LOCUS       Copy_of_pHAT2_dr_alox12 5480 bp ds-DNA     circular     02-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             103..762
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      860..1542
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     1766..1785
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    1784..1806
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             1784..1850
                     /label="Translation 1784-1850"
     exon            1851..1994
                     /label="ENSDART00000101126 Exon 3"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1851..2201
                     /label="PLAT Domain"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1851..2960
                     /label="Translation 1851-2960"
     exon            1995..2199
                     /label="ENSDART00000101126 Exon 4"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             2205..2216
                     /label="linker"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             2217..2933
                     /label="EGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             2940..2957
                     /label="6xHis"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        complement(2987..3005)
                     /label="SP6 promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(2988..3005)
                     /label="SP6"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             3154..3222
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
       61 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      121 ctgctatgtc atacactatt atcccgtatt gacgccgggc aagagcaact cggtcgccgg
      181 gcgcggtatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
      241 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
      301 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
      361 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
      421 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
      481 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
      541 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
      601 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
      661 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
      721 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
      781 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
      841 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
      901 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
      961 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1021 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1081 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1141 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1201 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1261 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1321 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1381 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1441 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1501 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1561 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     1621 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     1681 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     1741 tggccgattc attaatgcag ctgattaata cgactcacta tagggaattg tgagcggata
     1801 acaattcctc tagaaataat tttgtttaac tttaagaagg agatatatcc ATGGAGTACA
     1861 AAGTGACAGT GGCCACCGGG ACTTCTGAAT ATTCAGGGAC CAATAATTAT GTGTACGTGA
     1921 CTCTGATTGG AGAGAAAGGA AAGAGCGAGA GGACGCTGCT GGACAATCCT GGCCTGGACT
     1981 TCTGCAGAGG AGCTGTGGAT GATTACATAG TTAAAAGCGA TACTGATTTG GGCCCCCTGA
     2041 TTCTGGTGCG ACTGGAGAAG CATAAGTACT TCATAGAAGA CAACTGGTTC TGCCGCTACG
     2101 TGAAAGTCAG TGTACCTGGA GAAAGATGTT GTTACACATT TCCTTGTTAC CGCTGGCTTG
     2161 TGGGAGATGG AGTGTTTGTA GAGCTCAGAG AAGGCACAGC CGGACCGGTC GCCACCATGG
     2221 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG
     2281 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA
     2341 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG
     2401 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC
     2461 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA
     2521 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA
     2581 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC
     2641 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA
     2701 TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC
     2761 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC
     2821 TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC
     2881 TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTCCGGAC
     2941 ATCATCATCA TCATCACTGA GCggccgcat gcataagctt gagtattcta tagtgtcacc
     3001 taaatcccag cttgatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
     3061 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     3121 tttgctgaaa ggaggaacta tatccggata acctggcgta atagcgaaga ggcccgcacc
     3181 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggaaattgta aacgttaata
     3241 ttttgttaaa attcgcgtta aatatttgtt aaatcagctc attttttaac caataggccg
     3301 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     3361 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     3421 ccgtctatca gggcgatggc ccactacagc ttgcatgcct gcaggtcgga agcataaagt
     3481 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     3541 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     3601 ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc
     3661 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
     3721 gtttgcccca gcaggcgaaa atcctgtttg atggtggttg acggcgggat ataacatgag
     3781 ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac
     3841 tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg
     3901 ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag
     3961 tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca
     4021 gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg
     4081 tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata
     4141 atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag
     4201 gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg
     4261 acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct
     4321 accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca
     4381 atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt
     4441 ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct
     4501 tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg
     4561 gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc
     4621 accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcac
     4681 cattcgatgg tgtcgggtac cgagctcgaa tttacgtgaa ccatcaccca aatcaagttt
     4741 tttgcggtcg aggtgccgta aagctctaaa tcggaaccct aaagggagcc cccgatttag
     4801 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     4861 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     4921 gcttaatgcg ccgctacagg gcgcgtcgcc tgatgcggta ttttctcctt acgcatctgt
     4981 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     5041 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     5101 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     5161 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     5221 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     5281 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5341 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5401 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5461 aaacgctggt gaaagtaaaa
//