LOCUS       pCAGGS.seqT             5753 bp    DNA     circular SYN 01-Apr-2007
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     CDS             1..1628
                     /note="CAG promoter"
     CDS             join(1631..>1702,<2725..3228)
                     /note="rabbir beta-globin polyA [Split]"
     CDS             1703..1726
                     /note="pCAGGS-172 6"
     primer_bind     1739..2563
                     /note="miniTurbo"
     primer_bind     2564
                     /note="miniTurbo [Split]"
     CDS             2565..2621
                     /note="GGGS4"
     CDS             <2565..2573
                     /codon_start=3
                     /gene="AcGFP-[GGGS]x3"
                     /note=" [Split]"
     CDS             2700..2724
                     /note="pCAGS-1745Fw"
     CDS             3424..3760
                     /note="SV40 ori"
     CDS             complement(4754..5684)
                     /note="AmpR"
BASE COUNT      1237 A      1475 C      1624 G      1417 T         0 OTHER
ORIGIN      ?
        1 GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA
       61 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC
      121 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG
      181 GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC
      241 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG
      301 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG
      361 TATTAGTCAT CGCTATTACC ATGGTCGAGG TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA
      421 TCTCCCCCCC CTCCCCACCC CCAATTTTGT ATTTATTTAT TTTTTAATTA TTTTGTGCAG
      481 CGATGGGGGC GGGGGGGGGG GGGGGGCGCG CGCCAGGCGG GGCGGGGCGG GGCGAGGGGC
      541 GGGGCGGGGC GAGGCGGAGA GGTGCGGCGG CAGCCAATCA GAGCGGCGCG CTCCGAAAGT
      601 TTCCTTTTAT GGCGAGGCGG CGGCGGCGGC GGCCCTATAA AAAGCGAAGC GCGCGGCGGG
      661 CGGGAGTCGC TGCGCGCTGC CTTCGCCCCG TGCCCCGCTC CGCGCCGCCT CGCGCCGCCC
      721 GCCCCGGCTC TGACTGACCG CGTTACTCCC ACAGGTGAGC GGGCGGGACG GCCCTTCTCC
      781 TCCGGGCTGT AATTAGCGCT TGGTTTAATG ACGGCTTGTT TCTTTTCTGT GGCTGCGTGA
      841 AAGCCTTGAG GGGCTCCGGG AGGGCCCTTT GTGCGGGGGG AGCGGCTCGG GGGGTGCGTG
      901 CGTGTGTGTG TGCGTGGGGA GCGCCGCGTG CGGCTCCGCG CTGCCCGGCG GCTGTGAGCG
      961 CTGCGGGCGC GGCGCGGGGC TTTGTGCGCT CCGCAGTGTG CGCGAGGGGA GCGCGGCCGG
     1021 GGGCGGTGCC CCGCGGTGCG GGGGGGGCTG CGAGGGGAAC AAAGGCTGCG TGCGGGGTGT
     1081 GTGCGTGGGG GGGTGAGCAG GGGGTGTGGG CGCGTCGGTC GGGCTGCAAC CCCCCCTGCA
     1141 CCCCCCTCCC CGAGTTGCTG AGCACGGCCC GGCTTCGGGT GCGGGGCTCC GTACGGGGCG
     1201 TGGCGCGGGG CTCGCCGTGC CGGGCGGGGG GTGGCGGCAG GTGGGGGTGC CGGGCGGGGC
     1261 GGGGCCGCCT CGGGCCGGGG AGGGCTCGGG GGAGGGGCGC GGCGGCCCCC GGAGCGCCGG
     1321 CGGCTGTCGA GGCGCGGCGA GCCGCAGCCA TTGCCTTTTA TGGTAATCGT GCGAGAGGGC
     1381 GCAGGGACTT CCTTTGTCCC AAATCTGTGC GGAGCCGAAA TCTGGGAGGC GCCGCCGCAC
     1441 CCCCTCTAGC GGGCGCGGGG CGAAGCGGTG CGGCGCCGGC AGGAAGGAAA TGGGCGGGGA
     1501 GGGCCTTCGT GCGTCGCCGC GCCGCCGTCC CCTTCTCCCT CTCCAGCCTC GGGGCTGTCC
     1561 GCGGGGGGAC GGCTGCCTTC GGGGGGGACG GGGCAGGGCG GGGTTCGGCT TCTGGCGTGT
     1621 GACCGGCGGC TCTAGAGCCT CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT
     1681 CCTGGGCAAC GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAAGCTA GCGCCACCAT
     1741 GGCCTCTATG ACTGGTGGTC AACAAATGGG CCGGGGAAGC GAATTTATGA TTCCTCTTCT
     1801 GAACGCAAAG CAAATACTGG GTCAGCTCGA CGGGGGATCA GTCGCTGTTT TGCCTGTTGT
     1861 TGACTCTACT AATCAATATC TCTTGGATCG AATCGGTGAA CTCAAATCTG GCGATGCCTG
     1921 TATCGCCGAA TACCAACAGG CAGGTAGAGG TTCTAGAGGG AGAAAATGGT TCTCCCCGTT
     1981 TGGTGCCAAT CTCTATTTGA GTATGTTTTG GCGGCTTAAG CGAGGACCGG CGGCTATCGG
     2041 TCTCGGACCT GTTATTGGCA TCGTCATGGC CGAGGCGCTT AGGAAACTTG GAGCCGATAA
     2101 AGTCAGGGTC AAGTGGCCAA ATGATTTGTA TTTGCAAGAC AGAAAGCTCG CAGGCATCTT
     2161 GGTTGAGCTT GCTGGGATAA CGGGCGACGC AGCACAAATA GTCATTGGTG CCGGAATCAA
     2221 TGTCGCTATG CGCCGGGTTG AAGAAAGTGT CGTGAACCAA GGTTGGATAA CTCTTCAAGA
     2281 AGCGGGTATT AACCTCGATC GCAATACTCT CGCCGCTATG CTCATCAGGG AACTGCGAGC
     2341 GGCCCTGGAG TTGTTCGAGC AAGAAGGTCT CGCTCCCTAT TTGTCACGCT GGGAAAAGTT
     2401 GGATAATTTC ATTAACCGAC CAGTCAAACT TATTATTGGC GATAAAGAAA TATTTGGGAT
     2461 CTCACGCGGT ATCGATAAGC AAGGCGCATT GTTGCTTGAA CAGGATGGAG TGATTAAACC
     2521 ATGGATGGGA GGTGAGATCA GCCTTAGAAG TGCGGAAAAG AAATCCGGAC TCACCGGTGG
     2581 AGGCGGTTCA GGCGGAGGTG GCTCTGGCGG TGGCGGATCG AGATCTCGAG CTCAAGCTTC
     2641 GAATTCTGCA GTCGACGGTA CCGCGGGCCC GGGATCCACC GGATCTAGAT AACTGATCAA
     2701 CTCCTCAGGT GCAGGCTGCC TATCAGAAGG TGGTGGCTGG TGTGGCCAAT GCCCTGGCTC
     2761 ACAAATACCA CTGAGATCTT TTTCCCTCTG CCAAAAATTA TGGGGACATC ATGAAGCCCC
     2821 TTGAGCATCT GACTTCTGGC TAATAAAGGA AATTTATTTT CATTGCAATA GTGTGTTGGA
     2881 ATTTTTTGTG TCTCTCACTC GGAAGGACAT ATGGGAGGGC AAATCATTTA AAACATCAGA
     2941 ATGAGTATTT GGTTTAGAGT TTGGCAACAT ATGCCATATG CTGGCTGCCA TGAACAAAGG
     3001 TGGCTATAAA GAGGTCATCA GTATATGAAA CAGCCCCCTG CTGTCCATTC CTTATTCCAT
     3061 AGAAAAGCCT TGACTTGAGG TTAGATTTTT TTTATATTTT GTTTTGTGTT ATTTTTTTCT
     3121 TTAACATCCC TAAAATTTTC CTTACATGTT TTACTAGCCA GATTTTTCCT CCTCTCCTGA
     3181 CTACTCCCAG TCATAGCTGT CCCTCTTCTC TTATGAAGAT CCCTCGACCT GCAGCCCAAG
     3241 CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC
     3301 ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA
     3361 ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA
     3421 GCGGATCCGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG
     3481 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT
     3541 TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT
     3601 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA
     3661 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT
     3721 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCCGCTGC ATTAATGAAT
     3781 CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC
     3841 TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT
     3901 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA
     3961 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC
     4021 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     4081 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     4141 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCAATG
     4201 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     4261 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     4321 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     4381 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     4441 AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     4501 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     4561 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
     4621 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG
     4681 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
     4741 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT
     4801 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG
     4861 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC
     4921 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC
     4981 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
     5041 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC
     5101 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC
     5161 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA
     5221 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT
     5281 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
     5341 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA
     5401 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG
     5461 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC
     5521 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC
     5581 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
     5641 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA
     5701 GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTG
//
