LOCUS       Exported               11465 bp DNA     circular SYN 30-MAY-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11465)
  AUTHORS   Hermann
  TITLE     Direct Submission
  JOURNAL   Exported May 31, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11465
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     LTR             377..634
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     497..516
                     /label=Set 1 - Pair 10
                     /note="color: black; sequence: GCTAACTAGAGAACCCACTG"
     rep_origin      1248..1409
                     /direction=RIGHT
                     /label=rep
                     /note="rep"
                     /note="color: #c0c0c0"
     rep_origin      1261..1396
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     misc_signal     1410..1559
                     /label=PPT
                     /note="  PPT"
                     /note="color: #ff8000; direction: RIGHT"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     CDS             2092..2136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /note="color: #cc99b2"
                     /translation="KNEQELLELDKWASL"
     enhancer        2406..2709
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2725..3906
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        3928..3946
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     3955..3984
                     /label=Fragment.FOR
                     /note="color: black; sequence: 
                     AGCTTGGTACCGAGCTCGCCACCATGGCCA; added: 2020-01-29"
     regulatory      3972..3981
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             3978..4667
                     /codon_start=1
                     /label=CD79B
                     /note="color: #993366"
                     /translation="MARLALSPVPSHWMVALLLLLSAAEPVPAARSEDRYRNPKGSACS
                     RIWQSPRFIARKRGFTVKMHCYMNSASGNVSWLWKQEMDENPQQLKLEKGRMEESQNES
                     LATLTIQGIRFEDNGIYFCQQKCNNTSEVYQGCGTELRVMGFSTLAQLKQRNTLKDGII
                     MIQTLLIILFIIVPIFLLLDKDDSKAGMEEDHTYEGLDIDQTATYEDIVTLRTGEVKWS
                     VGEHPGQE"
     misc_structure  4476..4526
                     /label=TM
                     /note="color: #ccffff"
     misc_signal     4566..4577
                     /label=ITAM1
                     /note="color: #000080"
     misc_structure  4599..4610
                     /label=ITAM2
                     /note="color: #3366ff"
     primer_bind     complement(4652..4687)
                     /label=Fragment.REV
                     /note="color: black; sequence: 
                     ACCATGGTGGCGACCGGGTCCTCCTGGCCTGGGTGC; added: 2020-01-29"
     regulatory      4677..4686
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             4683..5402
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 4683 .. 4685 / #e1ff2f
                        2: 4686 .. 4688 / #e1ff2f / 1a
                        3: 4689 .. 5402 / #e1ff2f"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(5430..5431)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     LTR             5662..6295
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     6158..6177
                     /label=Set 1 - Pair 10
                     /note="color: black; sequence: GCTAACTAGAGAACCCACTG"
     rep_origin      complement(8108..8696)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(8867..9727)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8867 .. 9658 / #ccffcc
                        2: 9659 .. 9727 / #ccffcc / signal sequence
                     Cleavage site after base 9658"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9728..9832)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    10077..10215
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
ORIGIN
        1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
      121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
      181 aataaggaga gaagaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
      241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
      301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa
      841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
      901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
      961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
     1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
     1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
     1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatat gactccgccc
     1261 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     1321 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     1381 ggcttttttg gaggcctagg cttttgcaaa tgcataaaag aaaagggggg attggggggt
     1441 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     1501 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1861 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatttggaa taacatgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctgt agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatctagtta actagatcta
     2401 gagtccgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     2461 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     2521 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     2581 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     2641 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     2701 attaccatgg tgagcatgcg tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc
     2761 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
     2821 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     2881 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     2941 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
     3001 atggcccttg cgtgccttga attacttcca cgcccctggc tgcagtacgt gattcttgat
     3061 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
     3121 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
     3181 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
     3241 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
     3301 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
     3361 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
     3421 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
     3481 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
     3541 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
     3601 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
     3661 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
     3721 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa
     3781 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga
     3841 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg
     3901 tcgtgaggct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagcttg
     3961 gtaccgagct cgccaccatg gccaggctgg cgttgtctcc tgtgcccagc cactggatgg
     4021 tggcgttgct gctgctgctc tcagcagctg agccagtacc agcagccaga tcggaggacc
     4081 ggtaccggaa tcccaaaggt agtgcttgtt cgcggatctg gcagagccca cgtttcatag
     4141 ccaggaaacg gggcttcacg gtgaaaatgc actgctacat gaacagcgcc tccggcaatg
     4201 tgagctggct ctggaagcag gagatggacg agaatcccca gcagctgaag ctggaaaagg
     4261 gccgcatgga agagtcccag aacgaatctc tcgccaccct caccatccaa ggcatccggt
     4321 ttgaggacaa tggcatctac ttctgtcagc agaagtgcaa caacacctcg gaggtctacc
     4381 agggctgcgg cacagagctg cgagtcatgg gattcagcac cttggcacag ctgaagcaga
     4441 ggaacacgct gaaggatggt atcatcatga tccagacgct gctgatcatc ctcttcatca
     4501 tcgtgcctat cttcctgctg ctggacaagg atgacagcaa ggctggcatg gaggaagatc
     4561 acacctacga gggcctggac attgaccaga cagccaccta tgaggacata gtgacgctgc
     4621 ggacagggga agtgaagtgg tctgtaggtg agcacccagg ccaggaggac ccggtcgcca
     4681 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     4741 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     4801 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     4861 ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
     4921 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     4981 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     5041 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     5101 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     5161 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     5221 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     5281 actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     5341 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     5401 aaagcggccg cgactctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc
     5461 tgatcagcct cgactgtgcc ttctagttgc caggagcaat cacaagtagc aatacagcag
     5521 ctaacaatgc tgcttgcygc ctggctagaa gcacaagagg aggaagaggt gggttttcca
     5581 gtcacacctc aggtaccttt aagaccaatg acttacaggc agctgtagat cttagccact
     5641 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaaagaaga caagatatcc
     5701 ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag
     5761 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     5821 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     5881 tgcatggaat ggatgaccct gagagagaag tgttagagtg gaggtttgac agccgcctag
     5941 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgacatcgag
     6001 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     6061 gggagtggcg agccctcaga tgctgcatat aagcagctgc tttttgcctg tactgggtct
     6121 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     6181 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     6241 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcaccccc
     6301 caggaggtag aggttgcagt gagccaagat cgcgccactg cattccagcc tgggcaagaa
     6361 aacaagactg tctaaaataa taataataag ttaagggtat taaatatatt tatacatgga
     6421 ggtcataaaa atatatatat ttgggctggg cgcagtggct cacacctgcg cccggccctt
     6481 tgggaggccg aggcaggtgg atcacctgag tttgggagtt ccagaccagc ctgaccaaca
     6541 tggagaaacc ccttctctgt gtatttttag tagattttat tttatgtgta ttttattcac
     6601 aggtatttct ggaaaactga aactgttttt cctctactct gataccacaa gaatcatcag
     6661 cacagaggaa gacttctgtg atcaaatgtg gtgggagagg gaggttttca ccagcacatg
     6721 agcagtcagt tctgccgcag actcggcggg tgtccttcgg ttcagttcca acaccgcctg
     6781 cctggagaga ggtcagacca cagggtgagg gctcagtccc caagacataa acacccaaga
     6841 cataaacacc caacaggtcc accccgcctg ctgcccaggc agagccgatt caccaagacg
     6901 ggaattagga tagagaaaga gtaagtcaca cagagccggc tgtgcgggag aacggagttc
     6961 tattatgact caaatcagtc tccccaagca ttcggggatc agagttttta aggataactt
     7021 agtgtgtagg gggccagtga gttggagatg aaagcgtagg gagtcgaagg tgtccttttg
     7081 cgccgagtca gttcctgggt gggggccaca agatcggatg agccagttta tcaatccggg
     7141 ggtgccagct gatccatgga gtgcagggtc tgcaaaatat ctcaagcact gattgatctt
     7201 aggttttaca atagtgatgt taccccagga acaatttggg gaaggtcaga atcttgtagc
     7261 ctgtagctgc atgactccta aaccataatt tcttttttgt tttttttttt ttatttttga
     7321 gacagggtct cactctgtca cctaggctgg agtgcagtgg tgcaatcaca gctcactgca
     7381 gcctcaacgt cgtaagctca agcgatcctc ccacctcagc ctgcctggta gctgagacta
     7441 caagcgacgc cccagttaat ttttgtattt ttggtagagg cagcgttttg ccgtgtggcc
     7501 ctggctggtc tcgaactcct gggctcaagt gatccagcct cagcctccca aagtgctggg
     7561 acaaccgggg ccagtcactg cacctggccc taaaccataa tttctaatct tttggctaat
     7621 ttgttagtcc tacaaaggca gtctagtccc caggcaaaaa gggggtttgt ttcgggaaag
     7681 ggctgttact gtctttgttt caaactataa actaagttcc tcctaaactt agttcggcct
     7741 acacccagga atgaacaagg agagcttgga ggttagaagc acgatggaat tggttaggtc
     7801 agatctcttt cactgtctga gttataattt tgcaatggtg gttcaaagac tgcccgcttc
     7861 tgacaccagt cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7921 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7981 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8041 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8101 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8161 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8221 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8281 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     8341 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8401 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8461 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8521 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     8581 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8641 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8701 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8761 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8821 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8881 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8941 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9001 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9061 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9121 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9181 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9241 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9301 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9361 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9421 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9481 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9541 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9601 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9661 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9721 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9781 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9841 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     9901 ataggcgtat cacgaggccc tttcgtcttc aagaactgcc tcgcgcgttt cggtgatgac
     9961 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
    10021 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
    10081 gccatgaccc agtcacgtag cgatagcgga gtgtactggc ttaactatgc ggcatcagag
    10141 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
    10201 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
    10261 gtgcgggcct cttcgctatt acgccagggg aggcagagat tgcagtaagc tgagatcgca
    10321 gcactgcact ccagcctggg cgacagagta agactctgtc tcaaaaataa aataaataaa
    10381 tcaatcagat attccaatct tttcctttat ttatttattt attttctatt ttggaaacac
    10441 agtccttcct tattccagaa ttacacatat attctatttt tctttatatg ctccagtttt
    10501 ttttagacct tcacctgaaa tgtgtgtata caaaatctag gccagtccag cagagcctaa
    10561 aggtaaaaaa taaaataata aaaaataaat aaaatctagc tcactccttc acatcaaaat
    10621 ggagatacag ctgttagcat taaataccaa ataacccatc ttgtcctcaa taattttaag
    10681 cgcctctctc caccacatct aactcctgtc aaaggcatgt gccccttccg ggcgctctgc
    10741 tgtgctgcca accaactggc atgtggactc tgcagggtcc ctaactgcca agccccacag
    10801 tgtgccctga ggctgcccct tccttctagc ggctgccccc actcggcttt gctttcccta
    10861 gtttcagtta cttgcgttca gccaaggtct gaaactaggt gcgcacagag cggtaagact
    10921 gcgagagaaa gagaccagct ttacaggggg tttatcacag tgcaccctga cagtcgtcag
    10981 cctcacaggg ggtttatcac attgcaccct gacagtcgtc agcctcacag ggggtttatc
    11041 acagtgcacc cttacaatca ttccatttga ttcacaattt ttttagtctc tactgtgcct
    11101 aacttgtaag ttaaatttga tcagaggtgt gttcccagag gggaaaacag tatatacagg
    11161 gttcagtact atcgcatttc aggcctccac ctgggtcttg gaatgtgtcc cccgaggggt
    11221 gatgactacc tcagttggat ctccacaggt cacagtgaca caagataacc aagacacctc
    11281 ccaaggctac cacaatgggc cgccctccac gtgcacatgg ccggaggaac tgccatgtcg
    11341 gaggtgcaag cacacctgcg catcagagtc cttggtgtgg agggagggac cagcgcagct
    11401 tccagccatc cacctgatga acagaaccta gggaaagccc cagttctact tacaccagga
    11461 aaggc
//
