatgtgcta gtgactataa tggaagacag ttcgaactct ggatgctgaa
     2941 aactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag
     3001 ggttaatttc gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     3061 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     3121 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     3181 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     3241 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     3301 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3361 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3421 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3481 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3541 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3601 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3661 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3721 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3781 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3841 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     3901 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3961 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4021 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     4081 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     4141 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     4201 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     4261 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4321 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4381 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4441 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4501 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     4561 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     4621 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4681 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4741 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4801 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4861 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4921 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4981 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     5041 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     5101 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     5161 ttccccgaaa agtgc
//
