LOCUS       pRRL-pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40       10135 bp    DNA     circular     30-MAY-2022
DEFINITION  .
ACCESSION   pVec3
VERSION     pVec3.1
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..232
                     /label="RSV"
     LTR             233..414
                     /label="RU5"
     gene            233..413
                     /label="HIV-1_5_LTR; truncHIV-1_3_LTR"
     misc_feature    415..432
                     /label="PBS"
     gene            524..568
                     /label="HIV-1_psi_pack"
     misc_feature    569..933
                     /label="dGag"
     misc_feature    1079..1320
                     /label="RRE"
     misc_feature    1804..1926
                     /label="PPT"
     source          1939..6589
                     /label="synthetic DNA construct"
     promoter        1939..3093
                     /modified_by="nlclarinet8"
                     /label="EF-1alpha Promoter"
     promoter        3117..3135
                     /label="T3 promoter"
     misc_feature    3190..3195
                     /label="Kozak"
     CDS             3196..3573
                     /label="H2B"
     CDS             3580..4287
                     /label="mCherry"
     CDS             4291..4344
                     /label="T2A"
     CDS             4345..4962
                     /label="rTetR"
     CDS             4963..4986
                     /label="Linker"
     CDS             4987..6147
                     /label="DNMT3L"
     promoter        complement(6175..6193)
                     /label="T7 promoter"
     polyA_signal    6206..6589
                     /created_by="nlclarinet8"
                     /label="SV40 pA"
     LTR             6661..6894
                     /label="SIN"
     gene            6661..6713
                     /label="delta_U3"
     gene            6714..6894
                     /label="HIV-1_5_LTR; truncHIV-1_3_LTR"
     misc_feature    7095..7301
                     /label="SV40 pA/Ori"
     misc_feature    7106..7183
                     /label="SV40 origin"
     promoter        complement(7274..7292)
                     /label="T7 prom"
     misc_feature    7462..7920
                     /label="F1 ORI"
     promoter        7982..8010
                     /label="amp prom"
     CDS             8052..8912
                     /label="Amp"
     promoter        9995..10024
                     /label="lac prom"
     promoter        10094..10113
                     /label="T3 prom"
ORIGIN      
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacaataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     1921 ttttatcgat cctctagaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     1981 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     2041 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     2101 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
     2161 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
     2221 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt
     2281 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
     2341 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
     2401 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
     2461 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc
     2521 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
     2581 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
     2641 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
     2701 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg
     2761 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca
     2821 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     2881 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     2941 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     3001 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     3061 caaagttttt ttcttccatt tcaggtgtcg tgagctagcg attacgccaa gctcgaaatt
     3121 aaccctcact aaagggaaca aaagctggag ctccaccgcg gtggcggccg ctctagcccg
     3181 ggcggatccg ccaccatgcc agagccagcg aagtctgctc ccgccccgaa aaagggctcc
     3241 aagaaggcgg tgactaaggc gcagaagaaa ggcggcaaga agcgcaagcg cagccgcaag
     3301 gagagctatt ccatctatgt gtacaaggtt ctgaagcagg tccaccctga caccggcatt
     3361 tcgtccaagg ccatgggcat catgaattcg tttgtgaacg acattttcga gcgcatcgca
     3421 ggtgaggctt cccgcctggc gcattacaac aagcgctcga ccatcacctc cagggagatc
     3481 cagacggccg tgcgcctgct gctgcctggg gagttggcca agcacgccgt gtccgagggt
     3541 actaaggcca tcaccaagta caccagcgct aagcctagga tggtgagcaa gggcgaggag
     3601 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     3661 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     3721 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     3781 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat tcccgactac
     3841 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     3901 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     3961 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     4021 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     4081 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     4141 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     4201 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     4261 accggcggca tggacgagct gtacaagtct gagggcaggg ggagcctcct aacttgcgga
     4321 gacgtcgagg agaatcctgg cccatctaga ctggacaaga gcaaagtcat aaacggagct
     4381 ctggaattac tcaatggtgt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag
     4441 ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat
     4501 gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag
     4561 tcatggcaag actttctgcg gaacaacgcc aagtcatacc gctgtgctct cctctcacat
     4621 cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg
     4681 gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct
     4741 ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta
     4801 gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca
     4861 attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc
     4921 atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggggtagcgg cagcggtagc
     4981 gcatgcatgg cggccatccc agccctggac ccagaggccg agcccagcat ggacgtgatt
     5041 ttggtgggat ccagtgagct ctcaagctcc gtttcacccg ggacaggcag agatcttatt
     5101 gcatatgaag tcaaggctaa ccagcgaaat atagaagaca tctgcatctg ctgcggaagt
     5161 ctccaggttc acacacagca ccctctgttt gagggaggga tctgcgcccc atgtaaggac
     5221 aagttcctgg atgccctctt cctgtacgac gatgacgggt accaatccta ctgctccatc
     5281 tgctgctccg gagagacgct gctcatctgc ggaaaccctg attgcacccg atgctactgc
     5341 ttcgagtgtg tggatagcct ggtcggcccc gggacctcgg ggaaggtgca cgccatgagc
     5401 aactgggtgt gctacctgtg cctgccgtcc tcccgaagcg ggctgctgca gcgtcggagg
     5461 aagtggcgca gccagctcaa ggccttctac gaccgagagt cggagaatcc ccttgagatg
     5521 ttcgaaaccg tgcctgtgtg gaggagacag ccagtccggg tgctgtccct ttttgaagac
     5581 atcaagaaag agctgacgag tttgggcttt ttggaaagtg gttctgaccc gggacaactg
     5641 aagcatgtgg ttgatgtcac agacacagtg aggaaggatg tggaggagtg gggacccttc
     5701 gatcttgtgt acggcgccac acctcccctg ggccacacct gtgaccgtcc tcccagctgg
     5761 tacctgttcc agttccaccg gctcctgcag tacgcacggc ccaagccagg cagccccagg
     5821 cccttcttct ggatgttcgt ggacaatctg gtgctgaaca aggaagacct ggacgtcgca
     5881 tctcgcttcc tggagatgga gccagtcacc atcccagatg tccacggcgg atccttgcag
     5941 aatgctgtcc gcgtgtggag caacatccca gccataagga gcaggcactg ggctctggtt
     6001 tcggaagaag aattgtccct gctggcccag aacaagcaga gctcgaagct cgcggccaag
     6061 tggcccacca agctggtgaa gaactgcttt ctccccctaa gagaatattt caagtatttt
     6121 tcaacagaac tcacttcctc tttataaggt accaggtaag tgtacccaat tcgccctata
     6181 gtgagtcgta ttacaattca ctcgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     6241 aatggagatc caatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
     6301 attttagatt cacagtccca aggctcattt caggcccctc agtcctcaca gtctgttcat
     6361 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     6421 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     6481 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6541 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gtacctttaa
     6601 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     6661 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     6721 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6781 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6841 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     6901 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     6961 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     7021 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7081 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc
     7141 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     7201 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcgtcgagac
     7261 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     7321 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     7381 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     7441 agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     7501 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     7561 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     7621 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     7681 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     7741 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     7801 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     7861 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttcc
     7921 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7981 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     8041 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     8101 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     8161 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     8221 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     8281 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     8341 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     8401 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     8461 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     8521 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     8581 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     8641 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8701 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8761 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8821 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8881 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8941 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     9001 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     9061 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     9121 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     9181 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     9241 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9301 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9361 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9421 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9481 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9541 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9601 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9661 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9721 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     9781 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     9841 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     9901 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     9961 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    10021 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
    10081 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagc
//
