LOCUS       pdf0582-huDisCas7-11-R1 8258 bp ds-DNA     circular     12-DEC-2021
DEFINITION  .
KEYWORDS    "marker:AmpR, Amp-R, F1ori-R, AAV2 ITR, AAV2 ITR"                  
FEATURES             Location/Qualifiers
     source          1..8258
                     /label="source"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     complement(11..28)
                     /label="L4440"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     rep_origin      complement(182..770)
                     /label="ori"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(262..281)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(941..1801)
                     /label="AmpR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     1564..1583
                     /label="Amp-R"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        complement(1802..1906)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     rep_origin      complement(1932..2387)
                     /label="f1 ori"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     complement(2069..2090)
                     /label="F1ori-F"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     2281..2300
                     /label="F1ori-R"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     repeat_region   2459..2588
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2768..2785
                     /label="AttP seq Bxb1"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     source          2768..8013
                     /label="source"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        2829..3040
                     /label="EF-1α core promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     polyA_signal    7637..7685
                     /label="poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        7686..7926
                     /label="U6 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    7935..7988
                     /label="mature DR crRNA"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     source          7951..7981
                     /label="source"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     repeat_region   8074..8203
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
     2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
     2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
     2641 gaagagtacc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     2701 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     2761 tgtatcaGTG GTTTGTCTGG TCAACtaggt cttgaaagga gtgggaattg gctccggtgc
     2821 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     2881 caattgatcc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     2941 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     3001 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gGCCACATGA CGACTACTAT
     3061 GAAGATTTCA ATTGAATTCC TCGAGCCGTT TCGGATGACC AAATGGCAGG AAAGCACTAG
     3121 GCGAAACAAG AACAACAAGG AATTTGTTCG CGGCCAAGCT TTTGCAAGAT GGCATAGAAA
     3181 TAAGAAGGAT AACACAAAAG GGCGGCCTTA CATAACTGGG ACATTGCTGC GGTCCGCAGT
     3241 AATTAGAAGC GCGGAAAACC TGCTGACATT GAGTGATGGG AAAATTAGCG AAAAGACATG
     3301 TTGTCCTGGG AAATTTGATA CGGAAGATAA AGACAGGTTG CTGCAATTGA GGCAGCGGTC
     3361 CACCCTTCGT TGGACCGATA AGAACCCCTG TCCAGATAAC GCTGAAACTT ACTGCCCGTT
     3421 TTGCGAACTT CTCGGGAGAT CCGGTAACGA TGGAAAGAAG GCTGAGAAGA AGGATTGGCG
     3481 CTTTCGTATT CATTTTGGCA ATCTTTCCCT TCCAGGGAAG CCCGATTTCG ACGGGCCAAA
     3541 AGCTATAGGC AGCCAACGGG TACTTAACAG GGTCGATTTC AAATCCGGTA AGGCCCATGA
     3601 CTTCTTTAAG GCTTACGAAG TCGACCATAC TAGGTTTCCC CGCTTCGAAG GGGAGATTAC
     3661 CATAGATAAT AAAGTCAGCG CTGAAGCCAG GAAACTGCTC TGTGATTCTC TCAAGTTTAC
     3721 GGATCGGCTG TGTGGAGCTC TGTGCGTAAT CAGGTTCGAT GAATATACAC CAGCAGCCGA
     3781 TAGTGGGAAA CAAACCGAAA ATGTCCAAGC AGAACCGAAT GCTAATCTCG CTGAAAAGAC
     3841 CGCAGAGCAA ATTATTAGCA TTCTGGACGA TAATAAGAAA ACCGAATATA CCCGCCTGCT
     3901 TGCTGATGCT ATTCGTTCAC TCCGCCGCTC TAGTAAACTT GTCGCTGGCT TGCCAAAGGA
     3961 TCATGACGGG AAGGATGACC ATTACCTCTG GGATATCGGC AAGAAGAAGA AAGACGAAAA
     4021 CTCTGTCACT ATTAGGCAAA TCCTTACGAC CTCAGCAGAC ACCAAGGAAC TCAAGAATGC
     4081 CGGAAAATGG AGAGAATTCT GCGAGAAGCT GGGTGAAGCG CTGTACCTCA AGAGTAAAGA
     4141 CATGAGTGGC GGCCTGAAAA TTACTCGGAG AATACTGGGC GATGCTGAAT TCCATGGAAA
     4201 GCCCGATCGG CTTGAAAAGA GCCGGTCTGT GTCAATCGGA TCTGTGTTGA AAGAAACTGT
     4261 GGTATGCGGC GAACTGGTCG CTAAAACGCC CTTCTTCTTC GGAGCGATAG ACGAAGATGC
     4321 AAAACAAACC gacCTGCAAG TACTCCTCAC TCCCGATAAC AAGTATAGAC TGCCAAGAAG
     4381 CGCCGTGCGA GGTATACTCC GTCGAGATCT TCAAACCTAT TTTGATAGCC CATGCAATGC
     4441 TGAGTTGGGT GGACGGCCAT GCATGTGTAA GACGTGTAGG ATTATGAGAG GGATCACGGT
     4501 GATGGATGCG CGCAGTGAGT ACAATGCCCC GCCAGAAATA AGGCATCGTA CCCGCATTAA
     4561 TCCCTTCACA GGCACGGTTG CGGAAGGTGC CCTGTTTAAT ATGGAAGTAG CCCCCGAGGG
     4621 GATTGTCTTT CCATTTCAAC TCCGGTACCG GGGCTCTGAA GATGGGCTGC CCGATGCACT
     4681 GAAAACGGTG TTGAAATGGT GGGCTGAGGG GCAGGCATTC ATGAGTGGCG CTGCCTCAAC
     4741 CGGGAAGGGC CGATTCCGGA TGGAAAATGC TAAATATGAA ACGCTGGATC TGAGCGACGA
     4801 GAATCAAAGG AATGACTATC TTAAGAATTG GGGATGGCGT GACGAGAAGG GGCTCGAGGA
     4861 ACTGAAGAAA CGACTGAACT CAGGTCTGCC AGAGCCCGGT AATTATAGGG ATCCAAAATG
     4921 GCACGAGATT AACGTTTCCA TTGAGATGGC AAGCCCTTTT ATTAATGGCG ACCCAATCCG
     4981 CGCAGCCGTG GACAAACGTG GTACAgatGT GGTTACCTTC GTTAAGTATA AAGCTGAAGG
     5041 GGAAGAGGCG AAACCCGTAT GTGCATACAA GGCCGAATCT TTTAGAGGGG TGATCAGAAG
     5101 TGCCGTGGCA CGCATTCATA TGGAAGATGG CGTCCCTTTG ACTGAGTTGA CTCACAGTGA
     5161 CTGTGAATGT CTCCTGTGCC AAATCTTTGG AAGTGAGTAT GAAGCCGGCA AAATAAGGTT
     5221 TGAAGATCTC GTATTCGAAA GTGACCCGGA ACCTGTGACC TTCGATCATG TGGCCATCGA
     5281 TAGATTCACT GGCGGTGCAG CTGATAAGAA GAAATTCGAT GATTCCCCTC TGCCCGGTAG
     5341 CCCTGCAAGA CCGCTCATGT TGAAAGGCTC CTTCTGGATC CGCAGGGACG TTCTCGAGGA
     5401 CGAAGAGTAC TGTAAGGCAC TCGGTAAGGC TCTTGCAGAT GTGAATAATG GCCTTTATCC
     5461 CCTCGGTGGA AAGAGCGCCA TCGGCTACGG ACAGGTCAAG AGTCTGGGTA TAAAGGGAGA
     5521 TGATAAGAGG ATTTCTCGCC TCATGAATCC TGCCTTTGAT GAGACAGATG TAGCCGTTCC
     5581 AGAAAAGCCC AAAACTGATG CCGAGGTTCG CATCGAGGCA GAGAAAGTAT ATTACCCACA
     5641 CTATTTCGTC GAACCCCATA AGAAGGTGGA ACGCGAGGAG AAACCCTGTG GTCATCAAAA
     5701 GTTCCACGAG GGGCGACTGA CAGGTAAAAT TCGGTGTAAG CTCATTACCA AGACACCCCT
     5761 CATCGTCCCA GATACTAGTA ATGACGATTT CTTCAGACCT GCGGATAAAG AAGCTCGGAA
     5821 GGAAAAGGAC GAATATCATA AATCATATGC TTTCTTCAGA CTTCATAAAC AAATCATGAT
     5881 TCCCGGGAGC GAATTGAGAG GAATGGTGAG TAGTGTCTAC GAAACTGTGA CAAATTCTTG
     5941 CTTCAGGATA TTTGATGAGA CTAAACGGTT GTCATGGCGG ATGGATGCTG ATCACCAGAA
     6001 TGTGCTGCAA GACTTTCTCC CAGGTCGAGT GACCGCCGAT GGGAAACATA TACAAAAGTT
     6061 TTCCGAAACT GCAAGGGTGC CTTTCTATGA CAAAACGCAG AAACATTTTG ACATTCTTGA
     6121 TGAGCAAGAA ATTGCTGGTG AGAAACCTGT TCGGATGTGG GTCAAGCGTT TTATTAAACG
     6181 Agggggtggg tcaAGAGATA GTAGATACCA AAAGGCCTTT CAAGAGATAC CCGAGAACGA
     6241 CCCAGACGGA TGGGAGTGTA AAGAGGGCTA CCTTCATGTC GTCGGCCCCA GCAAAGTAGA
     6301 GTTTAGTGAC AAGAAAGGGG ATGTGATCAA TAACTTTCAA GGAACACTCC CATCAGTTCC
     6361 CAACGACTGG AAAACAATTA GGACGAACGA TTTTAAGAAT AGGAAAAGGA AGAATGAACC
     6421 TGTGTTTTGT TGTGAGGATG ATAAGGGCAA TTACTATACC ATGGCTAAAT ATTGCGAAAC
     6481 CTTCTTCTTC GATCTGAAGG AGAACGAGGA ATACGAGATC CCCGAGAAAG CAAGAATCAA
     6541 ATACAAAGAA CTGCTCAGGG TCTATAACAA CAATCCTCAA GCAGTGCCGG AGAGCGTATT
     6601 TCAGTCTAGA GTTGCCCGGG AAAACGTGGA AAAGCTGAAG TCCGGAGATC TTGTGTATTT
     6661 CAAACATAAT GAAAAGTACG TAGAGGACAT CGTCCCAGTG CGGATTTCCC GAACTGTAGA
     6721 CGATAGGATG ATCGGCAAAC GTATGAGCGC CGATCTGCGG CCGTGCCATG GAGATTGGGT
     6781 GGAAGATGGT GATCTCAGTG CCTTGAATGC ATATCCCGAG AAAAGACTCC TCTTGCGCCA
     6841 CCCCAAAGGA CTCTGCCCTG CTTGCCGGCT CTTTGGAACC GGATCTTACA AGGGCAGAGT
     6901 CAGGTTTGGA TTCGCGTCAC TCGAAAACGA TCCGGAGTGG CTGATCCCAG GCAAGAATCC
     6961 CGGCGATCCG TTTCACGGCG GGCCGGTGAT GCTCTCATTG TTGGAACGGC CTCGCCCGAC
     7021 TTGGAGTATA CCGGGATCCG ACAATAAGTT TAAAGTGCCT GGCAGAAAGT TTTACGTCCA
     7081 CCACCACGCC TGGAAAACCA TTAAGGACGG GAACCATCCC ACAACAGGCA AAGCTATTGA
     7141 ACAAAGCCCT AATAACCGCA CTGTAGAAGC TCTCGCCGGC GGGAATTCCT TTAGCTTCGA
     7201 AATTGCCTTT GAGAACCTGA AAGAATGGGA GCTGGGTTTG CTCATCCACA GCCTGCAACT
     7261 CGAAAAGGGT CTGGCGCATA AACTTGGAAT GGCAAAGTCT ATGGGATTTG GTTCAGTTGA
     7321 AATTGACGTC GAATCAGTGC GCCTGAGAAA AGATTGGAAG CAATGGCGGA ATGGCAATTC
     7381 CGAAATTCCC AACTGGTTGG GAAAAGGATT TGCTAAACTG AAGGAATGGT TCCGGGACGA
     7441 GCTCGATTTT ATAGAAAATC TTAAGAAACT TCTTTGGTTT CCTGAGGGCG ACCAAGCACC
     7501 CCGGGTTTGC TACCCCATGC TGCGAAAGAA GGACGATCCT AATGGGAATA GCGGTTACGA
     7561 AGAACTCAAA GACGGGGAAT TCAAGAAAGA AGATCGGCAG AAGAAGCTGA CCACGCCGTG
     7621 GACACCGTGG GCAtaaaata aaatatcttt attttcatta caTCTGTGTG TTGGTTTTTT
     7681 GTGTGgaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta
     7741 gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
     7801 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
     7861 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     7921 aaggacgaaa caccgTTGat gtcacggaac GGTAATGCCT GGCTTGTCGA CGCATAGTCT
     7981 Gtttttttaa gcttgggccg ctcgaggggc ccgggatctt cctagagcat ggctacgtag
     8041 ataagtagca tggcgggtta atcattaact acaaggaacc cctagtgatg gagttggcca
     8101 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     8161 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcatt aatgaatcgg
     8221 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgct
//