LOCUS       Exported               10404 bp DNA     circular SYN 08-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Institute for Molecular Bioscience
  TITLE     Direct Submission
  JOURNAL   Exported Jun 7, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    772..792
                     /label=cmv-f
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1686..1919
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2446..2563
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2618..2888
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     misc_feature    2932..2991
                     /label=Anillin shRNA
     misc_feature    2955..2963
                     /label=shRNA loop
     primer_bind     2994..3010
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3052..3085
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     enhancer        3165..3468
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3469..3672
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3701..3710
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3707..4414
                     /codon_start=1
                     /label=mCherry
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             4421..7174
                     /codon_start=1
                     /label=Anillin-Delta-Act
                     /translation="MDPFTEKLLERTRARRENLQRKMAERPTAAPRSMTHAKRARQPLS
                     EASNQQPLSGGEEKSCTKPSPSKKRCSDNTEVEVSNLENKQPVESTSAKSCSPSPVSPQ
                     VQPQAADTISDSVAVPASLLGMRRGLNSRLEATAASSVKTRMQKLAEQRRRWDNDDMTD
                     DIPESSLFSPMPSEEKAASPPRPLLSNASATPVGRRGRLANLAATICSWEDDVNHSFAK
                     QNSVQEQPGTACLSKFSSASGASARINSTHLAQQLKQERQKELACLRGRFDKGNIWSAE
                     KGGNSKSKQLETKQETHCQSTPLKKHQGVSKTQSLPVTEKVTENQIPAKNSSTEPKEVI
                     REIEMSVDDDDINSSKVINDLFSDVLEEGELDMEKSQEEMDQALAESSEEQEDALNISS
                     MSLLAPLAQTVGVVSPESLVSTPRLELKDTSRSDESPKPGKFQRTRVPRAESGDSLGSE
                     DRDLLYSIDAYRSQRFKETERPSIKQVIVRKEDVTSKLDEKNNAFPCQVNIKQKMQELN
                     NEINMQQTVIYQASQALNCCVDEEHGKGSLEEAEAERLLLIATGKRTLLIDELNKLKNE
                     GPQRKNKASPQSEFMPSKGSVTLSEIRLPLKADFVCSTVQKPDAANYYYLIILKAGAEN
                     MVATPLASTSNSLNGDALTFTTTFTLQDVSNDFEINIEVYSLVQKKDPSGLDKKKKTSK
                     SKAITPKRLLTSITTKSNIHSSVMASPGGLSAVRTSNFALVGSYTLSLSSVGNTKFVLD
                     KVPFLSSLEGHIYLKIKCQVNSSVEERGFLTIFEDVSGFGAWHRRWCVLSGNCISYWTY
                     PDDEKRKNPIGRINLANCTSRQIEPANREFCARRNTFELITVRPQREDDRETLVSQCRD
                     TLCVTKNWLSADTKEERDLWMQKLNQVLVDIRLWQPDACYKPIGKP"
     protein_bind    7202..7235
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    7657..7881
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7762..7773)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7884..7900)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8410..8590
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8652..9240)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9411..10271)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10272..10376)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga
     1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2581 cagcagagat ccagtttggt tagtaccgcg ctctagagat ccgacgccgc catctctagg
     2641 cccgcgccgg ccccctcgca cagacttgtg ggagaagctc ggctactccc ctgccccggt
     2701 taatttgcat ataatatttc ctagtaacta tagaggctta atgtgcgata aaagacagat
     2761 aatctgttct ttttaatact agctacattt tacatgatag gcttggattt ctataagaga
     2821 tacaaatact aaattattat tttaaaaaac agcacaaaag gaaactcacc ctaactgtaa
     2881 agtaattgtg tgttttgaga ctataaatat cccttggaga aaagccttgt ttcggcgatg
     2941 cctctttgaa taaattcaag agatttattc aaagaggcat cgccattttt tctcgaggtc
     3001 gacggtatcg ataagctcgc ttcacgagat tccagcaggt cgagggacct aataacttcg
     3061 tatagcatac attatacgaa gttatattaa gggttccaag cttaagcggc cgcatagtaa
     3121 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     3181 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     3241 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     3301 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     3361 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     3421 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     3481 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     3541 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     3601 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     3661 ataagcagag ctggtttagt gaaccgtcag atccgctagc gccaccatgg tgagcaaggg
     3721 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     3781 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     3841 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     3901 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     3961 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     4021 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     4081 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     4141 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     4201 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     4261 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     4321 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     4381 ccactccacc ggcggcatgg acgagctgta caagccgcgg atggatccgt ttacggagaa
     4441 actgctggag cgaacccgtg ccaggcgaga gaatcttcag agaaaaatgg ctgagaggcc
     4501 cacagcagct ccaaggtcta tgactcatgc taagcgagct agacagccac tttcagaagc
     4561 aagtaaccag cagcccctct ctggtggtga agagaaatct tgtacaaaac catcgccatc
     4621 aaaaaaacgc tgttctgaca acactgaagt agaagtttct aacttggaaa ataaacaacc
     4681 agttgagtcg acatctgcaa aatcttgttc tccaagtcct gtgtctcctc aggtgcagcc
     4741 acaagcagca gataccatca gtgattctgt tgctgtcccg gcatcactgc tgggcatgag
     4801 gagagggctg aactcaagat tggaagcaac tgcagcctcc tcagttaaaa cacgtatgca
     4861 aaaacttgca gagcaacggc gccgttggga taatgatgat atgacagatg acattcctga
     4921 aagctcactc ttctcaccaa tgccatcaga ggaaaaggct gcttcccctc ccagacctct
     4981 gctttcaaat gcctcggcaa ctccagttgg cagaaggggc cgtctggcca atcttgctgc
     5041 aactatttgc tcctgggaag atgatgtaaa tcactcattt gcaaaacaaa acagtgtaca
     5101 agaacagcct ggtaccgctt gtttatccaa attttcctct gcaagtggag catctgctag
     5161 gatcaatagc acccatttag cacaacagct caagcaggaa cgtcaaaaag aactagcatg
     5221 tcttcgtggc cgatttgaca agggcaatat atggagtgca gaaaaaggcg gaaactcaaa
     5281 aagcaaacaa ctagaaacca aacaggaaac tcactgtcag agcactcccc tcaaaaaaca
     5341 ccaaggtgtt tcaaaaactc agtcacttcc agtaacagaa aaggtgaccg aaaaccagat
     5401 accagccaaa aattctagta cagaacctaa agaagtgata cgtgaaattg agatgagtgt
     5461 ggatgatgat gatatcaata gttcgaaagt aattaatgac ctcttcagtg atgtcctaga
     5521 ggaaggtgaa ctagatatgg agaagagcca agaggagatg gatcaagcat tagcagaaag
     5581 cagcgaagaa caggaagatg cactgaatat ctcctcaatg tctttacttg caccattggc
     5641 acaaacagtt ggtgtggtaa gtccagagag tttagtgtcc acacctagac tggaattgaa
     5701 agacaccagc agaagtgatg aaagtccaaa accaggaaaa ttccaaagaa ctcgtgtccc
     5761 tcgagctgaa tctggtgata gccttggttc tgaagatcgt gatcttcttt acagcattga
     5821 tgcatataga tctcaaagat tcaaagaaac agaacgtcca tcaataaagc aggtgattgt
     5881 tcggaaggaa gatgttactt caaaactgga tgaaaaaaat aatgcctttc cttgtcaagt
     5941 taatatcaaa cagaaaatgc aggaactcaa taacgaaata aatatgcaac agacagtgat
     6001 ctatcaagct agccaggctc ttaactgctg tgttgatgaa gaacatggaa aagggtccct
     6061 agaagaagct gaagcagaaa gacttcttct aattgcaact gggaagagaa cacttttgat
     6121 tgatgaattg aataaattga agaacgaagg acctcagagg aagaataagg ctagtcccca
     6181 aagtgaattt atgccatcca aaggatcagt tactttgtca gaaatccgct tgcctctaaa
     6241 agcagatttt gtctgcagta cggttcagaa accagatgca gcaaattact attacttaat
     6301 tatactaaaa gcaggagctg aaaatatggt agccacacca ttagcaagta cttcaaactc
     6361 tcttaacggt gatgctctga cattcactac tacatttact ctgcaagatg tatccaatga
     6421 ctttgaaata aatattgaag tttacagctt ggtgcaaaag aaagatccct caggccttga
     6481 taagaagaaa aaaacatcca agtccaaggc tattactcca aagcgactcc tcacatctat
     6541 aaccacaaaa agcaacattc attcttcagt catggccagt ccaggaggtc ttagtgctgt
     6601 gcgaaccagc aacttcgccc ttgttggatc ttacacatta tcattgtctt cagtaggaaa
     6661 tactaagttt gttctggaca aggtcccctt tttatcttct ttggaaggtc atatttattt
     6721 aaaaataaaa tgtcaagtga attccagtgt tgaagaaaga ggttttctaa ccatatttga
     6781 agatgttagt ggttttggtg cctggcatcg aagatggtgt gttctttctg gaaactgtat
     6841 atcttattgg acttatccag atgatgagaa acgcaagaat cccataggaa ggataaatct
     6901 ggctaattgt accagtcgtc agatagaacc agccaacaga gaattttgtg caagacgcaa
     6961 cacttttgaa ttaattactg tccgaccaca aagagaagat gaccgagaga ctcttgtcag
     7021 ccaatgcagg gacacactct gtgttaccaa gaactggctg tctgcagata ctaaagaaga
     7081 gcgggatctc tggatgcaaa aactcaatca agttcttgtt gatattcgcc tctggcaacc
     7141 tgatgcttgc tacaaaccta ttggaaagcc ttaacctgca ggcaattcgt cgagggacct
     7201 aataacttcg tatagcatac attatacgaa gttatacatg tttaagggtt ccggttccac
     7261 taggtacaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     7321 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7381 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7441 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7501 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     7561 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     7621 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7681 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7741 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcgcggcc
     7801 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7861 ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct agaaaaacat
     7921 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     7981 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     8041 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     8101 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     8161 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
     8221 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
     8281 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
     8341 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
     8401 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     8461 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8521 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8581 atctctagca gcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8641 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8701 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8761 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8821 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8881 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8941 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9001 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9061 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     9121 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9181 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9241 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9301 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9361 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9421 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9481 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9541 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9601 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9661 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9721 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9781 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9841 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9901 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9961 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10021 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10081 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10141 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10201 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10261 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10321 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10381 tttccccgaa aagtgccacc tgac
//
