LOCUS       Exported               10533 bp DNA     circular SYN 05-JUN-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 5, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        51..627
                     /label=CMV_immearly_promoter
                     /note="color: #85dae9"
     misc_feature    130..417
                     /label=CAG_enhancer
                     /note="color: #ffef86"
     promoter        585..654
                     /label=CMV_promoter
                     /note="color: #b4abac"
     promoter        597..716
                     /label=CMV2_promoter
                     /note="color: #ff9ccd"
     CDS             735..5882
                     /label=Translation 735-5882
                     /note="color: #993366; direction: RIGHT"
     gene            735..4838
                     /label=Cas9
                     /note="color: #faac61; direction: RIGHT"
     CDS             4851..4871
                     /label=SV40 NLS
                     /note="color: #b7e6d7; direction: RIGHT"
     misc_feature    4878..4932
                     /label=T2A
                     /note="color: #d6b295"
     gene            4938..5882
                     /label=miRFP670
                     /note="color: #f58a5e; direction: RIGHT"
     terminator      5906..6176
                     /label=TK_PA_terminator
                     /note="color: #f8d3a9"
     misc_feature    6230..6536
                     /label=f1_origin
                     /note="color: #c7b0e3"
     promoter        6668..6936
                     /label=SV40_promoter
                     /note="color: #b4abac"
     gene            7054..7842
                     /label=NeoR/KanR
                     /note="color: #75c6a9; direction: RIGHT"
     terminator      8024..8143
                     /label=SV40_PA_terminator
                     /note="color: #ff9ccd"
     misc_feature    complement(8578..9197)
                     /label=pBR322_origin
                     /note="color: #ffef86; direction: LEFT"
     gene            complement(9352..10212)
                     /label=Ampicillin
                     /note="color: #f58a5e; direction: LEFT"
     promoter        complement(10254..10282)
                     /label=AmpR_promoter
                     /note="color: #b1ff67; direction: LEFT"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actctagagc caccatggac aagaagtact ccattgggct cgatatcggc acaaacagcg
      781 tcggctgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc aaagttctgg
      841 gcaataccga tcgccacagc ataaagaaga acctcattgg cgccctcctg ttcgactccg
      901 gggagacggc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat acccgcagaa
      961 agaatcggat ctgctacctg caggagatct ttagtaatga gatggctaag gtggatgact
     1021 ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag cacgagcgcc
     1081 acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac ccaaccatat
     1141 atcatctgag gaagaagctt gtagacagta ctgataaggc tgacttgcgg ttgatctatc
     1201 tcgcgctggc gcatatgatc aaatttcggg gacacttcct catcgagggg gacctgaacc
     1261 cagacaacag cgatgtcgac aaactcttta tccaactggt tcagacttac aatcagcttt
     1321 tcgaagagaa cccgatcaac gcatccggag ttgacgccaa agcaatcctg agcgctaggc
     1381 tgtccaaatc ccggcggctc gaaaacctca tcgcacagct ccctggggag aagaagaacg
     1441 gcctgtttgg taatcttatc gccctgtcac tcgggctgac ccccaacttt aaatctaact
     1501 tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga cacctacgat gatgatctcg
     1561 acaatctgct ggcccagatc ggcgaccagt acgcagacct ttttttggcg gcaaagaacc
     1621 tgtcagacgc cattctgctg agtgatattc tgcgagtgaa cacggagatc accaaagctc
     1681 cgctgagcgc tagtatgatc aagcgctatg atgagcacca ccaagacttg actttgctga
     1741 aggcccttgt cagacagcaa ctgcctgaga agtacaagga aattttcttc gatcagtcta
     1801 aaaatggcta cgccggatac attgacggcg gagcaagcca ggaggaattt tacaaattta
     1861 ttaagcccat cttggaaaaa atggacggca ccgaggagct gctggtaaag cttaacagag
     1921 aagatctgtt gcgcaaacag cgcactttcg acaatggaag catcccccac cagattcacc
     1981 tgggcgaact gcacgctatc ctcaggcggc aagaggattt ctaccccttt ttgaaagata
     2041 acagggaaaa gattgagaaa atcctcacat ttcggatacc ctactatgta ggccccctcg
     2101 cccggggaaa ttccagattc gcgtggatga ctcgcaaatc agaagagacc atcactccct
     2161 ggaacttcga ggaagtcgtg gataaggggg cctctgccca gtccttcatc gaaaggatga
     2221 ctaactttga taaaaatctg cctaacgaaa aggtgcttcc taaacactct ctgctgtacg
     2281 agtacttcac agtttataac gagctcacca aggtcaaata cgtcacagaa gggatgagaa
     2341 agccagcatt cctgtctgga gagcagaaga aagctatcgt ggacctcctc ttcaagacga
     2401 accggaaagt taccgtgaaa cagctcaaag aagactattt caaaaagatt gaatgtttcg
     2461 actctgttga aatcagcgga gtggaggatc gcttcaacgc atccctggga acgtatcacg
     2521 atctcctgaa aatcattaaa gacaaggact tcctggacaa tgaggagaac gaggacattc
     2581 ttgaggacat tgtcctcacc cttacgttgt ttgaagatag ggagatgatt gaagaacgct
     2641 tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa acagctcaag aggcgccgat
     2701 atacaggatg ggggcggctg tcaagaaaac tgatcaatgg gatccgagac aagcagagtg
     2761 gaaagacaat cctggatttt cttaagtccg atggatttgc caaccggaac ttcatgcagt
     2821 tgatccatga tgactctctc acctttaagg aggacatcca gaaagcacaa gtttctggcc
     2881 agggggacag tcttcacgag cacatcgcta atcttgcagg tagcccagct atcaaaaagg
     2941 gaatactgca gaccgttaag gtcgtggatg aactcgtcaa agtaatggga aggcataagc
     3001 ccgagaatat cgttatcgag atggcccgag agaaccaaac tacccagaag ggacagaaga
     3061 acagtaggga aaggatgaag aggattgaag agggtataaa agaactgggg tcccaaatcc
     3121 ttaaggaaca cccagttgaa aacacccagc ttcagaatga gaagctctac ctgtactacc
     3181 tgcagaacgg cagggacatg tacgtggatc aggaactgga catcaatcgg ctctccgact
     3241 acgacgtgga tcatatcgtg ccccagtctt ttctcaaaga tgattctatt gataataaag
     3301 tgttgacaag atccgataaa aatagaggga agagtgataa cgtcccctca gaagaagttg
     3361 tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc caaactgatc acacaacgga
     3421 agttcgataa tctgactaag gctgaacgag gtggcctgtc tgagttggat aaagccggct
     3481 tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa gcacgtggcc caaattctcg
     3541 attcacgcat gaacaccaag tacgatgaaa atgacaaact gattcgagag gtgaaagtta
     3601 ttactctgaa gtctaagctg gtctcagatt tcagaaagga ctttcagttt tataaggtga
     3661 gagagatcaa caattaccac catgcgcatg atgcctacct gaatgcagtg gtaggcactg
     3721 cacttatcaa aaaatatccc aagcttgaat ctgaatttgt ttacggagac tataaagtgt
     3781 acgatgttag gaaaatgatc gcaaagtctg agcaggaaat aggcaaggcc accgctaagt
     3841 acttctttta cagcaatatt atgaattttt tcaagaccga gattacactg gccaatggag
     3901 agattcggaa gcgaccactt atcgaaacaa acggagaaac aggagaaatc gtgtgggaca
     3961 agggtaggga tttcgcgaca gtccggaagg tcctgtccat gccgcaggtg aacatcgtta
     4021 aaaagaccga agtacagacc ggaggcttct ccaaggaaag tatcctcccg aaaaggaaca
     4081 gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa gaaatacggc ggattcgatt
     4141 ctcctacagt cgcttacagt gtactggttg tggccaaagt ggagaaaggg aagtctaaaa
     4201 aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat ggagcgatca agcttcgaaa
     4261 aaaaccccat cgactttctc gaggcgaaag gatataaaga ggtcaaaaaa gacctcatca
     4321 ttaagcttcc caagtactct ctctttgagc ttgaaaacgg ccggaaacga atgctcgcta
     4381 gtgcgggcga gctgcagaaa ggtaacgagc tggcactgcc ctctaaatac gttaatttct
     4441 tgtatctggc cagccactat gaaaagctca aagggtctcc cgaagataat gagcagaagc
     4501 agctgttcgt ggaacaacac aaacactacc ttgatgagat catcgagcaa ataagcgaat
     4561 tctccaaaag agtgatcctc gccgacgcta acctcgataa ggtgctttct gcttacaata
     4621 agcacaggga taagcccatc agggagcagg cagaaaacat tatccacttg tttactctga
     4681 ccaacttggg cgcgcctgca gccttcaagt acttcgacac caccatagac agaaagcggt
     4741 acacctctac aaaggaggtc ctggacgcca cactgattca tcagtcaatt acggggctct
     4801 atgaaacaag aatcgacctc tctcagctcg gtggagacag cagggctgac cccaagaaga
     4861 agaggaaggt ggctagcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga
     4921 atcccggccc tggtaccgta gcaggtcatg cctctggcag ccccgcattc gggaccgcct
     4981 ctcattcgaa ttgcgaacat gaagagatcc acctcgccgg ctcgatccag ccgcatggcg
     5041 cgcttctggt cgtcagcgaa catgatcatc gcgtcatcca ggccagcgcc aacgccgcgg
     5101 aatttctgaa tctcggaagc gtactcggcg ttccgctcgc cgagatcgac ggcgatctgt
     5161 tgatcaagat cctgccgcat ctcgatccca ccgccgaagg catgccggtc gcggtgcgct
     5221 gccggatcgg caatccctct acggagtact gcggtctgat gcatcggcct ccggaaggcg
     5281 ggctgatcat cgaactcgaa cgtgccggcc cgtcgatcga tctgtcaggc acgctggcgc
     5341 cggcgctgga gcggatccgc acggcgggtt cactgcgcgc gctgtgcgat gacaccgtgc
     5401 tgctgtttca gcagtgcacc ggctacgacc gggtgatggt gtatcgtttc gatgagcaag
     5461 gccacggcct ggtattctcc gagtgccatg tgcctgggct cgaatcctat ttcggcaacc
     5521 gctatccgtc gtcgactgtc ccgcagatgg cgcggcagct gtacgtgcgg cagcgcgtcc
     5581 gcgtgctggt cgacgtcacc tatcagccgg tgccgctgga gccgcggctg tcgccgctga
     5641 ccgggcgcga tctcgacatg tcgggctgct tcctgcgctc gatgtcgccg tgccatctgc
     5701 agttcctgaa ggacatgggc gtgcgcgcca ccctggcggt gtcgctggtg gtcggcggca
     5761 agctgtgggg cctggttgtc tgtcaccatt atctgccgcg cttcatccgt ttcgagctgc
     5821 gggcgatctg caaacggctc gccgaaagga tcgcgacgcg gatcaccgcg cttgagagct
     5881 aaaccggtta gtaatgagtt taaacggggg aggctaactg aaacacggaa ggagacaata
     5941 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg
     6001 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     6061 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     6121 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcaga
     6181 tctgcgcagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     6241 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6301 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6361 aaatcggggc atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6421 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6481 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6541 caaccctatc tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg
     6601 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     6661 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6721 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6781 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     6841 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6901 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     6961 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     7021 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     7081 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     7141 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     7201 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     7261 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     7321 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     7381 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     7441 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     7501 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     7561 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     7621 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     7681 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     7741 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     7801 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     7861 tcgcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     7921 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     7981 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     8041 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     8101 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     8161 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8221 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8281 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8341 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8401 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8461 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8521 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8581 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8641 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8701 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8761 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     8821 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8881 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8941 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9001 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     9061 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9121 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9181 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9241 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9301 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9361 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9421 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9481 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9541 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9601 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9661 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9721 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9781 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9841 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9901 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9961 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10021 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10081 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10141 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10201 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10261 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10321 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
    10381 tcgactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
    10441 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
    10501 ttgaccgaca attgcatgaa gaatctgctt agg
//
