LOCUS       Exported               10086 bp ds-DNA     circular SYN 22-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   VB190204-1030beg
VERSION     .
KEYWORDS    pLV-Hygro-CMV-hOGG1_EGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported to SnapGene on Mar 04, 2019
REFERENCE   2  (bases 1 to 10086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 22, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /label=RSV promoter
                     /note="/application_notes=Strong promoter; drives 
                     transcription of viral RNA in packaging cells."
                     /note="/description=Rous sarcoma virus enhancer/promoter"
                     /note="/full_name=Rous sarcoma virus (RSV) 
                     enhancer/promoter"
                     /note="/official_designation=RSV"
                     /note="/uuid=3f2ddca7f0cb4015b6abcec8a25c834e"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=-Delta-5' LTR
                     /note="/application_notes=Allows transcription of viral RNA
                     and its packaging into virus."
                     /note="/description=Truncated HIV-1 5' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 5' LTR"
                     /note="/official_designation=Delta5' LTR"
                     /note="/uuid=44156a3083604321be44066133e8ecb9"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    521..565
                     /label=-Psi
                     /label=nonstandard type: Miscellaneous
                     /note="/application_notes=Allows packaging of viral RNA 
                     into virus."
                     /note="/description=HIV-1 packaging signal"
                     /note="/full_name=HIV-1 psi packaging signal"
                     /note="/official_designation=Psi"
                     /note="/uuid=284899413ff943a99f8210a11ad8c54e"
     misc_feature    1075..1308
                     /label=RRE
                     /label=nonstandard type: Miscellaneous
                     /note="/application_notes=Rev protein binding site that 
                     allows Rev-dependent nuclear export of viral RNA during 
                     viral packaging."
                     /note="/description=HIV-1 Rev response element"
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
     misc_feature    1803..1920
                     /label=cPPT
                     /label=nonstandard type: Miscellaneous
                     /note="/application_notes=Facilitates the nuclear import of
                     HIV-1 cDNA through a central DNA flap."
                     /note="/description=Central polypurine tract"
                     /note="/full_name=Central polypurine tract"
                     /note="/official_designation=cPPT"
                     /note="/uuid=f7cb29985f9f41a7a5924d3462266f42"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1959..2547
                     /label=CMV
                     /note="/application_notes=Strong promoter; may have 
                     variable strength in some cell types."
                     /note="/description=Human cytomegalovirus immediate early 
                     enhancer/promoter"
                     /note="/full_name=Human cytomegalovirus immediate early 
                     promoter"
                     /note="/official_designation=CMV"
                     /note="/uuid=bbb1016d51c4484eaa5a960df4edf21a"
     enhancer        2019..2322
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2323..2526
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    2547..2571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2572..2577
                     /label=Kozak
                     /label=nonstandard type: Miscellaneous
                     /note="/application_notes=Facilitates translation 
                     initiation of ATG start codon downstream of the Kozak 
                     sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
     CDS             2578..3612
                     /label=hOGG1[NM_002542.5](ns)
                     /note="/official_designation=NM_002542.5(ns)"
                     /note="/uuid=cdd8113ecc63429db80aeb036dfa1024"
     misc_feature    3612..3613
                     /label=No linker
                     /label=nonstandard type: Linker
                     /note="/application_notes="
                     /note="/official_designation=No marker(ns)"
                     /note="/uuid=72e1edd2c1a540b28efbb477521f8696"
     CDS             3613..4332
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    3613..4332
                     /label=EGFP
                     /label=nonstandard type: ORF
                     /note="/application_notes=Commonly used green fluorescent 
                     protein; ranked high in brightness, photostability and pH 
                     stability among all fluorescent proteins."
                     /note="/description=Enhanced green fluorescent protein; 
                     codon optimized based on a variant of wild type GFP from 
                     the jellyfish Aequorea victoria"
                     /note="/full_name=EGFP reporter gene"
                     /note="/official_designation=EGFP"
                     /note="/uuid=0470849bb38e4f4b8a1fe5d436422af3"
     protein_bind    complement(4333..4357)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4371..4968
                     /label=WPRE
                     /label=nonstandard type: Miscellaneous
                     /note="/application_notes=Enhances virus stability in 
                     packaging cells, leading to higher titer of packaged virus;
                     enhances higher expression of transgenes."
                     /note="/description=Woodchuck hepatitis virus 
                     posttranscriptional regulatory element"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptional regulatory element"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
     misc_feature    4375..4963
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(4846..4857)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4987..5497
                     /label=mPGK promoter
                     /note="/application_notes=Medium-strength promoter."
                     /note="/description=Mouse phosphoglycerate kinase 1 
                     promoter"
                     /note="/full_name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/official_designation=mPGK promoter"
                     /note="/uuid=bce93795b5fd4173b8b9abc832edad80"
     promoter        4994..5493
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5510..6535
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    5510..6535
                     /label=Hygro
                     /label=nonstandard type: Marker
                     /note="/application_notes=Allows cells to be resistant to 
                     hygromycin B."
                     /note="/description=Hygromycin resistance gene"
                     /note="/full_name=Hygromycin resistance gene"
                     /note="/official_designation=Hygro"
                     /note="/uuid=9135dd0aa64645f3aa3c381325794022"
     LTR             6606..6840
                     /label=-Delta-U3/3' LTR
                     /note="/application_notes=Allows packaging of viral RNA 
                     into virus; self-inactivates the 5' LTR by a copying 
                     mechanism during viral genome integration; contains 
                     polyadenylation signal for transcription termination."
                     /note="/description=Truncated HIV-1 3' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 3' LTR"
                     /note="/official_designation=DeltaU3/3' LTR"
                     /note="/uuid=c958755414c34372b909e20e81ebcef6"
     LTR             6607..6840
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6912..7033
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    6913..7047
                     /label=SV40 early pA
                     /note="/application_notes=Allows transcription termination 
                     and polyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Simian virus 40 early polyadenylation 
                     signal"
                     /note="/full_name=SV40 early polyadenation signal"
                     /note="/official_designation=SV40 early pA"
                     /note="/uuid=2fcb13a62e704d8095b188d9625a6a94"
     rep_origin      7073..7208
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7229..7247)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7257..7273)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      7415..7870
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7896..8000
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8001..8861
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    8001..8861
                     /label=Ampicillin
                     /label=nonstandard type: ORF
                     /note="/application_notes=Allows E. coli to be resistant to
                     ampicillin."
                     /note="/description=Ampicillin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
     rep_origin      9032..9620
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/application_notes=Facilitates plasmid replication 
                     in E. coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=64a8cb3def9f49deb6752795c2d893a8"
     protein_bind    9908..9929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9944..9974
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9982..9998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10006..10022
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10043..10061
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgta gttattaata gtaatcaatt
     1981 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     2041 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2101 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2161 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2221 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2281 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2341 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2401 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2461 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2521 agagctggtt tagtgaaccg tcagatccaa gtttgtacaa aaaagcaggc tgccaccatg
     2581 cctgcccgcg cgcttctgcc caggcgcatg gggcatcgta ctctagcctc cactcctgcc
     2641 ctgtgggcct ccatcccgtg ccctcgctct gagctgcgcc tggacctggt tctgccttct
     2701 ggacaatctt tccggtggag ggagcaaagt cctgcacact ggagtggtgt actagcggat
     2761 caagtatgga cactgactca gactgaggag cagctccact gcactgtgta ccgaggagac
     2821 aagagccagg ctagcaggcc cacaccagac gagctggagg ccgtgcgcaa gtacttccag
     2881 ctagatgtta ccctggctca actgtatcac cactggggtt ccgtggactc ccacttccaa
     2941 gaggtggctc agaaattcca aggtgtgcga ctgctgcgac aagaccccat cgaatgcctt
     3001 ttctctttta tctgttcctc caacaacaac atcgcccgca tcactggcat ggtggagcgg
     3061 ctgtgccagg cttttggacc tcggctcatc cagcttgatg atgtcaccta ccatggcttc
     3121 cccagcctgc aggccctggc tgggccagag gtggaggctc atctcaggaa gctgggcctg
     3181 ggctatcgtg cccgttacgt gagtgccagt gcccgagcca tcctggaaga acagggcggg
     3241 ctagcctggc tgcagcagct acgagagtcc tcatatgagg aggcccacaa ggccctctgc
     3301 atcctgcctg gagtgggcac caaggtggct gactgcatct gcctgatggc cctagacaag
     3361 ccccaggctg tgcccgtgga tgtccatatg tggcacattg cccaacgtga ctacagctgg
     3421 caccctacca cgtcccaggc gaagggaccg agcccccaga ccaacaagga actgggaaac
     3481 tttttccgga gcctgtgggg accttatgct ggctgggccc aagcggtgct gttcagtgcc
     3541 gacctgcgcc aatcccgcca tgctcaggag ccaccagcaa agcgcagaaa gggttccaaa
     3601 gggccggaag gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     3721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     3781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     3841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     3901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     3961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     4021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     4081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     4141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     4201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     4261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     4321 ctgtacaagt aaacccagct ttcttgtaca aagtggtgat aatcgaattc cgataatcaa
     4381 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     4441 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     4501 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     4561 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     4621 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4681 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4741 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     4801 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4861 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4921 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcggga attcccgcgg
     4981 ttcgaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     5041 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
     5101 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
     5161 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
     5221 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     5281 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     5341 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
     5401 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
     5461 ctgttctcct cttcctcatc tccgggcctt tcgacctcac gtggccacca tgaaaaagcc
     5521 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
     5581 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
     5641 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
     5701 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaatttag
     5761 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
     5821 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc
     5881 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
     5941 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
     6001 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
     6061 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
     6121 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
     6181 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
     6241 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc
     6301 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
     6361 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
     6421 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
     6481 actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagggtac
     6541 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     6601 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     6661 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6721 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6781 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6841 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     6901 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6961 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7021 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     7081 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7141 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7201 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     7261 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     7321 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     7381 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     7441 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     7501 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     7561 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     7621 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     7681 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     7741 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     7801 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     7861 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7921 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7981 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     8041 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     8101 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     8161 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     8221 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     8281 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     8341 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     8401 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     8461 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     8521 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     8581 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     8641 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     8701 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     8761 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     8821 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8881 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8941 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     9001 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     9061 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     9121 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     9181 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9241 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9301 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     9361 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     9421 agctatgaga aagcgccacg cttcccgaag agagaaaggc ggacaggtat ccggtaagcg
     9481 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     9541 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     9601 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     9661 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     9721 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     9781 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     9841 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     9901 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     9961 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
    10021 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
    10081 aagctt
//
