LOCUS       pCAGGS.seqT             6131 bp    DNA     circular SYN 01-Apr-2007
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     source          <1739..>2116
                     /mol_type="other DNA"
                     /note=" [Split]"
                     /organism="synthetic DNA construct"
     CDS             1..1628
                     /note="CAG promoter"
     CDS             join(1631..>1702,<3103..3606)
                     /note="rabbir beta-globin polyA [Split]"
     CDS             1703..1726
                     /note="pCAGGS-172 6"
     CDS             1739..2116
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /label=H2B
                     /product="human histone H2B"
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             3078..3102
                     /note="pCAGS-1745Fw"
     CDS             3802..4138
                     /note="SV40 ori"
     CDS             complement(5132..6062)
                     /note="AmpR"
BASE COUNT      1331 A      1587 C      1734 G      1479 T         0 OTHER
ORIGIN      ?
        1 GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA
       61 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC
      121 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG
      181 GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC
      241 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG
      301 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG
      361 TATTAGTCAT CGCTATTACC ATGGTCGAGG TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA
      421 TCTCCCCCCC CTCCCCACCC CCAATTTTGT ATTTATTTAT TTTTTAATTA TTTTGTGCAG
      481 CGATGGGGGC GGGGGGGGGG GGGGGGCGCG CGCCAGGCGG GGCGGGGCGG GGCGAGGGGC
      541 GGGGCGGGGC GAGGCGGAGA GGTGCGGCGG CAGCCAATCA GAGCGGCGCG CTCCGAAAGT
      601 TTCCTTTTAT GGCGAGGCGG CGGCGGCGGC GGCCCTATAA AAAGCGAAGC GCGCGGCGGG
      661 CGGGAGTCGC TGCGCGCTGC CTTCGCCCCG TGCCCCGCTC CGCGCCGCCT CGCGCCGCCC
      721 GCCCCGGCTC TGACTGACCG CGTTACTCCC ACAGGTGAGC GGGCGGGACG GCCCTTCTCC
      781 TCCGGGCTGT AATTAGCGCT TGGTTTAATG ACGGCTTGTT TCTTTTCTGT GGCTGCGTGA
      841 AAGCCTTGAG GGGCTCCGGG AGGGCCCTTT GTGCGGGGGG AGCGGCTCGG GGGGTGCGTG
      901 CGTGTGTGTG TGCGTGGGGA GCGCCGCGTG CGGCTCCGCG CTGCCCGGCG GCTGTGAGCG
      961 CTGCGGGCGC GGCGCGGGGC TTTGTGCGCT CCGCAGTGTG CGCGAGGGGA GCGCGGCCGG
     1021 GGGCGGTGCC CCGCGGTGCG GGGGGGGCTG CGAGGGGAAC AAAGGCTGCG TGCGGGGTGT
     1081 GTGCGTGGGG GGGTGAGCAG GGGGTGTGGG CGCGTCGGTC GGGCTGCAAC CCCCCCTGCA
     1141 CCCCCCTCCC CGAGTTGCTG AGCACGGCCC GGCTTCGGGT GCGGGGCTCC GTACGGGGCG
     1201 TGGCGCGGGG CTCGCCGTGC CGGGCGGGGG GTGGCGGCAG GTGGGGGTGC CGGGCGGGGC
     1261 GGGGCCGCCT CGGGCCGGGG AGGGCTCGGG GGAGGGGCGC GGCGGCCCCC GGAGCGCCGG
     1321 CGGCTGTCGA GGCGCGGCGA GCCGCAGCCA TTGCCTTTTA TGGTAATCGT GCGAGAGGGC
     1381 GCAGGGACTT CCTTTGTCCC AAATCTGTGC GGAGCCGAAA TCTGGGAGGC GCCGCCGCAC
     1441 CCCCTCTAGC GGGCGCGGGG CGAAGCGGTG CGGCGCCGGC AGGAAGGAAA TGGGCGGGGA
     1501 GGGCCTTCGT GCGTCGCCGC GCCGCCGTCC CCTTCTCCCT CTCCAGCCTC GGGGCTGTCC
     1561 GCGGGGGGAC GGCTGCCTTC GGGGGGGACG GGGCAGGGCG GGGTTCGGCT TCTGGCGTGT
     1621 GACCGGCGGC TCTAGAGCCT CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT
     1681 CCTGGGCAAC GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAAGCTA GCGCCACCAT
     1741 GCCAGAGCCA GCGAAGTCTG CTCCCGCCCC GAAAAAGGGC TCCAAGAAGG CGGTGACTAA
     1801 GGCGCAGAAG AAAGGCGGCA AGAAGCGCAA GCGCAGCCGC AAGGAGAGCT ATTCCATCTA
     1861 TGTGTACAAG GTTCTGAAGC AGGTCCACCC TGACACCGGC ATTTCGTCCA AGGCCATGGG
     1921 CATCATGAAT TCGTTTGTGA ACGACATTTT CGAGCGCATC GCAGGTGAGG CTTCCCGCCT
     1981 GGCGCATTAC AACAAGCGCT CGACCATCAC CTCCAGGGAG ATCCAGACGG CCGTGCGCCT
     2041 GCTGCTGCCT GGGGAGTTGG CCAAGCACGC CGTGTCCGAG GGTACTAAGG CCATCACCAA
     2101 GTACACCAGC GCTAAGATGG CCTCTATGAC TGGTGGTCAA CAAATGGGCC GGGGAAGCGA
     2161 ATTTATGATT CCTCTTCTGA ACGCAAAGCA AATACTGGGT CAGCTCGACG GGGGATCAGT
     2221 CGCTGTTTTG CCTGTTGTTG ACTCTACTAA TCAATATCTC TTGGATCGAA TCGGTGAACT
     2281 CAAATCTGGC GATGCCTGTA TCGCCGAATA CCAACAGGCA GGTAGAGGTT CTAGAGGGAG
     2341 AAAATGGTTC TCCCCGTTTG GTGCCAATCT CTATTTGAGT ATGTTTTGGC GGCTTAAGCG
     2401 AGGACCGGCG GCTATCGGTC TCGGACCTGT TATTGGCATC GTCATGGCCG AGGCGCTTAG
     2461 GAAACTTGGA GCCGATAAAG TCAGGGTCAA GTGGCCAAAT GATTTGTATT TGCAAGACAG
     2521 AAAGCTCGCA GGCATCTTGG TTGAGCTTGC TGGGATAACG GGCGACGCAG CACAAATAGT
     2581 CATTGGTGCC GGAATCAATG TCGCTATGCG CCGGGTTGAA GAAAGTGTCG TGAACCAAGG
     2641 TTGGATAACT CTTCAAGAAG CGGGTATTAA CCTCGATCGC AATACTCTCG CCGCTATGCT
     2701 CATCAGGGAA CTGCGAGCGG CCCTGGAGTT GTTCGAGCAA GAAGGTCTCG CTCCCTATTT
     2761 GTCACGCTGG GAAAAGTTGG ATAATTTCAT TAACCGACCA GTCAAACTTA TTATTGGCGA
     2821 TAAAGAAATA TTTGGGATCT CACGCGGTAT CGATAAGCAA GGCGCATTGT TGCTTGAACA
     2881 GGATGGAGTG ATTAAACCAT GGATGGGAGG TGAGATCAGC CTTAGAAGTG CGGAAAAGAA
     2941 ATCCGGACTC ACCGGTGGAG GCGGTTCAGG CGGAGGTGGC TCTGGCGGTG GCGGATCGAG
     3001 ATCTCGAGCT CAAGCTTCGA ATTCTGCAGT CGACGGTACC GCGGGCCCGG GATCCACCGG
     3061 ATCTAGATAA CTGATCAACT CCTCAGGTGC AGGCTGCCTA TCAGAAGGTG GTGGCTGGTG
     3121 TGGCCAATGC CCTGGCTCAC AAATACCACT GAGATCTTTT TCCCTCTGCC AAAAATTATG
     3181 GGGACATCAT GAAGCCCCTT GAGCATCTGA CTTCTGGCTA ATAAAGGAAA TTTATTTTCA
     3241 TTGCAATAGT GTGTTGGAAT TTTTTGTGTC TCTCACTCGG AAGGACATAT GGGAGGGCAA
     3301 ATCATTTAAA ACATCAGAAT GAGTATTTGG TTTAGAGTTT GGCAACATAT GCCATATGCT
     3361 GGCTGCCATG AACAAAGGTG GCTATAAAGA GGTCATCAGT ATATGAAACA GCCCCCTGCT
     3421 GTCCATTCCT TATTCCATAG AAAAGCCTTG ACTTGAGGTT AGATTTTTTT TATATTTTGT
     3481 TTTGTGTTAT TTTTTTCTTT AACATCCCTA AAATTTTCCT TACATGTTTT ACTAGCCAGA
     3541 TTTTTCCTCC TCTCCTGACT ACTCCCAGTC ATAGCTGTCC CTCTTCTCTT ATGAAGATCC
     3601 CTCGACCTGC AGCCCAAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG
     3661 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG
     3721 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC
     3781 GGGAAACCTG TCGTGCCAGC GGATCCGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC
     3841 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC
     3901 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG
     3961 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTAAC TTGTTTATTG
     4021 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT
     4081 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGA
     4141 TCCGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC
     4201 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC
     4261 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA
     4321 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT
     4381 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     4441 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     4501 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     4561 CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     4621 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     4681 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     4741 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     4801 TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     4861 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     4921 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT
     4981 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
     5041 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
     5101 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA
     5161 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT
     5221 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG
     5281 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA
     5341 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA
     5401 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG
     5461 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC
     5521 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC
     5581 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA
     5641 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC
     5701 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG
     5761 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC
     5821 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG
     5881 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC
     5941 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT
     6001 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
     6061 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA
     6121 AGTGCCACCT G
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