LOCUS       Exported               10614 bp DNA     circular SYN 10-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   Institute for Molecular Bioscience
  TITLE     Direct Submission
  JOURNAL   Exported Jun 7, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    772..792
                     /label=cmv-f
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1686..1919
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2446..2563
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2618..2888
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     misc_feature    2932..2991
                     /label=Anillin shRNA
     misc_feature    2955..2963
                     /label=shRNA loop
     primer_bind     2994..3010
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3052..3085
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     enhancer        3165..3468
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3469..3672
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3701..3710
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3707..4417
                     /codon_start=1
                     /label=mCherry
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKP"
     CDS             4421..7384
                     /codon_start=1
                     /label=Anillin-Delta-Myo
                     /translation="MDPFTEKLLERTRARRENLQRKMAERPTAAPRSMTHAKRARQPLS
                     EASNQQPLSGGEEKSCTKPSPSKKRCSDNTEVEVSNLENKQPVESTSAKSCSPSPVSPQ
                     VQPQAADTISDSVAVPASLLGMRRGLNSRLEATAASSVKTRMQKLASSVKQEATFCSQR
                     DGDASLNKALSSSAADASLVNASISSSVKATSPVKSTTSITDAKSCEGQNPELLPKTPI
                     SPLKTGVSKPIVKSTLSQTVPSKGELSREICLQSQSKDKSTTPGGTGIKPFLERFGERC
                     QEHSKESPARSTPHRTPIITPNTKAIQEKLFKQDTSSSTTHLAQQLKQERQKELACLRG
                     RFDKGNIWSAEKGGNSKSKQLETKQETHCQSTPLKKHQGVSKTQSLPVTEKVTENQIPA
                     KNSSTEPKEVIREIEMSVDDDDINSSKVINDLFSDVLEEGELDMEKSQEEMDQALAESS
                     EEQEDALNISSMSLLAPLAQTVGVVSPESLVSTPRLELKDTSRSDESPKPGKFQRTRVP
                     RAESGDSLGSEDRDLLYSIDAYRSQRFKETERPSIKQVIVRKEDVTSKLDEKNNAFPCQ
                     VNIKQKMQELNNEINMQQTVIYQASQALNCCVDEEHGKGSLEEAEAERLLLIATGKRTL
                     LIDELNKLKNEGPQRKNKASPQSEFMPSKGSVTLSEIRLPLKADFVCSTVQKPDAANYY
                     YLIILKAGAENMVATPLASTSNSLNGDALTFTTTFTLQDVSNDFEINIEVYSLVQKKDP
                     SGLDKKKKTSKSKAITPKRLLTSITTKSNIHSSVMASPGGLSAVRTSNFALVGSYTLSL
                     SSVGNTKFVLDKVPFLSSLEGHIYLKIKCQVNSSVEERGFLTIFEDVSGFGAWHRRWCV
                     LSGNCISYWTYPDDEKRKNPIGRINLANCTSRQIEPANREFCARRNTFELITVRPQRED
                     DRETLVSQCRDTLCVTKNWLSADTKEERDLWMQKLNQVLVDIRLWQPDACYKPIGKP"
     protein_bind    7412..7445
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    7867..8091
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7972..7983)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8094..8110)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8620..8800
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8862..9450)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9621..10481)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10482..10586)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga
     1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2581 cagcagagat ccagtttggt tagtaccgcg ctctagagat ccgacgccgc catctctagg
     2641 cccgcgccgg ccccctcgca cagacttgtg ggagaagctc ggctactccc ctgccccggt
     2701 taatttgcat ataatatttc ctagtaacta tagaggctta atgtgcgata aaagacagat
     2761 aatctgttct ttttaatact agctacattt tacatgatag gcttggattt ctataagaga
     2821 tacaaatact aaattattat tttaaaaaac agcacaaaag gaaactcacc ctaactgtaa
     2881 agtaattgtg tgttttgaga ctataaatat cccttggaga aaagccttgt ttcggcgatg
     2941 cctctttgaa taaattcaag agatttattc aaagaggcat cgccattttt tctcgaggtc
     3001 gacggtatcg ataagctcgc ttcacgagat tccagcaggt cgagggacct aataacttcg
     3061 tatagcatac attatacgaa gttatattaa gggttccaag cttaagcggc cgcatagtaa
     3121 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     3181 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     3241 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     3301 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     3361 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     3421 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     3481 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     3541 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     3601 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     3661 ataagcagag ctggtttagt gaaccgtcag atccgctagc gccaccatgg tgagcaaggg
     3721 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     3781 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     3841 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     3901 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     3961 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     4021 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     4081 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     4141 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     4201 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     4261 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     4321 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     4381 ccactccacc ggcggcatgg acgagctgta caagccgcgg atggatccgt ttacggagaa
     4441 actgctggag cgaacccgtg ccaggcgaga gaatcttcag agaaaaatgg ctgagaggcc
     4501 cacagcagct ccaaggtcta tgactcatgc taagcgagct agacagccac tttcagaagc
     4561 aagtaaccag cagcccctct ctggtggtga agagaaatct tgtacaaaac catcgccatc
     4621 aaaaaaacgc tgttctgaca acactgaagt agaagtttct aacttggaaa ataaacaacc
     4681 agttgagtcg acatctgcaa aatcttgttc tccaagtcct gtgtctcctc aggtgcagcc
     4741 acaagcagca gataccatca gtgattctgt tgctgtcccg gcatcactgc tgggcatgag
     4801 gagagggctg aactcaagat tggaagcaac tgcagcctcc tcagttaaaa cacgtatgca
     4861 aaaacttgca agcagtgtta agcaggaagc tacattctgt tcccaaaggg atggcgacgc
     4921 aagcttaaac aaggccctat cctcaagtgc tgctgatgcg tctttggtta atgcctcaat
     4981 ttccagctct gtgaaagcta cttctccagt gaaatctact acatctatca ctgatgctaa
     5041 aagttgtgag ggacaaaatc ctgagctact tccaaaaact cctattagtc ctctgaaaac
     5101 gggggtatcg aaaccaattg tgaagtcaac tttatcccag acagttccat ccaagggaga
     5161 attaagtaga gaaatttgtc tgcaatctca atctaaagac aaatctacga caccaggagg
     5221 aacaggaatt aagcctttcc tggaacgctt tggagagcgt tgtcaagaac atagcaaaga
     5281 aagtccagct cgtagcacac cccacagaac ccccattatt actccaaata caaaggccat
     5341 ccaagaaaaa ttattcaagc aagacacatc ttcatctact acccatttag cacaacagct
     5401 caagcaggaa cgtcaaaaag aactagcatg tcttcgtggc cgatttgaca agggcaatat
     5461 atggagtgca gaaaaaggcg gaaactcaaa aagcaaacaa ctagaaacca aacaggaaac
     5521 tcactgtcag agcactcccc tcaaaaaaca ccaaggtgtt tcaaaaactc agtcacttcc
     5581 agtaacagaa aaggtgaccg aaaaccagat accagccaaa aattctagta cagaacctaa
     5641 agaagtgata cgtgaaattg agatgagtgt ggatgatgat gatatcaata gttcgaaagt
     5701 aattaatgac ctcttcagtg atgtcctaga ggaaggtgaa ctagatatgg agaagagcca
     5761 agaggagatg gatcaagcat tagcagaaag cagcgaagaa caggaagatg cactgaatat
     5821 ctcctcaatg tctttacttg caccattggc acaaacagtt ggtgtggtaa gtccagagag
     5881 tttagtgtcc acacctagac tggaattgaa agacaccagc agaagtgatg aaagtccaaa
     5941 accaggaaaa ttccaaagaa ctcgtgtccc tcgagctgaa tctggtgata gccttggttc
     6001 tgaagatcgt gatcttcttt acagcattga tgcatataga tctcaaagat tcaaagaaac
     6061 agaacgtcca tcaataaagc aggtgattgt tcggaaggaa gatgttactt caaaactgga
     6121 tgaaaaaaat aatgcctttc cttgtcaagt taatatcaaa cagaaaatgc aggaactcaa
     6181 taacgaaata aatatgcaac agacagtgat ctatcaagct agccaggctc ttaactgctg
     6241 tgttgatgaa gaacatggaa aagggtccct agaagaagct gaagcagaaa gacttcttct
     6301 aattgcaact gggaagagaa cacttttgat tgatgaattg aataaattga agaacgaagg
     6361 acctcagagg aagaataagg ctagtcccca aagtgaattt atgccatcca aaggatcagt
     6421 tactttgtca gaaatccgct tgcctctaaa agcagatttt gtctgcagta cggttcagaa
     6481 accagatgca gcaaattact attacttaat tatactaaaa gcaggagctg aaaatatggt
     6541 agccacacca ttagcaagta cttcaaactc tcttaacggt gatgctctga cattcactac
     6601 tacatttact ctgcaagatg tatccaatga ctttgaaata aatattgaag tttacagctt
     6661 ggtgcaaaag aaagatccct caggccttga taagaagaaa aaaacatcca agtccaaggc
     6721 tattactcca aagcgactcc tcacatctat aaccacaaaa agcaacattc attcttcagt
     6781 catggccagt ccaggaggtc ttagtgctgt gcgaaccagc aacttcgccc ttgttggatc
     6841 ttacacatta tcattgtctt cagtaggaaa tactaagttt gttctggaca aggtcccctt
     6901 tttatcttct ttggaaggtc atatttattt aaaaataaaa tgtcaagtga attccagtgt
     6961 tgaagaaaga ggttttctaa ccatatttga agatgttagt ggttttggtg cctggcatcg
     7021 aagatggtgt gttctttctg gaaactgtat atcttattgg acttatccag atgatgagaa
     7081 acgcaagaat cccataggaa ggataaatct ggctaattgt accagtcgtc agatagaacc
     7141 agccaacaga gaattttgtg caagacgcaa cacttttgaa ttaattactg tccgaccaca
     7201 aagagaagat gaccgagaga ctcttgtcag ccaatgcagg gacacactct gtgttaccaa
     7261 gaactggctg tctgcagata ctaaagaaga gcgggatctc tggatgcaaa aactcaatca
     7321 agttcttgtt gatattcgcc tctggcaacc tgatgcttgc tacaaaccta ttggaaagcc
     7381 ttaacctgca ggcaattcgt cgagggacct aataacttcg tatagcatac attatacgaa
     7441 gttatacatg tttaagggtt ccggttccac taggtacaat tcgatatcaa gcttatcgat
     7501 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     7561 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7621 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7681 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7741 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7801 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7861 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     7921 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7981 aatccagcgg accttccttc ccgcgcggcc tgctgccggc tctgcggcct cttccgcgtc
     8041 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     8101 gtcgacctcg atcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     8161 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     8221 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     8281 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     8341 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     8401 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     8461 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
     8521 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
     8581 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
     8641 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     8701 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     8761 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatgtgagc aaaaggccag
     8821 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8881 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8941 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     9001 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     9061 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     9121 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     9181 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     9241 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9301 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9361 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9421 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9481 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9541 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9601 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9661 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9721 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9781 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9841 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9901 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9961 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    10021 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    10081 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    10141 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    10201 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    10261 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    10321 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    10381 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    10441 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    10501 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    10561 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
