LOCUS       pJT309_RapInducible1-Ac 4573 bp ds-DNA     circular     07-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     protein_bind    17..28
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_feature    17..46
                     /label="extra sites for pos feedback"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    29..34
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    35..46
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_feature    53..132
                     /label="pSCP1 I think"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     source          53..138
                     /label="source"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    142..147
                     /label="Kozak sequence"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             148..1269
                     /label="Translation 148-1269"
     CDS             151..177
                     /label="c-myc NLS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             178..441
                     /label="FRB"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             178..441
                     /label="Translation 178-441"
     primer          444..462
                     /label="HTrec F bind"
                     /note="sequence: tggATCCggaTCAggctcc"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    445..462
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    446..462
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    463..483
                     /label="TypeIIS cloning site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             463..483
                     /label="Translation 463-483"
     CDS             463..483
                     /label="Translation 463-483"
     CDS             484..489
                     /label="Translation 484-489"
     misc_feature    484..492
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(484..503)
                     /label="HTrec R bind site"
                     /note="sequence: cccctTccctcTCcagaTcc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             484..546
                     /label="Translation 484-546"
     CDS             484..546
                     /label="Translation 484-546"
     CDS             484..546
                     /label="Translation 484-546"
     CDS             490..492
                     /label="Translation 490-492"
     misc_feature    493..546
                     /label="T2A"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             493..546
                     /label="Translation 493-546"
     primer          complement(528..546)
                     /label="JT809"
                     /note="sequence: tgggccaggattctcctcg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             547..573
                     /label="c-myc NLS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             580..942
                     /label="ZFHD1 (DNA binding domain)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             949..1269
                     /label="FKBP"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             949..1272
                     /label="Translation 949-1272"
     source          1279..1295
                     /label="source"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1279..1295
                     /label="1x sNRP-1 term"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     protein_bind    1302..1313
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    1314..1319
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    1320..1331
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    1332..1337
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    1338..1349
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    1350..1353
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    1354..1355
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    1356..1367
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    1368..1373
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    1374..1385
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    1386..1391
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    1392..1403
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    1404..1409
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    1410..1421
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    1422..1427
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    1428..1439
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    1440..1445
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    1446..1457
                     /label="ZFHD1 binding sites"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        1474..1587
                     /label="minimal IL-2 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1591..1600
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1597..2313
                     /label="citrine"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1597..2313
                     /label="Translation 1597-2313"
     misc_feature    1628..1647
                     /label="MCB320 sgRNA site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1778..1797
                     /label="citrine sgRNA 2"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1974..1993
                     /label="cit3"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             2314..2316
                     /label="Translation 2314-2316"
     misc_feature    2317..2369
                     /label="min rabbit B globin UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     repeat_region   2386..2394
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     repeat_region   2386..2394
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     complement(2461..2481)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     4504..4521
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 TGATCGATGC acgcgtTAAT GATGGGCGCt acAGTAATGA TGGGCGGAAT TCctcgaggt
       61 acttatataa gggggtgggg gcgcgttcgt cctcagtcgc gatcgaacac tcgagccgag
      121 cagacgtgcc taaccggtgc cgccaccatg cctgctgcca agagggtcaa gttggactgg
      181 catgaaggcc tggaagaggc atctcgtttg tactttgggg aaaggaacgt gaaaggcatg
      241 tttgaggtgc tggagccctt gcatgctatg atggaacggg gcccccagac tctgaaggaa
      301 acatcTttta atcaggccta tggtcgTgat ttaatggagg cccaagagtg gtgcaggaag
      361 tacatgaaat cagggaatgt caaggacctc acccaagcct gggacctcta ttatcatgtg
      421 ttccgacgaa tctcaaagca gtctggATCC ggaTCAggct ccgGAGACGG GtcaAgCGTC
      481 TCtggAtctg GAgagggAag gggAagcctc ctaacttgcg gagaTgtcga ggagaatcct
      541 ggcccacctg cCgcTaagCG Tgtcaagttg gactctagag aacgcccGta tgcttgccct
      601 gtcgagtcct gcgatcgccg cttttctcgc tcggatgagc ttacccgcca tatccgcatc
      661 cacacaggcc agaagccctt ccagtgtcga atctgcatgc gtaacttcag tcgtagtgac
      721 caccttacca cccacatccg cacccacaca ggcggcggcc gcaggaggaa gaaacgcacc
      781 agcatagaga cTaacatccg tgtggcctta gagaagagtt tcttggagaa tcaaaagcct
      841 acctcggaag agatcactat gattgctgat cagctcaata tggaaaaaga ggtgattcgt
      901 gtttggttct gtaaccgccg ccagaaagaa aaaagaatca acaagggcgg cgtgcaggtg
      961 gagactatct ccccaggcga cgggcgcacc ttccccaagc gcggccagac ctgcgtggtg
     1021 cactacaccg ggatgcttga agatggaaag aaatttgatt cctcccggga cagaaacaag
     1081 ccctttaagt ttatgctagg caagcaggag gtgatccgag gctgggaaga aggggttgcc
     1141 cagatgagtg tgggtcagag agccaaactg actatatctc cagattatgc ctatggtgcc
     1201 actgggcacc caggcatcat cccaccacat gccactctcg tcttTgatgt ggagcttcta
     1261 aaactggaaT AAcatatgaa ataaaatacg aaatgCGTAC GTAATGATGG GCGCTaaatT
     1321 AATGATGGGC GgagttGTAA TGATGGGCGC tcgttTAATG ATGGGCGTCT AGCTAATGAT
     1381 GGGCGggCGA tTAATGATGG GCGaagGACT AATGATGGGC GCagcgGTAA TGATGGGCGa
     1441 tcAGcTAATG ATGGGCGACG CGAATTAATT CAACATTTTG ACACCCCCAT AATATTTTTC
     1501 CAGAATTAAC AGTATAAATT GCATCTCTTG TTCAAGAGTT CCCTATCACT CTCTTTAATC
     1561 ACTACTCACA GTAACCTCAA CTCCTGCgcc gccaccatgg tgagcaaggg cgaggagctg
     1621 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1681 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1741 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc
     1801 ctgatgtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1861 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1921 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1981 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     2041 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     2101 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     2161 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg
     2221 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     2281 gggatcactc tcggcatgga cgagctgtac aagTAAGCCT AATAAAGAGC TCAGATGCAT
     2341 GCATCAGAGT GTGTTGGTTT TTTGTGTGTc ggaccgagcg gccgcaggaa ccccATGATC
     2401 GGATTAGCAG GCTAGGTGGA GGCTCAGTGA TGATAAGTCT GCGATGGTGG ATGCATGTGT
     2461 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CAGAGGGCAC AATCCTATTC
     2521 CGCGCTATCC GACAATCTCC AAGACATTAG GTGGAGTTCA GTTCGGCGTA TGGCATATGT
     2581 CGCTGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC
     2641 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     2701 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     2761 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     2821 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     2881 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     2941 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     3001 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     3061 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     3121 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     3181 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     3241 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCTA TTCAACAAAG CCGCCGTCCC
     3301 GTCAAGTCAG CGTAAATGGG TAGGGGGCTT CAAATCGTCC GCTCTGCCAG TGTTACAACC
     3361 AATTAACAAA TTCTGATTAG AAAAACTCAT CGAGCATCAA ATGAAACTGC AATTTATTCA
     3421 TATCAGGATT ATCAATACCA TATTTTTGAA AAAGCCGTTT CTGTAATGAA GGAGAAAACT
     3481 CACCGAGGCA GTTCCATAGG ATGGCAAGAT CCTGGTATCG GTCTGCGATT CCGACTCGTC
     3541 CAACATCAAT ACAACCTATT AATTTCCCCT CGTCAAAAAT AAGGTTATCA AGTGAGAAAT
     3601 CACCATGAGT GACGACTGAA TCCGGTGAGA ATGGCAAAAG CTTATGCATT TCTTTCCAGA
     3661 CTTGTTCAAC AGGCCAGCCA TTACGCTCGT CATCAAAATC ACTCGCATCA ACCAAACCGT
     3721 TATTCATTCG TGATTGCGCC TGAGCGAGAC GAAATACGCG ATCGCTGTTA AAAGGACAAT
     3781 TACAAACAGG AATCGAATGC AACCGGCGCA GGAACACTGC CAGCGCATCA ACAATATTTT
     3841 CACCTGAATC AGGATATTCT TCTAATACCT GGAATGCTGT TTTCCCGGGG ATCGCAGTGG
     3901 TGAGTAACCA TGCATCATCA GGAGTACGGA TAAAATGCTT GATGGTCGGA AGAGGCATAA
     3961 ATTCCGTCAG CCAGTTTAGT CTGACCATCT CATCTGTAAC ATCATTGGCA ACGCTACCTT
     4021 TGCCATGTTT CAGAAACAAC TCTGGCGCAT CGGGCTTCCC ATACAATCGA TAGATTGTCG
     4081 CACCTGATTG CCCGACATTA TCGCGAGCCC ATTTATACCC ATATAAATCA GCATCCATGT
     4141 TGGAATTTAA TCGCGGCCTC GAGCAAGACG TTTCCCGTTG AATATGGCTC ATAACACCCC
     4201 TTGTATTACT GTTTATGTAA GCAGACAGTT TTATTGTTCA TGATGATATA TTTTTATCTT
     4261 GTGCAATGTA ACATCAGAGA TTTTGAGACA CAACGTGGCT TTCCCCCGCC GCTCTAGAAC
     4321 TAGTGGATCC AAATAAAACG AAAGGCTCAG TCGAAAGACT GGGCCTTTCG TTTTATCTGT
     4381 TGTTTGTCGC ATTATACGAG ACGTCCAGGT TGGGATACCT GAAACAAAAC CCATCGTACG
     4441 GCCAAGGAAG TCTCCAATAA CTGTGATCCA CCACAAGCGC CAGGGTTTTC CCAGTCACGA
     4501 CGTTGTAAAA CGACGGCCAG TCATGCATAA TCCGCACGCA TCTGGAATAA GGAAGTGCCA
     4561 TTCCGCCTGA CCT
//