LOCUS       Exported               11453 bp DNA     circular SYN 30-MAY-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11453)
  AUTHORS   Hermann
  TITLE     Direct Submission
  JOURNAL   Exported May 31, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11453
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     LTR             377..634
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     497..516
                     /label=Set 1 - Pair 10
                     /note="color: black; sequence: GCTAACTAGAGAACCCACTG"
     rep_origin      1248..1409
                     /direction=RIGHT
                     /label=rep
                     /note="rep"
                     /note="color: #c0c0c0"
     rep_origin      1261..1396
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     misc_signal     1410..1559
                     /label=PPT
                     /note="  PPT"
                     /note="color: #ff8000; direction: RIGHT"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     CDS             2092..2136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /note="color: #cc99b2"
                     /translation="KNEQELLELDKWASL"
     enhancer        2406..2709
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2725..3906
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        3928..3946
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     3955..3984
                     /label=Fragment.FOR
                     /note="color: black; sequence: 
                     AGCTTGGTACCGAGCTCGCCACCATGCCTG; added: 2020-01-29"
     CDS             3978..4655
                     /codon_start=1
                     /label=CD79A
                     /note="color: #993366"
                     /translation="MPGGPGVLQALPATIFLLFLLSAVYLGPGCQALWMHKVPASLMVS
                     LGEDAHFQCPHNSSNNANVTWWRVLHGNYTWPPEFLGPGEDPNGTLIIQNVNKSHGGIY
                     VCRVQEGNESYQQSCGTYLRVRQPPPRPFLDMGEGTKNRIITAEGIILLFCAVVPGTLL
                     LFRKRWQNEKLGLDAGDEYEDENLYEGLNLDDCSMYEDISRGLQGTYQDVGSLNIGDVQ
                     LEKP"
     misc_feature    4407..4472
                     /label=TM
                     /note="color: #000080"
     misc_structure  4539..4550
                     /label=ITAM1
                     /note="color: #0000ff"
     misc_structure  4572..4583
                     /label=ITAM2
                     /note="color: #3366ff"
     misc_signal     4605..4616
                     /label=ITAM3
                     /note="color: #3366ff"
     primer_bind     complement(4640..4675)
                     /label=Fragment.REV
                     /note="color: black; sequence: 
                     ACCATGGTGGCGACCGGGTCCGGCTTCTCCAGCTGG; added: 2020-01-29"
     regulatory      4665..4674
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             4671..5390
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 4671 .. 4673 / #e1ff2f
                        2: 4674 .. 4676 / #e1ff2f / 1a
                        3: 4677 .. 5390 / #e1ff2f"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(5418..5419)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     LTR             5650..6283
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     6146..6165
                     /label=Set 1 - Pair 10
                     /note="color: black; sequence: GCTAACTAGAGAACCCACTG"
     rep_origin      complement(8096..8684)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(8855..9715)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8855 .. 9646 / #ccffcc
                        2: 9647 .. 9715 / #ccffcc / signal sequence
                     Cleavage site after base 9646"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9716..9820)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    10065..10203
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
ORIGIN
        1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
      121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
      181 aataaggaga gaagaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
      241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
      301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa
      841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
      901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
      961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
     1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
     1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
     1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatat gactccgccc
     1261 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     1321 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     1381 ggcttttttg gaggcctagg cttttgcaaa tgcataaaag aaaagggggg attggggggt
     1441 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     1501 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1861 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatttggaa taacatgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctgt agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatctagtta actagatcta
     2401 gagtccgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     2461 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     2521 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     2581 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     2641 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     2701 attaccatgg tgagcatgcg tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc
     2761 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
     2821 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     2881 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     2941 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
     3001 atggcccttg cgtgccttga attacttcca cgcccctggc tgcagtacgt gattcttgat
     3061 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
     3121 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
     3181 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
     3241 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
     3301 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
     3361 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
     3421 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
     3481 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
     3541 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
     3601 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
     3661 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
     3721 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa
     3781 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga
     3841 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg
     3901 tcgtgaggct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagcttg
     3961 gtaccgagct cgccaccatg cctgggggtc caggagtcct ccaagctctg cctgccacca
     4021 tcttcctcct cttcctgctg tctgctgtct acctgggccc tgggtgccag gccctgtgga
     4081 tgcacaaggt cccagcatca ttgatggtga gcctggggga agacgcccac ttccaatgcc
     4141 cgcacaatag cagcaacaac gccaacgtca cctggtggcg cgtcctccat ggcaactaca
     4201 cgtggccccc tgagttcttg ggcccgggcg aggaccccaa tggtacgctg atcatccaga
     4261 atgtgaacaa gagccatggg ggcatatacg tgtgccgggt ccaggagggc aacgagtcat
     4321 accagcagtc ctgcggcacc tacctccgcg tgcgccagcc gccccccagg cccttcctgg
     4381 acatggggga gggcaccaag aaccgaatca tcacagccga ggggatcatc ctcctgttct
     4441 gcgcggtggt gcctgggacg ctgctgctgt tcaggaaacg atggcagaac gagaagctcg
     4501 ggttggatgc cggggatgaa tatgaagatg aaaaccttta tgaaggcctg aacctggacg
     4561 actgctccat gtatgaggac atctcccggg gcctccaggg cacctaccag gatgtgggca
     4621 gcctcaacat aggagatgtc cagctggaga agccggaccc ggtcgccacc atggtgagca
     4681 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     4741 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     4801 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     4861 ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     4921 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     4981 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     5041 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     5101 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     5161 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     5221 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
     5281 accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     5341 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg
     5401 actctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg
     5461 actgtgcctt ctagttgcca ggagcaatca caagtagcaa tacagcagct aacaatgctg
     5521 cttgcygcct ggctagaagc acaagaggag gaagaggtgg gttttccagt cacacctcag
     5581 gtacctttaa gaccaatgac ttacaggcag ctgtagatct tagccacttt ttaaaagaaa
     5641 aggggggact ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     5701 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccaggggtca
     5761 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     5821 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catggaatgg
     5881 atgaccctga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     5941 tggcccgaga gctgcatccg gagtacttca agaactgctg acatcgagct tgctacaagg
     6001 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     6061 ccctcagatg ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     6121 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     6181 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     6241 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcacccccca ggaggtagag
     6301 gttgcagtga gccaagatcg cgccactgca ttccagcctg ggcaagaaaa caagactgtc
     6361 taaaataata ataataagtt aagggtatta aatatattta tacatggagg tcataaaaat
     6421 atatatattt gggctgggcg cagtggctca cacctgcgcc cggccctttg ggaggccgag
     6481 gcaggtggat cacctgagtt tgggagttcc agaccagcct gaccaacatg gagaaacccc
     6541 ttctctgtgt atttttagta gattttattt tatgtgtatt ttattcacag gtatttctgg
     6601 aaaactgaaa ctgtttttcc tctactctga taccacaaga atcatcagca cagaggaaga
     6661 cttctgtgat caaatgtggt gggagaggga ggttttcacc agcacatgag cagtcagttc
     6721 tgccgcagac tcggcgggtg tccttcggtt cagttccaac accgcctgcc tggagagagg
     6781 tcagaccaca gggtgagggc tcagtcccca agacataaac acccaagaca taaacaccca
     6841 acaggtccac cccgcctgct gcccaggcag agccgattca ccaagacggg aattaggata
     6901 gagaaagagt aagtcacaca gagccggctg tgcgggagaa cggagttcta ttatgactca
     6961 aatcagtctc cccaagcatt cggggatcag agtttttaag gataacttag tgtgtagggg
     7021 gccagtgagt tggagatgaa agcgtaggga gtcgaaggtg tccttttgcg ccgagtcagt
     7081 tcctgggtgg gggccacaag atcggatgag ccagtttatc aatccggggg tgccagctga
     7141 tccatggagt gcagggtctg caaaatatct caagcactga ttgatcttag gttttacaat
     7201 agtgatgtta ccccaggaac aatttgggga aggtcagaat cttgtagcct gtagctgcat
     7261 gactcctaaa ccataatttc ttttttgttt tttttttttt atttttgaga cagggtctca
     7321 ctctgtcacc taggctggag tgcagtggtg caatcacagc tcactgcagc ctcaacgtcg
     7381 taagctcaag cgatcctccc acctcagcct gcctggtagc tgagactaca agcgacgccc
     7441 cagttaattt ttgtattttt ggtagaggca gcgttttgcc gtgtggccct ggctggtctc
     7501 gaactcctgg gctcaagtga tccagcctca gcctcccaaa gtgctgggac aaccggggcc
     7561 agtcactgca cctggcccta aaccataatt tctaatcttt tggctaattt gttagtccta
     7621 caaaggcagt ctagtcccca ggcaaaaagg gggtttgttt cgggaaaggg ctgttactgt
     7681 ctttgtttca aactataaac taagttcctc ctaaacttag ttcggcctac acccaggaat
     7741 gaacaaggag agcttggagg ttagaagcac gatggaattg gttaggtcag atctctttca
     7801 ctgtctgagt tataattttg caatggtggt tcaaagactg cccgcttctg acaccagtcg
     7861 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg cgctcttccg
     7921 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7981 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     8041 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8101 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8161 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8221 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8281 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8341 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8401 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8461 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8521 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8581 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8641 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8701 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8761 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8821 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8881 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8941 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     9001 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9061 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9121 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9181 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9241 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9301 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9361 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9421 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9481 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9541 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9601 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9661 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9721 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9781 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9841 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     9901 cgaggccctt tcgtcttcaa gaactgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     9961 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
    10021 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
    10081 tcacgtagcg atagcggagt gtactggctt aactatgcgg catcagagca gattgtactg
    10141 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
    10201 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
    10261 tcgctattac gccaggggag gcagagattg cagtaagctg agatcgcagc actgcactcc
    10321 agcctgggcg acagagtaag actctgtctc aaaaataaaa taaataaatc aatcagatat
    10381 tccaatcttt tcctttattt atttatttat tttctatttt ggaaacacag tccttcctta
    10441 ttccagaatt acacatatat tctatttttc tttatatgct ccagtttttt ttagaccttc
    10501 acctgaaatg tgtgtataca aaatctaggc cagtccagca gagcctaaag gtaaaaaata
    10561 aaataataaa aaataaataa aatctagctc actccttcac atcaaaatgg agatacagct
    10621 gttagcatta aataccaaat aacccatctt gtcctcaata attttaagcg cctctctcca
    10681 ccacatctaa ctcctgtcaa aggcatgtgc cccttccggg cgctctgctg tgctgccaac
    10741 caactggcat gtggactctg cagggtccct aactgccaag ccccacagtg tgccctgagg
    10801 ctgccccttc cttctagcgg ctgcccccac tcggctttgc tttccctagt ttcagttact
    10861 tgcgttcagc caaggtctga aactaggtgc gcacagagcg gtaagactgc gagagaaaga
    10921 gaccagcttt acagggggtt tatcacagtg caccctgaca gtcgtcagcc tcacaggggg
    10981 tttatcacat tgcaccctga cagtcgtcag cctcacaggg ggtttatcac agtgcaccct
    11041 tacaatcatt ccatttgatt cacaattttt ttagtctcta ctgtgcctaa cttgtaagtt
    11101 aaatttgatc agaggtgtgt tcccagaggg gaaaacagta tatacagggt tcagtactat
    11161 cgcatttcag gcctccacct gggtcttgga atgtgtcccc cgaggggtga tgactacctc
    11221 agttggatct ccacaggtca cagtgacaca agataaccaa gacacctccc aaggctacca
    11281 caatgggccg ccctccacgt gcacatggcc ggaggaactg ccatgtcgga ggtgcaagca
    11341 cacctgcgca tcagagtcct tggtgtggag ggagggacca gcgcagcttc cagccatcca
    11401 cctgatgaac agaacctagg gaaagcccca gttctactta caccaggaaa ggc
//
