LOCUS       pJT329_Lenti_pEF-rTetR( 9415 bp ds-DNA     circular     07-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    13..576
                     /label="pU6"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(516..525)
                     /label="GC Box"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    594..774
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    667..672
                     /label="Termination/PolyA motif"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(1351..1357)
                     /label="TATA box"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1445..1678
                     /label="RRE"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2209..2223
                     /label="cPPT"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2375..2405
                     /label="MCS"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2433..3394
                     /label="EF1Alpha"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3396..3605
                     /label="EF1Alpha"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             3615..3617
                     /label="Translation 3615-3617"
     misc_feature    3618..4235
                     /label="rTetR(SE-G72P)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             3618..4235
                     /label="Translation 3618-4235"
     misc_feature    4236..4301
                     /label="3XFLAG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             4236..4322
                     /label="Translation 4236-4322"
     misc_feature    4305..4322
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             4758..4763
                     /label="Translation 4758-4763"
     misc_feature    4758..4766
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             4764..4766
                     /label="Translation 4764-4766"
     misc_feature    4767..4820
                     /label="T2A"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             4767..4820
                     /label="Translation 4767-4820"
     CDS             4821..5528
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             4821..5528
                     /label="Translation 4821-5528"
     misc_feature    5529..5546
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             5529..5546
                     /label="Translation 5529-5546"
     CDS             5547..5945
                     /label="BSD"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             5547..5945
                     /label="Translation 5547-5945"
     misc_recomb     5965..5998
                     /label="LoxP recombination site"
                     /ApEinfo_revcolor=#4682b4
                     /ApEinfo_fwdcolor=#4682b4
     misc_feature    6044..6644
                     /label="wPRE"
                     /ApEinfo_revcolor=#0060ff
                     /ApEinfo_fwdcolor=#0060ff
     misc_feature    6834..6848
                     /label="cPPT"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    7171..7351
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(7395..8077)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#808080
                     /ApEinfo_fwdcolor=#808080
     misc_feature    complement(7398..8017)
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(7422..7431)
                     /label="GC Box"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(8175..8834)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(8175..8834)
                     /label="Translation 8834-8175"
     misc_feature    complement(9074..9102)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
       61 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      121 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      181 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      241 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      301 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      361 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      421 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      481 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      541 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      601 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      661 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      721 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      781 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
      841 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
      901 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
      961 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1021 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1081 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1141 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1201 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1261 aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct ggaggaggag
     1321 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1381 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1441 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1501 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     1561 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1621 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1681 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1741 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     1801 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     1861 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     1921 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     1981 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     2041 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     2101 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     2161 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2221 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2281 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2341 agcagagatc cagtttggtt agtaccgggc ccgctctaga GATCCCATTT GACTCAGgcg
     2401 gccgcgtgga taaccgtatt accgccatgc atGTGCCCGT CAGTGGGCAG AGCGCACATC
     2461 GCCCACAGTC CCCGAGAAGT TGGGGGGAGG GGTCGGCAAT TGAACCGGTG CCTAGAGAAG
     2521 GTGGCGCGGG GTAAACTGGG AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG
     2581 TGGGGGAGAA CCGTATATAA GTGCAGTAGT CGCCGTGAAC GTTCTTTTTC GCAACGGGTT
     2641 TGCCGCCAGA ACACAGGTAA GTGCCGTGTG TGGTTCCCGC GGGCCTGGCC TCTTTACGGG
     2701 TTATGGCCCT TGCGTGCCTT GAATTACTTC CACCTGGCTG CAGTACGTGA TTCTTGATCC
     2761 CGAGCTTCGG GTTGGAAGTG GGTGGGAGAG TTCGAGGCCT TGCGCTTAAG GAGCCCCTTC
     2821 GCCTCGTGCT TGAGTTGAGG CCTGGCCTGG GCGCTGGGGC CGCCGCGTGC GAATCTGGTG
     2881 GCACCTTCGC GCCTGTCTCG CTGCTTTCGA TAAGTCTCTA GCCATTTAAA ATTTTTGATG
     2941 ACCTGCTGCG ACGCTTTTTT TCTGGCAAGA TAGTCTTGTA AATGCGGGCC AAGATCTGCA
     3001 CACTGGTATT TCGGTTTTTG GGGCCGCGGG CGGCGACGGG GCCCGTGCGT CCCAGCGCAC
     3061 ATGTTCGGCG AGGCGGGGCC TGCGAGCGCG GCCACCGAGA ATCGGACGGG GGTAGTCTCA
     3121 AGCTGGCCGG CCTGCTCTGG TGCCTGGCCT CGCGCCGCCG TGTATCGCCC CGCCCTGGGC
     3181 GGCAAGGCTG GCCCGGTCGG CACCAGTTGC GTGAGCGGAA AGATGGCCGC TTCCCGGCCC
     3241 TGCTGCAGGG AGCTCAAAAT GGAGGACGCG GCGCTCGGGA GAGCGGGCGG GTGAGTCACC
     3301 CACACAAAGG AAAAGGGCCT TTCCGTCCTC AGCCGTCGCT TCATGTGACT CCACGGAGTA
     3361 CCGGGCGCCG TCCAGGCACC TCGATTAGTT CTCGcGCTTT TGGAGTACGT CGTCTTTAGG
     3421 TTGGGGGGAG GGGTTTTATG CGATGGAGTT TCCCCACACT GAGTGGGTGG AGACTGAAGT
     3481 TAGGCCAGCT TGGCACTTGA TGTAATTCTC CTTGGAATTT GCCCTTTTTG AGTTTGGATC
     3541 TTGGTTCATT CTCAAGCCTC AGACAGTGGT TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC
     3601 GTGAgCTAgc caccATGtct agactggaca agagcaaaAT Aataaacgga gctctggaat
     3661 tactcaatgg tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag
     3721 ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc
     3781 caatcgagat gctggacagg catcataccc acTCCtgccc cctggaaCCC gagtcatggc
     3841 aagactttct gcggaacaac gccaagtcat accgctgtgc tctcctctca catcgcgacg
     3901 gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc
     3961 agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg
     4021 ccgtgggcca ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag
     4081 aggaaagaga gacacctacc accgattcta tgcccccact tctgAAAcaa gcaattgagc
     4141 tgttcgaccg gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg
     4201 gcctggagaa acagctaaag tgcgaaagcg gcggtgacta caaagaccat gacggtgatt
     4261 ataaagatca tgacatcgac tacaaggatg acgatgacaa gGGTggATCC ggaTCAggct
     4321 ccGAGGGCCA GAGCGACGAG AGAGCCCTGC TGGACCAGCT GCACACCCTG CTGAGCAACA
     4381 CCGACGCCAC CGGCCTGGAG GAGATCGACA GAGCCCTGGG CATCCCCGAG CTGGTGAACC
     4441 AGGGCCAGGC CCTGGAGCCC AAGCAGGGAG GAGGAGGTAG CggaTTCGTG ACCCTGAAGG
     4501 ACGTGGGCAT GGACTTCACC CTGGGCGACT GGGAGCAGCT GGGCCTGGAG CAGGGCGACA
     4561 CCTTCTGGGA CACCGCCCTG GACAACTGCC AGGACCTGTT CCTGCTGGAC CCCCCCGGAG
     4621 GAGGAGGTAG CggaCACGAG AAGTTCCCCA GCGACCTGGA CCTGGACATG TTCAACGGCA
     4681 GCCTGGAGTG CGACATGGAG AGCATCATCA GAAGCGAGCT GATGGACGCC GACGGCCTGG
     4741 ACTTCAACTT CGACAGCggA tctgGAgagg gAaggggAag cctcctaact tgcggagaTg
     4801 tcgaggagaa tcctggccca atggtgagca agggcgagga ggataacatg gccatcatca
     4861 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
     4921 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     4981 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
     5041 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
     5101 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
     5161 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
     5221 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
     5281 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
     5341 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
     5401 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
     5461 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
     5521 tgtacaaggg tagcggcagc ggtagcatgg ccaagccttt gtctcaagaa gaatccaccc
     5581 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg
     5641 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
     5701 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
     5761 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
     5821 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg
     5881 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg
     5941 gctaagaatt cgtcgaggga cctaataact tcgtatagca tacattatac gaagttatac
     6001 atgtttaagg gttccggttc cactaggtac aattcgatat caagcttatc gataatcaac
     6061 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     6121 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     6181 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     6241 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     6301 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     6361 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     6421 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     6481 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     6541 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     6601 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     6661 gatcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     6721 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     6781 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     6841 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     6901 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc
     6961 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta
     7021 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg
     7081 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac
     7141 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc
     7201 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7261 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7321 cccttttagt cagtgtggaa aatctctagc agcatgtgag caaaaggcca gcaaaaggcc
     7381 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7441 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7501 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7561 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7621 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7681 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7741 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7801 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7861 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7921 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7981 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8041 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8101 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8161 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8221 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8281 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8341 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8401 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8461 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8521 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8581 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8641 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8701 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8761 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8821 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     8881 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8941 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9001 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9061 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9121 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgctta agcggtcgac
     9181 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
     9241 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     9301 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
     9361 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgaca
//