LOCUS       Exported                9568 bp DNA     circular SYN 06-JUN-2022
DEFINITION  pjr104_fill_5_pjr92_uncr1_ucoe  (9568 bp).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 6, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        247..626
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        628..826
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             844..1024
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1071..1196
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1695..1928
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2113..2157
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2455..2572
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2825..2858
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     gap             2946..2964
                     /estimated_length=19
     promoter        3786..3997
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3998..4936
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             5563..5616
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    6368..6401
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    6457..7045
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6928..6939)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7048..7064)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             7574..7754
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7816..8404)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8575..9435)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9436..9540)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
       61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
      121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
      181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
      241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct
     1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
     2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc
     2641 gccatctcta ggcccgcgcc ggccccctcg cacggacttg tgggagaagc tcggctactc
     2701 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga
     2761 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat
     2821 ttctataact tcgtatagca tacattatac gaagttataa acagcacaaa aggaaactca
     2881 ccctaactgt aaagtaattg tgtgttttga gactataagt atcccttgga gaaccacctt
     2941 gttggnnnnn nnnnnnnnnn nnnngtttaa gagctaagct ggaaacagca tagcaagttt
     3001 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttctc
     3061 gagtactagg atccattagg cggccgccgg gaggtggtcc ctgcagttac gccaatgata
     3121 acccccgcca gaaaaatctt agtagccttc cctttttgtt ttccgtgccc caactcggcg
     3181 gattgactcg gccccttccg gaaacacccg aatcaacttc tagtcaaatt attgttcacg
     3241 ccgcaatgac ccacccctgg cccgcgtctg tggaactgac ccctggtgta caggagagtt
     3301 cgctgctgaa agtggtccca aaggggtact agtttttaag ctcccaactc cccctccccc
     3361 agcgtctgga ggattccaca ccctcgcacc gcaggggcga ggaagtgggc ggagtccggt
     3421 tttggcgcca gccgctgagg ctgccaagca gaaaagccac cgctgaggag actccggtca
     3481 ctgtcctcgc cccgcctccc ccttccctcc ccttggggac caccgggcgc cacgccgcga
     3541 acggtaagtg ccgcggtcgt cggcgcctcc gccctccccc tagggcccca attcccagcg
     3601 ggcgcggcgc gcggcccctc cccccgccgg gcgcgcgccc gctgccccgc ccttcgtggc
     3661 cgcccggcgt gggcggtgcc acccctcccc ccggcggccc ccctgcagcc gcgcgcagct
     3721 cccggctccc tcccccttcg gatgtggctt gagctgtagg cgcggagatg catgtgcccg
     3781 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     3841 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     3901 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     3961 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     4021 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     4081 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     4141 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     4201 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     4261 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     4321 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     4381 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     4441 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     4501 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     4561 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     4621 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     4681 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgcgctt
     4741 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     4801 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     4861 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     4921 ttttcttcca tttcaggtgt cgtgagctag cccaccatga ccgagtacaa gcccaccgtt
     4981 cgcctggcca caagagatga cgtgcctcgc gccgtgagaa ctttggctgc ggcgttcgcc
     5041 gattatccag caaccaggca cacagtggac cctgatagac acatcgagcg agtgactgag
     5101 ctccaggaac tgtttctgac acgggttggt ctggatatcg gaaaagtgtg ggtcgctgac
     5161 gatggggccg ccgtggcagt ctggacaacc ccagaatccg tggaggccgg agcagtattc
     5221 gccgagatag gcccccgcat ggcggagctt tcggggtcaa ggctggctgc acagcagcaa
     5281 atggagggcc tactcgctcc acaccggcct aaagaacccg cctggttcct agccactgtt
     5341 ggcgtgtcac cggaccatca gggaaaaggg ctgggctctg ctgtggtgct gcctggtgtc
     5401 gaggctgcgg agagggccgg agtgccggct tttcttgaaa ctagcgcacc taggaactta
     5461 cctttctacg agcgtctggg atttaccgtc acagccgacg tggaggttcc agaggggccc
     5521 cggacttggt gtatgacacg gaaaccgggc gcaggttctg gggagggtag ggggtcactt
     5581 ctgacgtgcg gggatgtgga agaaaacccc ggcccaccag tagcaacgat ggtcagtaag
     5641 ggcgaggaac tttttacagg ggtcgttccc attctggttg aattggatgg cgatgtgaac
     5701 ggacataaat ttagcgtgtc cggcgaagga gaaggtgatg ctacctacgg aaagcttacg
     5761 ttgaagttta tttgcacgac cggtaagctg cccgtaccat ggccaacatt ggtgaccact
     5821 ctgacctatg gagtgcagtg tttcagccgg taccccgacc acatgaaaca acacgacttc
     5881 ttcaagtctg ccatgcctga aggttatgtc caggaacgta ccatcttttt taaagacgac
     5941 ggaaattata agacccgagc cgaggtaaag tttgagggcg acactctcgt caaccgcata
     6001 gaattgaagg ggattgactt caaagaagat ggcaacattc taggccataa attagagtat
     6061 aattacaaca gccacaacgt ttacatcatg gctgacaaac agaagaatgg catcaaggca
     6121 aatttcaaga taagacacaa tatcgaggac gggagtgtcc aactggccga ccattatcag
     6181 cagaatacac ctattggcga cggacctgtc ctcctccccg acaatcatta cctgagtact
     6241 caatccgcac tctccaaaga tcccaacgag aagagagatc acatggtatt actggagttc
     6301 gtgacagctg ccgggatcac cctcggcatg gatgaactct acaagtgaga attcgtcgag
     6361 ggacctaata acttcgtata gcatacatta tacgaagtta tacatgttta agggttccgg
     6421 ttccactagg tacaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
     6481 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6541 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6601 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6661 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6721 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6781 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6841 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     6901 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6961 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7021 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgatcgag acctagaaaa
     7081 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     7141 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     7201 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     7261 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     7321 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
     7381 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
     7441 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     7501 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
     7561 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7621 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7681 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7741 gaaaatctct agcagcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7801 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7861 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7921 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7981 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8041 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8101 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8161 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8221 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     8281 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8341 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8401 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8461 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8521 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8581 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8641 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8701 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8761 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8821 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8881 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8941 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9001 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9061 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9121 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9181 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9241 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9301 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9361 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9421 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9481 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9541 cacatttccc cgaaaagtgc cacctgac
//
