LOCUS       Exported                9594 bp DNA     circular SYN 06-JUN-2022
DEFINITION  pJR101_direct_capture_ucoe_ef1a_puroegfp_sgb2m (1)  (9594 bp).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 6, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        247..626
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        628..826
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             844..1024
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1071..1196
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1695..1928
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2113..2157
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2455..2572
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2825..2858
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        3812..4023
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          4024..4962
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             5589..5642
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    6394..6427
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    6483..7071
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6954..6965)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7074..7090)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             7600..7780
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7842..8430)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8601..9461)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9462..9566)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
       61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
      121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
      181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
      241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct
     1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
     2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc
     2641 gccatctcta ggcccgcgcc ggccccctcg cacggacttg tgggagaagc tcggctactc
     2701 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga
     2761 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat
     2821 ttctataact tcgtatagca tacattatac gaagttataa acagcacaaa aggaaactca
     2881 ccctaactgt aaagtaattg tgtgttttga gactataagt atcccttgga gaaccacctt
     2941 gttgggcgag cacagctaag gccagtttaa gagctaagct ggaaacagca tagcaagttt
     3001 aaataaggct agtccgttat caacttggcc gctttaaggc cggtcctagc aaggccaagt
     3061 ggcaccgagt cggtgctttt tttctcgagt actaggatcc attaggcggc cgccgggagg
     3121 tggtccctgc agttacgcca atgataaccc ccgccagaaa aatcttagta gccttccctt
     3181 tttgttttcc gtgccccaac tcggcggatt gactcggccc cttccggaaa cacccgaatc
     3241 aacttctagt caaattattg ttcacgccgc aatgacccac ccctggcccg cgtctgtgga
     3301 actgacccct ggtgtacagg agagttcgct gctgaaagtg gtcccaaagg ggtactagtt
     3361 tttaagctcc caactccccc tcccccagcg tctggaggat tccacaccct cgcaccgcag
     3421 gggcgaggaa gtgggcggag tccggttttg gcgccagccg ctgaggctgc caagcagaaa
     3481 agccaccgct gaggagactc cggtcactgt cctcgccccg cctccccctt ccctcccctt
     3541 ggggaccacc gggcgccacg ccgcgaacgg taagtgccgc ggtcgtcggc gcctccgccc
     3601 tccccctagg gccccaattc ccagcgggcg cggcgcgcgg cccctccccc cgccgggcgc
     3661 gcgcccgctg ccccgccctt cgtggccgcc cggcgtgggc ggtgccaccc ctccccccgg
     3721 cggcccccct gcagccgcgc gcagctcccg gctccctccc ccttcggatg tggcttgagc
     3781 tgtaggcgcg gagatgcatg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     3841 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
     3901 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     3961 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     4021 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
     4081 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
     4141 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
     4201 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
     4261 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
     4321 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
     4381 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
     4441 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
     4501 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
     4561 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
     4621 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
     4681 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
     4741 aggcacctcg attagttctc gcgcttttgg agtacgtcgt ctttaggttg gggggagggg
     4801 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
     4861 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
     4921 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg agctagccca
     4981 ccatgaccga gtacaagccc accgttcgcc tggccacaag agatgacgtg cctcgcgccg
     5041 tgagaacttt ggctgcggcg ttcgccgatt atccagcaac caggcacaca gtggaccctg
     5101 atagacacat cgagcgagtg actgagctcc aggaactgtt tctgacacgg gttggtctgg
     5161 atatcggaaa agtgtgggtc gctgacgatg gggccgccgt ggcagtctgg acaaccccag
     5221 aatccgtgga ggccggagca gtattcgccg agataggccc ccgcatggcg gagctttcgg
     5281 ggtcaaggct ggctgcacag cagcaaatgg agggcctact cgctccacac cggcctaaag
     5341 aacccgcctg gttcctagcc actgttggcg tgtcaccgga ccatcaggga aaagggctgg
     5401 gctctgctgt ggtgctgcct ggtgtcgagg ctgcggagag ggccggagtg ccggcttttc
     5461 ttgaaactag cgcacctagg aacttacctt tctacgagcg tctgggattt accgtcacag
     5521 ccgacgtgga ggttccagag gggccccgga cttggtgtat gacacggaaa ccgggcgcag
     5581 gttctgggga gggtaggggg tcacttctga cgtgcgggga tgtggaagaa aaccccggcc
     5641 caccagtagc aacgatggtc agtaagggcg aggaactttt tacaggggtc gttcccattc
     5701 tggttgaatt ggatggcgat gtgaacggac ataaatttag cgtgtccggc gaaggagaag
     5761 gtgatgctac ctacggaaag cttacgttga agtttatttg cacgaccggt aagctgcccg
     5821 taccatggcc aacattggtg accactctga cctatggagt gcagtgtttc agccggtacc
     5881 ccgaccacat gaaacaacac gacttcttca agtctgccat gcctgaaggt tatgtccagg
     5941 aacgtaccat cttttttaaa gacgacggaa attataagac ccgagccgag gtaaagtttg
     6001 agggcgacac tctcgtcaac cgcatagaat tgaaggggat tgacttcaaa gaagatggca
     6061 acattctagg ccataaatta gagtataatt acaacagcca caacgtttac atcatggctg
     6121 acaaacagaa gaatggcatc aaggcaaatt tcaagataag acacaatatc gaggacggga
     6181 gtgtccaact ggccgaccat tatcagcaga atacacctat tggcgacgga cctgtcctcc
     6241 tccccgacaa tcattacctg agtactcaat ccgcactctc caaagatccc aacgagaaga
     6301 gagatcacat ggtattactg gagttcgtga cagctgccgg gatcaccctc ggcatggatg
     6361 aactctacaa gtgagaattc gtcgagggac ctaataactt cgtatagcat acattatacg
     6421 aagttataca tgtttaaggg ttccggttcc actaggtaca attcgatatc aagcttatcg
     6481 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     6541 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6601 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6661 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6721 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     6781 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     6841 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     6901 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     6961 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     7021 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     7081 gtcgacctcg atcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     7141 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     7201 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7261 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     7321 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     7381 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     7441 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
     7501 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
     7561 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
     7621 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7681 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7741 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatgtgagc aaaaggccag
     7801 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7861 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7921 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7981 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8041 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8101 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8161 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8221 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8281 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8341 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8401 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8461 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8521 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8581 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8641 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8701 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8761 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8821 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8881 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8941 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9001 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9061 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9121 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9181 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9241 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9301 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9361 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9421 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9481 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9541 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
