LOCUS       Exported               17870 bp DNA     circular SYN 01-JUN-2022
DEFINITION  Isoform 1, ∆3291-3298, Expression clone.
ACCESSION   urn.local.mhunkeler.5r-evul7mx
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 7, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..17870
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     143..159
                     /label=M13 fwd
                     /note="<html><body>common sequencing primer, one of
                     multiple similar variants</body></html>"
     enhancer        312..690
                     /label=CMV enhancer
                     /note="<html><body>human cytomegalovirus immediate early 
                     enhancer</body></html>"
     promoter        691..894
                     /label=CMV promoter
                     /note="<html><body>human cytomegalovirus (CMV) immediate
                     early promoter</body></html>"
     CDS             928..951
                     /codon_start=1
                     /product="<html><body>FLAG(R) epitope tag, followed by an 
                     enterokinase cleavage site</body></html>"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    989..1009
                     /label=attB1
                     /label=nonstandard type: attB1
                     /note="/annotation_group=attB"
     CDS             1036..14145
                     /codon_start=1
                     /label=HUWE1
                     /note="/created_by=mhunkeler"
                     /translation="MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWN
                     IGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLI
                     EYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRL
                     QHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLH
                     YIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARL
                     HAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERT
                     PKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAG
                     GEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIY
                     RLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKK
                     AIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSL
                     QDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLL
                     SPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRD
                     PKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQ
                     QVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLP
                     IDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLL
                     RELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLG
                     LSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKR
                     SDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQ
                     IKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTK
                     LLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNAL
                     FETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAK
                     LPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGE
                     PVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTS
                     TMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACR
                     KEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRV
                     CDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATL
                     PQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQT
                     PKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTI
                     DSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNG
                     QELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVT
                     VLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNG
                     FTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVL
                     GPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPS
                     PLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYR
                     SLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRA
                     SEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLA
                     FVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAES
                     TEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARV
                     PHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSS
                     SNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVEN
                     ELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMN
                     ILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSE
                     MELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHA
                     DHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLG
                     PSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTL
                     SSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEE
                     ETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESK
                     ETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEA
                     ETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAA
                     GSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAIS
                     GADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPT
                     RTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDT
                     PMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLF
                     GHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTL
                     LRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQ
                     RSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSM
                     GSGNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQ
                     RTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPV
                     SAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEA
                     QANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAAS
                     TTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTS
                     HSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYN
                     LEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLP
                     SMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQ
                     LEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIA
                     SDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEA
                     FFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHI
                     SSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQ
                     ELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLL
                     REWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDN
                     RLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQE
                     FGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLI
                     SIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQ
                     FVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLR
                     HMLLLAIQECSEGFGLA"
     misc_feature    complement(14161..14181)
                     /direction=LEFT
                     /label=attB2
                     /label=nonstandard type: attB2
                     /note="/annotation_group=attB"
     promoter        14860..15190
                     /label=SV40 promoter
                     /note="<html><body>SV40 enhancer and early 
                     promoter</body></html>"
     rep_origin      15041..15176
                     /label=SV40 ori
                     /note="<html><body>SV40 origin of
                     replication</body></html>"
     promoter        complement(15229..15247)
                     /label=T7 promoter
                     /note="<html><body>promoter for bacteriophage T7 RNA 
                     polymerase</body></html>"
     primer_bind     complement(15261..15277)
                     /label=M13 rev
                     /note="<html><body>common sequencing primer, one of
                     multiple similar variants</body></html>"
     protein_bind    15285..15301
                     /label=lac operator
                     /bound_moiety="<html><body><i>lac</i> repressor encoded by 
                     <i>lacI</i></body></html>"
                     /note="<html><body>The <i>lac</i> repressor binds to the 
                     <i>lac</i> operator to inhibit transcription in <i>E. 
                     coli</i>. This inhibition can be relieved by adding lactose
                     or isopropyl-beta-D-thiogalactopyranoside 
                     (IPTG).</body></html>"
     promoter        complement(join(15334..15339,15316..15333,15309..15315))
                     /label=lac promoter
                     /note="<html><body>promoter for the <i>E. coli lac</i> 
                     operon</body></html>"
     protein_bind    15354..15375
                     /label=CAP binding site
                     /bound_moiety="<html><body><i>E. coli</i> catabolite
                     activator protein</body></html>"
                     /note="<html><body>CAP binding activates transcription in
                     the presence of cAMP.</body></html>"
     rep_origin      complement(15663..16251)
                     /direction=LEFT
                     /label=ori
                     /note="<html><body>high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication</body></html>"
     CDS             complement(join(17214..17282,16422..17213))
                     /codon_start=1
                     /gene="<html><body><i>bla</i></body></html>"
                     /product="<html><body>beta-lactamase</body></html>"
                     /label=AmpR
                     /note="<html><body>confers resistance to ampicillin, 
                     carbenicillin, and related antibiotics</body></html>"
                     /translation="MPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMS
                     TFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITM
                     SDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMAT
                     TLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIA
                     ALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*MSIQHFRVALIPFFAAF
                     CLPVFA"
     promoter        complement(17283..17387)
                     /gene="<html><body><i>bla</i></body></html>"
                     /label=AmpR promoter
     rep_origin      17414..17870
                     /direction=RIGHT
                     /label=f1 ori
                     /note="<html><body>f1 bacteriophage origin of replication;
                     arrow indicates direction of (+) strand 
                     synthesis</body></html>"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccca
      121 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctgat ctaatcaata
      181 ttggccatta gccatattat tcattggtta tatagcataa atcaatattg gctattggcc
      241 attgcatacg ttgtatccat atcataatat gtacatttat attggctcat gtccaacatt
      301 accgccatgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      361 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      421 cgaccgccca gcgacccccg cccgttgacg tcaatagtga cgtatgttcc catagtaacg
      481 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      541 gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa
      601 tggcccgcct agcattatgc ccagtacatg accttacggg agtttcctac ttggcagtac
      661 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg
      721 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      781 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg
      841 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      901 gtgaaccgtc agaattgatc taccatggac tacaaagacg atgacgacaa gcttgcggcc
      961 gcgaattcat cgatagatct gatatcaaca agtttgtaca aaaaagcagg ctttaaagga
     1021 accaattcag tcgacatgaa agtagacagg actaaactga agaagacacc tactgaggct
     1081 cctgcagact gcagagcctt aatagacaaa ctcaaagttt gtaatgatga gcaacttctc
     1141 ttggaactgc agcagatcaa aacatggaac attggaaagt gcgagttata tcactgggtg
     1201 gacctgttgg accgcttcga tggaatactg gcagatgctg gacagacagt ggagaatatg
     1261 tcatggatgc tcgtatgtga taggccagaa agagagcaac tgaaaatgct tctcttggct
     1321 gtgttgaact tcacagcctt gctcattgag tacagctttt cccggcatct gtacagttcc
     1381 atagagcatt tgacaacttt attggcttcc tctgatatgc aagtggtgct ggcagtcctc
     1441 aatctcctat atgtatttag caaaagatca aactacatca ctcgtctggg atctgacaag
     1501 aggaccccgc tgctaactcg gctacaacat ttggcagaga gctggggtgg aaaggagaat
     1561 ggctttggac ttgcagaatg ttgcagagac ttgcatatga tgaaatatcc acccagtgca
     1621 actacactac actttgaatt ctatgcagat cctggggccg aggtcaaaat tgagaaaagg
     1681 acaactagta acacactaca ttatattcac atagagcaac ttgacaagat ttcagaaagc
     1741 ccttctgaaa tcatggaatc tcttaccaaa atgtacagca ttcctaagga taagcagatg
     1801 ctgttattta cacacatacg actggcccat ggcttttcta atcacaggaa gcgattgcag
     1861 gcagttcagg ccagactgca tgcaatatct atattagtgt attccaatgc cttgcaggaa
     1921 tcagcaaaca gtatcttgta taatggcttg atagaggagt tagtggacgt gctgcaaata
     1981 acggataagc agcttatgga gattaaagca gcttctttac gaacattaac atcaattgtc
     2041 cacttggaga gaactcccaa actcagcagt attattgact gtactggaac tgcctcctac
     2101 catggatttt tgccagtgct tgtaaggaac tgtatccagg ccatgattga tccttccatg
     2161 gatccatacc ctcaccagtt tgccactgct ctcttctctt ttttatacca tctggccagc
     2221 tacgatgctg gtggtgaagc cttggtctcc tgtggaatga tggaagcctt attgaaggtc
     2281 ataaagtttc ttggcgatga acaggaccag ataacatttg tcaccagagc cgtcagagtg
     2341 gttgacctta tcaccaacct ggatatggca gcttttcaat cccatagtgg actttctatc
     2401 ttcatttata gacttgagca tgaagtagat ctgtgccgaa aagaatgtcc gtttgtgatc
     2461 aagccaaaga tccagagacc caatactaca caagaaggag aggaaatgga aactgatatg
     2521 gatggagtcc agtgtattcc acaacgagca gcacttctga aatccatgtt gaatttcctc
     2581 aagaaggcca tccaagaccc tgctttctca gatggcatac gacatgtgat ggatggttct
     2641 ctgcctacct ccctgaaaca catcatcagc aatgcagaat actatggccc atcactcttc
     2701 ctcctagcta ctgaagtggt gactgtgttt gtatttcaag aaccatcact gctctcctca
     2761 ctccaggaca atggattgac agatgtcatg ctgcatgcac tgcttatcaa agatgttcct
     2821 gctacccgtg aagtccttgg ctccctccca aatgtattca gtgcactctg tttgaatgcc
     2881 cgaggtcttc agtcttttgt tcagtgtcag ccttttgaac gcctcttcaa agttcttctg
     2941 tctccagatt acctcccagc catgcggagg aggagaagtt ctgatcccct tggggatact
     3001 gcatccaacc tggggagtgc tgtcgatgag ctcatgagac atcagcccac ccttaaaaca
     3061 gatgcaacga ctgccatcat caagttactt gaagaaatct gtaatcttgg aagggacccc
     3121 aaatacatct gtcagaagcc atcgatccag aaggcagatg gcactgccac tgctcctccc
     3181 ccaaggtcta atcatgccgc agaagaagcc tctagtgagg atgaggagga agaggaagta
     3241 caggccatgc agagctttaa ttctacccag caaaatgaaa ctgagcctaa tcagcaggtt
     3301 gttggtacag aggaacgtat tcctattccc ctcatggatt acatccttaa tgtgatgaaa
     3361 tttgtggaat ctattctgag caacaataca acagatgacc actgccagga atttgtgaat
     3421 cagaaaggac tgttgccttt ggttaccatt ttgggtcttc ccaatctgcc cattgacttt
     3481 cccacatctg ctgcctgtca ggctgttgca ggtgtctgca aatccatatt gacactgtca
     3541 catgaaccca aagtccttca agagggtctc cttcagttgg actccatcct ctcctccctg
     3601 gagcccttac accgccccat tgaatcccct gggggctcag tgttgttgcg agaactggct
     3661 tgcgcaggca atgttgctga tgctaccctc tcagcccagg ccacacctct gctgcatgca
     3721 ctcactgctg cccatgccta catcatgatg tttgttcata cttgcagagt tggacagagt
     3781 gaaattcgtt ccatctccgt aaaccagtgg ggctctcaat tgggtctgag tgttttgagc
     3841 aagctgagcc agttatactg ttccctggtg tgggaaagca ctgtcctcct ctctctgtgt
     3901 accccaaaca gcctaccatc tgggtgtgaa tttggccagg cagatatgca gaaactggtt
     3961 ccaaaggatg agaaggcagg tacgacccag ggcggaaaaa gatcagatgg ggaacaggat
     4021 ggagcagctg gaagtatgga tgcttctacc cagggcttat tagaaggcat tgggctagat
     4081 ggtgacacat tggctcccat ggagacagat gaacctactg cttcagactc taagggcaaa
     4141 tctaaaatca caccagcaat ggctgccaga attaagcaaa tcaagccttt gttatcagct
     4201 tcctccagat taggccgagc acttgctgag ctatttggac ttcttgttaa actttgtgtg
     4261 ggatctcctg tccgccagag aaggagccat catgctgcca gcaccactac agcaccgaca
     4321 cctgccgcgc gatcaacagc ctcagctctc actaagctct tgactaaggg gttatcttgg
     4381 cagcccccac catatacacc tactccccga ttcaggctga cattcttcat ctgttcagtt
     4441 ggtttcacat ccccaatgct gtttgatgag aggaagtatc cctaccacct catgctgcaa
     4501 aaatttctct gctccggagg ccacaatgct ctttttgaaa ctttcaactg ggctctgtcc
     4561 atgggaggta aagttcctgt ttctgaggga ttggaacact cagacttgcc tgatggcaca
     4621 ggagaattcc tagatgcctg gcttatgctg gtggagaaga tggtgaatcc caccacggtg
     4681 cttgaatctc cacattcgct gcctgccaaa ttgcctggag gtgtccagaa ctttccccag
     4741 ttcagtgcac tgcgcttcct tgtggtaact cagaaagcag cctttacttg catcaaaaac
     4801 ttatggaacc ggaaacccct gaaggtatat ggtggacgaa tggctgaatc gatgctggcc
     4861 attctatgcc acatcctccg aggagaacct gtgattcgag agagactaag caaggagaag
     4921 gaggggtctc gaggagaaga ggatacaggg caagaggaag gtggctcccg ccgggaacct
     4981 caagtcaacc agcaacaact gcaacagctc atggacatgg gcttcacaag ggaacatgca
     5041 atggaggcac tgttgaacac cagcaccatg gagcaggcca cagagtacct tttaacccac
     5101 cctcctccaa tcatgggagg agttgttcgg gatctcagca tgtctgaaga ggaccagatg
     5161 atgagagcaa ttgctatgtc tctgggacag gatattccaa tggatcaaag ggcagagtca
     5221 cctgaggaag ttgcttgccg gaaggaggaa gaggaacgga aagctcggga aaagcaggag
     5281 gaggaagagg ctaaatgtct agagaagttc caggatgctg acccgttgga acaagatgag
     5341 ctccacactt tcacagatac tatgttgcca ggctgcttcc accttcttga tgagctgcca
     5401 gacacagtat accgtgtgtg tgacctgatc atgacagcaa tcaaacgtaa tggagcagat
     5461 tatcgtgaca tgattctgaa gcaagtagtc aatcaggtgt gggaagctgc tgatgtattg
     5521 atcaaagctg ctcttcccct gacaacaagt gacacaaaaa ccgtgtcaga gtggataagt
     5581 cagatggcca cactgcccca ggcctccaat ttggctacta gaatcttgct tttaacgcta
     5641 ctttttgagg agttgaagct accttgtgct tgggtggttg aatcaagtgg catccttaat
     5701 gtcctaatca aactcttgga agtggttcag ccctgcctcc aggcagccaa ggagcagaag
     5761 gaagtccaga ccccaaagtg gatcacacca gtgttgctcc tgattgattt ctatgaaaag
     5821 acagccatct cctcaaaaag gagagcccag atgactaagt acctgcaatc caacagcaac
     5881 aactggcgct ggtttgatga tcgctctggg cgttggtgta gttacagtgc aagcaacaat
     5941 agcactattg attctgcctg gaaatctgga gagacaagcg tgcgattcac tgcaggccga
     6001 agaagataca cggtccaatt cactacaatg gtgcaggtta atgaggaaac agggaaccga
     6061 cgccctgtga tgctgactct cctcagggta cctcggctga ataaaaattc aaaaaacagc
     6121 aatggacagg aactagagaa gacgctggaa gaaagcaaag aaatggatat caaacgtaaa
     6181 gaaaataaag gcaatgatac ccctttggcc ctagagagta caaacactga aaaggagaca
     6241 agcctggagg aaacaaaaat cggggagatc ctgatccagg gcttgacaga agatatggtg
     6301 actgttttaa tccgggcctg cgtgagcatg ctgggagtcc ctgtggaccc agatactttg
     6361 catgccaccc ttcgtctctg tctgaggctc acccgggacc acaaatatgc catgatgttt
     6421 gcagaactga agagtacccg catgatcttg aatttgaccc agagctcagg cttcaatggg
     6481 tttactcccc tggtcaccct tctcttaaga cacatcattg aggacccctg tacccttcgt
     6541 cataccatgg aaaaggttgt tcgctcagca gctacaagtg gagctggtag cactacctct
     6601 ggtgttgtgt ctggcagcct cggctctcgg gagatcaact acatccttcg tgtccttggg
     6661 ccagccgcat gccgcaatcc agacatattc acagaagtgg ccaactgctg tatccgcatc
     6721 gcccttcctg cccctcgagg ctcaggaact gcttcagatg atgaatttga gaatcttaga
     6781 attaaaggcc ctaatgctgt acagctggtg aagaccaccc ctttgaagcc ctcacctctg
     6841 cctgtcatcc ctgatactat caaggaagtg atctatgata tgctgaatgc tctggctgca
     6901 taccatgctc cagaggaagc cgataaatct gatcctaaac ctggggttat gacccaagag
     6961 gttggccagc tcctgcaaga catgggtgat gatgtatacc agcagtaccg gtcacttacg
     7021 cgtcagagca gtgactttga tacgcagtca ggtttttcca ttaatagtca ggtctttgct
     7081 gcagatggtg cctccactga gacttccgca tctgggacct cccaaggaga ggcttcaact
     7141 ccagaggagt ctcgagatgg gaagaaagat aaagaagggg accgggcctc tgaggaaggc
     7201 aaacagaaag gcaagggcag caaaccttta atgcctacct ccactatcct tcgtcttctg
     7261 gcagagttgg tgaggtccta tgttggtatt gctaccctga ttgccaacta cagctacact
     7321 gtgggccagt ctgaactgat caaagaggac tgcagtgtgc tagcttttgt tctggaccac
     7381 ctgctcccac atacccagaa tgcagaagac aaggacaccc ctgccttggc ccgcctgttc
     7441 ctcgcaagcc tggctgctgc agggagtggc acagatgccc aggtggccct agtgaatgaa
     7501 gtaaaagcag cccttggacg ggcactggct atggctgaga gtacagagaa acatgccagg
     7561 cttcaggcag tgatgtgtat catcagtact atcatggagt cctgcccctc cacctccagc
     7621 ttctacagca gtgccacagc gaagacccag cacaatggca tgaacaacat cattcggctt
     7681 ttcctgaaga agggactggt taatgacctg gccagagtac ctcacagctt agacctgtcc
     7741 agtcccaaca tggccaacac agtcaatgct gctctgaagc ctttggaaac actttcccgg
     7801 attgtgaacc agcccagtag cctttttggc agcaagagtg cttctagcaa gaacaagtct
     7861 gagcaggatg cccaaggagc ctctcaagat tccagtagca accagcagga cccaggcgag
     7921 cctggggaag cagaagtgca ggaggaggat catgatgtca ctcagacaga ggtggcagat
     7981 ggggatatca tggatgggga ggctgaaacc gactcagtgg tgattgctgg gcagcctgag
     8041 gtgctcagtt cacaagagat gcaggttgag aatgagctgg aggacctgat agatgagttg
     8101 cttgagaggg atggcggatc tgggaacagt acaattatag tgagcagaag tggagaggat
     8161 gaatcacaag aggacgtgct gatggatgaa gctccttcca acctcagcca agcttccacc
     8221 ttgcaggcca accgagaaga ttccatgaat atcctggacc ctgaggatga ggaggagcac
     8281 actcaggaag aggacagcag tggcagtaac gaggatgagg atgatagtca ggatgaagag
     8341 gaggaggagg aggaagatga ggaagatgat caggaggatg atgaaggtga agagggagat
     8401 gaagacgatg acgacgatgg ctctgagatg gaattggatg aggattatcc tgatatgaac
     8461 gcttctccct tggtccgatt tgagcgcttt gaccgggagg atgatctcat cattgagttt
     8521 gacaacatgt tctccagtgc tacagacatc cccccatccc caggaaatat ccctaccacc
     8581 catccactga tggtgcgcca tgcagaccac agttctctga cactgggcag tggctcttca
     8641 acaactcgtc tcacccaggg catcgggcgc agtcagagga ccctaaggca gctgacggcc
     8701 aatactggcc acaccattca tgttcactac cctgggaatc gccagcccaa ccctcctctt
     8761 atactgcaga ggttgcttgg tccctcagct gctgctgaca tccttcagct gagcagcagc
     8821 cttcccctac aaagccgggg tcgggcccgc ctcctggtag gcaacgatga cgtccacatc
     8881 atcgcccgtt ctgatgatga gctgctggat gactttttcc atgatcagag cacagctacc
     8941 agccaagcag gaaccctgtc cagcatcccc acagccctga cccgctggac agaagaatgc
     9001 aaagttctcg atgctgagag catgcatgac tgtgtttcag tggttaaagt gtccattgtc
     9061 aatcacctgg aattcctgag ggatgaggag ctggaagaaa ggcgagagaa gcgcaggaaa
     9121 caactggctg aggaagaaac aaagataact gataaaggca aagaagataa ggagaacagg
     9181 gatcagagtg cacagtgtac tgcatctaag tcaaatgact ccactgaaca gaatctctca
     9241 gatgggacgc ctatgcctga cagctaccca acaaccccat cttcaactga tgcagctaca
     9301 tctgagtcca aggagaccct tggcactctg caatcctcac aacagcaacc aacactccca
     9361 accccaccag ctttgggaga ggttcctcag gagctgcagt ctccagctgg agaagggggc
     9421 agctctacac agctattgat gcctgtagag ccagaggaat tgggtcccac aaggccaagt
     9481 ggggaagcag aaacaactca gatggagtta tccccagctc ccactataac ctcactttcc
     9541 ccagagagag ctgaggattc tgatgcactg acggctgtca gcagtcagct agaaggctct
     9601 cctatggata caagcagcct ggcttcctgt accttagagg aggctgtggg tgacacttca
     9661 gcagctggca gttctgagca gcccagagca ggcagctcca ctcctgggga tgccccacca
     9721 gctgtggcgg aagtgcaagg caggagtgat gggtcagggg aatctgccca gccacctgag
     9781 gacagctccc cacctgcatc ctctgagagc tcttccacca gagattctgc cgtggccatt
     9841 tctggcgccg attctcgggg aatactggaa gagccactgc catctacctc tagtgaggaa
     9901 gaagatcctc ttgccggtat atcattgccc gagggggtag acccgtcttt cctcgccgcc
     9961 ctcccggacg atattcggag agaagtactt caaaatcaac tcggaattcg gccccccact
    10021 cgcactgccc cgagtactaa tagctcagcg cccgccgttg tcgggaatcc aggagtcact
    10081 gaagtgagtc cggagttcct tgcagcactc ccgccggcta ttcaggaaga agtccttgcg
    10141 caacagcgag cggaacaaca gaggcgagaa cttgcccaaa acgcatcttc agacactcca
    10201 atggaccctg tcacgttcat acagaccctt ccctccgact tgcgacgatc cgtgctggaa
    10261 gacatggaag attctgtact tgccgtaatg ccgcctgata ttgcggcaga agcacaagct
    10321 cttcgaaggg aacaagaagc aaggcagcga cagttgatgc atgagaggct cttcggtcac
    10381 tcctcaacct ccgcgttgtc tgccatactc agatcacccg cgttcacaag ccgcctctca
    10441 ggaaatcggg gcgtccagta caccagattg gccgtccaac gaggcggcac gtttcaaatg
    10501 ggcgggtcta gctcacataa taggccaagc ggctcaaatg tagatactct gctgcgactt
    10561 cggggacgac tgctgttgga tcacgaagcg ctctcctgtc tcttggttct gctcttcgtt
    10621 gatgagccta agctcaacac aagtaggctc catcgcgtac tgcggaattt gtgctatcat
    10681 gcgcagacac gccattgggt aattaggagc ttgctttcaa tcctgcagcg aagttcagaa
    10741 tccgaacttt gcatagaaac accaaagctt accacttccg aggaaaaggg gaaaaagtct
    10801 tcaaaatctt gcggtagcag ctcacatgaa aatcgcccgc tcgatctttt gcacaaaatg
    10861 gaaagcaagt ctagtaatca gctctcctgg ctttccgttt ccatgggatc tgggaatata
    10921 tttcagattc agagatctgg tggccgcaag catacagaaa agcatgcttc tggtggaagt
    10981 acggtacaca tacaccctca ggcagcccct gtcgtatgca ggcacgtgtt ggacacactc
    11041 atccagctcg ctaaggtctt tccctcccat tttactcagc aacgaacgaa ggaaaccaat
    11101 tgcgagagtg accgcgaaag agggaacaaa gcgtgctctc cgtgttcttc ccagtcttca
    11161 agtagcggca tttgtactga tttttgggat ctgcttgtga aactggataa catgaacgtt
    11221 tccagaaagg ggaaaaactc cgtcaaatct gtcccggtgt ctgcgggagg cgagggtgag
    11281 acatcccctt actctctgga ggcatcaccc ctgggacaac ttatgaatat gctcagccac
    11341 cctgtgatac gaagatcctc acttctgacg gaaaagctgc tcaggctgct ttcactgatt
    11401 tccatagcac ttcctgagaa caaggtgagt gaggcacagg caaattccgg gtctggagct
    11461 tcttccacaa cgactgcgac ctccacaacc agcacaacaa ctacgactgc tgcgtctacc
    11521 acccctactc cccctacggc gcctactccc gtcacatccg cgccggccct cgtagccgct
    11581 acggcgataa gtacgatcgt cgttgcggcc agtactacgg tcaccacacc gaccacagcg
    11641 accacgacgg tcagtattag tcctacgact aaggggtcaa agagccctgc taaagtgtcc
    11701 gacggaggat cctcctcaac agattttaaa atggtgtcct ctggacttac ggaaaaccag
    11761 ctgcagcttt ccgtagaggt acttacctca cattcctgca gcgaggaagg actcgaagac
    11821 gctgcgaatg tgctcctgca gctgagtaga ggagattccg gtaccaggga taccgttctg
    11881 aaattgcttc ttaatggcgc caggcacctc gggtacactc tgtgcaagca aattggtact
    11941 ctgttggccg aactccgaga atacaatctg gagcagcaac gacgggcgca gtgtgaaaca
    12001 ctgagtcccg acgggcttcc tgaggaacag ccgcagacaa cgaaacttaa gggtaaaatg
    12061 cagagtcggt tcgacatggc agaaaatgtc gtgatagtgg cttctcagaa acgccctctc
    12121 gggggaaggg aactgcagct gcccagtatg tccatgctta cttccaagac aagcacccag
    12181 aagttttttt tgagagtact tcaagtcata attcaattga gagacgatac tcgccgagcc
    12241 aacaagaaag ccaaacaaac gggaaggctc ggttcttctg gtctgggctc agcatcatcc
    12301 atccaggcgg ccgtgcggca acttgaagca gaggcagacg ccatcataca aatggtacgt
    12361 gagggtcaaa gggcgcggag acagcaacaa gcagcaacgt cggagtctag ccagtcagag
    12421 gcgtctgtcc ggagggagga atcacccatg gatgtggacc agccatctcc cagtgctcaa
    12481 gatactcaat ccattgcctc cgatggaacc ccacaggggg agaaggaaaa ggaagaaaga
    12541 ccacctgagt tacccctgct cagcgagcag ctgagtttgg acgagctgtg ggacatgctt
    12601 ggggagtgtc taaaggaact agaggaatcc catgaccagc atgcggtgct agtgctacag
    12661 cctgctgtcg aggccttctt tctggtccat gccacagagc gggagagcaa gcctcctgtc
    12721 cgagacaccc gtgagagcca gctggcacac atcaaggacg agcctcctcc actctcccct
    12781 gcccccttaa ccccagccac gccttcctcc cttgacccat tcttctcccg ggagccctca
    12841 tctatgcaca tctcctcaag cctgccccct gacacacaga agttccttcg ctttgcagag
    12901 actcaccgca ctgtgttaaa ccagatccta cggcagtcca cgacccacct tgctgatggg
    12961 ccttttgctg tcctggtaga ctacattcgt gtcctcgact ttgatgtcaa gcgcaaatat
    13021 ttccgccaag agctggagcg tttagatgag gggctccgga aagaagacat ggctgtgcat
    13081 gtccgtcgtg accatgtgtt tgaagactcc tatcgtgagc tgcatcgcaa atcccccgaa
    13141 gaaatgaaga atcgattgta tatagtattt gaaggagaag aagggcagga tgctggcggg
    13201 ctcctgcggg agtggtatat gatcatctct cgagagatgt ttaaccctat gtatgccttg
    13261 ttccgtacct cacctggtga tcgagtcacc tacaccatca atccatcttc ccactgcaac
    13321 cccaaccatc tgtcctattt taaatttgtc ggacgcattg tggccaaagc tgtatatgac
    13381 aaccgtcttc tggagtgcta ctttactcga tccttttaca aacacatctt gggcaagtca
    13441 gtcagatata cagatatgga gagtgaagat taccacttct accaaggtct ggtttatctg
    13501 ctggaaaatg atgtctccac actaggctat gacctcacct tcagcactga ggtccaagag
    13561 tttggagttt gtgaagttcg tgacctcaaa cccaatgggg ccaacatctt ggtaacagag
    13621 gagaataaga aggagtatgt acacctggta tgccagatga gaatgacagg agccatccgc
    13681 aagcagttgg cggctttctt agaaggcttc tatgagatca ttccaaagcg cctcatttcc
    13741 atcttcactg agcaggagtt agagctgctt atatcaggac tgcccaccat tgacatcgat
    13801 gatctgaaat ccaacactga ataccacaag taccagtcca actctattca gatccagtgg
    13861 ttctggagag cattgcgttc tttcgatcaa gctgaccgtg ccaagttcct ccagtttgtc
    13921 acgggtactt ccaaggtacc cctgcaaggc tttgctgccc tcgaaggcat gaatggcatt
    13981 cagaagtttc agatccatcg agatgacagg tccacagatc gcctgccttc agctcacaca
    14041 tgttttaatc agctggatct gcctgcctat gagagctttg agaagctccg ccacatgcta
    14101 ctgttggcta tccaggagtg ctctgaaggc tttgggctgg cctaataagc ggccgcgacc
    14161 cagctttctt gtacaaagtg gttgatatcg gtaccagtcg actctagagg atcccgggtg
    14221 gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc
    14281 caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta
    14341 taatattatg gggtggaggg ggggtggtat ggagcaaggg gcaagttggg aagacaacct
    14401 gtagggcctg cggggtctat tgggaaccaa gctggagtgc agggcacaat cttggctcac
    14461 tgcaatctcc gctctgggtt caagcgattc tcctgcctca cctccgagtt gtgggattcc
    14521 aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca
    14581 tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca
    14641 aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg attttaaaat
    14701 aactatacca gcaggaggac gtccagacac agcataggct acctgccatg gcccaaccgg
    14761 tgggacattt gagttgcttg cttggcactg tcctctcatg cgttgggtcc actcagtaga
    14821 tgcctgttga attgggtacg cggccagctt ctgtggaatg tgtgtcagtt agggtgtgga
    14881 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
    14941 accaggtgtg gaaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
    15001 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
    15061 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
    15121 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
    15181 cttttgcaaa aagctcctcg aggaactgaa aaaccagaaa gttaattccc tatagtgagt
    15241 cgtattaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    15301 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    15361 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    15421 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    15481 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    15541 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    15601 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    15661 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    15721 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    15781 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    15841 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    15901 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    15961 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    16021 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    16081 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    16141 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    16201 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    16261 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    16321 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    16381 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    16441 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    16501 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    16561 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    16621 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    16681 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    16741 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    16801 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    16861 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    16921 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    16981 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    17041 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    17101 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    17161 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    17221 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    17281 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    17341 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    17401 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
    17461 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
    17521 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta
    17581 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
    17641 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    17701 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
    17761 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
    17821 taacaaaaat ttaacgcgaa ttttaacaaa atattaaacg tttacaattt
//
