LOCUS       Exported                6321 bp ds-DNA     circular SYN 02-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    PAAV-Ef1a-DIO-EGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 2, 2022 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /label=L-ITR
     promoter        156..1419
                     /label=EF-1a
     misc_feature    1450..1483
                     /label=lox2722
     misc_feature    1534..1567
                     /label=loxP
     misc_feature    1570..1593
                     /label=Nu25 bot
                     /note="Nu25 bot"
     misc_feature    complement(1576..2295)
                     /label=EGFP
                     /note="EGFP"
     misc_feature    complement(2282..2300)
                     /label=Nu 25 top
                     /note="Nu 25 top"
     misc_feature    complement(2306..2339)
                     /label=lox2722
     misc_feature    complement(2390..2423)
                     /label=loxP
     misc_feature    2438..3046
                     /label=WPRE
     polyA_signal    3066..3544
                     /label=hGH polyA
     misc_feature    3584..3724
                     /label=R-ITR
     rep_origin      3816..4122
                     /direction=RIGHT
                     /label=f1 Origin
     misc_feature    4641..5498
                     /label=AmpR
     rep_origin      5649..6316
                     /direction=RIGHT
                     /label=pUC ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gtaccggatc ctctagagtc
     1441 gactccggaa taacttcgta taggatactt tatacgaagt tatgcagaat ggtagctgga
     1501 ttgtagctgc tattagcaat atgaaacctc ttaataactt cgtatagcat acattatacg
     1561 aagttatggc gcgccttact tgtacagctc gtccatgccg agagtgatcc cggcggcggt
     1621 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga
     1681 ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt
     1741 ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa
     1801 gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta
     1861 gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag
     1921 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt
     1981 gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt
     2041 gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt
     2101 cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt
     2161 cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg
     2221 gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc
     2281 gcccttgctc accatggtgg ctagcataac ttcgtataaa gtatcctata cgaagttatt
     2341 tgccttaacc cagaaattat cactgttatt ctttagaatg gtgcaaagaa taacttcgta
     2401 taatgtatgc tatacgaagt tatgaattcg atatcaagct tatcgataat caacctctgg
     2461 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     2521 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     2581 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     2641 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     2701 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     2761 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     2821 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca
     2881 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     2941 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     3001 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga
     3061 gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc
     3121 cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta
     3181 ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg
     3241 gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca
     3301 atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc
     3361 cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta
     3421 gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta
     3481 cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt
     3541 ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg
     3601 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     3661 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     3721 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     3781 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3841 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     3901 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     3961 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     4021 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4081 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4141 gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     4201 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     4261 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     4321 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     4381 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     4441 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     4501 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4561 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4621 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4681 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4741 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4801 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4861 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     4921 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     4981 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5041 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5101 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5161 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5221 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5281 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5341 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5401 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5461 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5521 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5581 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5641 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5701 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5761 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     5821 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5881 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5941 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6001 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6061 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6121 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6181 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6241 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6301 gctggccttt tgctcacatg t
//
