LOCUS Copy_of_pHAT2_hs_AKT1-P 5573 bp ds-DNA circular 26-MAY-2022 DEFINITION . FEATURES Location/Qualifiers CDS 230..889 /label="AmpR" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 rep_origin 987..1669 /label="ColE1 origin" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer_bind 1893..1912 /label="T7" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_binding 1911..1933 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc CDS 1978..2421 /label="hAKT1_PH_Domain" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 CDS 1978..3180 /label="Translation 1978-3180" CDS 2425..2436 /label="linker" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e CDS 2437..3153 /label="EGFP" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e CDS 3160..3177 /label="6xHis" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e promoter complement(3207..3225) /label="SP6 promoter" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer_bind complement(3208..3225) /label="SP6" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 CDS 3374..3442 /label="LacZ alpha" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc ORIGIN 1 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 61 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 121 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 181 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 241 taaagttctg ctatgtcata cactattatc ccgtattgac gccgggcaag agcaactcgg 301 tcgccgggcg cggtattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 361 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 421 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 481 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 541 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 601 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 661 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 721 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 781 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 841 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 901 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 961 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 1021 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 1081 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 1141 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 1201 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 1261 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 1321 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 1381 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 1441 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 1501 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 1561 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 1621 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 1681 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 1741 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 1801 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 1861 cgcgcgttgg ccgattcatt aatgcagctg attaatacga ctcactatag ggaattgtga 1921 gcggataaca attcctctag aaataatttt gtttaacttt aagaaggaga tatatccATG 1981 AGCGACGTGG CTATTGTGAA GGAGGGTTGG CTGCACAAAC GAGGGGAGTA CATCAAGACC 2041 TGGCGGCCAC GCTACTTCCT CCTCAAGAAT GATGGCACCT TCATTGGCTA CAAGGAGCGG 2101 CCGCAGGATG TGGACCAACG TGAGGCTCCC CTCAACAACT TCTCTGTGGC GCAGTGCCAG 2161 CTGATGAAGA CGGAGCGGCC CCGGCCCAAC ACCTTCATCA TCCGCTGCCT GCAGTGGACC 2221 ACTGTCATCG AACGCACCTT CCATGTGGAG ACTCCTGAGG AGCGGGAGGA GTGGACAACC 2281 GCCATCCAGA CTGTGGCTGA CGGCCTCAAG AAGCAGGAGG AGGAGGAGAT GGACTTCCGG 2341 TCGGGCTCAC CCAGTGACAA CTCAGGGGCT GAAGAGATGG AGGTGTCCCT GGCCAAGCCC 2401 AAGCACCGCG TGACCATGAA CGGACCGGTC GCCACCATGG TGAGCAAGGG CGAGGAGCTG 2461 TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC 2521 AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC 2581 TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC 2641 GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC 2701 ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG 2761 ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC 2821 ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC 2881 CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC 2941 CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC 3001 ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG 3061 AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC 3121 GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTCCGGAC ATCATCATCA TCATCACTGA 3181 GCggccgcat gcataagctt gagtattcta tagtgtcacc taaatcccag cttgatccgg 3241 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag 3301 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta 3361 tatccggata acctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 3421 gcgcagcctg aatggcgaat ggaaattgta aacgttaata ttttgttaaa attcgcgtta 3481 aatatttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 3541 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca 3601 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 3661 ccactacagc ttgcatgcct gcaggtcgga agcataaagt gtaaagcctg gggtgcctaa 3721 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 3781 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 3841 gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac 3901 cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa 3961 atcctgtttg atggtggttg acggcgggat ataacatgag ctgtcttcgg tatcgtcgta 4021 tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc 4081 gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag 4141 catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat 4201 cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga 4261 gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg 4321 ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg 4381 gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc 4441 atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt 4501 gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct 4561 ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag 4621 ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc 4681 cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt 4741 cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc 4801 atactctgcg acatcgtata acgttactgg tttcacattc accaccctga attgactctc 4861 ttccgggcgc tatcatgcca taccgcgaaa ggttttgcac cattcgatgg tgtcgggtac 4921 cgagctcgaa tttacgtgaa ccatcaccca aatcaagttt tttgcggtcg aggtgccgta 4981 aagctctaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 5041 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa 5101 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg 5161 gcgcgtcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata 5221 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg 5281 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa 5341 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc 5401 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg 5461 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 5521 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ata //