LOCUS       pJT241_Lenti_pEF-dCas9 14997 bp ds-DNA     circular     07-JUN-2022
DEFINITION  .
KEYWORDS    "marker:BleoR, AmpR"                                               
FEATURES             Location/Qualifiers
     enhancer        238..617
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        619..817
                     /label="CMV promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     LTR             835..1015
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1062..1187
                     /label="HIV-1 Ψ"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1680..1913
                     /label="RRE"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2440..2557
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        2687..3865
                     /label="EF-1α promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     intron          2918..3856
                     /label="EF-1α intron A"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     CDS             3881..3928
                     /label="nucleoplasmin NLS"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             3929..8029
                     /label="dCas9"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             3929..9664
                     /label="Translation 3929-9664"
     primer          5530..5547
                     /label="QB5513"
                     /note="sequence: AAAGCCCGCCTTCCTGAG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          6167..6185
                     /label="JT-890"
                     /note="sequence: GTGAAAGTGATGGGCCGGC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          6792..6811
                     /label="JT-891"
                     /note="sequence: TCACCCTGAAGTCCAAGCTG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          7342..7361
                     /label="JT-892"
                     /note="sequence: GGCCTATTCTGTGCTGGTGG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             8084..8104
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    8120..8185
                     /label="3XFLAG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             8120..8206
                     /label="Translation 8120-8206"
     primer          8188..8206
                     /label="HTrec F bind"
                     /note="sequence: tggATCCggaTCAggctcc"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    8189..8206
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    8207..8419
                     /label="krabDMS089"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             8420..8425
                     /label="Translation 8420-8425"
     misc_feature    8420..8428
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(8420..8439)
                     /label="HTrec R bind site"
                     /note="sequence: cccctTccctcTCcagaTcc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             8426..8428
                     /label="Translation 8426-8428"
     misc_feature    8429..8482
                     /label="T2A"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             8429..8482
                     /label="Translation 8429-8482"
     primer          complement(8464..8482)
                     /label="JT809"
                     /note="sequence: tgggccaggattctcctcg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    8483..9193
                     /label="mTagBFP2 (benchling human codon opt)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             8483..9193
                     /label="Translation 8483-9193"
     primer          8839..8859
                     /label="QB2350"
                     /note="sequence: GATCTACAACGTCAAGATCAG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    9200..9265
                     /label="P2A"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             9200..9265
                     /label="Translation 9200-9265"
     CDS             9266..9664
                     /label="BSD"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    9689..10277
                     /label="WPRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(10160..10171)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     complement(10280..10296)
                     /label="KS primer"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     LTR             10802..10982
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     polyA_signal    11014..11238
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      11284..11712
                     /label="f1 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        11726..12055
                     /label="SV40 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      11906..12041
                     /label="SV40 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        12103..12150
                     /label="EM7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             12169..12543
                     /label="BleoR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     polyA_signal    12673..12794
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(12843..12859)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     protein_bind    12867..12883
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        complement(12891..12897)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(12898..12915)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(12916..12921)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    12936..12957
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     rep_origin      complement(13245..13833)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(14004..14795)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(14796..14864)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        complement(14865..14969)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagcTGCA AAGATGGATA AAGTTTTAAA CAGAGAGGAA
     2641 TCTTTGCAGC TAATGGACCT TCTAGGTCTT GAAAGGAGTG GGAATTGGCT CCGGTGCCCG
     2701 TCAGTGGGCA GAGCGCACAT CGCCCACAGT CCCCGAGAAG TTGGGGGGAG GGGTCGGCAA
     2761 TTGAACCGGT GCCTAGAGAA GGTGGCGCGG GGTAAACTGG GAAAGTGATG TCGTGTACTG
     2821 GCTCCGCCTT TTTCCCGAGG GTGGGGGAGA ACCGTATATA AGTGCAGTAG TCGCCGTGAA
     2881 CGTTCTTTTT CGCAACGGGT TTGCCGCCAG AACACAGGTA AGTGCCGTGT GTGGTTCCCG
     2941 CGGGCCTGGC CTCTTTACGG GTTATGGCCC TTGCGTGCCT TGAATTACTT CCACCTGGCT
     3001 GCAGTACGTG ATTCTTGATC CCGAGCTTCG GGTTGGAAGT GGGTGGGAGA GTTCGAGGCC
     3061 TTGCGCTTAA GGAGCCCCTT CGCCTCGTGC TTGAGTTGAG GCCTGGCCTG GGCGCTGGGG
     3121 CCGCCGCGTG CGAATCTGGT GGCACCTTCG CGCCTGTCTC GCTGCTTTCG ATAAGTCTCT
     3181 AGCCATTTAA AATTTTTGAT GACCTGCTGC GACGCTTTTT TTCTGGCAAG ATAGTCTTGT
     3241 AAATGCGGGC CAAGATCTGC ACACTGGTAT TTCGGTTTTT GGGGCCGCGG GCGGCGACGG
     3301 GGCCCGTGCG TCCCAGCGCA CATGTTCGGC GAGGCGGGGC CTGCGAGCGC GGCCACCGAG
     3361 AATCGGACGG GGGTAGTCTC AAGCTGGCCG GCCTGCTCTG GTGCCTGGCC TCGCGCCGCC
     3421 GTGTATCGCC CCGCCCTGGG CGGCAAGGCT GGCCCGGTCG GCACCAGTTG CGTGAGCGGA
     3481 AAGATGGCCG CTTCCCGGCC CTGCTGCAGG GAGCTCAAAA TGGAGGACGC GGCGCTCGGG
     3541 AGAGCGGGCG GGTGAGTCAC CCACACAAAG GAAAAGGGCC TTTCCGTCCT CAGCCGTCGC
     3601 TTCATGTGAC TCCACGGAGT ACCGGGCGCC GTCCAGGCAC CTCGATTAGT TCTCGAGCTT
     3661 TTGGAGTACG TCGTCTTTAG GTTGGGGGGA GGGGTTTTAT GCGATGGAGT TTCCCCACAC
     3721 TGAGTGGGTG GAGACTGAAG TTAGGCCAGC TTGGCACTTG ATGTAATTCT CCTTGGAATT
     3781 TGCCCTTTTT GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG TTCAAAGTTT
     3841 TTTTCTTCCA TTTCAGGTGT CGTGAcGTAC GgccaccATG AAAAGGCCGG CGGCCACGAA
     3901 AAAGGCCGGC CAGGCAAAAA AGAAAAAGGA CAAGAAGTAC AGCATCGGCC TGGCCATCGG
     3961 CACCAACTCT GTGGGCTGGG CCGTGATCAC CGACGAGTAC AAGGTGCCCA GCAAGAAATT
     4021 CAAGGTGCTG GGCAACACCG ACCGGCACAG CATCAAGAAG AACCTGATCG GAGCCCTGCT
     4081 GTTCGACAGC GGCGAAACAG CCGAGGCCAC CCGGCTGAAG AGAACCGCCA GAAGAAGATA
     4141 CACCAGACGG AAGAACCGGA TCTGCTATCT GCAAGAGATC TTCAGCAACG AGATGGCCAA
     4201 GGTGGACGAC AGCTTCTTCC ACAGACTGGA AGAGTCCTTC CTGGTGGAAG AGGATAAGAA
     4261 GCACGAGCGG CACCCCATCT TCGGCAACAT CGTGGACGAG GTGGCCTACC ACGAGAAGTA
     4321 CCCCACCATC TACCACCTGA GAAAGAAACT GGTGGACAGC ACCGACAAGG CCGACCTGCG
     4381 GCTGATCTAT CTGGCCCTGG CCCACATGAT CAAGTTCCGG GGCCACTTCC TGATCGAGGG
     4441 CGACCTGAAC CCCGACAACA GCGACGTGGA CAAGCTGTTC ATCCAGCTGG TGCAGACCTA
     4501 CAACCAGCTG TTCGAGGAAA ACCCCATCAA CGCCAGCGGC GTGGACGCCA AGGCCATCCT
     4561 GTCTGCCAGA CTGAGCAAGA GCAGACGGCT GGAAAATCTG ATCGCCCAGC TGCCCGGCGA
     4621 GAAGAAGAAT GGCCTGTTCG GCAACCTGAT TGCCCTGAGC CTGGGCCTGA CCCCCAACTT
     4681 CAAGAGCAAC TTCGACCTGG CCGAGGATGC CAAACTGCAG CTGAGCAAGG ACACCTACGA
     4741 CGACGACCTG GACAACCTGC TGGCCCAGAT CGGCGACCAG TACGCCGACC TGTTTCTGGC
     4801 CGCCAAGAAC CTGTCCGACG CCATCCTGCT GAGCGACATC CTGAGAGTGA ACACCGAGAT
     4861 CACCAAGGCC CCCCTGAGCG CCTCTATGAT CAAGAGATAC GACGAGCACC ACCAGGACCT
     4921 GACCCTGCTG AAAGCTCTCG TGCGGCAGCA GCTGCCTGAG AAGTACAAAG AGATTTTCTT
     4981 CGACCAGAGC AAGAACGGCT ACGCCGGCTA CATTGACGGC GGAGCCAGCC AGGAAGAGTT
     5041 CTACAAGTTC ATCAAGCCCA TCCTGGAAAA GATGGACGGC ACCGAGGAAC TGCTCGTGAA
     5101 GCTGAACAGA GAGGACCTGC TGCGGAAGCA GCGGACCTTC GACAACGGCA GCATCCCCCA
     5161 CCAGATCCAC CTGGGAGAGC TGCACGCCAT TCTGCGGCGG CAGGAAGATT TTTACCCATT
     5221 CCTGAAGGAC AACCGGGAAA AGATCGAGAA GATCCTGACC TTCCGCATCC CCTACTACGT
     5281 GGGCCCTCTG GCCAGGGGAA ACAGCAGATT CGCCTGGATG ACCAGAAAGA GCGAGGAAAC
     5341 CATCACCCCC TGGAACTTCG AGGAAGTGGT GGACAAGGGC GCTTCCGCCC AGAGCTTCAT
     5401 CGAGCGGATG ACCAACTTCG ATAAGAACCT GCCCAACGAG AAGGTGCTGC CCAAGCACAG
     5461 CCTGCTGTAC GAGTACTTCA CCGTGTATAA CGAGCTGACC AAAGTGAAAT ACGTGACCGA
     5521 GGGAATGAGA AAGCCCGCCT TCCTGAGCGG CGAGCAGAAA AAGGCCATCG TGGACCTGCT
     5581 GTTCAAGACC AACCGGAAAG TGACCGTGAA GCAGCTGAAA GAGGACTACT TCAAGAAAAT
     5641 CGAGTGCTTC GACTCCGTGG AAATCTCCGG CGTGGAAGAT CGGTTCAACG CCTCCCTGGG
     5701 CACATACCAC GATCTGCTGA AAATTATCAA GGACAAGGAC TTCCTGGACA ATGAGGAAAA
     5761 CGAGGACATT CTGGAAGATA TCGTGCTGAC CCTGACACTG TTTGAGGACA GAGAGATGAT
     5821 CGAGGAACGG CTGAAAACCT ATGCCCACCT GTTCGACGAC AAAGTGATGA AGCAGCTGAA
     5881 GCGGCGGAGA TACACCGGCT GGGGCAGGCT GAGCCGGAAG CTGATCAACG GCATCCGGGA
     5941 CAAGCAGTCC GGCAAGACAA TCCTGGATTT CCTGAAGTCC GACGGCTTCG CCAACAGAAA
     6001 CTTCATGCAG CTGATCCACG ACGACAGCCT GACCTTTAAA GAGGACATCC AGAAAGCCCA
     6061 GGTGTCCGGC CAGGGCGATA GCCTGCACGA GCACATTGCC AATCTGGCCG GCAGCCCCGC
     6121 CATTAAGAAG GGCATCCTGC AGACAGTGAA GGTGGTGGAC GAGCTCGTGA AAGTGATGGG
     6181 CCGGCACAAG CCCGAGAACA TCGTGATCGA AATGGCCAGA GAGAACCAGA CCACCCAGAA
     6241 GGGACAGAAG AACAGCCGCG AGAGAATGAA GCGGATCGAA GAGGGCATCA AAGAGCTGGG
     6301 CAGCCAGATC CTGAAAGAAC ACCCCGTGGA AAACACCCAG CTGCAGAACG AGAAGCTGTA
     6361 CCTGTACTAC CTGCAGAATG GGCGGGATAT GTACGTGGAC CAGGAACTGG ACATCAACCG
     6421 GCTGTCCGAC TACGATGTGG ACCACATCGT GCCTCAGAGC TTTCTGAAGG ACGACTCCAT
     6481 CGACAACAAG GTGCTGACCA GAAGCGACAA GGCCCGGGGC AAGAGCGACA ACGTGCCCTC
     6541 CGAAGAGGTC GTGAAGAAGA TGAAGAACTA CTGGCGGCAG CTGCTGAACG CCAAGCTGAT
     6601 TACCCAGAGA AAGTTCGACA ATCTGACCAA GGCCGAGAGA GGCGGCCTGA GCGAACTGGA
     6661 TAAGGCCGGC TTCATCAAGA GACAGCTGGT GGAAACCCGG CAGATCACAA AGCACGTGGC
     6721 ACAGATCCTG GACTCCCGGA TGAACACTAA GTACGACGAG AATGACAAGC TGATCCGGGA
     6781 AGTGAAAGTG ATCACCCTGA AGTCCAAGCT GGTGTCCGAT TTCCGGAAGG ATTTCCAGTT
     6841 TTACAAAGTG CGCGAGATCA ACAACTACCA CCACGCCCAC GACGCCTACC TGAACGCCGT
     6901 CGTGGGAACC GCCCTGATCA AAAAGTACCC TAAGCTGGAA AGCGAGTTCG TGTACGGCGA
     6961 CTACAAGGTG TACGACGTGC GGAAGATGAT CGCCAAGAGC GAGCAGGAAA TCGGCAAGGC
     7021 TACCGCCAAG TACTTCTTCT ACAGCAACAT CATGAACTTT TTCAAGACCG AGATTACCCT
     7081 GGCCAACGGC GAGATCCGGA AGCGGCCTCT GATCGAGACA AACGGCGAAA CCGGGGAGAT
     7141 CGTGTGGGAT AAGGGCCGGG ATTTTGCCAC CGTGCGGAAA GTGCTGAGCA TGCCCCAAGT
     7201 GAATATCGTG AAAAAGACCG AGGTGCAGAC AGGCGGCTTC AGCAAAGAGT CTATCCTGCC
     7261 CAAGAGGAAC AGCGATAAGC TGATCGCCAG AAAGAAGGAC TGGGACCCTA AGAAGTACGG
     7321 CGGCTTCGAC AGCCCCACCG TGGCCTATTC TGTGCTGGTG GTGGCCAAAG TGGAAAAGGG
     7381 CAAGTCCAAG AAACTGAAGA GTGTGAAAGA GCTGCTGGGG ATCACCATCA TGGAAAGAAG
     7441 CAGCTTCGAG AAGAATCCCA TCGACTTTCT GGAAGCCAAG GGCTACAAAG AAGTGAAAAA
     7501 GGACCTGATC ATCAAGCTGC CTAAGTACTC CCTGTTCGAG CTGGAAAACG GCCGGAAGAG
     7561 AATGCTGGCC TCTGCCGGCG AACTGCAGAA GGGAAACGAA CTGGCCCTGC CCTCCAAATA
     7621 TGTGAACTTC CTGTACCTGG CCAGCCACTA TGAGAAGCTG AAGGGCTCCC CCGAGGATAA
     7681 TGAGCAGAAA CAGCTGTTTG TGGAACAGCA CAAGCACTAC CTGGACGAGA TCATCGAGCA
     7741 GATCAGCGAG TTCTCCAAGA GAGTGATCCT GGCCGACGCT AATCTGGACA AAGTGCTGTC
     7801 CGCCTACAAC AAGCACCGGG ATAAGCCCAT CAGAGAGCAG GCCGAGAATA TCATCCACCT
     7861 GTTTACCCTG ACCAATCTGG GAGCCCCTGC CGCCTTCAAG TACTTTGACA CCACCATCGA
     7921 CCGGAAGAGG TACACCAGCA CCAAAGAGGT GCTGGACGCC ACCCTGATCC ACCAGAGCAT
     7981 CACCGGCCTG TACGAGACAC GGATCGACCT GTCTCAGCTG GGAGGCGACa gcgctGGAGG
     8041 AGGTGGAAGC GGAGGAGGAG GAAGCGGAGG AGGAGGTAGC ggacctaaga aaaagaggaa
     8101 ggtggcggcc gctggatccg actacaaaga ccatgacggt gattataaag atcatgacat
     8161 cgactacaag gatgacgatg acaagGGTgg ATCCggaTCA ggctccGATG CTAAGTCTCT
     8221 GACTGCCTGG TCCAGGACCC TTGTGACCTT CAAGGACGTC TTCGTTGATT TTACGCGGGA
     8281 AGAATGGAAA CTACTAGACA CAGCACAACA AATAGTATAC AGAAACGTAA TGCTAGAAAA
     8341 CTACAAAAAC CTAGTAAGCC TAGGATACCA ACTAACAAAA CCAGACGTAA TACTAAGACT
     8401 AGAAAAAGGA GAAGAACCAg gAtctgGAga gggAaggggA agcctcctaa cttgcggaga
     8461 Tgtcgaggag aatcctggcc caATGGTTAG TAAGGGAGAG GAGTTGATAA AAGAGAATAT
     8521 GCACATGAAA CTGTATATGG AAGGGACAGT GGACAACCAT CACTTCAAGT GCACCTCTGA
     8581 GGGAGAAGGA AAGCCATACG AAGGCACCCA GACTATGCGA ATTAAGGTCG TGGAGGGAGG
     8641 GCCTCTCCCC TTCGCCTTCG ATATTCTTGC AACATCTTTC CTCTACGGGA GCAAAACCTT
     8701 CATCAATCAC ACACAAGGCA TACCTGACTT TTTTAAGCAG AGTTTTCCAG AGGGCTTTAC
     8761 CTGGGAGCGT GTGACTACAT ACGAAGATGG GGGTGTGCTG ACGGCTACAC AGGATACTTC
     8821 ACTTCAGGAC GGTTGTCTGA TCTACAACGT CAAGATCAGG GGGGTCAATT TCACCTCGAA
     8881 TGGACCAGTA ATGCAAAAAA AGACCCTGGG ATGGGAAGCT TTTACTGAAA CTCTCTATCC
     8941 CGCCGACGGC GGCCTCGAAG GCAGAAACGA TATGGCTTTG AAGCTGGTTG GCGGGTCCCA
     9001 TCTAATTGCC AATGCCAAAA CCACATATAG GAGCAAAAAG CCTGCAAAGA ACCTGAAGAT
     9061 GCCGGGTGTG TACTATGTTG ACTATAGATT AGAGCGGATC AAAGAGGCAA ATAACGAAAC
     9121 GTACGTGGAG CAGCACGAGG TAGCCGTGGC GCGCTACTGC GATTTACCCT CCAAACTGGG
     9181 CCATAAACTG AACtgtacaG GAAGCGGAGC TACTAACTTC AGCCTGCTGA AGCAGGCTGG
     9241 cGACGTGGAa GAaAACCCaG GACCTatggc caagcctttg tctcaagaag aatccaccct
     9301 cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc
     9361 cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac
     9421 tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa
     9481 cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg
     9541 gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga
     9601 tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg
     9661 ctaagaattc gatatcaagc ttatcggtaa tcaacctctg gattacaaaa tttgtgaaag
     9721 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     9781 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     9841 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     9901 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     9961 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
    10021 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
    10081 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
    10141 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
    10201 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
    10261 ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat
    10321 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
    10381 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
    10441 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
    10501 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
    10561 ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta
    10621 caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg
    10681 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg
    10741 gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc cggactgtac
    10801 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    10861 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    10921 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    10981 cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
    11041 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
    11101 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
    11161 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
    11221 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
    11281 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
    11341 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
    11401 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
    11461 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
    11521 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
    11581 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
    11641 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
    11701 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
    11761 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
    11821 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
    11881 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
    11941 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
    12001 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
    12061 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
    12121 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
    12181 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
    12241 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
    12301 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
    12361 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
    12421 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
    12481 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
    12541 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
    12601 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
    12661 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    12721 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    12781 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
    12841 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
    12901 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
    12961 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    13021 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    13081 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    13141 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    13201 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    13261 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    13321 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    13381 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    13441 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    13501 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    13561 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    13621 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    13681 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    13741 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    13801 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    13861 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    13921 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    13981 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    14041 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    14101 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    14161 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    14221 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    14281 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    14341 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    14401 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    14461 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    14521 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    14581 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    14641 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    14701 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    14761 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    14821 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    14881 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    14941 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgac
//