LOCUS       Exported               10917 bp DNA     circular SYN 21-MAY-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10917)
  AUTHORS   Institute for Molecular Bioscience
  TITLE     Direct Submission
  JOURNAL   Exported Jun 7, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    771..791
                     /label=cmv-f
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1685..1918
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2445..2562
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2617..2887
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     misc_feature    2931..2990
                     /label=Anillin shRNA
     misc_feature    2954..2962
                     /label=shRNA loop
     primer_bind     2993..3009
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3051..3084
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     enhancer        3164..3467
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3468..3671
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3700..3709
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3706..4416
                     /codon_start=1
                     /label=mCherry
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKP"
     CDS             4423..7686
                     /codon_start=1
                     /label=Anillin A740D, E758K
                     /translation="MDPFTEKLLERTRARRENLQRKMAERPTAAPRSMTHAKRARQPLS
                     EASNQQPLSGGEEKSCTKPSPSKKRCSDNTEVEVSNLENKQPVESTSAKSCSPSPVSPQ
                     VQPQAADTISDSVAVPASLLGMRRGLNSRLEATAASSVKTRMQKLAEQRRRWDNDDMTD
                     DIPESSLFSPMPSEEKAASPPRPLLSNASATPVGRRGRLANLAATICSWEDDVNHSFAK
                     QNSVQEQPGTACLSKFSSASGASARINSSSVKQEATFCSQRDGDASLNKALSSSAADAS
                     LVNASISSSVKATSPVKSTTSITDAKSCEGQNPELLPKTPISPLKTGVSKPIVKSTLSQ
                     TVPSKGELSREICLQSQSKDKSTTPGGTGIKPFLERFGERCQEHSKESPARSTPHRTPI
                     ITPNTKAIQEKLFKQDTSSSTTHLAQQLKQERQKELACLRGRFDKGNIWSAEKGGNSKS
                     KQLETKQETHCQSTPLKKHQGVSKTQSLPVTEKVTENQIPAKNSSTEPKEVIREIEMSV
                     DDDDINSSKVINDLFSDVLEEGELDMEKSQEEMDQALAESSEEQEDALNISSMSLLAPL
                     AQTVGVVSPESLVSTPRLELKDTSRSDESPKPGKFQRTRVPRAESGDSLGSEDRDLLYS
                     IDAYRSQRFKETERPSIKQVIVRKEDVTSKLDEKNNAFPCQVNIKQKMQELNNEINMQQ
                     TVIYQASQDLNCCVDEEHGKGSLEEAKAERLLLIATGKRTLLIDELNKLKNEGPQRKNK
                     ASPQSEFMPSKGSVTLSEIRLPLKADFVCSTVQKPDAANYYYLIILKAGAENMVATPLA
                     STSNSLNGDALTFTTTFTLQDVSNDFEINIEVYSLVQKKDPSGLDKKKKTSKSKAITPK
                     RLLTSITTKSNIHSSVMASPGGLSAVRTSNFALVGSYTLSLSSVGNTKFVLDKVPFLSS
                     LEGHIYLKIKCQVNSSVEERGFLTIFEDVSGFGAWHRRWCVLSGNCISYWTYPDDEKRK
                     NPIGRINLANCTSRQIEPANREFCARRNTFELITVRPQREDDRETLVSQCRDTLCVTKN
                     WLSADTKEERDLWMQKLNQVLVDIRLWQPDACYKPIGKP"
     protein_bind    7714..7747
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    8169..8393
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8274..8285)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8396..8412)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8922..9102
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(9164..9752)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9923..10783)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10784..10888)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct ggaggaggag
     1561 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1621 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1681 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1741 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     1801 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1861 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1921 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1981 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     2041 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     2101 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     2161 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     2221 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     2281 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     2341 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     2401 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2461 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2521 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2581 agcagagatc cagtttggtt agtaccgcgc tctagagatc cgacgccgcc atctctaggc
     2641 ccgcgccggc cccctcgcac agacttgtgg gagaagctcg gctactcccc tgccccggtt
     2701 aatttgcata taatatttcc tagtaactat agaggcttaa tgtgcgataa aagacagata
     2761 atctgttctt tttaatacta gctacatttt acatgatagg cttggatttc tataagagat
     2821 acaaatacta aattattatt ttaaaaaaca gcacaaaagg aaactcaccc taactgtaaa
     2881 gtaattgtgt gttttgagac tataaatatc ccttggagaa aagccttgtt tcggcgatgc
     2941 ctctttgaat aaattcaaga gatttattca aagaggcatc gccatttttt ctcgaggtcg
     3001 acggtatcga taagctcgct tcacgagatt ccagcaggtc gagggaccta ataacttcgt
     3061 atagcataca ttatacgaag ttatattaag ggttccaagc ttaagcggcc gcatagtaat
     3121 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     3181 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     3241 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     3301 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     3361 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     3421 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     3481 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     3541 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     3601 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     3661 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ccaccatggt gagcaagggc
     3721 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     3781 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     3841 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     3901 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     3961 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     4021 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     4081 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     4141 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     4201 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     4261 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     4321 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     4381 cactccaccg gcggcatgga cgagctgtac aagccgccgc ggatggatcc gtttacggag
     4441 aaactgctgg agcgaacccg tgccaggcga gagaatcttc agagaaaaat ggctgagagg
     4501 cccacagcag ctccaaggtc tatgactcat gctaagcgag ctagacagcc actttcagaa
     4561 gcaagtaacc agcagcccct ctctggtggt gaagagaaat cttgtacaaa accatcgcca
     4621 tcaaaaaaac gctgttctga caacactgaa gtagaagttt ctaacttgga aaataaacaa
     4681 ccagttgagt cgacatctgc aaaatcttgt tctccaagtc ctgtgtctcc tcaggtgcag
     4741 ccacaagcag cagataccat cagtgattct gttgctgtcc cggcatcact gctgggcatg
     4801 aggagagggc tgaactcaag attggaagca actgcagcct cctcagttaa aacacgtatg
     4861 caaaaacttg cagagcaacg gcgccgttgg gataatgatg atatgacaga tgacattcct
     4921 gaaagctcac tcttctcacc aatgccatca gaggaaaagg ctgcttcccc tcccagacct
     4981 ctgctttcaa atgcctcggc aactccagtt ggcagaaggg gccgtctggc caatcttgct
     5041 gcaactattt gctcctggga agatgatgta aatcactcat ttgcaaaaca aaacagtgta
     5101 caagaacagc ctggtaccgc ttgtttatcc aaattttcct ctgcaagtgg agcatctgct
     5161 aggatcaata gcagcagtgt taagcaggaa gctacattct gttcccaaag ggatggcgac
     5221 gcaagcttaa acaaggccct atcctcaagt gctgctgatg cgtctttggt taatgcctca
     5281 atttccagct ctgtgaaagc tacttctcca gtgaaatcta ctacatctat cactgatgct
     5341 aaaagttgtg agggacaaaa tcctgagcta cttccaaaaa ctcctattag tcctctgaaa
     5401 acgggggtat cgaaaccaat tgtgaagtca actttatccc agacagttcc atccaaggga
     5461 gaattaagta gagaaatttg tctgcaatct caatctaaag acaaatctac gacaccagga
     5521 ggaacaggaa ttaagccttt cctggaacgc tttggagagc gttgtcaaga acatagcaaa
     5581 gaaagtccag ctcgtagcac accccacaga acccccatta ttactccaaa tacaaaggcc
     5641 atccaagaaa aattattcaa gcaagacaca tcttcatcta ctacccattt agcacaacag
     5701 ctcaagcagg aacgtcaaaa agaactagca tgtcttcgtg gccgatttga caagggcaat
     5761 atatggagtg cagaaaaagg cggaaactca aaaagcaaac aactagaaac caaacaggaa
     5821 actcactgtc agagcactcc cctcaaaaaa caccaaggtg tttcaaaaac tcagtcactt
     5881 ccagtaacag aaaaggtgac cgaaaaccag ataccagcca aaaattctag tacagaacct
     5941 aaagaagtga tacgtgaaat tgagatgagt gtggatgatg atgatatcaa tagttcgaaa
     6001 gtaattaatg acctcttcag tgatgtccta gaggaaggtg aactagatat ggagaagagc
     6061 caagaggaga tggatcaagc attagcagaa agcagcgaag aacaggaaga tgcactgaat
     6121 atctcctcaa tgtctttact tgcaccattg gcacaaacag ttggtgtggt aagtccagag
     6181 agtttagtgt ccacacctag actggaattg aaagacacca gcagaagtga tgaaagtcca
     6241 aaaccaggaa aattccaaag aactcgtgtc cctcgagctg aatctggtga tagccttggt
     6301 tctgaagatc gtgatcttct ttacagcatt gatgcatata gatctcaaag attcaaagaa
     6361 acagaacgtc catcaataaa gcaggtgatt gttcggaagg aagatgttac ttcaaaactg
     6421 gatgaaaaaa ataatgcctt tccttgtcaa gttaatatca aacagaaaat gcaggaactc
     6481 aataacgaaa taaatatgca acagacagtg atctatcaag ctagccagga tcttaactgc
     6541 tgtgttgatg aagaacatgg aaaagggtcc ctagaagaag ctaaagcaga aagacttctt
     6601 ctaattgcaa ctgggaagag aacacttttg attgatgaat tgaataaatt gaagaacgaa
     6661 ggacctcaga ggaagaataa ggctagtccc caaagtgaat ttatgccatc caaaggatca
     6721 gttactttgt cagaaatccg cttgcctcta aaagcagatt ttgtctgcag tacggttcag
     6781 aaaccagatg cagcaaatta ctattactta attatactaa aagcaggagc tgaaaatatg
     6841 gtagccacac cattagcaag tacttcaaac tctcttaacg gtgatgctct gacattcact
     6901 actacattta ctctgcaaga tgtatccaat gactttgaaa taaatattga agtttacagc
     6961 ttggtgcaaa agaaagatcc ctcaggcctt gataagaaga aaaaaacatc caagtccaag
     7021 gctattactc caaagcgact cctcacatct ataaccacaa aaagcaacat tcattcttca
     7081 gtcatggcca gtccaggagg tcttagtgct gtgcgaacca gcaacttcgc ccttgttgga
     7141 tcttacacat tatcattgtc ttcagtagga aatactaagt ttgttctgga caaggtcccc
     7201 tttttatctt ctttggaagg tcatatttat ttaaaaataa aatgtcaagt gaattccagt
     7261 gttgaagaaa gaggttttct aaccatattt gaagatgtta gtggttttgg tgcctggcat
     7321 cgaagatggt gtgttctttc tggaaactgt atatcttatt ggacttatcc agatgatgag
     7381 aaacgcaaga atcccatagg aaggataaat ctggctaatt gtaccagtcg tcagatagaa
     7441 ccagccaaca gagaattttg tgcaagacgc aacacttttg aattaattac tgtccgacca
     7501 caaagagaag atgaccgaga gactcttgtc agccaatgca gggacacact ctgtgttacc
     7561 aagaactggc tgtctgcaga tactaaagaa gagcgggatc tctggatgca aaaactcaat
     7621 caagttcttg ttgatattcg cctctggcaa cctgatgctt gctacaaacc tattggaaag
     7681 ccttaacctg caggcaattc gtcgagggac ctaataactt cgtatagcat acattatacg
     7741 aagttataca tgtttaaggg ttccggttcc actaggtaca attcgatatc aagcttatcg
     7801 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     7861 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     7921 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     7981 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     8041 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     8101 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     8161 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     8221 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     8281 tcaatccagc ggaccttcct tcccgcgcgg cctgctgccg gctctgcggc ctcttccgcg
     8341 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     8401 ccgtcgacct cgatcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     8461 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     8521 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     8581 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     8641 ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag
     8701 ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     8761 aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc
     8821 tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag
     8881 catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc
     8941 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     9001 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     9061 gatccctcag acccttttag tcagtgtgga aaatctctag cagcatgtga gcaaaaggcc
     9121 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     9181 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9241 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9301 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9361 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9421 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9481 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9541 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9601 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9661 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9721 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9781 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9841 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9901 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9961 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    10021 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    10081 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    10141 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    10201 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    10261 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    10321 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    10381 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    10441 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10501 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10561 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10621 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10681 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10741 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10801 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10861 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacg
//
