LOCUS       Exported                7447 bp DNA     circular SYN 01-JUN-2022
DEFINITION  DDIT4_∆8.
ACCESSION   urn.local.mhunkeler.3o-evugnea
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 7, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1359..1665
                     /label=f1 origin
                     /label=nonstandard type: origin of replication
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=f1 origin"
                     /note="/Transferred_Similarity=100.00%"
     promoter        2114..2142
                     /label=amp prom
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=amp prom"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    2184..3044
                     /label=amp marker
                     /label=nonstandard type: marker
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=amp marker"
                     /note="/Transferred_Similarity=99.88%"
     misc_feature    3199..3818
                     /label=pBR322 origin
                     /label=nonstandard type: origin of replication
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=pBR322 origin"
                     /note="/Transferred_Similarity=99.68%"
     gene            4040..5186
                     /label=Ac_5_flank
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=Ac_5_flank"
                     /note="/Transferred_Similarity=100.00%"
     gene            5197..5230
                     /label=loxP
                     /label=loxP; loxH
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=loxP, loxH"
                     /note="/Transferred_Similarity=100.00% -> 91.18%"
     gene            complement(5197..5230)
                     /label=loxP
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=loxP"
                     /note="/Transferred_Similarity=82.35%"
     terminator      complement(5255..5525)
                     /label=TK_PA term
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=TK_PA term"
                     /note="/Transferred_Similarity=98.89%"
     misc_feature    complement(5546..6223)
                     /direction=LEFT
                     /label=DsRed2 reporter
                     /label=nonstandard type: reporter
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=DsRed2 reporter"
                     /note="/Transferred_Similarity=86.87%"
     promoter        6386..6486
                     /label=PH prom
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=PH prom"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    6499..6528
                     /label=StrepII-Tag
                     /note="/created_by=User"
     misc_feature    6541..6561
                     /label=TEV site
                     /note="/created_by=User"
     misc_feature    6565..7253
                     /label=pMH324_pNTM_Flag_Ddit4_∆Nshort (digested by KpnI,
                     NotI) Fragment 1 (concatenated sequence 2)
                     /label=nonstandard type: Concatenated sequence
                     /note="/Source=referenceToDocument:
                     urn:local:mhunkeler:16x-bntdbw0"
     misc_feature    6565..7253
                     /label=gBlock Ddit4 ∆Nshort (concatenated sequence 2)
                     /label=nonstandard type: Concatenated sequence
                     /note="/Source=referenceToDocument:
                     urn:local:mhunkeler:1a6-bkv0e4o"
     misc_feature    6565..6573
                     /label=HsCD00327445 Ddit4 PCR product (concatenated
                     sequence 1)
                     /label=nonstandard type: Concatenated sequence
                     /note="/Source=referenceToDocument:
                     urn:local:mhunkeler:ia-9reamth"
     CDS             6574..7248
                     /codon_start=1
                     /label=DDIT ∆1-8
                     /note="/created_by=mhunkeler"
                     /note="/modified_by=mhunkeler"
                     /translation="SSSSTSSSPSSLPRTPTPDRPPRSAWGSATREEGFDRSTSLESSD
                     CESLDSSNSGFGPEEDTAYLDGVSLPDFELLSDPEDEHLCANLMQLLQESLAQARLGSR
                     RPARLLMPSQLVSQVGKELLRLAYSEPCGLRGALLDVCVEQGKSCHSVGQLALDPSLVP
                     TFQLTLVLRLDSRLWPKIQGLFSSANSPFLPGFSQSLTLSTGFRVIKKKLYSSEQLLIE
                     EC"
     misc_feature    7246..7248
                     /label=HsCD00327445 Ddit4 PCR product (concatenated
                     sequence 1)
                     /label=nonstandard type: Concatenated sequence
                     /note="/Source=referenceToDocument:
                     urn:local:mhunkeler:40-evugneq"
     misc_feature    7249..7253
                     /label=nonstandard type: Editing History Insertion
                     /note="/Original_Bases"
     misc_feature    7249..7253
                     /label=HsCD00327445 Ddit4 PCR product (concatenated
                     sequence 1)
                     /label=nonstandard type: Concatenated sequence
                     /note="/Source=referenceToDocument:
                     urn:local:mhunkeler:ia-9reamth"
     misc_feature    join(7250..7447,1..6568)
                     /label=pMH016_pAC8RedNK_Strep (digested by KpnI, NotI)
                     Fragment 2 (concatenated sequence 1)
                     /label=nonstandard type: Concatenated sequence
                     /note="/Source=referenceToDocument:
                     urn:local:mhunkeler:17g-bntdhae"
     misc_feature    7250..7253
                     /label=Digestion of pMH064_pNTM_Strep - Fragment 2
                     (concatenated sequence 2)
                     /label=nonstandard type: Concatenated sequence
                     /note="/Source=referenceToDocument:
                     urn:local:mhunkeler:jj-9reb2zu"
     misc_feature    7250..7253
                     /label=pMH065_pNTM_Flag (digested by KpnI, NotI) Fragment
                     2 (concatenated sequence 1)
                     /label=nonstandard type: Concatenated sequence
                     /note="/Source=referenceToDocument:
                     urn:local:mhunkeler:442-blpi91d"
     gene            join(7285..7447,1..1271)
                     /label=Ac_3_flank
                     /note="/Imported_from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla 
                     sMapper</a>"
                     /note="/Transferred_From=Ac_3_flank"
                     /note="/Transferred_Similarity=100.00%"
ORIGIN
        1 atcctcaata gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga
       61 accgatggct ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg
      121 tagcagcaat ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac
      181 gtcgttcaaa atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg
      241 actcgacgta aacacgttaa ataaagcttg gacatattta acatcgggcg tgttagcttt
      301 attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga
      361 ttttgccata gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt
      421 tgtagttgag ctttttggaa ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct
      481 aactgtgccc gattttaatt cagacaacac gttagaaagc gatggtgcag gcggtggtaa
      541 catttcagac ggcaaatcta ctaatggcgg cggtggtgga gctgatgata aatctaccat
      601 cggtggaggc gcaggcgggg ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc
      661 agacggcggt ttaggctcaa atgtctcttt aggcaacaca gtcggcacct caactattgt
      721 actggtttcg ggcgccgttt ttggtttgac cggtctgaga cgagtgcgat ttttttcgtt
      781 tctaatagct tccaacaatt gttgtctgtc gtctaaaggt gcagcgggtt gaggttccgt
      841 cggcattggt ggagcgggcg gcaattcaga catcgatggt ggtggtggtg gtggaggcgc
      901 tggaatgtta ggcacgggag aaggtggtgg cggcggtgcc gccggtataa tttgttctgg
      961 tttagtttgt tcgcgcacga ttgtgggcac cggcgcaggc gccgctggct gcacaacgga
     1021 aggtcgtctg cttcgaggca gcgcttgggg tggtggcaat tcaatattat aattggaata
     1081 caaatcgtaa aaatctgcta taagcattgt aatttcgcta tcgtttaccg tgccgatatt
     1141 taacaaccgc tcaatgtaag caattgtatt gtaaagagat tgtctcaagc tcggatcgat
     1201 cccgcacgcc gataacaagc cttttcattt ttactacagc attgtagtgg cgagacactt
     1261 cgctgtcgtc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     1321 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     1381 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     1441 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     1501 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
     1561 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     1621 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     1681 cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     1741 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
     1801 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     1861 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     1921 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     1981 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     2041 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     2101 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2161 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     2221 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     2281 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     2341 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     2401 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     2461 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     2521 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     2581 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     2641 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     2701 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     2761 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     2821 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     2881 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     2941 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     3001 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     3061 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     3121 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3181 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3241 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3301 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3361 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3421 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3481 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3541 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3601 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3661 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3721 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3781 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3841 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3901 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3961 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     4021 ggccgattca ttaatgcaga acggctccgc ccactattaa tgaaattaaa aattccaatt
     4081 ttaaaaaacg cagcaagaga aacatttgta tgaaagaatg cgtagaagga aagaaaaatg
     4141 tcgtcgacat gctgaacaac aagattaata tgcctccgtg tataaaaaaa atattgaacg
     4201 atttgaaaga aaacaatgta ccgcgcggcg gtatgtacag gaagaggttt atactaaact
     4261 gttacattgc aaacgtggtt tcgtgtgcca agtgtgaaaa ccgatgttta atcaaggctc
     4321 tgacgcattt ctacaaccac gactccaagt gtgtgggtga agtcatgcat cttttaatca
     4381 aatcccaaga tgtgtataaa ccaccaaact gccaaaaaat gaaaactgtc gacaagctct
     4441 gtccgtttgc tggcaactgc aagggtctca atcctatttg taattattga ataataaaac
     4501 aattataaat gctaaatttg ttttttatta acgatacaaa ccaaacgcaa caagaacatt
     4561 tgtagtatta tctataattg aaaacgcgta gttataatcg ctgaggtaat atttaaaatc
     4621 attttcaaat gattcacagt taatttgcga caatataatt ttattttcac ataaactaga
     4681 cgccttgtcg tcttcttctt cgtattcctt ctctttttca tttttctcct cataaaaatt
     4741 aacatagtta ttatcgtatc catatatgta tctatcgtat agagtaaatt ttttgttgtc
     4801 ataaatatat atgtcttttt taatggggtg tatagtaccg ctgcgcatag tttttctgta
     4861 atttacaaca gtgctatttt ctggtagttc ttcggagtgt gttgctttaa ttattaaatt
     4921 tatataatca atgaatttgg gatcgtcggt tttgtacaat atgttgccgg catagtacgc
     4981 agcttcttct agttcaatta caccattttt tagcagcacc ggattaacat aactttccaa
     5041 aatgttgtac gaaccgttaa acaaaaacag ttcacctccc ttttctatac tattgtctgc
     5101 gagcagttgt ttgttgttaa aaataacagc cattgtaatg agacgcacaa actaatatca
     5161 caaactggaa atgtctatca atatatagtt gctgatataa cttcgtatag catacattat
     5221 acgaagttat ctggttgaaa cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     5281 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     5341 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     5401 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     5461 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     5521 cccccatctc ccgctattac tcgagtcact gggagccgga gtggcgggcc tcggcgtgct
     5581 cgtactgctc caccacggtg tagtcctcgt tgtggttggt gatgtccagc ttggagtcca
     5641 cgtagtggtt gccgggcagc tgcacgggct tcttggcctt gtacacggtc ttgaagtcgc
     5701 aggtgtagtg gccgccgtcc ttcagcttca gggcgtggga gatctcgccc ttcagcacgc
     5761 cgtcctgggg gtacagcttc tcggtggagg gctcccagcc ggcagtcttc ttctgcatta
     5821 cggggccgtc ggcggggaag ttcacgccct tgaacttcac cttgtagatg aaggtgccgt
     5881 cctgcaggga ggagtcctgc tgcacctcca ccacgccgcc gtcctcgaag ttcatggagc
     5941 gctcccaggt gaagccctcg gggaaggaca gcttcatgta gtcggggatg tcggcggggt
     6001 gcttcacgta ggccttggag ccgtactgga actgggggga caggatgtcc caggcgaagg
     6061 gcagggggcc gcccttggtc acctgcagct tggcggtctg ggtgccctcg tagggcttgc
     6121 cctcgccctc gccctcgatc tcgaagtagt ggccgttcac ggagccctcc atgcgcacct
     6181 tgaactgcat gaactccttg atgacgtcct cggtgttgtc catggtagag tgattgtaaa
     6241 taaaatgtaa tttacagtat agtattttaa ttaatataca aatgatttga taataattct
     6301 tatttaacta taatatattg tgttgggttg aattaaaggt ccgtatacta gtatcgattc
     6361 gcgacctact ccggaatatt aatagagttg ctgatatcat ggagataatt aaaatgataa
     6421 ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc
     6481 tataaatacg gatctaccat ggactggagc cacccgcagt tcgaaaaatc cgcggtcgac
     6541 gagaacctct acttccaggg aggcggccgc accagttctt cctccacgag cagttctcct
     6601 tcatctctcc cgagaacgcc aacgcccgac agacctccga gatctgcgtg gggatcagca
     6661 actagagagg agggatttga tcgctcaacc tcacttgaaa gttctgactg cgagagcctt
     6721 gacagcagta attcaggttt tggaccagag gaagataccg cgtatttgga cggcgtcagc
     6781 cttccggact ttgagctgct cagcgatcct gaagacgagc atctctgtgc taatctcatg
     6841 cagttgcttc aggagagtct tgcccaggcc cgccttggta gtcgaaggcc ggcgaggttg
     6901 ttgatgccgt cccaattggt ctcccaagtc ggcaaagaac ttcttcggct ggcgtattcc
     6961 gagccctgcg ggttgcgcgg cgctctgctt gatgtctgcg ttgaacaggg gaaatcatgt
     7021 cattctgttg ggcaacttgc cctggaccct tcccttgtgc cgacattcca actcacgctt
     7081 gttctgaggt tggacagtcg gctgtggcca aaaattcagg gtcttttttc ttccgcaaat
     7141 tctcccttct tgccaggctt ctcccagtct ttgacacttt caaccggatt ccgagtcata
     7201 aagaagaagc tctatagttc tgagcagttg cttatcgaag agtgctgagg tacccccgga
     7261 cggccgctta attaattgat ccgggttatt agtacattta ttaagcgcta gattctgtgc
     7321 gttgttgatt tacagacaat tgttgtacgt attttaataa ttcattaaat ttataatctt
     7381 tagggtggta tgttagagcg aaaatcaaat gattttcagc gtctttatat ctgaatttaa
     7441 atattaa
//
