LOCUS       pJT055_AAVS1-BSD-9xTet0 8417 bp ds-DNA     circular     24-JUL-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     protein_bind    819..840
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        855..860
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        861..878
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        879..885
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    893..909
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     primer_bind     917..933
                     /label="M13 rev"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     promoter        954..972
                     /label="T3 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1007..1810
                     /label="left_arm"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1817..1842
                     /label="SA"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             1866..1919
                     /label="T2A"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1866..1928
                     /label="Translation 1866-1928"
     CDS             1929..2327
                     /label="BSD"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             1929..2327
                     /label="Translation 1929-2327"
     misc_feature    2365..2589
                     /label="polyA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(2434..2453)
                     /label="QB3668"
                     /note="sequence: ggaaaggacagtgggagtgg"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2644..2967
                     /label="9xTRE"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2661..2679
                     /label="TRE"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2697..2715
                     /label="TRE"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2733..2751
                     /label="TRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2769..2787
                     /label="TRE"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2805..2823
                     /label="TRE"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2841..2859
                     /label="TRE"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2877..2895
                     /label="TRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2913..2931
                     /label="TRE"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2949..2967
                     /label="TRE"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        3005..4159
                     /label="pEF"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4232..4237
                     /label="Kozak sequence"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             4238..4300
                     /label="Igκ leader"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    4288..4307
                     /label="sgRNA_Sp"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    4294..4313
                     /label="sgRNA_Sa"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             4340..5020
                     /label="hIgG1-Fc"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             5021..5068
                     /label="Linker"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             5069..5098
                     /label="Myc"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             5099..5248
                     /label="PDGFRB Transmembrane Domain"
                     /ApEinfo_revcolor=#3366ff
                     /ApEinfo_fwdcolor=#3366ff
     CDS             5264..5317
                     /label="T2A"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5324..5329
                     /label="Kozak sequence"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             5330..5332
                     /label="Citrine"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             5330..6067
                     /label="Translation 5330-6067"
     CDS             5333..5335
                     /label="Citrine"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             5336..6046
                     /label="Citrine"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             6047..6067
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             6068..6070
                     /label="Translation 6068-6070"
     misc_feature    6073..6076
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    6077..6080
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     polyA_signal    6078..6199
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    6081..6081
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    6082..6082
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     misc_feature    6083..6208
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    6242..6249
                     /label="Insertion from Gibson assembly"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    6265..7101
                     /label="right_arm"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(7376..8167)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(8168..8236)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     rep_origin      join(8360..8417,1..531)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
       61 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
      121 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
      181 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
      241 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
      301 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
      361 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      421 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      481 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      541 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      601 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
      661 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
      721 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
      781 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
      841 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      901 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct cagaattaac
      961 cctcactaaa gggactagtc ctgcaggttt aaacgaattc gccctttgct ttctctgacc
     1021 agcattctct cccctgggcc tgtgccgctt tctgtctgca gcttgtggcc tgggtcacct
     1081 ctacggctgg cccagatcct tccctgccgc ctccttcagg ttccgtcttc ctccactccc
     1141 tcttcccctt gctctctgct gtgttgctgc ccaaggatgc tctttccgga gcacttcctt
     1201 ctcggcgctg caccacgtga tgtcctctga gcggatcctc cccgtgtctg ggtcctctcc
     1261 gggcatctct cctccctcac ccaaccccat gccgtcttca ctcgctgggt tcccttttcc
     1321 ttctccttct ggggcctgtg ccatctctcg tttcttagga tggccttctc cgacggatgt
     1381 ctcccttgcg tcccgcctcc ccttcttgta ggcctgcatc atcaccgttt ttctggacaa
     1441 ccccaaagta ccccgtctcc ctggctttag ccacctctcc atcctcttgc tttctttgcc
     1501 tggacacccc gttctcctgt ggattcgggt cacctctcac tcctttcatt tgggcagctc
     1561 ccctaccccc cttacctctc tagtctgtgc tagctcttcc agccccctgt catggcatct
     1621 tccaggggtc cgagagctca gctagtcttc ttcctccaac ccgggcccct atgtccactt
     1681 caggacagca tgtttgctgc ctccagggat cctgtgtccc cgagctggga ccaccttata
     1741 ttcccagggc cggttaatgt ggctctggtt ctgggtactt ttatctgtcc cctccacccc
     1801 acagtggggc aagcttctga cctcttctct tcctcccaca gggcctcgag agatctggca
     1861 gcggagaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta
     1921 ggctcgagat ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
     1981 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta
     2041 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
     2101 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
     2161 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc
     2221 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag
     2281 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaatct agagggcccg
     2341 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     2401 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     2461 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     2521 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     2581 gctctatggc ttctgaggcg gaagaaccag ctgggggcta gcaaagccta ggaacagctg
     2641 catatagcac tgcagtttac tccctatcag tgatagagag tcaaaaggcc atgccctccc
     2701 tatcagtgat agagagagca gactcaaggt ggtccctatc agtgatagag agattgagca
     2761 agagggcctc cctatcagtg atagagaata gcactgcagt ttactcccta tcagtgatag
     2821 agagtcaaaa ggccatgccc tccctatcag tgatagagag agcagactca aggtggtccc
     2881 tatcagtgat agagagattg agcaagaggg cctccctatc agtgatagag attgatccag
     2941 tcagggcctc cctatcagtg atagagaagc tatgtgcagg tagggctgta cagtgggaat
     3001 gcatgagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     3061 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     3121 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     3181 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     3241 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     3301 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     3361 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     3421 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     3481 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     3541 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     3601 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     3661 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     3721 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     3781 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     3841 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     3901 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     3961 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     4021 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     4081 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     4141 tccatttcag gtgtcgtgag ctagcgatta cgccaagctc gaaattaacc ctcactaaag
     4201 ggaacaaaag ctggagctcc caccgcggtg ggccaccATG GAGACAGACA CACTCCTGCT
     4261 ATGGGTACTG CTGCTCTGGG TTCCAGGTTC CACTGGTGAC GCTAGCGAGA CGGGCGGATC
     4321 AGGCGGTCGT CTCGGATCCG ACAAAACTCA CACATGCCCA CCGTGCCCAG CACCTGAACT
     4381 CCTGGGGGGA CCGTCAGTCT TCCTCTTCCC CCCAAAACCC AAGGACACCC TCATGATCTC
     4441 CCGGACCCCT GAGGTCACAT GCGTGGTGGT GGACGTGAGC CACGAAGACC CTGAGGTCAA
     4501 GTTCAACTGG TACGTGGACG GCGTGGAGGT GCATAATGCC AAGACAAAGC CGCGGGAGGA
     4561 GCAGTACAAC AGCACGTACC GTGTGGTCAG CGTCCTCACC GTCCTGCACC AGGACTGGCT
     4621 GAATGGCAAG GAGTACAAGT GCAAGGTCTC CAACAAAGCC CTCCCAGCCC CCATCGAGAA
     4681 AACCATCTCC AAAGCCAAAG GGCAGCCCCG AGAACCACAG GTCTACACCC TGCCCCCATC
     4741 CCGGGAGGAG ATGACCAAGA ACCAGGTCAG CCTGACCTGC CTGGTCAAAG GCTTCTATCC
     4801 CAGCGACATC GCCGTGGAGT GGGAGAGCAA TGGGCAGCCG GAGAACAACT ACAAGACCAC
     4861 GCCTCCCGTG CTGGACTCCG ACGGCTCCTT CTTCCTCTAC AGCAAGCTCA CCGTGGACAA
     4921 GAGCAGGTGG CAGCAGGGGA ACGTCTTCTC ATGCTCCGTG ATGCACGAGG CTCTGCACAA
     4981 CCACTACACG CAGAAGAGCC TCTCCCTGTC TCCGGGTAAA GGAGGAGGTG GAAGCGGAGG
     5041 AGGAGGAAGC GGAGGAGGAG GTAGCggaGA ACAAAAACTC ATCTCAGAAG AGGATCTGAA
     5101 TGCTGTGGGC CAGGACACGC AGGAGGTCAT CGTGGTGCCA CACTCCTTGC CCTTTAAGGT
     5161 GGTGGTGATC TCAGCCATCC TGGCCCTGGT GGTGCTCACC ATCATCTCCC TTATCATCCT
     5221 CATCATGCTT TGGCAGAAGA AGCCACGTtg tacaGGCAGT GGAGAGGGCA GAGGAAGTCT
     5281 GCTAACATGC GGTGACGTCG AGGAGAATCC TGGCCCAccg gtcgccacca tggtgagcaa
     5341 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     5401 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     5461 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     5521 cttcggctac ggcctgatgt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     5581 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     5641 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     5701 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     5761 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     5821 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     5881 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta
     5941 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     6001 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagCCAA AAAAGAAGAG
     6061 AAAGGTAtag ttaactaaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6121 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6181 aactcatcaa tgtatcttat catgtctgCA tatgtaaatt gtaagcgtct cggcctctag
     6241 aGTCGAGATg acattgatta ttgaactagg gacaggattg gtgacagaaa agccccatcc
     6301 ttaggcctcc tccttcctag tctcctgata ttgggtctaa cccccacctc ctgttaggca
     6361 gattccttat ctggtgacac acccccattt cctggagcca tctctctcct tgccagaacc
     6421 tctaaggttt gcttacgatg gagccagaga ggatcctggg agggagagct tggcaggggg
     6481 tgggagggaa gggggggatg cgtgacctgc ccggttctca gtggccaccc tgcgctaccc
     6541 tctcccagaa cctgagctgc tctgacgcgg ctgtctggtg cgtttcactg atcctggtgc
     6601 tgcagcttcc ttacacttcc caagaggaga agcagtttgg aaaaacaaaa tcagaataag
     6661 ttggtcctga gttctaactt tggctcttca cctttctagt ccccaattta tattgttcct
     6721 ccgtgcgtca gttttacctg tgagataagg ccagtagcca gccccgtcct ggcagggctg
     6781 tggtgaggag gggggtgtcc gtgtggaaaa ctccctttgt gagaatggtg cgtcctaggt
     6841 gttcaccagg tcgtggccgc ctctactccc tttctctttc tccatccttc tttccttaaa
     6901 gagtccccag tgctatctgg gacatattcc tccgcccaga gcagggtccc gcttccctaa
     6961 ggccctgctc tgggcttctg ggtttgagtc cttggcaagc ccaggagagg cgctcaggct
     7021 tccctgtccc ccttcctcgt ccaccatctc atgcccctgg ctctcctgcc ccttccctac
     7081 aggggttcct ggctctgctc ttcgccttct tgacgagttc ttctgaatta ttaacgctta
     7141 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatcag
     7201 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     7261 caaatatgta tccgctcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7321 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7381 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7441 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7501 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7561 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7621 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7681 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7741 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7801 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7861 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7921 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7981 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8041 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8101 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8161 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8221 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8281 gtctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     8341 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8401 caaaaaaacc accgcta
//