LOCUS       Exported               17822 bp DNA     circular SYN 01-JUN-2022
DEFINITION  Isoform1, ∆(169-189), patient I, Expression clone.
ACCESSION   urn.local.mhunkeler.8h-evumryr
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 7, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..17822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     143..159
                     /label=M13 fwd
                     /note="<html><body>common sequencing primer, one of
                     multiple similar variants</body></html>"
     enhancer        312..690
                     /label=CMV enhancer
                     /note="<html><body>human cytomegalovirus immediate early 
                     enhancer</body></html>"
     promoter        691..894
                     /label=CMV promoter
                     /note="<html><body>human cytomegalovirus (CMV) immediate
                     early promoter</body></html>"
     CDS             928..951
                     /codon_start=1
                     /product="<html><body>FLAG(R) epitope tag, followed by an 
                     enterokinase cleavage site</body></html>"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    989..1009
                     /label=attB1
                     /label=nonstandard type: attB1
                     /note="/annotation_group=attB"
     CDS             1036..14097
                     /codon_start=1
                     /label=Huwe1_cds
                     /note="/Original_Translation=MKVDRTKLKKTPTEAPADCRALIDKLKVCN
                     DEQLL 
                     LELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLL 
                     LAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRL 
                     GSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAE 
                     VKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGF 
                     SNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKA 
                     ASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFA 
                     TALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITN 
                     LDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDGVQ 
                     CIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLL 
                     ATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNA 
                     RGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTL 
                     KTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEE 
                     EEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDH 
                     CQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQ 
                     LDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMM 
                     FVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSG 
                     CEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAP 
                     METDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPV 
                     RQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGF 
                     TSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGT 
                     GEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCI 
                     KNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGS 
                     RREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSM 
                     SEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQD 
                     ADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVV 
                     NQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLP 
                     CAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSK 
                     RRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYT 
                     VQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKEN 
                     KGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTL 
                     HATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCT 
                     LRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANC 
                     CIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDM 
                     LNALAAYHAPEEDKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFS 
                     INSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLM 
                     PTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAE 
                     DKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCI 
                     ISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMA 
                     NTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGE 
                     AEVQEEDHDVTHTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELL 
                     ERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEE 
                     HTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYP 
                     DMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTL 
                     GSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAAD 
                     ILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPT 
                     ALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKI 
                     TDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETL 
                     GTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAET 
                     TQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAG 
                     SSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAIS 
                     GADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPP 
                     TRTAPSTNSSAPAVVGNPGVTEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSD 
                     LRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILR 
                     SPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHE 
                     ALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIET 
                     PKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNI 
                     FQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKE 
                     TNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAG 
                     GEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQA 
                     NSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAAST 
                     TVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTS 
                     HSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREY 
                     NLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQ 
                     LPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAA 
                     VRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDT 
                     QSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQ 
                     PAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSRE 
                     PSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDV 
                     KRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEE 
                     GQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGR 
                     IVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGY 
                     DLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLE 
                     GFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALR 
                     SFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQ 
                     LDLPAYESFEKLRHMLLLAIQECSEGFGLA*"
                     /translation="MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWN
                     IGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLI
                     EYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRL
                     QHLAEKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESL
                     TKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILY
                     NGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPV
                     LVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLG
                     DEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKI
                     QRPNTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPT
                     SLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPAT
                     REVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTA
                     SNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPP
                     PRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVM
                     KFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILT
                     LSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPL
                     LHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVL
                     LSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLE
                     GIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGL
                     LVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRL
                     TFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLE
                     HSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQ
                     KAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTG
                     QEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVV
                     RDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLE
                     KFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILK
                     QVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELK
                     LPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISS
                     KRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYT
                     VQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENK
                     GNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHA
                     TLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRH
                     TMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRI
                     ALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALA
                     AYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQV
                     FAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTIL
                     RLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPAL
                     ARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESC
                     PSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKP
                     LETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDV
                     TQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTI
                     IVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNE
                     DEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERF
                     DREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIG
                     RSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGR
                     ARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAES
                     MHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQC
                     TASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPAL
                     GEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERA
                     EDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVA
                     EVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEED
                     PLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTE
                     VSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLE
                     DMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRL
                     SGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLL
                     FVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKG
                     KKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKH
                     TEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNK
                     ACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEAS
                     PLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATS
                     TTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSIS
                     PTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLL
                     QLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDG
                     LPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFL
                     RVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQ
                     RARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPP
                     ELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVR
                     DTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAE
                     THRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAV
                     HVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMY
                     ALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHIL
                     GKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANI
                     LVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLP
                     TIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAAL
                     EGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGL
                     A"
     misc_feature    complement(14113..14133)
                     /direction=LEFT
                     /label=attB2
                     /label=nonstandard type: attB2
                     /note="/annotation_group=attB"
     promoter        14812..15142
                     /label=SV40 promoter
                     /note="<html><body>SV40 enhancer and early 
                     promoter</body></html>"
     rep_origin      14993..15128
                     /label=SV40 ori
                     /note="<html><body>SV40 origin of
                     replication</body></html>"
     promoter        complement(15181..15199)
                     /label=T7 promoter
                     /note="<html><body>promoter for bacteriophage T7 RNA 
                     polymerase</body></html>"
     primer_bind     complement(15213..15229)
                     /label=M13 rev
                     /note="<html><body>common sequencing primer, one of
                     multiple similar variants</body></html>"
     protein_bind    15237..15253
                     /label=lac operator
                     /bound_moiety="<html><body><i>lac</i> repressor encoded by 
                     <i>lacI</i></body></html>"
                     /note="<html><body>The <i>lac</i> repressor binds to the 
                     <i>lac</i> operator to inhibit transcription in <i>E. 
                     coli</i>. This inhibition can be relieved by adding lactose
                     or isopropyl-beta-D-thiogalactopyranoside 
                     (IPTG).</body></html>"
     promoter        complement(join(15286..15291,15268..15285,15261..15267))
                     /label=lac promoter
                     /note="<html><body>promoter for the <i>E. coli lac</i> 
                     operon</body></html>"
     protein_bind    15306..15327
                     /label=CAP binding site
                     /bound_moiety="<html><body><i>E. coli</i> catabolite
                     activator protein</body></html>"
                     /note="<html><body>CAP binding activates transcription in
                     the presence of cAMP.</body></html>"
     rep_origin      complement(15615..16203)
                     /direction=LEFT
                     /label=ori
                     /note="<html><body>high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication</body></html>"
     CDS             complement(join(17166..17234,16374..17165))
                     /codon_start=1
                     /gene="<html><body><i>bla</i></body></html>"
                     /product="<html><body>beta-lactamase</body></html>"
                     /label=AmpR
                     /note="<html><body>confers resistance to ampicillin, 
                     carbenicillin, and related antibiotics</body></html>"
                     /translation="MPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMS
                     TFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITM
                     SDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMAT
                     TLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIA
                     ALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*MSIQHFRVALIPFFAAF
                     CLPVFA"
     promoter        complement(17235..17339)
                     /gene="<html><body><i>bla</i></body></html>"
                     /label=AmpR promoter
     rep_origin      17366..17822
                     /direction=RIGHT
                     /label=f1 ori
                     /note="<html><body>f1 bacteriophage origin of replication;
                     arrow indicates direction of (+) strand 
                     synthesis</body></html>"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccca
      121 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctgat ctaatcaata
      181 ttggccatta gccatattat tcattggtta tatagcataa atcaatattg gctattggcc
      241 attgcatacg ttgtatccat atcataatat gtacatttat attggctcat gtccaacatt
      301 accgccatgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      361 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      421 cgaccgccca gcgacccccg cccgttgacg tcaatagtga cgtatgttcc catagtaacg
      481 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      541 gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa
      601 tggcccgcct agcattatgc ccagtacatg accttacggg agtttcctac ttggcagtac
      661 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg
      721 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      781 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg
      841 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      901 gtgaaccgtc agaattgatc taccatggac tacaaagacg atgacgacaa gcttgcggcc
      961 gcgaattcat cgatagatct gatatcaaca agtttgtaca aaaaagcagg ctttaaagga
     1021 accaattcag tcgacatgaa agtagacagg actaaactga agaagacacc tactgaggct
     1081 cctgcagact gcagagcctt aatagacaaa ctcaaagttt gtaatgatga gcaacttctc
     1141 ttggaactgc agcagatcaa aacatggaac attggaaagt gcgagttata tcactgggtg
     1201 gacctgttgg accgcttcga tggaatactg gcagatgctg gacagacagt ggagaatatg
     1261 tcatggatgc tcgtatgtga taggccagaa agagagcaac tgaaaatgct tctcttggct
     1321 gtgttgaact tcacagcctt gctcattgag tacagctttt cccggcatct gtacagttcc
     1381 atagagcatt tgacaacttt attggcttcc tctgatatgc aagtggtgct ggcagtcctc
     1441 aatctcctat atgtatttag caaaagatca aactacatca ctcgtctggg atctgacaag
     1501 aggaccccgc tgctaactcg gctacaacat ttggcagaga aatatccacc cagtgcaact
     1561 acactacact ttgaattcta tgcagatcct ggggccgagg tcaaaattga gaaaaggaca
     1621 actagtaaca cactacatta tattcacata gagcaacttg acaagatttc agaaagccct
     1681 tctgaaatca tggaatctct taccaaaatg tacagcattc ctaaggataa gcagatgctg
     1741 ttatttacac acatacgact ggcccatggc ttttctaatc acaggaagcg attgcaggca
     1801 gttcaggcca gactgcatgc aatatctata ttagtgtatt ccaatgcctt gcaggaatca
     1861 gcaaacagta tcttgtataa tggcttgata gaggagttag tggacgtgct gcaaataacg
     1921 gataagcagc ttatggagat taaagcagct tctttacgaa cattaacatc aattgtccac
     1981 ttggagagaa ctcccaaact cagcagtatt attgactgta ctggaactgc ctcctaccat
     2041 ggatttttgc cagtgcttgt aaggaactgt atccaggcca tgattgatcc ttccatggat
     2101 ccataccctc accagtttgc cactgctctc ttctcttttt tataccatct ggccagctac
     2161 gatgctggtg gtgaagcctt ggtctcctgt ggaatgatgg aagccttatt gaaggtcata
     2221 aagtttcttg gcgatgaaca ggaccagata acatttgtca ccagagccgt cagagtggtt
     2281 gaccttatca ccaacctgga tatggcagct tttcaatccc atagtggact ttctatcttc
     2341 atttatagac ttgagcatga agtagatctg tgccgaaaag aatgtccgtt tgtgatcaag
     2401 ccaaagatcc agagacccaa tactacacaa gaaggagagg aaatggaaac tgatatggat
     2461 ggagtccagt gtattccaca acgagcagca cttctgaaat ccatgttgaa tttcctcaag
     2521 aaggccatcc aagaccctgc tttctcagat ggcatacgac atgtgatgga tggttctctg
     2581 cctacctccc tgaaacacat catcagcaat gcagaatact atggcccatc actcttcctc
     2641 ctagctactg aagtggtgac tgtgtttgta tttcaagaac catcactgct ctcctcactc
     2701 caggacaatg gattgacaga tgtcatgctg catgcactgc ttatcaaaga tgttcctgct
     2761 acccgtgaag tccttggctc cctcccaaat gtattcagtg cactctgttt gaatgcccga
     2821 ggtcttcagt cttttgttca gtgtcagcct tttgaacgcc tcttcaaagt tcttctgtct
     2881 ccagattacc tcccagccat gcggaggagg agaagttctg atccccttgg ggatactgca
     2941 tccaacctgg ggagtgctgt cgatgagctc atgagacatc agcccaccct taaaacagat
     3001 gcaacgactg ccatcatcaa gttacttgaa gaaatctgta atcttggaag ggaccccaaa
     3061 tacatctgtc agaagccatc gatccagaag gcagatggca ctgccactgc tcctccccca
     3121 aggtctaatc atgccgcaga agaagcctct agtgaggatg aggaggaaga ggaagtacag
     3181 gccatgcaga gctttaattc tacccagcaa aatgaaactg agcctaatca gcaggttgtt
     3241 ggtacagagg aacgtattcc tattcccctc atggattaca tccttaatgt gatgaaattt
     3301 gtggaatcta ttctgagcaa caatacaaca gatgaccact gccaggaatt tgtgaatcag
     3361 aaaggactgt tgcctttggt taccattttg ggtcttccca atctgcccat tgactttccc
     3421 acatctgctg cctgtcaggc tgttgcaggt gtctgcaaat ccatattgac actgtcacat
     3481 gaacccaaag tccttcaaga gggtctcctt cagttggact ccatcctctc ctccctggag
     3541 cccttacacc gccccattga atcccctggg ggctcagtgt tgttgcgaga actggcttgc
     3601 gcaggcaatg ttgctgatgc taccctctca gcccaggcca cacctctgct gcatgcactc
     3661 actgctgccc atgcctacat catgatgttt gttcatactt gcagagttgg acagagtgaa
     3721 attcgttcca tctccgtaaa ccagtggggc tctcaattgg gtctgagtgt tttgagcaag
     3781 ctgagccagt tatactgttc cctggtgtgg gaaagcactg tcctcctctc tctgtgtacc
     3841 ccaaacagcc taccatctgg gtgtgaattt ggccaggcag atatgcagaa actggttcca
     3901 aaggatgaga aggcaggtac gacccagggc ggaaaaagat cagatgggga acaggatgga
     3961 gcagctggaa gtatggatgc ttctacccag ggcttattag aaggcattgg gctagatggt
     4021 gacacattgg ctcccatgga gacagatgaa cctactgctt cagactctaa gggcaaatct
     4081 aaaatcacac cagcaatggc tgccagaatt aagcaaatca agcctttgtt atcagcttcc
     4141 tccagattag gccgagcact tgctgagcta tttggacttc ttgttaaact ttgtgtggga
     4201 tctcctgtcc gccagagaag gagccatcat gctgccagca ccactacagc accgacacct
     4261 gccgcgcgat caacagcctc agctctcact aagctcttga ctaaggggtt atcttggcag
     4321 cccccaccat atacacctac tccccgattc aggctgacat tcttcatctg ttcagttggt
     4381 ttcacatccc caatgctgtt tgatgagagg aagtatccct accacctcat gctgcaaaaa
     4441 tttctctgct ccggaggcca caatgctctt tttgaaactt tcaactgggc tctgtccatg
     4501 ggaggtaaag ttcctgtttc tgagggattg gaacactcag acttgcctga tggcacagga
     4561 gaattcctag atgcctggct tatgctggtg gagaagatgg tgaatcccac cacggtgctt
     4621 gaatctccac attcgctgcc tgccaaattg cctggaggtg tccagaactt tccccagttc
     4681 agtgcactgc gcttccttgt ggtaactcag aaagcagcct ttacttgcat caaaaactta
     4741 tggaaccgga aacccctgaa ggtatatggt ggacgaatgg ctgaatcgat gctggccatt
     4801 ctatgccaca tcctccgagg agaacctgtg attcgagaga gactaagcaa ggagaaggag
     4861 gggtctcgag gagaagagga tacagggcaa gaggaaggtg gctcccgccg ggaacctcaa
     4921 gtcaaccagc aacaactgca acagctcatg gacatgggct tcacaaggga acatgcaatg
     4981 gaggcactgt tgaacaccag caccatggag caggccacag agtacctttt aacccaccct
     5041 cctccaatca tgggaggagt tgttcgggat ctcagcatgt ctgaagagga ccagatgatg
     5101 agagcaattg ctatgtctct gggacaggat attccaatgg atcaaagggc agagtcacct
     5161 gaggaagttg cttgccggaa ggaggaagag gaacggaaag ctcgggaaaa gcaggaggag
     5221 gaagaggcta aatgtctaga gaagttccag gatgctgacc cgttggaaca agatgagctc
     5281 cacactttca cagatactat gttgccaggc tgcttccacc ttcttgatga gctgccagac
     5341 acagtatacc gtgtgtgtga cctgatcatg acagcaatca aacgtaatgg agcagattat
     5401 cgtgacatga ttctgaagca agtagtcaat caggtgtggg aagctgctga tgtattgatc
     5461 aaagctgctc ttcccctgac aacaagtgac acaaaaaccg tgtcagagtg gataagtcag
     5521 atggccacac tgccccaggc ctccaatttg gctactagaa tcttgctttt aacgctactt
     5581 tttgaggagt tgaagctacc ttgtgcttgg gtggttgaat caagtggcat ccttaatgtc
     5641 ctaatcaaac tcttggaagt ggttcagccc tgcctccagg cagccaagga gcagaaggaa
     5701 gtccagaccc caaagtggat cacaccagtg ttgctcctga ttgatttcta tgaaaagaca
     5761 gccatctcct caaaaaggag agcccagatg actaagtacc tgcaatccaa cagcaacaac
     5821 tggcgctggt ttgatgatcg ctctgggcgt tggtgtagtt acagtgcaag caacaatagc
     5881 actattgatt ctgcctggaa atctggagag acaagcgtgc gattcactgc aggccgaaga
     5941 agatacacgg tccaattcac tacaatggtg caggttaatg aggaaacagg gaaccgacgc
     6001 cctgtgatgc tgactctcct cagggtacct cggctgaata aaaattcaaa aaacagcaat
     6061 ggacaggaac tagagaagac gctggaagaa agcaaagaaa tggatatcaa acgtaaagaa
     6121 aataaaggca atgatacccc tttggcccta gagagtacaa acactgaaaa ggagacaagc
     6181 ctggaggaaa caaaaatcgg ggagatcctg atccagggct tgacagaaga tatggtgact
     6241 gttttaatcc gggcctgcgt gagcatgctg ggagtccctg tggacccaga tactttgcat
     6301 gccacccttc gtctctgtct gaggctcacc cgggaccaca aatatgccat gatgtttgca
     6361 gaactgaaga gtacccgcat gatcttgaat ttgacccaga gctcaggctt caatgggttt
     6421 actcccctgg tcacccttct cttaagacac atcattgagg acccctgtac ccttcgtcat
     6481 accatggaaa aggttgttcg ctcagcagct acaagtggag ctggtagcac tacctctggt
     6541 gttgtgtctg gcagcctcgg ctctcgggag atcaactaca tccttcgtgt ccttgggcca
     6601 gccgcatgcc gcaatccaga catattcaca gaagtggcca actgctgtat ccgcatcgcc
     6661 cttcctgccc ctcgaggctc aggaactgct tcagatgatg aatttgagaa tcttagaatt
     6721 aaaggcccta atgctgtaca gctggtgaag accacccctt tgaagccctc acctctgcct
     6781 gtcatccctg atactatcaa ggaagtgatc tatgatatgc tgaatgctct ggctgcatac
     6841 catgctccag aggaagccga taaatctgat cctaaacctg gggttatgac ccaagaggtt
     6901 ggccagctcc tgcaagacat gggtgatgat gtataccagc agtaccggtc acttacgcgt
     6961 cagagcagtg actttgatac gcagtcaggt ttttccatta atagtcaggt ctttgctgca
     7021 gatggtgcct ccactgagac ttccgcatct gggacctccc aaggagaggc ttcaactcca
     7081 gaggagtctc gagatgggaa gaaagataaa gaaggggacc gggcctctga ggaaggcaaa
     7141 cagaaaggca agggcagcaa acctttaatg cctacctcca ctatccttcg tcttctggca
     7201 gagttggtga ggtcctatgt tggtattgct accctgattg ccaactacag ctacactgtg
     7261 ggccagtctg aactgatcaa agaggactgc agtgtgctag cttttgttct ggaccacctg
     7321 ctcccacata cccagaatgc agaagacaag gacacccctg ccttggcccg cctgttcctc
     7381 gcaagcctgg ctgctgcagg gagtggcaca gatgcccagg tggccctagt gaatgaagta
     7441 aaagcagccc ttggacgggc actggctatg gctgagagta cagagaaaca tgccaggctt
     7501 caggcagtga tgtgtatcat cagtactatc atggagtcct gcccctccac ctccagcttc
     7561 tacagcagtg ccacagcgaa gacccagcac aatggcatga acaacatcat tcggcttttc
     7621 ctgaagaagg gactggttaa tgacctggcc agagtacctc acagcttaga cctgtccagt
     7681 cccaacatgg ccaacacagt caatgctgct ctgaagcctt tggaaacact ttcccggatt
     7741 gtgaaccagc ccagtagcct ttttggcagc aagagtgctt ctagcaagaa caagtctgag
     7801 caggatgccc aaggagcctc tcaagattcc agtagcaacc agcaggaccc aggcgagcct
     7861 ggggaagcag aagtgcagga ggaggatcat gatgtcactc agacagaggt ggcagatggg
     7921 gatatcatgg atggggaggc tgaaaccgac tcagtggtga ttgctgggca gcctgaggtg
     7981 ctcagttcac aagagatgca ggttgagaat gagctggagg acctgataga tgagttgctt
     8041 gagagggatg gcggatctgg gaacagtaca attatagtga gcagaagtgg agaggatgaa
     8101 tcacaagagg acgtgctgat ggatgaagct ccttccaacc tcagccaagc ttccaccttg
     8161 caggccaacc gagaagattc catgaatatc ctggaccctg aggatgagga ggagcacact
     8221 caggaagagg acagcagtgg cagtaacgag gatgaggatg atagtcagga tgaagaggag
     8281 gaggaggagg aagatgagga agatgatcag gaggatgatg aaggtgaaga gggagatgaa
     8341 gacgatgacg acgatggctc tgagatggaa ttggatgagg attatcctga tatgaacgct
     8401 tctcccttgg tccgatttga gcgctttgac cgggaggatg atctcatcat tgagtttgac
     8461 aacatgttct ccagtgctac agacatcccc ccatccccag gaaatatccc taccacccat
     8521 ccactgatgg tgcgccatgc agaccacagt tctctgacac tgggcagtgg ctcttcaaca
     8581 actcgtctca cccagggcat cgggcgcagt cagaggaccc taaggcagct gacggccaat
     8641 actggccaca ccattcatgt tcactaccct gggaatcgcc agcccaaccc tcctcttata
     8701 ctgcagaggt tgcttggtcc ctcagctgct gctgacatcc ttcagctgag cagcagcctt
     8761 cccctacaaa gccggggtcg ggcccgcctc ctggtaggca acgatgacgt ccacatcatc
     8821 gcccgttctg atgatgagct gctggatgac tttttccatg atcagagcac agctaccagc
     8881 caagcaggaa ccctgtccag catccccaca gccctgaccc gctggacaga agaatgcaaa
     8941 gttctcgatg ctgagagcat gcatgactgt gtttcagtgg ttaaagtgtc cattgtcaat
     9001 cacctggaat tcctgaggga tgaggagctg gaagaaaggc gagagaagcg caggaaacaa
     9061 ctggctgagg aagaaacaaa gataactgat aaaggcaaag aagataagga gaacagggat
     9121 cagagtgcac agtgtactgc atctaagtca aatgactcca ctgaacagaa tctctcagat
     9181 gggacgccta tgcctgacag ctacccaaca accccatctt caactgatgc agctacatct
     9241 gagtccaagg agacccttgg cactctgcaa tcctcacaac agcaaccaac actcccaacc
     9301 ccaccagctt tgggagaggt tcctcaggag ctgcagtctc cagctggaga agggggcagc
     9361 tctacacagc tattgatgcc tgtagagcca gaggaattgg gtcccacaag gccaagtggg
     9421 gaagcagaaa caactcagat ggagttatcc ccagctccca ctataacctc actttcccca
     9481 gagagagctg aggattctga tgcactgacg gctgtcagca gtcagctaga aggctctcct
     9541 atggatacaa gcagcctggc ttcctgtacc ttagaggagg ctgtgggtga cacttcagca
     9601 gctggcagtt ctgagcagcc cagagcaggc agctccactc ctggggatgc cccaccagct
     9661 gtggcggaag tgcaaggcag gagtgatggg tcaggggaat ctgcccagcc acctgaggac
     9721 agctccccac ctgcatcctc tgagagctct tccaccagag attctgccgt ggccatttct
     9781 ggcgccgatt cccgaggaat cctagaagag ccgttgcctt caacaagcag tgaagaagaa
     9841 gatccccttg cgggtatcag tctccctgaa ggtgtggacc cctcttttct ggctgccctg
     9901 cctgatgaca tccgtcggga agttctacag aaccagctag gcattcgtcc accaacccgg
     9961 actgccccct ccacaaatag ctcagcgcct gcagtggtgg ggaatcctgg tgtgactgag
    10021 gtgagtcccg agtttcttgc ggcactgcca cccgcgatcc aagaggaagt actggcacag
    10081 cagagagctg agcagcagcg acgagaacta gcacagaatg ccagctcaga cacccctatg
    10141 gaccctgtga ccttcatcca gactctgccc tcagacctgc gccgtagtgt cctagaggat
    10201 atggaggaca gtgtgttagc tgtgatgcca cctgacattg cagctgaggc tcaagccctg
    10261 agacgagagc aagaagcccg gcagcgacag ctcatgcatg agcgtctgtt tgggcacagt
    10321 agcacctccg cactctctgc tattctccga agcccggctt tcaccagtcg cttaagtggc
    10381 aaccgtgggg tccagtatac tcgccttgct gtgcagagag gtggcacctt ccagatgggg
    10441 ggtagcagca gccataacag gccttctggc agtaatgtag atactctcct ccgcctccga
    10501 ggacggctcc ttctggacca cgaagccctt tcttgtctct tggtcctact ttttgtggat
    10561 gagccaaagc tcaatactag ccgtctacac cgagtactga gaaatctctg ctaccatgcc
    10621 cagacccgcc actgggtcat ccgcagtctg ctctccatct tgcagcgcag cagtgagagt
    10681 gagctatgca ttgaaacacc caaactcact acaagtgagg aaaagggcaa aaagtcgagc
    10741 aagagctgtg ggtcaagtag ccatgagaac cgtcccctgg acctgctaca caagatggag
    10801 tcaaagagct ccaaccagct ttcctggctc tcagtatcca tggatgcagc cctaggctgc
    10861 aggactaata tatttcagat ccagcgttca ggggggcgta aacataccga gaagcatgca
    10921 agcggtggct ccaccgtcca catccatccc caagctgctc ctgttgtctg cagacacgtt
    10981 ttggatacac tcattcaatt ggccaaggta tttcccagcc acttcacaca gcagcggacc
    11041 aaagaaacaa actgtgagag tgatcgggaa aggggcaata aggcctgtag cccatgctcc
    11101 tcacagtcct ccagcagtgg catttgcaca gacttctggg acttattggt aaaactggac
    11161 aacatgaatg tcagccggaa aggcaagaac tccgtgaagt cagtgccagt gagcgctggc
    11221 ggtgaggggg aaacctctcc atacagcctc gaggcctctc cactggggca gctcatgaac
    11281 atgttgtcac acccagtcat ccgccggagc tctctcttaa ctgagaaact cctcagactc
    11341 ctttctctca tctcaattgc tctcccagaa aacaaggtgt cagaagcaca ggctaattct
    11401 ggcagcggtg cttcctccac caccactgcc acctcaacca catctaccac caccaccact
    11461 gccgcctcca ccacgcccac accccctact gcacccaccc ctgtcacttc tgctccagcc
    11521 ctggttgctg ccacggctat ttccaccatt gtcgtagctg cttcgaccac agtgactacc
    11581 cccacgactg ctaccactac tgtttcaatt tctcccacta ctaagggcag caaatctcca
    11641 gcgaaggtga gtgatggggg cagcagcagt acagacttta agatggtgtc ctctggcctc
    11701 actgaaaacc agctacagct ctctgtagag gtgttgacat cccactcttg ttctgaggaa
    11761 ggcttagagg atgcagccaa cgtactactg cagctctccc ggggggactc tgggacccgg
    11821 gacactgttc tcaagctgct actgaatgga gcccgccatc tgggttatac cctttgtaaa
    11881 caaataggta ccctgctggc cgagctgcgg gaatacaacc tcgagcagca gcggcgagcc
    11941 caatgtgaaa ccctctctcc tgatggcctg cctgaggagc agccacagac caccaagctg
    12001 aagggcaaaa tgcagagcag gtttgacatg gctgagaatg tggtaattgt ggcatctcag
    12061 aagcgacctt tgggtggccg ggagctccag ctgccttcta tgtccatgtt gacatccaag
    12121 acatctaccc agaagttctt cttgagggta ctacaggtca tcatccagct ccgggacgac
    12181 acgcgccggg ctaacaagaa agccaagcag acaggcaggc taggttcctc cggtttaggc
    12241 tcagctagca gcatccaggc agctgttcgg cagctggagg ctgaggctga tgccattata
    12301 caaatggtac gtgagggtca aagggcgcgg agacagcaac aagcagcaac gtcggagtct
    12361 agccagtcag aggcgtctgt ccggagggag gaatcaccca tggatgtgga ccagccatct
    12421 cccagtgctc aagatactca atccattgcc tccgatggaa ccccacaggg ggagaaggaa
    12481 aaggaagaaa gaccacctga gttacccctg ctcagcgagc agctgagttt ggacgagctg
    12541 tgggacatgc ttggggagtg tctaaaggaa ctagaggaat cccatgacca gcatgcggtg
    12601 ctagtgctac agcctgctgt cgaggccttc tttctggtcc atgccacaga gcgggagagc
    12661 aagcctcctg tccgagacac ccgtgagagc cagctggcac acatcaagga cgagcctcct
    12721 ccactctccc ctgccccctt aaccccagcc acgccttcct cccttgaccc attcttctcc
    12781 cgggagccct catctatgca catctcctca agcctgcccc ctgacacaca gaagttcctt
    12841 cgctttgcag agactcaccg cactgtgtta aaccagatcc tacggcagtc cacgacccac
    12901 cttgctgatg ggccttttgc tgtcctggta gactacattc gtgtcctcga ctttgatgtc
    12961 aagcgcaaat atttccgcca agagctggag cgtttagatg aggggctccg gaaagaagac
    13021 atggctgtgc atgtccgtcg tgaccatgtg tttgaagact cctatcgtga gctgcatcgc
    13081 aaatcccccg aagaaatgaa gaatcgattg tatatagtat ttgaaggaga agaagggcag
    13141 gatgctggcg ggctcctgcg ggagtggtat atgatcatct ctcgagagat gtttaaccct
    13201 atgtatgcct tgttccgtac ctcacctggt gatcgagtca cctacaccat caatccatct
    13261 tcccactgca accccaacca tctgtcctat tttaaatttg tcggacgcat tgtggccaaa
    13321 gctgtatatg acaaccgtct tctggagtgc tactttactc gatcctttta caaacacatc
    13381 ttgggcaagt cagtcagata tacagatatg gagagtgaag attaccactt ctaccaaggt
    13441 ctggtttatc tgctggaaaa tgatgtctcc acactaggct atgacctcac cttcagcact
    13501 gaggtccaag agtttggagt ttgtgaagtt cgtgacctca aacccaatgg ggccaacatc
    13561 ttggtaacag aggagaataa gaaggagtat gtacacctgg tatgccagat gagaatgaca
    13621 ggagccatcc gcaagcagtt ggcggctttc ttagaaggct tctatgagat cattccaaag
    13681 cgcctcattt ccatcttcac tgagcaggag ttagagctgc ttatatcagg actgcccacc
    13741 attgacatcg atgatctgaa atccaacact gaataccaca agtaccagtc caactctatt
    13801 cagatccagt ggttctggag agcattgcgt tctttcgatc aagctgaccg tgccaagttc
    13861 ctccagtttg tcacgggtac ttccaaggta cccctgcaag gctttgctgc cctcgaaggc
    13921 atgaatggca ttcagaagtt tcagatccat cgagatgaca ggtccacaga tcgcctgcct
    13981 tcagctcaca catgttttaa tcagctggat ctgcctgcct atgagagctt tgagaagctc
    14041 cgccacatgc tactgttggc tatccaggag tgctctgaag gctttgggct ggcctaataa
    14101 gcggccgcga cccagctttc ttgtacaaag tggttgatat cggtaccagt cgactctaga
    14161 ggatcccggg tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc
    14221 cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta
    14281 ggtgtccttc tataatatta tggggtggag ggggggtggt atggagcaag gggcaagttg
    14341 ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagggcaca
    14401 atcttggctc actgcaatct ccgctctggg ttcaagcgat tctcctgcct cacctccgag
    14461 ttgtgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac
    14521 ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg atctacccac
    14581 cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc
    14641 tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg ctacctgcca
    14701 tggcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca tgcgttgggt
    14761 ccactcagta gatgcctgtt gaattgggta cgcggccagc ttctgtggaa tgtgtgtcag
    14821 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
    14881 aattagtcag caaccaggtg tggaaaagtc cccaggctcc ccagcaggca gaagtatgca
    14941 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
    15001 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    15061 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
    15121 tggaggccta ggcttttgca aaaagctcct cgaggaactg aaaaaccaga aagttaattc
    15181 cctatagtga gtcgtattaa attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt
    15241 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
    15301 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
    15361 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
    15421 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    15481 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    15541 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    15601 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    15661 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    15721 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    15781 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
    15841 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    15901 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    15961 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    16021 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
    16081 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    16141 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    16201 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    16261 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    16321 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    16381 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    16441 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    16501 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    16561 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    16621 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    16681 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    16741 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    16801 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    16861 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    16921 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    16981 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    17041 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    17101 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    17161 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    17221 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    17281 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    17341 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
    17401 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
    17461 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    17521 ggcatccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    17581 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    17641 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    17701 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
    17761 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaaa cgtttacaat
    17821 tt
//
