LOCUS       Copy_of_pHAT2_dr_cPLA2- 5522 bp ds-DNA     circular     26-MAY-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             264..923
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      1021..1703
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     1927..1946
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    1945..1967
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     2012..2036
                     /label="EB_pr131F"
                     /ApEinfo_revcolor=#4444ff
                     /ApEinfo_fwdcolor=#4444ff
     CDS             2012..2404
                     /label="dr_cPLA2 C2 (Y90F, V91F) domain"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2012..3160
                     /label="dr_cPLA2-C2-Y90F-V91F-EGFP-6xHis"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             2012..3163
                     /label="Translation 2012-3163"
     misc_feature    2212..2212
                     /label="PCR\mut\A-->G"
                     /ApEinfo_revcolor=#4444ff
                     /ApEinfo_fwdcolor=#4444ff
     primer_bind     complement(2383..2404)
                     /label="EB_pr131R"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             2405..2419
                     /label="linker"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             2420..3136
                     /label="EGFP"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             3143..3160
                     /label="6xHis"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        complement(3190..3208)
                     /label="SP6 promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(3191..3208)
                     /label="SP6"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             3357..3425
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
       61 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      121 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      181 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      241 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt catacactat
      301 tatcccgtat tgacgccggg caagagcaac tcggtcgccg ggcgcggtat tctcagaatg
      361 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      421 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      481 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      541 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      601 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      661 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      721 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      781 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      841 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      901 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
      961 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1021 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1081 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1141 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1201 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     1261 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1321 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1381 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1441 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1501 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1561 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1621 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1681 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1741 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1801 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1861 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1921 gctgattaat acgactcact atagggaatt gtgagcggat aacaattcct ctagaaataa
     1981 ttttgtttaa ctttaagaag gagatatatc cATGTCCAAC ATTATAGTTG AGCATCAGTA
     2041 TTCTCATAGA CTGAAATTGA CTGTGGTGAG AGCTGAAAAT GTAACTAAAG GAGCATTTGG
     2101 GGATTTATTG GACACCCCGG ACCCTTATGT TGAGCTGTCA GTGCCAACCA CACCAGAGTC
     2161 AAGAAAACGA ACCCGCCACA TTAACAATGA CATTAACCCC AAGTGGAACG AGACCTTTGA
     2221 GTTCATATTG GATCCCAATC AATCCAATGT TTTGGAGGTG ACACTGATGG ATGCCAATTT
     2281 TTTCATGGAT GAAACTCTTG GCACAGCAAA ATATTCCCTC TCAAAACTCA AAGTGGCACA
     2341 AATGGAGCAT GTGACCCTCT CTATCGGCAA GACAACCAAA GTGTTCCTCG ACTTGCTCTT
     2401 GGAAGGACCG GTCGCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC
     2461 CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG
     2521 CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT
     2581 GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG
     2641 CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT
     2701 CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA
     2761 GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA
     2821 CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT
     2881 GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA
     2941 CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT
     3001 GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA
     3061 GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT
     3121 GGACGAGCTG TACAAGTCCG GACATCATCA TCATCATCAC TGAGCggccg catgcataag
     3181 cttgagtatt ctatagtgtc acctaaatcc cagcttgatc cggctgctaa caaagcccga
     3241 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
     3301 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg ataacctggc
     3361 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     3421 aatggaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaatattt gttaaatcag
     3481 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     3541 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     3601 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac agcttgcatg
     3661 cctgcaggtc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     3721 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     3781 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt
     3841 ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga
     3901 gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg
     3961 ttgacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatat
     4021 ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat
     4081 cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt
     4141 gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc
     4201 gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc
     4261 ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg
     4321 taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa
     4381 ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg
     4441 gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac
     4501 aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg
     4561 cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg
     4621 caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt
     4681 aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg
     4741 cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt
     4801 ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg
     4861 ccataccgcg aaaggttttg caccattcga tggtgtcggg taccgagctc gaatttacgt
     4921 gaaccatcac ccaaatcaag ttttttgcgg tcgaggtgcc gtaaagctct aaatcggaac
     4981 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     5041 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     5101 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcctgatgcg
     5161 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     5221 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     5281 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     5341 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     5401 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     5461 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     5521 aa
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