LOCUS       Exported                7940 bp DNA     circular SYN 12-MAY-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Universitaetsklinikum Halle
  TITLE     Direct Submission
  JOURNAL   Exported Jun 3, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7940
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     protein_bind    235..256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        271..301
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    309..325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     333..349
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        370..388
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        416..642
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             643..823
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    870..995
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1488..1721
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2217..2334
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2368..2774
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    2858..3444
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             3445..4146
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     misc_feature    4381..4784
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             4874..5107
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5179..5300
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5340..5475
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5496..5514)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5524..5540)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5682..6137
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6163..6267
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6268..7128
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7299..7887
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
       61 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      121 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      181 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      241 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      301 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      361 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
      421 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
      481 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
      541 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
      601 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
      661 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
      721 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
      781 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
      841 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
      901 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
      961 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     1021 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     1081 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     1141 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     1201 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     1261 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     1321 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     1381 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     1441 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     1501 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     1561 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     1621 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     1681 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     1741 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     1801 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     1861 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     1921 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     1981 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     2041 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     2101 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     2161 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt
     2221 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     2281 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttatcgat
     2341 cacgagacta gcctcgagct agctgcagta acgccatttt gcaaggcatg gaaaaatacc
     2401 aaaccaagaa tagagaagtt cagatcaagg gcgggtacat gaaaatagct aacgttgggc
     2461 caaacaggat atctgcggtg agcagtttcg gccccggccc ggggccaaga acagatggtc
     2521 accgcagttt cggccccggc ccgaggccaa gaacagatgg tccccagata tggcccaacc
     2581 ctcagcagtt tcttaagacc catcagatgt ttccaggctc ccccaaggac ctgaaatgac
     2641 cctgcgcctt atttgaatta accaatcagc ctgcttctcg cttctgttcg cgcgcttctg
     2701 cttcccgagc tctataaaag agctcacaac ccctcactcg gcgcgccagt cctccgacag
     2761 actgagtcgg ccggtcgaat caagcttatc gataccgtcg acggatccac cggtactagt
     2821 atactccggc gcgtatgcat gagtaactga ggatccgccc ctctccctnc ccccccccta
     2881 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     2941 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     3001 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     3061 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     3121 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     3181 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     3241 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     3301 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     3361 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     3421 cacgatgata atatggccac aaccatggtg agcaagggcg aggaggtcat caaagagttc
     3481 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     3541 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     3601 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
     3661 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
     3721 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
     3781 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc
     3841 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac
     3901 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc
     3961 cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc
     4021 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg
     4081 gaacagtacg agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg
     4141 tacaagtaaa gcggccgcgt cgacggatcc cccgggctgc aggaattcga gcatcttacc
     4201 gccatttatt cccatatttg ttctgttttt cttgatttgg gtatacattt aaatgttaat
     4261 aaaacaaaat ggtggggcaa tcatttacat ttttagggat atgtaattac tagttcaggt
     4321 gtattgccac aagacaaaca tgttaagaaa ctttcccgtt atttacgctc tgttcctgtt
     4381 aatcaacctc tggattacaa aatttgtgaa agattgactg atattcttaa ctatgttgct
     4441 ccttttacgc tgtgtggata tgctgcttta atgcctctgt atcatgctat tgcttcccgt
     4501 acggctttcg ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4561 tggcccgttg tccgtcaacg tggcgtggtg tgctctgtgt ttgctgacgc aacccccact
     4621 ggctggggca ttgccaccac ctgtcaactc ctttctggga ctttcgcttt ccccctcccg
     4681 atcgccacgg cagaactcat cgccgcctgc cttgcccgct gctggacagg ggctaggttg
     4741 ctgggcactg ataattccgt ggtgttgtcg gggaagctga cgtcctttcg aattcgatat
     4801 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     4861 gaaaaggggg gactggaagg gctacgtaac tcccaacgaa gacaagatct gctttttgct
     4921 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4981 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5041 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     5101 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     5161 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5221 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5281 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     5341 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     5401 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     5461 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     5521 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     5581 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     5641 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     5701 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5761 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5821 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5881 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5941 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     6001 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     6061 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     6121 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6181 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6241 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6301 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6361 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6421 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6481 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6541 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6601 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6661 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6721 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6781 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6841 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6901 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6961 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7021 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7081 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7141 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7201 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7261 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7321 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7381 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7441 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7501 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7561 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7621 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7681 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7741 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7801 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7861 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7921 gccttttgct ggccttttgc
//
