LOCUS       Exported               10898 bp DNA     circular SYN 18-OCT-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pll5.0-Anillin shRNA-EGFP-Anillin A740D, E758K
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10898)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jun 7, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2932..2991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          4402..7665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate earlypromoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virustype
                     1"
     misc_feature    1686..1919
                     /label=RRE
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     primer_bind     1900..1919
                     /label=RRE_3' FW
     misc_feature    2446..2563
                     /label=cPPT/CTS
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central 
                     terminationsequence of HIV-1"
     promoter        2618..2931
                     /label=U6 promoter
                     /note="U6 promoter"
                     /note="RNA polymerase III promoter for mouse U6 snRNA 
                     (Daset al., 1988)"
     misc_feature    2929..2997
                     /label=shrna insert between Hpa1 and Xho1
     misc_feature    2932..2991
                     /label=Anillin ShRNA
     primer_bind     2994..3010
                     /label=KS primer
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3032..3051)
                     /label=Between KS and Cre Rv
     protein_bind    3052..3085
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     LTR             3114..3627
     misc_feature    3629..3671
                     /label=MCS
     misc_feature    3673..3682
                     /label=Kozak sequence
     CDS             3679..4395
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=enhanced GFP
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    4402..7665
                     /label=Human Anillin DM
                     /note="A740D, E758K"
     protein_bind    7696..7729
                     /label=lox p:Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    7785..8375
                     /label=WPRE
                     /note="WPRE"
                     /note="woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
     primer_bind     complement(7785..7804)
                     /label=WPRE Rv
     CDS             complement(8256..8267)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa recognition and cleavage site
                     /note="Factor Xa site"
                     /translation="IEGR"
     primer_bind     complement(8378..8394)
                     /label=KS primer
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8904..9084
                     /label=5' LTR (truncated)
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(9146..9734)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9905..10765)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=ampr
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10766..10870)
                     /gene="bla"
                     /label=Ampr promoter
                     /note="AmpR promoter"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga
     1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2581 cagcagagat ccagtttggt tagtaccgcg ctctagagat ccgacgccgc catctctagg
     2641 cccgcgccgg ccccctcgca cagacttgtg ggagaagctc ggctactccc ctgccccggt
     2701 taatttgcat ataatatttc ctagtaacta tagaggctta atgtgcgata aaagacagat
     2761 aatctgttct ttttaatact agctacattt tacatgatag gcttggattt ctataagaga
     2821 tacaaatact aaattattat tttaaaaaac agcacaaaag gaaactcacc ctaactgtaa
     2881 agtaattgtg tgttttgaga ctataaatat cccttggaga aaagccttgt ttcggcgatg
     2941 cctctttgaa taaattcaag agatttattc aaagaggcat cgccattttt tctcgaggtc
     3001 gacggtatcg ataagctcgc ttcacgagat tccagcaggt cgagggacct aataacttcg
     3061 tatagcatac attatacgaa gttatattaa gggttccaag cttaagcggc cgctgaaaga
     3121 ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaat
     3181 acataactga gaatagagaa gttcagatca aggttaggaa cagagagaca gcagaatatg
     3241 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     3301 gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg tttccagggt
     3361 gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc
     3421 gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc
     3481 ggcgcgccag tcctccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
     3541 ttgctgtttg catccgaatc gtggactcgc tgatccttgg gagggtctcc tcagattgat
     3601 tgactgccca cctcgggggt ctttcatcga attctgcagt cgacggtacc gcgggcccgg
     3661 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     3721 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     3781 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     3841 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     3901 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     3961 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     4021 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     4081 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     4141 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     4201 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     4261 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     4321 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     4381 gacgagctgt acaagccgcg gatggatccg tttacggaga aactgctgga gcgaacccgt
     4441 gccaggcgag agaatcttca gagaaaaatg gctgagaggc ccacagcagc tccaaggtct
     4501 atgactcatg ctaagcgagc tagacagcca ctttcagaag caagtaacca gcagcccctc
     4561 tctggtggtg aagagaaatc ttgtacaaaa ccatcgccat caaaaaaacg ctgttctgac
     4621 aacactgaag tagaagtttc taacttggaa aataaacaac cagttgagtc gacatctgca
     4681 aaatcttgtt ctccaagtcc tgtgtctcct caggtgcagc cacaagcagc agataccatc
     4741 agtgattctg ttgctgtccc ggcatcactg ctgggcatga ggagagggct gaactcaaga
     4801 ttggaagcaa ctgcagcctc ctcagttaaa acacgtatgc aaaaacttgc agagcaacgg
     4861 cgccgttggg ataatgatga tatgacagat gacattcctg aaagctcact cttctcacca
     4921 atgccatcag aggaaaaggc tgcttcccct cccagacctc tgctttcaaa tgcctcggca
     4981 actccagttg gcagaagggg ccgtctggcc aatcttgctg caactatttg ctcctgggaa
     5041 gatgatgtaa atcactcatt tgcaaaacaa aacagtgtac aagaacagcc tggtaccgct
     5101 tgtttatcca aattttcctc tgcaagtgga gcatctgcta ggatcaatag cagcagtgtt
     5161 aagcaggaag ctacattctg ttcccaaagg gatggcgacg caagcttaaa caaggcccta
     5221 tcctcaagtg ctgctgatgc gtctttggtt aatgcctcaa tttccagctc tgtgaaagct
     5281 acttctccag tgaaatctac tacatctatc actgatgcta aaagttgtga gggacaaaat
     5341 cctgagctac ttccaaaaac tcctattagt cctctgaaaa cgggggtatc gaaaccaatt
     5401 gtgaagtcaa ctttatccca gacagttcca tccaagggag aattaagtag agaaatttgt
     5461 ctgcaatctc aatctaaaga caaatctacg acaccaggag gaacaggaat taagcctttc
     5521 ctggaacgct ttggagagcg ttgtcaagaa catagcaaag aaagtccagc tcgtagcaca
     5581 ccccacagaa cccccattat tactccaaat acaaaggcca tccaagaaaa attattcaag
     5641 caagacacat cttcatctac tacccattta gcacaacagc tcaagcagga acgtcaaaaa
     5701 gaactagcat gtcttcgtgg ccgatttgac aagggcaata tatggagtgc agaaaaaggc
     5761 ggaaactcaa aaagcaaaca actagaaacc aaacaggaaa ctcactgtca gagcactccc
     5821 ctcaaaaaac accaaggtgt ttcaaaaact cagtcacttc cagtaacaga aaaggtgacc
     5881 gaaaaccaga taccagccaa aaattctagt acagaaccta aagaagtgat acgtgaaatt
     5941 gagatgagtg tggatgatga tgatatcaat agttcgaaag taattaatga cctcttcagt
     6001 gatgtcctag aggaaggtga actagatatg gagaagagcc aagaggagat ggatcaagca
     6061 ttagcagaaa gcagcgaaga acaggaagat gcactgaata tctcctcaat gtctttactt
     6121 gcaccattgg cacaaacagt tggtgtggta agtccagaga gtttagtgtc cacacctaga
     6181 ctggaattga aagacaccag cagaagtgat gaaagtccaa aaccaggaaa attccaaaga
     6241 actcgtgtcc ctcgagctga atctggtgat agccttggtt ctgaagatcg tgatcttctt
     6301 tacagcattg atgcatatag atctcaaaga ttcaaagaaa cagaacgtcc atcaataaag
     6361 caggtgattg ttcggaagga agatgttact tcaaaactgg atgaaaaaaa taatgccttt
     6421 ccttgtcaag ttaatatcaa acagaaaatg caggaactca ataacgaaat aaatatgcaa
     6481 cagacagtga tctatcaagc tagccaggat cttaactgct gtgttgatga agaacatgga
     6541 aaagggtccc tagaagaagc taaagcagaa agacttcttc taattgcaac tgggaagaga
     6601 acacttttga ttgatgaatt gaataaattg aagaacgaag gacctcagag gaagaataag
     6661 gctagtcccc aaagtgaatt tatgccatcc aaaggatcag ttactttgtc agaaatccgc
     6721 ttgcctctaa aagcagattt tgtctgcagt acggttcaga aaccagatgc agcaaattac
     6781 tattacttaa ttatactaaa agcaggagct gaaaatatgg tagccacacc attagcaagt
     6841 acttcaaact ctcttaacgg tgatgctctg acattcacta ctacatttac tctgcaagat
     6901 gtatccaatg actttgaaat aaatattgaa gtttacagct tggtgcaaaa gaaagatccc
     6961 tcaggccttg ataagaagaa aaaaacatcc aagtccaagg ctattactcc aaagcgactc
     7021 ctcacatcta taaccacaaa aagcaacatt cattcttcag tcatggccag tccaggaggt
     7081 cttagtgctg tgcgaaccag caacttcgcc cttgttggat cttacacatt atcattgtct
     7141 tcagtaggaa atactaagtt tgttctggac aaggtcccct ttttatcttc tttggaaggt
     7201 catatttatt taaaaataaa atgtcaagtg aattccagtg ttgaagaaag aggttttcta
     7261 accatatttg aagatgttag tggttttggt gcctggcatc gaagatggtg tgttctttct
     7321 ggaaactgta tatcttattg gacttatcca gatgatgaga aacgcaagaa tcccatagga
     7381 aggataaatc tggctaattg taccagtcgt cagatagaac cagccaacag agaattttgt
     7441 gcaagacgca acacttttga attaattact gtccgaccac aaagagaaga tgaccgagag
     7501 actcttgtca gccaatgcag ggacacactc tgtgttacca agaactggct gtctgcagat
     7561 actaaagaag agcgggatct ctggatgcaa aaactcaatc aagttcttgt tgatattcgc
     7621 ctctggcaac ctgatgcttg ctacaaacct attggaaagc cttgataacc tgcaggcaat
     7681 tcgtcgaggg acctaataac ttcgtatagc atacattata cgaagttata catgtttaag
     7741 ggttccggtt ccactaggta caattcgata tcaagcttat cgataatcaa cctctggatt
     7801 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     7861 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     7921 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     7981 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     8041 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     8101 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     8161 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     8221 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     8281 cttcccgcgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     8341 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgatcgag
     8401 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     8461 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     8521 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     8581 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     8641 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     8701 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     8761 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     8821 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     8881 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8941 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     9001 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     9061 agtcagtgtg gaaaatctct agcagcatgt gagcaaaagg ccagcaaaag gccaggaacc
     9121 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9181 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9241 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9301 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9361 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9421 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9481 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9541 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     9601 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9661 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9721 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9781 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     9841 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     9901 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     9961 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    10021 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10081 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    10141 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10201 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10261 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10321 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10381 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10441 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    10501 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    10561 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    10621 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    10681 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    10741 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    10801 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    10861 gggttccgcg cacatttccc cgaaaagtgc cacctgac
//
