LOCUS       Exported               17885 bp DNA     circular SYN 01-JUN-2022
DEFINITION  Isoform1, BH3-4E (V1976E/V1780E/L1983E/M1987E), Expression clone.
ACCESSION   urn.local.mhunkeler.a1-evuri0k
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 7, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..17885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     143..159
                     /label=M13 fwd
                     /note="<html><body>common sequencing primer, one of
                     multiple similar variants</body></html>"
     enhancer        312..690
                     /label=CMV enhancer
                     /note="<html><body>human cytomegalovirus immediate early 
                     enhancer</body></html>"
     promoter        691..894
                     /label=CMV promoter
                     /note="<html><body>human cytomegalovirus (CMV) immediate
                     early promoter</body></html>"
     CDS             928..951
                     /codon_start=1
                     /product="<html><body>FLAG(R) epitope tag, followed by an 
                     enterokinase cleavage site</body></html>"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    989..1009
                     /label=attB1
                     /label=nonstandard type: attB1
                     /note="/annotation_group=attB"
     CDS             1036..14160
                     /codon_start=1
                     /label=Huwe1_cds
                     /note="/Original_Translation=MKVDRTKLKKTPTEAPADCRALIDKLKVCN
                     DEQLL 
                     LELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLL 
                     LAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRL 
                     GSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAE 
                     VKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGF 
                     SNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKA 
                     ASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFA 
                     TALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITN 
                     LDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDGVQ 
                     CIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLL 
                     ATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNA 
                     RGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTL 
                     KTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEE 
                     EEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDH 
                     CQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQ 
                     LDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMM 
                     FVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSG 
                     CEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAP 
                     METDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPV 
                     RQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGF 
                     TSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGT 
                     GEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCI 
                     KNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGS 
                     RREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSM 
                     SEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQD 
                     ADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVV 
                     NQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLP 
                     CAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSK 
                     RRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYT 
                     VQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKEN 
                     KGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTL 
                     HATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCT 
                     LRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANC 
                     CIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDM 
                     LNALAAYHAPEEDKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFS 
                     INSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLM 
                     PTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAE 
                     DKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCI 
                     ISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMA 
                     NTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGE 
                     AEVQEEDHDVTHTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELL 
                     ERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEE 
                     HTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYP 
                     DMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTL 
                     GSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAAD 
                     ILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPT 
                     ALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKI 
                     TDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETL 
                     GTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAET 
                     TQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAG 
                     SSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAIS 
                     GADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPP 
                     TRTAPSTNSSAPAVVGNPGVTEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSD 
                     LRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILR 
                     SPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHE 
                     ALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIET 
                     PKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNI 
                     FQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKE 
                     TNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAG 
                     GEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQA 
                     NSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAAST 
                     TVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTS 
                     HSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREY 
                     NLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQ 
                     LPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAA 
                     VRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDT 
                     QSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQ 
                     PAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSRE 
                     PSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDV 
                     KRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEE 
                     GQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGR 
                     IVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGY 
                     DLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLE 
                     GFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALR 
                     SFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQ 
                     LDLPAYESFEKLRHMLLLAIQECSEGFGLA*"
                     /translation="MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWN
                     IGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLI
                     EYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRL
                     QHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLH
                     YIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARL
                     HAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERT
                     PKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAG
                     GEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIY
                     RLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKK
                     AIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSL
                     QDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLL
                     SPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRD
                     PKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQ
                     QVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLP
                     IDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLL
                     RELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLG
                     LSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKR
                     SDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQ
                     IKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTK
                     LLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNAL
                     FETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAK
                     LPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGE
                     PVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTS
                     TMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACR
                     KEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRV
                     CDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATL
                     PQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQT
                     PKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTI
                     DSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNG
                     QELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVT
                     VLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNG
                     FTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVL
                     GPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPS
                     PLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEEGQLEQDEGDDEYQQYR
                     SLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRA
                     SEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLA
                     FVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAES
                     TEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARV
                     PHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSS
                     SNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVEN
                     ELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMN
                     ILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSE
                     MELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHA
                     DHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLG
                     PSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTL
                     SSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEE
                     ETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESK
                     ETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEA
                     ETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAA
                     GSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAIS
                     GADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPT
                     RTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDT
                     PMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLF
                     GHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTL
                     LRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQ
                     RSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSM
                     DAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPS
                     HFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSV
                     KSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPEN
                     KVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTI
                     VVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSV
                     EVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAE
                     LREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGR
                     ELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQ
                     AAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQD
                     TQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQ
                     PAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREP
                     SSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKR
                     KYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQD
                     AGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAK
                     AVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFS
                     TEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEII
                     PKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADR
                     AKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYES
                     FEKLRHMLLLAIQECSEGFGLA"
     misc_feature    complement(14176..14196)
                     /direction=LEFT
                     /label=attB2
                     /label=nonstandard type: attB2
                     /note="/annotation_group=attB"
     promoter        14875..15205
                     /label=SV40 promoter
                     /note="<html><body>SV40 enhancer and early 
                     promoter</body></html>"
     rep_origin      15056..15191
                     /label=SV40 ori
                     /note="<html><body>SV40 origin of
                     replication</body></html>"
     promoter        complement(15244..15262)
                     /label=T7 promoter
                     /note="<html><body>promoter for bacteriophage T7 RNA 
                     polymerase</body></html>"
     primer_bind     complement(15276..15292)
                     /label=M13 rev
                     /note="<html><body>common sequencing primer, one of
                     multiple similar variants</body></html>"
     protein_bind    15300..15316
                     /label=lac operator
                     /bound_moiety="<html><body><i>lac</i> repressor encoded by 
                     <i>lacI</i></body></html>"
                     /note="<html><body>The <i>lac</i> repressor binds to the 
                     <i>lac</i> operator to inhibit transcription in <i>E. 
                     coli</i>. This inhibition can be relieved by adding lactose
                     or isopropyl-beta-D-thiogalactopyranoside 
                     (IPTG).</body></html>"
     promoter        complement(join(15349..15354,15331..15348,15324..15330))
                     /label=lac promoter
                     /note="<html><body>promoter for the <i>E. coli lac</i> 
                     operon</body></html>"
     protein_bind    15369..15390
                     /label=CAP binding site
                     /bound_moiety="<html><body><i>E. coli</i> catabolite
                     activator protein</body></html>"
                     /note="<html><body>CAP binding activates transcription in
                     the presence of cAMP.</body></html>"
     rep_origin      complement(15678..16266)
                     /direction=LEFT
                     /label=ori
                     /note="<html><body>high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication</body></html>"
     CDS             complement(join(17229..17297,16437..17228))
                     /codon_start=1
                     /gene="<html><body><i>bla</i></body></html>"
                     /product="<html><body>beta-lactamase</body></html>"
                     /label=AmpR
                     /note="<html><body>confers resistance to ampicillin, 
                     carbenicillin, and related antibiotics</body></html>"
                     /translation="MPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMS
                     TFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITM
                     SDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMAT
                     TLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIA
                     ALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*MSIQHFRVALIPFFAAF
                     CLPVFA"
     promoter        complement(17298..17402)
                     /gene="<html><body><i>bla</i></body></html>"
                     /label=AmpR promoter
     rep_origin      17429..17885
                     /direction=RIGHT
                     /label=f1 ori
                     /note="<html><body>f1 bacteriophage origin of replication;
                     arrow indicates direction of (+) strand 
                     synthesis</body></html>"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccca
      121 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctgat ctaatcaata
      181 ttggccatta gccatattat tcattggtta tatagcataa atcaatattg gctattggcc
      241 attgcatacg ttgtatccat atcataatat gtacatttat attggctcat gtccaacatt
      301 accgccatgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      361 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      421 cgaccgccca gcgacccccg cccgttgacg tcaatagtga cgtatgttcc catagtaacg
      481 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      541 gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa
      601 tggcccgcct agcattatgc ccagtacatg accttacggg agtttcctac ttggcagtac
      661 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg
      721 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      781 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg
      841 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      901 gtgaaccgtc agaattgatc taccatggac tacaaagacg atgacgacaa gcttgcggcc
      961 gcgaattcat cgatagatct gatatcaaca agtttgtaca aaaaagcagg ctttaaagga
     1021 accaattcag tcgacatgaa agtagacagg actaaactga agaagacacc tactgaggct
     1081 cctgcagact gcagagcctt aatagacaaa ctcaaagttt gtaatgatga gcaacttctc
     1141 ttggaactgc agcagatcaa aacatggaac attggaaagt gcgagttata tcactgggtg
     1201 gacctgttgg accgcttcga tggaatactg gcagatgctg gacagacagt ggagaatatg
     1261 tcatggatgc tcgtatgtga taggccagaa agagagcaac tgaaaatgct tctcttggct
     1321 gtgttgaact tcacagcctt gctcattgag tacagctttt cccggcatct gtacagttcc
     1381 atagagcatt tgacaacttt attggcttcc tctgatatgc aagtggtgct ggcagtcctc
     1441 aatctcctat atgtatttag caaaagatca aactacatca ctcgtctggg atctgacaag
     1501 aggaccccgc tgctaactcg gctacaacat ttggcagaga gctggggtgg aaaggagaat
     1561 ggctttggac ttgcagaatg ttgcagagac ttgcatatga tgaaatatcc acccagtgca
     1621 actacactac actttgaatt ctatgcagat cctggggccg aggtcaaaat tgagaaaagg
     1681 acaactagta acacactaca ttatattcac atagagcaac ttgacaagat ttcagaaagc
     1741 ccttctgaaa tcatggaatc tcttaccaaa atgtacagca ttcctaagga taagcagatg
     1801 ctgttattta cacacatacg actggcccat ggcttttcta atcacaggaa gcgattgcag
     1861 gcagttcagg ccagactgca tgcaatatct atattagtgt attccaatgc cttgcaggaa
     1921 tcagcaaaca gtatcttgta taatggcttg atagaggagt tagtggacgt gctgcaaata
     1981 acggataagc agcttatgga gattaaagca gcttctttac gaacattaac atcaattgtc
     2041 cacttggaga gaactcccaa actcagcagt attattgact gtactggaac tgcctcctac
     2101 catggatttt tgccagtgct tgtaaggaac tgtatccagg ccatgattga tccttccatg
     2161 gatccatacc ctcaccagtt tgccactgct ctcttctctt ttttatacca tctggccagc
     2221 tacgatgctg gtggtgaagc cttggtctcc tgtggaatga tggaagcctt attgaaggtc
     2281 ataaagtttc ttggcgatga acaggaccag ataacatttg tcaccagagc cgtcagagtg
     2341 gttgacctta tcaccaacct ggatatggca gcttttcaat cccatagtgg actttctatc
     2401 ttcatttata gacttgagca tgaagtagat ctgtgccgaa aagaatgtcc gtttgtgatc
     2461 aagccaaaga tccagagacc caatactaca caagaaggag aggaaatgga aactgatatg
     2521 gatggagtcc agtgtattcc acaacgagca gcacttctga aatccatgtt gaatttcctc
     2581 aagaaggcca tccaagaccc tgctttctca gatggcatac gacatgtgat ggatggttct
     2641 ctgcctacct ccctgaaaca catcatcagc aatgcagaat actatggccc atcactcttc
     2701 ctcctagcta ctgaagtggt gactgtgttt gtatttcaag aaccatcact gctctcctca
     2761 ctccaggaca atggattgac agatgtcatg ctgcatgcac tgcttatcaa agatgttcct
     2821 gctacccgtg aagtccttgg ctccctccca aatgtattca gtgcactctg tttgaatgcc
     2881 cgaggtcttc agtcttttgt tcagtgtcag ccttttgaac gcctcttcaa agttcttctg
     2941 tctccagatt acctcccagc catgcggagg aggagaagtt ctgatcccct tggggatact
     3001 gcatccaacc tggggagtgc tgtcgatgag ctcatgagac atcagcccac ccttaaaaca
     3061 gatgcaacga ctgccatcat caagttactt gaagaaatct gtaatcttgg aagggacccc
     3121 aaatacatct gtcagaagcc atcgatccag aaggcagatg gcactgccac tgctcctccc
     3181 ccaaggtcta atcatgccgc agaagaagcc tctagtgagg atgaggagga agaggaagta
     3241 caggccatgc agagctttaa ttctacccag caaaatgaaa ctgagcctaa tcagcaggtt
     3301 gttggtacag aggaacgtat tcctattccc ctcatggatt acatccttaa tgtgatgaaa
     3361 tttgtggaat ctattctgag caacaataca acagatgacc actgccagga atttgtgaat
     3421 cagaaaggac tgttgccttt ggttaccatt ttgggtcttc ccaatctgcc cattgacttt
     3481 cccacatctg ctgcctgtca ggctgttgca ggtgtctgca aatccatatt gacactgtca
     3541 catgaaccca aagtccttca agagggtctc cttcagttgg actccatcct ctcctccctg
     3601 gagcccttac accgccccat tgaatcccct gggggctcag tgttgttgcg agaactggct
     3661 tgcgcaggca atgttgctga tgctaccctc tcagcccagg ccacacctct gctgcatgca
     3721 ctcactgctg cccatgccta catcatgatg tttgttcata cttgcagagt tggacagagt
     3781 gaaattcgtt ccatctccgt aaaccagtgg ggctctcaat tgggtctgag tgttttgagc
     3841 aagctgagcc agttatactg ttccctggtg tgggaaagca ctgtcctcct ctctctgtgt
     3901 accccaaaca gcctaccatc tgggtgtgaa tttggccagg cagatatgca gaaactggtt
     3961 ccaaaggatg agaaggcagg tacgacccag ggcggaaaaa gatcagatgg ggaacaggat
     4021 ggagcagctg gaagtatgga tgcttctacc cagggcttat tagaaggcat tgggctagat
     4081 ggtgacacat tggctcccat ggagacagat gaacctactg cttcagactc taagggcaaa
     4141 tctaaaatca caccagcaat ggctgccaga attaagcaaa tcaagccttt gttatcagct
     4201 tcctccagat taggccgagc acttgctgag ctatttggac ttcttgttaa actttgtgtg
     4261 ggatctcctg tccgccagag aaggagccat catgctgcca gcaccactac agcaccgaca
     4321 cctgccgcgc gatcaacagc ctcagctctc actaagctct tgactaaggg gttatcttgg
     4381 cagcccccac catatacacc tactccccga ttcaggctga cattcttcat ctgttcagtt
     4441 ggtttcacat ccccaatgct gtttgatgag aggaagtatc cctaccacct catgctgcaa
     4501 aaatttctct gctccggagg ccacaatgct ctttttgaaa ctttcaactg ggctctgtcc
     4561 atgggaggta aagttcctgt ttctgaggga ttggaacact cagacttgcc tgatggcaca
     4621 ggagaattcc tagatgcctg gcttatgctg gtggagaaga tggtgaatcc caccacggtg
     4681 cttgaatctc cacattcgct gcctgccaaa ttgcctggag gtgtccagaa ctttccccag
     4741 ttcagtgcac tgcgcttcct tgtggtaact cagaaagcag cctttacttg catcaaaaac
     4801 ttatggaacc ggaaacccct gaaggtatat ggtggacgaa tggctgaatc gatgctggcc
     4861 attctatgcc acatcctccg aggagaacct gtgattcgag agagactaag caaggagaag
     4921 gaggggtctc gaggagaaga ggatacaggg caagaggaag gtggctcccg ccgggaacct
     4981 caagtcaacc agcaacaact gcaacagctc atggacatgg gcttcacaag ggaacatgca
     5041 atggaggcac tgttgaacac cagcaccatg gagcaggcca cagagtacct tttaacccac
     5101 cctcctccaa tcatgggagg agttgttcgg gatctcagca tgtctgaaga ggaccagatg
     5161 atgagagcaa ttgctatgtc tctgggacag gatattccaa tggatcaaag ggcagagtca
     5221 cctgaggaag ttgcttgccg gaaggaggaa gaggaacgga aagctcggga aaagcaggag
     5281 gaggaagagg ctaaatgtct agagaagttc caggatgctg acccgttgga acaagatgag
     5341 ctccacactt tcacagatac tatgttgcca ggctgcttcc accttcttga tgagctgcca
     5401 gacacagtat accgtgtgtg tgacctgatc atgacagcaa tcaaacgtaa tggagcagat
     5461 tatcgtgaca tgattctgaa gcaagtagtc aatcaggtgt gggaagctgc tgatgtattg
     5521 atcaaagctg ctcttcccct gacaacaagt gacacaaaaa ccgtgtcaga gtggataagt
     5581 cagatggcca cactgcccca ggcctccaat ttggctacta gaatcttgct tttaacgcta
     5641 ctttttgagg agttgaagct accttgtgct tgggtggttg aatcaagtgg catccttaat
     5701 gtcctaatca aactcttgga agtggttcag ccctgcctcc aggcagccaa ggagcagaag
     5761 gaagtccaga ccccaaagtg gatcacacca gtgttgctcc tgattgattt ctatgaaaag
     5821 acagccatct cctcaaaaag gagagcccag atgactaagt acctgcaatc caacagcaac
     5881 aactggcgct ggtttgatga tcgctctggg cgttggtgta gttacagtgc aagcaacaat
     5941 agcactattg attctgcctg gaaatctgga gagacaagcg tgcgattcac tgcaggccga
     6001 agaagataca cggtccaatt cactacaatg gtgcaggtta atgaggaaac agggaaccga
     6061 cgccctgtga tgctgactct cctcagggta cctcggctga ataaaaattc aaaaaacagc
     6121 aatggacagg aactagagaa gacgctggaa gaaagcaaag aaatggatat caaacgtaaa
     6181 gaaaataaag gcaatgatac ccctttggcc ctagagagta caaacactga aaaggagaca
     6241 agcctggagg aaacaaaaat cggggagatc ctgatccagg gcttgacaga agatatggtg
     6301 actgttttaa tccgggcctg cgtgagcatg ctgggagtcc ctgtggaccc agatactttg
     6361 catgccaccc ttcgtctctg tctgaggctc acccgggacc acaaatatgc catgatgttt
     6421 gcagaactga agagtacccg catgatcttg aatttgaccc agagctcagg cttcaatggg
     6481 tttactcccc tggtcaccct tctcttaaga cacatcattg aggacccctg tacccttcgt
     6541 cataccatgg aaaaggttgt tcgctcagca gctacaagtg gagctggtag cactacctct
     6601 ggtgttgtgt ctggcagcct cggctctcgg gagatcaact acatccttcg tgtccttggg
     6661 ccagccgcat gccgcaatcc agacatattc acagaagtgg ccaactgctg tatccgcatc
     6721 gcccttcctg cccctcgagg ctcaggaact gcttcagatg atgaatttga gaatcttaga
     6781 attaaaggcc ctaatgctgt acagctggtg aagaccaccc ctttgaagcc ctcacctctg
     6841 cctgtcatcc ctgatactat caaggaagtg atctatgata tgctgaatgc tctggctgca
     6901 taccatgctc cagaggaagc cgataaatct gatcctaaac ctggggttat gacccaagag
     6961 gaaggccagc tcgaacaaga cgaaggtgat gatgaatacc agcagtaccg gtcacttacg
     7021 cgtcagagca gtgactttga tacgcagtca ggtttttcca ttaatagtca ggtctttgct
     7081 gcagatggtg cctccactga gacttccgca tctgggacct cccaaggaga ggcttcaact
     7141 ccagaggagt ctcgagatgg gaagaaagat aaagaagggg accgggcctc tgaggaaggc
     7201 aaacagaaag gcaagggcag caaaccttta atgcctacct ccactatcct tcgtcttctg
     7261 gcagagttgg tgaggtccta tgttggtatt gctaccctga ttgccaacta cagctacact
     7321 gtgggccagt ctgaactgat caaagaggac tgcagtgtgc tagcttttgt tctggaccac
     7381 ctgctcccac atacccagaa tgcagaagac aaggacaccc ctgccttggc ccgcctgttc
     7441 ctcgcaagcc tggctgctgc agggagtggc acagatgccc aggtggccct agtgaatgaa
     7501 gtaaaagcag cccttggacg ggcactggct atggctgaga gtacagagaa acatgccagg
     7561 cttcaggcag tgatgtgtat catcagtact atcatggagt cctgcccctc cacctccagc
     7621 ttctacagca gtgccacagc gaagacccag cacaatggca tgaacaacat cattcggctt
     7681 ttcctgaaga agggactggt taatgacctg gccagagtac ctcacagctt agacctgtcc
     7741 agtcccaaca tggccaacac agtcaatgct gctctgaagc ctttggaaac actttcccgg
     7801 attgtgaacc agcccagtag cctttttggc agcaagagtg cttctagcaa gaacaagtct
     7861 gagcaggatg cccaaggagc ctctcaagat tccagtagca accagcagga cccaggcgag
     7921 cctggggaag cagaagtgca ggaggaggat catgatgtca ctcagacaga ggtggcagat
     7981 ggggatatca tggatgggga ggctgaaacc gactcagtgg tgattgctgg gcagcctgag
     8041 gtgctcagtt cacaagagat gcaggttgag aatgagctgg aggacctgat agatgagttg
     8101 cttgagaggg atggcggatc tgggaacagt acaattatag tgagcagaag tggagaggat
     8161 gaatcacaag aggacgtgct gatggatgaa gctccttcca acctcagcca agcttccacc
     8221 ttgcaggcca accgagaaga ttccatgaat atcctggacc ctgaggatga ggaggagcac
     8281 actcaggaag aggacagcag tggcagtaac gaggatgagg atgatagtca ggatgaagag
     8341 gaggaggagg aggaagatga ggaagatgat caggaggatg atgaaggtga agagggagat
     8401 gaagacgatg acgacgatgg ctctgagatg gaattggatg aggattatcc tgatatgaac
     8461 gcttctccct tggtccgatt tgagcgcttt gaccgggagg atgatctcat cattgagttt
     8521 gacaacatgt tctccagtgc tacagacatc cccccatccc caggaaatat ccctaccacc
     8581 catccactga tggtgcgcca tgcagaccac agttctctga cactgggcag tggctcttca
     8641 acaactcgtc tcacccaggg catcgggcgc agtcagagga ccctaaggca gctgacggcc
     8701 aatactggcc acaccattca tgttcactac cctgggaatc gccagcccaa ccctcctctt
     8761 atactgcaga ggttgcttgg tccctcagct gctgctgaca tccttcagct gagcagcagc
     8821 cttcccctac aaagccgggg tcgggcccgc ctcctggtag gcaacgatga cgtccacatc
     8881 atcgcccgtt ctgatgatga gctgctggat gactttttcc atgatcagag cacagctacc
     8941 agccaagcag gaaccctgtc cagcatcccc acagccctga cccgctggac agaagaatgc
     9001 aaagttctcg atgctgagag catgcatgac tgtgtttcag tggttaaagt gtccattgtc
     9061 aatcacctgg aattcctgag ggatgaggag ctggaagaaa ggcgagagaa gcgcaggaaa
     9121 caactggctg aggaagaaac aaagataact gataaaggca aagaagataa ggagaacagg
     9181 gatcagagtg cacagtgtac tgcatctaag tcaaatgact ccactgaaca gaatctctca
     9241 gatgggacgc ctatgcctga cagctaccca acaaccccat cttcaactga tgcagctaca
     9301 tctgagtcca aggagaccct tggcactctg caatcctcac aacagcaacc aacactccca
     9361 accccaccag ctttgggaga ggttcctcag gagctgcagt ctccagctgg agaagggggc
     9421 agctctacac agctattgat gcctgtagag ccagaggaat tgggtcccac aaggccaagt
     9481 ggggaagcag aaacaactca gatggagtta tccccagctc ccactataac ctcactttcc
     9541 ccagagagag ctgaggattc tgatgcactg acggctgtca gcagtcagct agaaggctct
     9601 cctatggata caagcagcct ggcttcctgt accttagagg aggctgtggg tgacacttca
     9661 gcagctggca gttctgagca gcccagagca ggcagctcca ctcctgggga tgccccacca
     9721 gctgtggcgg aagtgcaagg caggagtgat gggtcagggg aatctgccca gccacctgag
     9781 gacagctccc cacctgcatc ctctgagagc tcttccacca gagattctgc cgtggccatt
     9841 tctggcgccg attcccgagg aatcctagaa gagccgttgc cttcaacaag cagtgaagaa
     9901 gaagatcccc ttgcgggtat cagtctccct gaaggtgtgg acccctcttt tctggctgcc
     9961 ctgcctgatg acatccgtcg ggaagttcta cagaaccagc taggcattcg tccaccaacc
    10021 cggactgccc cctccacaaa tagctcagcg cctgcagtgg tggggaatcc tggtgtgact
    10081 gaggtgagtc ccgagtttct tgcggcactg ccacccgcga tccaagagga agtactggca
    10141 cagcagagag ctgagcagca gcgacgagaa ctagcacaga atgccagctc agacacccct
    10201 atggaccctg tgaccttcat ccagactctg ccctcagacc tgcgccgtag tgtcctagag
    10261 gatatggagg acagtgtgtt agctgtgatg ccacctgaca ttgcagctga ggctcaagcc
    10321 ctgagacgag agcaagaagc ccggcagcga cagctcatgc atgagcgtct gtttgggcac
    10381 agtagcacct ccgcactctc tgctattctc cgaagcccgg ctttcaccag tcgcttaagt
    10441 ggcaaccgtg gggtccagta tactcgcctt gctgtgcaga gaggtggcac cttccagatg
    10501 gggggtagca gcagccataa caggccttct ggcagtaatg tagatactct cctccgcctc
    10561 cgaggacggc tccttctgga ccacgaagcc ctttcttgtc tcttggtcct actttttgtg
    10621 gatgagccaa agctcaatac tagccgtcta caccgagtac tgagaaatct ctgctaccat
    10681 gcccagaccc gccactgggt catccgcagt ctgctctcca tcttgcagcg cagcagtgag
    10741 agtgagctat gcattgaaac acccaaactc actacaagtg aggaaaaggg caaaaagtcg
    10801 agcaagagct gtgggtcaag tagccatgag aaccgtcccc tggacctgct acacaagatg
    10861 gagtcaaaga gctccaacca gctttcctgg ctctcagtat ccatggatgc agccctaggc
    10921 tgcaggacta atatatttca gatccagcgt tcaggggggc gtaaacatac cgagaagcat
    10981 gcaagcggtg gctccaccgt ccacatccat ccccaagctg ctcctgttgt ctgcagacac
    11041 gttttggata cactcattca attggccaag gtatttccca gccacttcac acagcagcgg
    11101 accaaagaaa caaactgtga gagtgatcgg gaaaggggca ataaggcctg tagcccatgc
    11161 tcctcacagt cctccagcag tggcatttgc acagacttct gggacttatt ggtaaaactg
    11221 gacaacatga atgtcagccg gaaaggcaag aactccgtga agtcagtgcc agtgagcgct
    11281 ggcggtgagg gggaaacctc tccatacagc ctcgaggcct ctccactggg gcagctcatg
    11341 aacatgttgt cacacccagt catccgccgg agctctctct taactgagaa actcctcaga
    11401 ctcctttctc tcatctcaat tgctctccca gaaaacaagg tgtcagaagc acaggctaat
    11461 tctggcagcg gtgcttcctc caccaccact gccacctcaa ccacatctac caccaccacc
    11521 actgccgcct ccaccacgcc cacaccccct actgcaccca cccctgtcac ttctgctcca
    11581 gccctggttg ctgccacggc tatttccacc attgtcgtag ctgcttcgac cacagtgact
    11641 acccccacga ctgctaccac tactgtttca atttctccca ctactaaggg cagcaaatct
    11701 ccagcgaagg tgagtgatgg gggcagcagc agtacagact ttaagatggt gtcctctggc
    11761 ctcactgaaa accagctaca gctctctgta gaggtgttga catcccactc ttgttctgag
    11821 gaaggcttag aggatgcagc caacgtacta ctgcagctct cccgggggga ctctgggacc
    11881 cgggacactg ttctcaagct gctactgaat ggagcccgcc atctgggtta taccctttgt
    11941 aaacaaatag gtaccctgct ggccgagctg cgggaataca acctcgagca gcagcggcga
    12001 gcccaatgtg aaaccctctc tcctgatggc ctgcctgagg agcagccaca gaccaccaag
    12061 ctgaagggca aaatgcagag caggtttgac atggctgaga atgtggtaat tgtggcatct
    12121 cagaagcgac ctttgggtgg ccgggagctc cagctgcctt ctatgtccat gttgacatcc
    12181 aagacatcta cccagaagtt cttcttgagg gtactacagg tcatcatcca gctccgggac
    12241 gacacgcgcc gggctaacaa gaaagccaag cagacaggca ggctaggttc ctccggttta
    12301 ggctcagcta gcagcatcca ggcagctgtt cggcagctgg aggctgaggc tgatgccatt
    12361 atacaaatgg tacgtgaggg tcaaagggcg cggagacagc aacaagcagc aacgtcggag
    12421 tctagccagt cagaggcgtc tgtccggagg gaggaatcac ccatggatgt ggaccagcca
    12481 tctcccagtg ctcaagatac tcaatccatt gcctccgatg gaaccccaca gggggagaag
    12541 gaaaaggaag aaagaccacc tgagttaccc ctgctcagcg agcagctgag tttggacgag
    12601 ctgtgggaca tgcttgggga gtgtctaaag gaactagagg aatcccatga ccagcatgcg
    12661 gtgctagtgc tacagcctgc tgtcgaggcc ttctttctgg tccatgccac agagcgggag
    12721 agcaagcctc ctgtccgaga cacccgtgag agccagctgg cacacatcaa ggacgagcct
    12781 cctccactct cccctgcccc cttaacccca gccacgcctt cctcccttga cccattcttc
    12841 tcccgggagc cctcatctat gcacatctcc tcaagcctgc cccctgacac acagaagttc
    12901 cttcgctttg cagagactca ccgcactgtg ttaaaccaga tcctacggca gtccacgacc
    12961 caccttgctg atgggccttt tgctgtcctg gtagactaca ttcgtgtcct cgactttgat
    13021 gtcaagcgca aatatttccg ccaagagctg gagcgtttag atgaggggct ccggaaagaa
    13081 gacatggctg tgcatgtccg tcgtgaccat gtgtttgaag actcctatcg tgagctgcat
    13141 cgcaaatccc ccgaagaaat gaagaatcga ttgtatatag tatttgaagg agaagaaggg
    13201 caggatgctg gcgggctcct gcgggagtgg tatatgatca tctctcgaga gatgtttaac
    13261 cctatgtatg ccttgttccg tacctcacct ggtgatcgag tcacctacac catcaatcca
    13321 tcttcccact gcaaccccaa ccatctgtcc tattttaaat ttgtcggacg cattgtggcc
    13381 aaagctgtat atgacaaccg tcttctggag tgctacttta ctcgatcctt ttacaaacac
    13441 atcttgggca agtcagtcag atatacagat atggagagtg aagattacca cttctaccaa
    13501 ggtctggttt atctgctgga aaatgatgtc tccacactag gctatgacct caccttcagc
    13561 actgaggtcc aagagtttgg agtttgtgaa gttcgtgacc tcaaacccaa tggggccaac
    13621 atcttggtaa cagaggagaa taagaaggag tatgtacacc tggtatgcca gatgagaatg
    13681 acaggagcca tccgcaagca gttggcggct ttcttagaag gcttctatga gatcattcca
    13741 aagcgcctca tttccatctt cactgagcag gagttagagc tgcttatatc aggactgccc
    13801 accattgaca tcgatgatct gaaatccaac actgaatacc acaagtacca gtccaactct
    13861 attcagatcc agtggttctg gagagcattg cgttctttcg atcaagctga ccgtgccaag
    13921 ttcctccagt ttgtcacggg tacttccaag gtacccctgc aaggctttgc tgccctcgaa
    13981 ggcatgaatg gcattcagaa gtttcagatc catcgagatg acaggtccac agatcgcctg
    14041 ccttcagctc acacatgttt taatcagctg gatctgcctg cctatgagag ctttgagaag
    14101 ctccgccaca tgctactgtt ggctatccag gagtgctctg aaggctttgg gctggcctaa
    14161 taagcggccg cgacccagct ttcttgtaca aagtggttga tatcggtacc agtcgactct
    14221 agaggatccc gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt
    14281 tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga
    14341 ctaggtgtcc ttctataata ttatggggtg gagggggggt ggtatggagc aaggggcaag
    14401 ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagggc
    14461 acaatcttgg ctcactgcaa tctccgctct gggttcaagc gattctcctg cctcacctcc
    14521 gagttgtggg attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga
    14581 gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc
    14641 caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc
    14701 ttctgatttt aaaataacta taccagcagg aggacgtcca gacacagcat aggctacctg
    14761 ccatggccca accggtggga catttgagtt gcttgcttgg cactgtcctc tcatgcgttg
    14821 ggtccactca gtagatgcct gttgaattgg gtacgcggcc agcttctgtg gaatgtgtgt
    14881 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
    14941 ctcaattagt cagcaaccag gtgtggaaaa gtccccaggc tccccagcag gcagaagtat
    15001 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
    15061 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
    15121 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
    15181 ttttggaggc ctaggctttt gcaaaaagct cctcgaggaa ctgaaaaacc agaaagttaa
    15241 ttccctatag tgagtcgtat taaattcgta atcatgtcat agctgtttcc tgtgtgaaat
    15301 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
    15361 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
    15421 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
    15481 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    15541 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    15601 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    15661 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    15721 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    15781 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    15841 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
    15901 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    15961 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    16021 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    16081 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    16141 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    16201 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    16261 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    16321 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    16381 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    16441 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    16501 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    16561 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    16621 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    16681 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    16741 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    16801 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    16861 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    16921 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    16981 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    17041 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    17101 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    17161 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    17221 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    17281 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    17341 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    17401 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg
    17461 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
    17521 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
    17581 cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
    17641 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
    17701 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    17761 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
    17821 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aaacgtttac
    17881 aattt
//
