LOCUS       pdf0386-pcmv-hudiscas7- 7575 bp ds-DNA     circular     08-DEC-2021
DEFINITION  .
KEYWORDS    "accession:addgene_132775_255286"                                  
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     primer_bind     complement(70..89)
                     /label="pRS-marker"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     source          join(254..7575,1..253)
                     /label="source"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     enhancer        261..640
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        641..844
                     /label="CMV promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     795..815
                     /label="CMV-F"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        886..904
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     886..905
                     /label="T7"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    918..2717
                     /label="piece 1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             918..5090
                     /label="Translation 918-5090"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             918..5090
                     /label="Translation 918-5090"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    918..5090
                     /label="DsiGRAMP"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             918..5090
                     /label="Translation 918-5090"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     exon            918..5090
                     /label="Translation 918-5090"
     exon            918..5090
                     /label="Translation 918-5090"
     exon            918..5090
                     /label="Translation 918-5090"
     CDS             918..5093
                     /label="Translation 918-5093"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     exon            918..5093
                     /label="Translation 918-5093"
     misc_feature    2718..3935
                     /label="piece 2"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3948..5090
                     /label="piece 3"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             5099..5116
                     /label="6xHis"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     exon            5099..5116
                     /label="Translation 5099-5116"
     primer_bind     complement(5139..5156)
                     /label="BGH-rev"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     polyA_signal    5145..5369
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(5440..5456)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(5440..5456)
                     /label="M13 Reverse"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(5453..5475)
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    5464..5480
                     /label="lac operator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(5488..5518)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    5533..5554
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     complement(5671..5688)
                     /label="L4440"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      complement(5842..6430)
                     /label="ori"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     complement(5922..5941)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             complement(6601..7461)
                     /label="AmpR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     exon            complement(6601..7461)
                     /label="Translation 7461-6601"
     primer_bind     7224..7243
                     /label="Amp-R"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        complement(7462..7566)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc ccctagggtc
       61 gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
      121 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
      181 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
      241 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
      301 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      361 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      421 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      481 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      541 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      601 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      661 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      721 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      781 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      841 agctggttta gtgaaccgtc agatccgcta gagatccgcg gccgctaata cgactcacta
      901 tagggagagc cgccaccATG ACGACTACTA TGAAGATTTC AATTGAATTC CTCGAGCCGT
      961 TTCGGATGAC CAAATGGCAG GAAAGCACTA GGCGAAACAA GAACAACAAG GAATTTGTTC
     1021 GCGGCCAAGC TTTTGCAAGA TGGCATAGAA ATAAGAAGGA TAACACAAAA GGGCGGCCTT
     1081 ACATAACTGG GACATTGCTG CGGTCCGCAG TAATTAGAAG CGCGGAAAAC CTGCTGACAT
     1141 TGAGTGATGG GAAAATTAGC GAAAAGACAT GTTGTCCTGG GAAATTTGAT ACGGAAGATA
     1201 AAGACAGGTT GCTGCAATTG AGGCAGCGGT CCACCCTTCG TTGGACCGAT AAGAACCCCT
     1261 GTCCAGATAA CGCTGAAACT TACTGCCCGT TTTGCGAACT TCTCGGGAGA TCCGGTAACG
     1321 ATGGAAAGAA GGCTGAGAAG AAGGATTGGC GCTTTCGTAT TCATTTTGGC AATCTTTCCC
     1381 TTCCAGGGAA GCCCGATTTC GACGGGCCAA AAGCTATAGG CAGCCAACGG GTACTTAACA
     1441 GGGTCGATTT CAAATCCGGT AAGGCCCATG ACTTCTTTAA GGCTTACGAA GTCGACCATA
     1501 CTAGGTTTCC CCGCTTCGAA GGGGAGATTA CCATAGATAA TAAAGTCAGC GCTGAAGCCA
     1561 GGAAACTGCT CTGTGATTCT CTCAAGTTTA CGGATCGGCT GTGTGGAGCT CTGTGCGTAA
     1621 TCAGGTTCGA TGAATATACA CCAGCAGCCG ATAGTGGGAA ACAAACCGAA AATGTCCAAG
     1681 CAGAACCGAA TGCTAATCTC GCTGAAAAGA CCGCAGAGCA AATTATTAGC ATTCTGGACG
     1741 ATAATAAGAA AACCGAATAT ACCCGCCTGC TTGCTGATGC TATTCGTTCA CTCCGCCGCT
     1801 CTAGTAAACT TGTCGCTGGC TTGCCAAAGG ATCATGACGG GAAGGATGAC CATTACCTCT
     1861 GGGATATCGG CAAGAAGAAG AAAGACGAAA ACTCTGTCAC TATTAGGCAA ATCCTTACGA
     1921 CCTCAGCAGA CACCAAGGAA CTCAAGAATG CCGGAAAATG GAGAGAATTC TGCGAGAAGC
     1981 TGGGTGAAGC GCTGTACCTC AAGAGTAAAG ACATGAGTGG CGGCCTGAAA ATTACTCGGA
     2041 GAATACTGGG CGATGCTGAA TTCCATGGAA AGCCCGATCG GCTTGAAAAG AGCCGGTCTG
     2101 TGTCAATCGG ATCTGTGTTG AAAGAAACTG TGGTATGCGG CGAACTGGTC GCTAAAACGC
     2161 CCTTCTTCTT CGGAGCGATA GACGAAGATG CAAAACAAAC CgacCTGCAA GTACTCCTCA
     2221 CTCCCGATAA CAAGTATAGA CTGCCAAGAA GCGCCGTGCG AGGTATACTC CGTCGAGATC
     2281 TTCAAACCTA TTTTGATAGC CCATGCAATG CTGAGTTGGG TGGACGGCCA TGCATGTGTA
     2341 AGACGTGTAG GATTATGAGA GGGATCACGG TGATGGATGC GCGCAGTGAG TACAATGCCC
     2401 CGCCAGAAAT AAGGCATCGT ACCCGCATTA ATCCCTTCAC AGGCACGGTT GCGGAAGGTG
     2461 CCCTGTTTAA TATGGAAGTA GCCCCCGAGG GGATTGTCTT TCCATTTCAA CTCCGGTACC
     2521 GGGGCTCTGA AGATGGGCTG CCCGATGCAC TGAAAACGGT GTTGAAATGG TGGGCTGAGG
     2581 GGCAGGCATT CATGAGTGGC GCTGCCTCAA CCGGGAAGGG CCGATTCCGG ATGGAAAATG
     2641 CTAAATATGA AACGCTGGAT CTGAGCGACG AGAATCAAAG GAATGACTAT CTTAAGAATT
     2701 GGGGATGGCG TGACGAGAAG GGGCTCGAGG AACTGAAGAA ACGACTGAAC TCAGGTCTGC
     2761 CAGAGCCCGG TAATTATAGG GATCCAAAAT GGCACGAGAT TAACGTTTCC ATTGAGATGG
     2821 CAAGCCCTTT TATTAATGGC GACCCAATCC GCGCAGCCGT GGACAAACGT GGTACAgatG
     2881 TGGTTACCTT CGTTAAGTAT AAAGCTGAAG GGGAAGAGGC GAAACCCGTA TGTGCATACA
     2941 AGGCCGAATC TTTTAGAGGG GTGATCAGAA GTGCCGTGGC ACGCATTCAT ATGGAAGATG
     3001 GCGTCCCTTT GACTGAGTTG ACTCACAGTG ACTGTGAATG TCTCCTGTGC CAAATCTTTG
     3061 GAAGTGAGTA TGAAGCCGGC AAAATAAGGT TTGAAGATCT CGTATTCGAA AGTGACCCGG
     3121 AACCTGTGAC CTTCGATCAT GTGGCCATCG ATAGATTCAC TGGCGGTGCA GCTGATAAGA
     3181 AGAAATTCGA TGATTCCCCT CTGCCCGGTA GCCCTGCAAG ACCGCTCATG TTGAAAGGCT
     3241 CCTTCTGGAT CCGCAGGGAC GTTCTCGAGG ACGAAGAGTA CTGTAAGGCA CTCGGTAAGG
     3301 CTCTTGCAGA TGTGAATAAT GGCCTTTATC CCCTCGGTGG AAAGAGCGCC ATCGGCTACG
     3361 GACAGGTCAA GAGTCTGGGT ATAAAGGGAG ATGATAAGAG GATTTCTCGC CTCATGAATC
     3421 CTGCCTTTGA TGAGACAGAT GTAGCCGTTC CAGAAAAGCC CAAAACTGAT GCCGAGGTTC
     3481 GCATCGAGGC AGAGAAAGTA TATTACCCAC ACTATTTCGT CGAACCCCAT AAGAAGGTGG
     3541 AACGCGAGGA GAAACCCTGT GGTCATCAAA AGTTCCACGA GGGGCGACTG ACAGGTAAAA
     3601 TTCGGTGTAA GCTCATTACC AAGACACCCC TCATCGTCCC AGATACTAGT AATGACGATT
     3661 TCTTCAGACC TGCGGATAAA GAAGCTCGGA AGGAAAAGGA CGAATATCAT AAATCATATG
     3721 CTTTCTTCAG ACTTCATAAA CAAATCATGA TTCCCGGGAG CGAATTGAGA GGAATGGTGA
     3781 GTAGTGTCTA CGAAACTGTG ACAAATTCTT GCTTCAGGAT ATTTGATGAG ACTAAACGGT
     3841 TGTCATGGCG GATGGATGCT GATCACCAGA ATGTGCTGCA AGACTTTCTC CCAGGTCGAG
     3901 TGACCGCCGA TGGGAAACAT ATACAAAAGT TTTCCggggg tgggtcaGAT CTGAAGGAGA
     3961 ACGAGGAATA CGAGATCCCC GAGAAAGCAA GAATCAAATA CAAAGAACTG CTCAGGGTCT
     4021 ATAACAACAA TCCTCAAGCA GTGCCGGAGA GCGTATTTCA GTCTAGAGTT GCCCGGGAAA
     4081 ACGTGGAAAA GCTGAAGTCC GGAGATCTTG TGTATTTCAA ACATAATGAA AAGTACGTAG
     4141 AGGACATCGT CCCAGTGCGG ATTTCCCGAA CTGTAGACGA TAGGATGATC GGCAAACGTA
     4201 TGAGCGCCGA TCTGCGGCCG TGCCATGGAG ATTGGGTGGA AGATGGTGAT CTCAGTGCCT
     4261 TGAATGCATA TCCCGAGAAA AGACTCCTCT TGCGCCACCC CAAAGGACTC TGCCCTGCTT
     4321 GCCGGCTCTT TGGAACCGGA TCTTACAAGG GCAGAGTCAG GTTTGGATTC GCGTCACTCG
     4381 AAAACGATCC GGAGTGGCTG ATCCCAGGCA AGAATCCCGG CGATCCGTTT CACGGCGGGC
     4441 CGGTGATGCT CTCATTGTTG GAACGGCCTC GCCCGACTTG GAGTATACCG GGATCCGACA
     4501 ATAAGTTTAA AGTGCCTGGC AGAAAGTTTT ACGTCCACCA CCACGCCTGG AAAACCATTA
     4561 AGGACGGGAA CCATCCCACA ACAGGCAAAG CTATTGAACA AAGCCCTAAT AACCGCACTG
     4621 TAGAAGCTCT CGCCGGCGGG AATTCCTTTA GCTTCGAAAT TGCCTTTGAG AACCTGAAAG
     4681 AATGGGAGCT GGGTTTGCTC ATCCACAGCC TGCAACTCGA AAAGGGTCTG GCGCATAAAC
     4741 TTGGAATGGC AAAGTCTATG GGATTTGGTT CAGTTGAAAT TGACGTCGAA TCAGTGCGCC
     4801 TGAGAAAAGA TTGGAAGCAA TGGCGGAATG GCAATTCCGA AATTCCCAAC TGGTTGGGAA
     4861 AAGGATTTGC TAAACTGAAG GAATGGTTCC GGGACGAGCT CGATTTTATA GAAAATCTTA
     4921 AGAAACTTCT TTGGTTTCCT GAGGGCGACC AAGCACCCCG GGTTTGCTAC CCCATGCTGC
     4981 GAAAGAAGGA CGATCCTAAT GGGAATAGCG GTTACGAAGA ACTCAAAGAC GGGGAATTCA
     5041 AGAAAGAAGA TCGGCAGAAG AAGCTGACCA CGCCGTGGAC ACCGTGGGCA taaccggtca
     5101 tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     5161 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     5221 cactgtcctt tcctaataaa atgagaaaat tgcatcgcat tgtctgagta ggtgtcattc
     5281 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     5341 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     5401 gataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     5461 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     5521 ctagggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     5581 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     5641 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     5701 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     5761 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     5821 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     5881 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     5941 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6001 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6061 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6121 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6181 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6241 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     6301 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6361 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6421 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6481 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6541 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     6601 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     6661 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     6721 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     6781 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     6841 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     6901 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     6961 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7021 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7081 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7141 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7201 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7261 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7321 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7381 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7441 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7501 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7561 tccgcgcaca tttcc
//