LOCUS       Copy_of_pHAT2_dr_cPLA2- 5286 bp ds-DNA     circular     26-MAY-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             103..762
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      860..1542
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     1766..1785
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    1784..1806
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             1851..2243
                     /label="dr_cPLA2 C2 domain"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             1851..2999
                     /label="dr_cPLA2_C2-EGFP-6xHis"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             1851..3002
                     /label="Translation 1851-3002"
     misc_feature    complement(2079..2123)
                     /label="pr008R"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2093..2134
                     /label="pr008F"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             2244..2258
                     /label="linker"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             2259..2975
                     /label="EGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             2982..2999
                     /label="6xHis"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        complement(3029..3047)
                     /label="SP6 promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(3030..3047)
                     /label="SP6"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             3196..3264
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
       61 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      121 ctgctatgtc atacactatt atcccgtatt gacgccgggc aagagcaact cggtcgccgg
      181 gcgcggtatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
      241 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
      301 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
      361 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
      421 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
      481 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
      541 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
      601 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
      661 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
      721 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
      781 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
      841 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
      901 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
      961 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1021 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1081 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1141 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1201 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1261 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1321 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1381 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1441 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1501 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1561 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     1621 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     1681 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     1741 tggccgattc attaatgcag ctgattaata cgactcacta tagggaattg tgagcggata
     1801 acaattcctc tagaaataat tttgtttaac tttaagaagg agatatatcc ATGTCCAACA
     1861 TTATAGTTGA GCATCAGTAT TCTCATAGAC TGAAATTGAC TGTGGTGAGA GCTGAAAATG
     1921 TAACTAAAGG AGCATTTGGG GATTTATTGC GCACCCCGGA CCCTTATGTT GAGCTGTCAG
     1981 TGCCAACCAC ACCAGAGTCA AGAAAACGAA CCCGCCACAT TAACAATGAC ATTAACCCCA
     2041 AGTGGAACGA GACCTTTGAG TTCATATTGG ATCCCAATCA ATCCAATGTT TTGGAGGTGA
     2101 CACTGATGCG TGCCAATTAT GTCATGGATG AAACTCTTGG CACAGCAAAA TATTCCCTCT
     2161 CAAAACTCAA AGTGGCACAA ATGGAGCATG TGACCCTCTC TATCGGCAAG ACAACCAAAG
     2221 TGTTCCTCGA CTTGCTCTTG GAAGGACCGG TCGCCACCAT GGTGAGCAAG GGCGAGGAGC
     2281 TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT
     2341 TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA
     2401 TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG
     2461 GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG
     2521 CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA
     2581 AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG
     2641 GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA
     2701 GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA
     2761 TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC
     2821 CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC
     2881 TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG
     2941 CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACATCATCAT CATCATCACT
     3001 GAGCggccgc atgcataagc ttgagtattc tatagtgtca cctaaatccc agcttgatcc
     3061 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
     3121 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
     3181 tatatccgga taacctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     3241 ttgcgcagcc tgaatggcga atggaaattg taagcgttaa tattttgtta aaattcgcgt
     3301 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     3361 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     3421 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     3481 gcccacagct tgcatgcctg caggtcggaa gcataaagtg taaagcctgg ggtgcctaat
     3541 gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     3601 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcagc ccggactcgg taatggcgcg
     3661 cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc
     3721 attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc
     3781 cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg
     3841 cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac
     3901 cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg
     3961 tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc
     4021 aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag
     4081 aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac
     4141 cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc
     4201 gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg
     4261 ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg
     4321 cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac
     4381 accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg
     4441 actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcaccatt cgatggtgtc
     4501 gggtaccgag ctcgaattta cgtgaaccat cacccaaatc aagttttttg cggtcgaggt
     4561 gccgtaaagc tctaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     4621 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     4681 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     4741 tacagggcgc gtcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     4801 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     4861 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     4921 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     4981 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     5041 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5101 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5161 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5221 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5281 gtaaaa
//