LOCUS       Copy_of_pHAT2_dr_alox5- 5483 bp ds-DNA     circular     02-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             103..762
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      860..1542
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1425..1444
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     1766..1785
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    1784..1806
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             1851..2204
                     /label="dr_alox5a_PLAT"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             1851..2963
                     /label="Translation 1851-2963"
     misc_feature    1877..1896
                     /label="054f aLOX5f"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(2079..2098)
                     /label="054r aLOX5r"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             2208..2219
                     /label="linker"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2220..2237
                     /label="011_mTq2-no_RS_F"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(2220..2237)
                     /label="011_mTq2-no_RS_F_RC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             2220..2936
                     /label="EGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2223..2240
                     /label="QAQA_TS_F"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(2261..2279)
                     /label="pr043r EGFP-N1 rev"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     Primer          complement(2265..2286)
                     /label="EGFP-N"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(2266..2286)
                     /label="EGFP-N"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2286..2308
                     /label="sGFP2_to_mClover3_C_2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(2291..2308)
                     /label="sGFP2_to_mClover3_B_2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2363..2381
                     /label="RhoA_wt_mScarletI_SGFP2_FOR_2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Primer          2465..2485
                     /label="5'_KpnI_mClover3"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2467..2485
                     /label="5’ KpnI_mClover3_2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(2560..2578)
                     /label="hCD4-for1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2573..2592
                     /label="CTS40R_For1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(2573..2592)
                     /label="CTS40R_Rev1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(2589..2608)
                     /label="cpYFP_D"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2591..2627
                     /label="cpYFP_E"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2634..2654
                     /label="GCamp6s_D_RC "
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(2634..2654)
                     /label="GCamp6s_D"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2873..2891
                     /label="EGFP-C1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2873..2894
                     /label="EGFP-C"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(2873..2894)
                     /label="EGFP-C_RC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(2898..2931)
                     /label="cpYFP_B"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2906..2924
                     /label="pr087f pEGFPC1for"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(2922..2942)
                     /label="eGFP_D"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             2943..2960
                     /label="6xHis"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        complement(2990..3008)
                     /label="SP6 promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(2991..3008)
                     /label="SP6"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(2991..3008)
                     /label="Sp6"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(2991..3009)
                     /label="SP6"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(3066..3084)
                     /label="T7-Term"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             3157..3225
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    5137..5159
                     /label="3GEX"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(5137..5159)
                     /label="3GEX-RC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
       61 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      121 ctgctatgtc atacactatt atcccgtatt gacgccgggc aagagcaact cggtcgccgg
      181 gcgcggtatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
      241 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
      301 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
      361 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
      421 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
      481 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
      541 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
      601 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
      661 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
      721 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
      781 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
      841 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
      901 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
      961 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1021 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1081 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1141 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1201 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1261 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1321 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1381 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1441 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1501 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1561 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     1621 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     1681 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     1741 tggccgattc attaatgcag ctgattaata cgactcacta tagggaattg tgagcggata
     1801 acaattcctc tagaaataat tttgtttaac tttaagaagg agatatatcc ATGCCCAGCT
     1861 ACACGGTGAC TGTGGCCACT GGCACTCAGT GGTTTGCTGG CACCGATGAT TATATTTATC
     1921 TGACTCTGGT GGGCTCTGAG GGCTGCAGTG ACAGGACCCT GCTGGATAAA CCCCTCTACA
     1981 ACGACTTCGA GAGAGGAGCG GTGGACTCAT ATGACATCAG CGTAGGAGAG GATCTTGGTG
     2041 AAATTCAGCT GGTTAAAATT GAAAAGAAGA AATACTGGAT GCACGACGAC TGGTACTGTA
     2101 AATACATCAC GGTGAAGACA CCCTATGGAG ATTATGTGGA GTTCCCCTGC TTCCGCTGGT
     2161 TGGTTGATGA CAAAGAGGTG ATCCTCAGAG ATGGCAGGGC TCGAGGACCG GTCGCCACCA
     2221 TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG
     2281 GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG
     2341 GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC
     2401 TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC
     2461 AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT
     2521 TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG
     2581 TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA
     2641 AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG
     2701 GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG
     2761 ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT
     2821 ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC
     2881 TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTCCG
     2941 GACATCATCA TCATCATCAC TGAGCggccg catgcataag cttgagtatt ctatagtgtc
     3001 acctaaatcc cagcttgatc cggctgctaa caaagcccga aaggaagctg agttggctgc
     3061 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
     3121 ttttttgctg aaaggaggaa ctatatccgg ataacctggc gtaatagcga agaggcccgc
     3181 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt gtaaacgtta
     3241 atattttgtt aaaattcgcg ttaaatattt gttaaatcag ctcatttttt aaccaatagg
     3301 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     3361 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     3421 aaaccgtcta tcagggcgat ggcccactac agcttgcatg cctgcaggtc ggaagcataa
     3481 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     3541 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     3601 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg
     3661 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
     3721 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttgacggcgg gatataacat
     3781 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
     3841 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
     3901 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
     3961 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
     4021 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
     4081 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa
     4141 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
     4201 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
     4261 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
     4321 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
     4381 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
     4441 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
     4501 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
     4561 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca
     4621 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
     4681 caccattcga tggtgtcggg taccgagctc gaatttacgt gaaccatcac ccaaatcaag
     4741 ttttttgcgg tcgaggtgcc gtaaagctct aaatcggaac cctaaaggga gcccccgatt
     4801 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     4861 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     4921 cgcgcttaat gcgccgctac agggcgcgtc gcctgatgcg gtattttctc cttacgcatc
     4981 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     5041 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     5101 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     5161 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     5221 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     5281 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5341 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5401 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5461 cagaaacgct ggtgaaagta aaa
//