LOCUS       pdf0112cmv-cluciferase- 6957 bp ds-DNA     circular     08-DEC-2021
DEFINITION  .
KEYWORDS    "accession:M77789" "marker:NGS R, P1-R, Gibson R"                  
FEATURES             Location/Qualifiers
     misc_feature    48..277
                     /label="Lac-operon"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    251..280
                     /label="HindIII GH"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(281..329)
                     /label="polyA signal"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             complement(330..332)
                     /label="Translation 332-330"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    333..360
                     /label="CTTTGCATTCATCTGGTACTTCTAGGGTG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     gene            complement(333..1991)
                     /label="CLuc"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(333..1991)
                     /label="Translation 1991-333"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    complement(426..432)
                     /label="Eco81I"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(1012..1042)
                     /label="NGS R"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(1051..1053)
                     /label="motif to edit"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(1054..1056)
                     /label="motif to edit"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(1076..1097)
                     /label="P3-F"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(1086..1091)
                     /label="randomize these"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(1089..1094)
                     /label="randomize these"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(1099..1118)
                     /label="NGS F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1362..1391
                     /label="Guide opt 5"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(1387..1393)
                     /label="Perfect overlap"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(1387..1404)
                     /label="Limited overlap"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(1413..1440)
                     /label="Limited overlap"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1425..1454
                     /label="Guide opt 1"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(1441..1465)
                     /label="R primer 2"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(1446..1492)
                     /label="Limited overlap"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1458..1479
                     /label="P1-R"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1498..1527
                     /label="Guide opt 2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(1522..1558)
                     /label="Limited overlap"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(1534..1555)
                     /label="P2-F"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1600..1629
                     /label="Guide opt 3"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(1609..1637)
                     /label="Limited overlap"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(1659..1673)
                     /label="Limited overlap"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1669..1698
                     /label="Guide opt 4"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(1740..1759)
                     /label="F primer 2"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(1866..1894)
                     /label="guide 6"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(1869..1898)
                     /label="APOBEC stop 11"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(1872..1900)
                     /label="guide 5"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(1873..1902)
                     /label="APOBEC stop 10"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(1877..1906)
                     /label="APOBEC stop 9"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(1878..1906)
                     /label="guide 4"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(1881..1910)
                     /label="APOBEC stop 8"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(1885..1913)
                     /label="guide 3"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(1885..1914)
                     /label="APOBEC stop 7"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(1889..1918)
                     /label="APOBEC stop 6"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(1892..1920)
                     /label="guide 2"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(1893..1922)
                     /label="APOBEC stop 5"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1897..1926)
                     /label="APOBEC stop 4"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(1900..1928)
                     /label="guide 1"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(1901..1930)
                     /label="APOBEC stop 3"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(1905..1934)
                     /label="APOBEC stop 2"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(1931..1959)
                     /label="guide seed"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(1935..1937)
                     /label="APOBEC site for making stop codon"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(1938..1967)
                     /label="APOBEC stop 1"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(1992..1997)
                     /label="Kozak"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1998..2003)
                     /label="KpnI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(2004..2009)
                     /label="HindIII"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(2016..2034)
                     /label="T7"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2026..2034)
                     /label="F primer 1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(2035..2035)
                     /label="T7"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2035..2058)
                     /label="F primer 1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        complement(2035..2689)
                     /label="PCMV"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2673..2733
                     /label="PD1712 binding"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2697..2726
                     /label="GH"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        2697..3954
                     /label="EF-1a"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3938..3967
                     /label="XbaI GH"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3969..3993
                     /label="PD4581"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3973..3978
                     /label="Kozak"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             3979..4533
                     /label="Translation 3979-4533"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    3979..4533
                     /label="G-luciferase"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(3981..4008)
                     /label="C2c2-1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(3981..4009)
                     /label="Cas13b-1"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3991..4009
                     /label="PD2757"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    4065..4084
                     /label="FP1"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(4073..4092)
                     /label="PD2758"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(4119..4138)
                     /label="Anneal"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(4131..4151)
                     /label="Anneal"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(4135..4164)
                     /label="6-guide-2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(4139..4174)
                     /label="Semi degenerate oligo (b)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(4142..4171)
                     /label="Canonical-2"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(4142..4171)
                     /label="g1"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(4142..4171)
                     /label="15 guide 2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(4142..4171)
                     /label="Canonical guide 2"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(4152..4185)
                     /label="Semi degenerate oligo (a/c)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(4155..4182)
                     /label="C2c2-1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(4156..4186)
                     /label="guide 4"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(4175..4204)
                     /label="GH"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(4186..4215)
                     /label="GH"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(4274..4418)
                     /label="C-U Reporter mutagenesis site 2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(4295..4324)
                     /label="15 guide 5"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(4295..4324)
                     /label="Canonical-5"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(4296..4325)
                     /label="Canonical guide 5"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(4296..4325)
                     /label="g2"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(4312..4339)
                     /label="C2c2-2"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    4515..4533
                     /label="Gibson R"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(4518..4536)
                     /label="PD4582"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             4534..4536
                     /label="Translation 4534-4536"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    4537..4585
                     /label="Poly A Signal"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(4590..4595)
                     /label="KpnI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4607..4636
                     /label="EcoRI GH"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(4766..4865)
                     /label="pBR322"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(4866..5002)
                     /label="pBR322"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(join(5003..6957,1..47))
                     /label="pBR322"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    5098..5202
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             5203..5271
                     /label="Translation 5203-5271"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             5272..6063
                     /label="Translation 5272-6063"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
ORIGIN
        1 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
       61 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
      121 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
      181 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
      241 caatttcaca caggaaacag ctatgaccat gattacgcca CACACAAAAA ACCAACACAC
      301 AGATGTAATG AAAATAAAGA TATTTTATTT TAtttgcatt catctggtac ttctagggtg
      361 tctccatgct ttatgtagca ttctttcttg aactcccatg cgtggtcaca gaaaccctgt
      421 tgtcccctca ggcaatactc gtacatgcat cgttcgacac ggtcaggctt gtggcacacg
      481 ttacatttct gatcaagatc actttgaccg gcgaagagac tattgcagag tcgttcagct
      541 tcaggtttct gttcttcggt gcatcccggt gggttggggg tcagatcaca ggctccttca
      601 gcatcaaaag aatcatcact gaaatcctgg ttgtagttac cgcaaatacc gcaagtctta
      661 ccatcgaatg gatctctaat atgtacgacg agcagttgct tgaagttgaa cttgaccacc
      721 agagcttcag gtaggatggc tgtagtcagt atgtcaccat cttgccagta gatggaagtg
      781 ttctgagagc tgtacgggac ggacacggct tctcctccaa ccagaatctg ttttccatca
      841 acaatgagtt ctactacagt cgattgtttc ctgattcgta ctttctctac ttcagtgtaa
      901 gagtcaacat tcctgtgtga aaccttcaca tcccaagtgt tccagaaaca gtcggcggcc
      961 ataagaatct ccttgcaggg accctggaat tggtatcttg ctttgtcaaa tgtatcgtag
     1021 aaagtatgtc cagacaaaac gcaggttcct ctagtttcgg gagcagcgca cgtctccctg
     1081 tagtcaagca gcacgtgtga ggctcgctcg tctccaccca tacagcgggc gaaggcgcag
     1141 gagatggtgt agtagtagaa gttgatgggg ttgcggcagc tgtccttgta cggctccaga
     1201 agacctttgc agtatgcaac gtcatcagga tttccataga gtggacaacc gtcaaactcc
     1261 tggttgatct taggctgaat agcgagttgt tctggatctg aagtgaagtc tgtatcttcc
     1321 atcattttaa gatctccaca gaggccagag atcattcctt tgtttgctgt gtctggggcg
     1381 attcttacag atcttcctcc gaggatgtcg atcacgatca gtttgaagaa ctcaatgacg
     1441 gtgatgttga agcctggcat ctcaacaaca gcgatggtga cctcgccgat ggtgtacggg
     1501 ttggcgatga tagggtcagc tccaccgttt acagtgatgg gattctcagt agcttgagcg
     1561 atgtcaatga tgtctccagc cacttccagt cttgtcttgg tcagcacagc ccccttggtt
     1621 ccatccaggt tctccagggt gatggacacc ttccagtcgc cgcccttggt tccttgaccc
     1681 aacacgtatg taccaggttc ctggaactgg aatctctttc catagaatgt tctaaaccat
     1741 cctgcggcct ctactctgca ttcaattaca tactgacaca ttcggcaaca tgtttttcct
     1801 ggtttatttt cacacagtcc atctgatagt atgtctctcg tgcaggtgcc acagctgcta
     1861 tcaatacatt ctccttcttt agcttcacag gaagttggaa ctgtgtttgg tggatcaggt
     1921 tcgtaaggac agtcctggca atgaacagtg gcgcagtaga ctaatgcaac ggcaagaatt
     1981 aaggtcttca tggtggcggt accaagcttg ggtctcccta tagtgagtcg tattaatttc
     2041 gataagccag taagcagtgg gttctctagt tagccagaga gctctgctta tatagacctc
     2101 ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg
     2161 aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact
     2221 tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat
     2281 caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa
     2341 ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg
     2401 cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatagtc
     2461 cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat
     2521 tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta
     2581 tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg attactatta
     2641 ataactagtc aataatcaat gtcaacgcgt atatctggcc cgtacatcgA AGCTGAtgca
     2701 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
     2761 gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     2821 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
     2881 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     2941 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     3001 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
     3061 ttgcgtgcct tgaattactt ccactggctg cagtacgtga ttcttgatcc cgagcttcgg
     3121 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
     3181 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
     3241 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
     3301 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
     3361 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     3421 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     3481 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     3541 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     3601 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     3661 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     3721 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     3781 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     3841 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     3901 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgacGTACG
     3961 ggatcctcta gagccaccat gGGAGTCAAA GTTCTGTTTG CCCTGATCTG CATCGCTGTG
     4021 GCCGAGGCCA AGCCCACCGA GAACAACGAA GACTTCAACA TCGTGGCCGT GGCCAGCAAC
     4081 TTCGCGACCA CGGATCTCGA TGCTGACCGC GGGAAGTTGC CCGGCAAGAA GCTGCCGCTG
     4141 GAGGTGCTCA AAGAGATGGA AGCCAATGCC CGGAAAGCTG GCTGCACCAG GGGCTGTCTG
     4201 ATCTGCCTGT CCCACATCAA GTGCACGCCC AAGATGAAGA AGTTCATCCC AGGACGCTGC
     4261 CACACCTACG AAGGCGACAA AGAGTCCGCA cagGGCGGCA TAGGCGAGGC GATCGTCGAC
     4321 ATTCCTGAGA TTCCTGGGTT CAAGGACTTG GAGCCCATGG AGCAGTTCAT CGCACAGGTC
     4381 GATCTGTGTG TGGACTGCAC AACTGGCTGC CTCAAAGGGC TTGCCAACGT GCAGTGTTCT
     4441 GACCTGCTCA AGAAGTGGCT GCCGCAACGC TGTGCGACCT TTGCCAGCAA GATCCAGGGC
     4501 CAGGTGGACA AGATCAAGGG GGCCGGTGGT GACTAAAATA AAATATCTTT ATTTTCATTA
     4561 CATCTGTGTG TTGGTTTTTT GTGTGcccgg gtaccgagct cgaattcact ggccgtcgtt
     4621 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     4681 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     4741 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
     4801 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     4861 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     4921 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     4981 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     5041 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     5101 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5161 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5221 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5281 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5341 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     5401 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     5461 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5521 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5581 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5641 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5701 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     5761 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     5821 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5881 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     5941 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6001 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6061 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     6121 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6181 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6241 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6301 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6361 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6421 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6481 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6541 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6601 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6661 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6721 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6781 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6841 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6901 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgc
//