LOCUS       pJT337_Lenti_pEF-dCas9 15024 bp ds-DNA     circular     07-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     enhancer        152..531
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        533..731
                     /label="CMV promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     LTR             749..929
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    976..1101
                     /label="HIV-1 Ψ"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1594..1827
                     /label="RRE"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2354..2471
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        2601..3779
                     /label="EF-1α promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     intron          2832..3770
                     /label="EF-1α intron A"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     misc_feature    3786..3791
                     /label="kozak"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             3795..3842
                     /label="nucleoplasmin NLS"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             3843..7943
                     /label="dCas9"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             3843..9605
                     /label="Translation 3843-9605"
     CDS             7998..8018
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    8034..8099
                     /label="3XFLAG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             8034..8120
                     /label="Translation 8034-8120"
     misc_feature    8103..8120
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             8361..8366
                     /label="Translation 8361-8366"
     misc_feature    8361..8369
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             8367..8369
                     /label="Translation 8367-8369"
     misc_feature    8370..8423
                     /label="T2A"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             8370..8423
                     /label="Translation 8370-8423"
     misc_feature    8424..9134
                     /label="mTagBFP2 (benchling human codon opt)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             8424..9134
                     /label="Translation 8424-9134"
     misc_feature    9141..9206
                     /label="P2A"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             9141..9206
                     /label="Translation 9141-9206"
     CDS             9207..9605
                     /label="BSD"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    9630..10218
                     /label="WPRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(10101..10112)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     complement(10221..10237)
                     /label="KS primer"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     LTR             10743..10923
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     polyA_signal    10955..11179
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      11225..11653
                     /label="f1 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        11667..11996
                     /label="SV40 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      11847..11982
                     /label="SV40 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        12044..12091
                     /label="EM7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             12110..12484
                     /label="BleoR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     polyA_signal    12614..12735
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(12784..12800)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     protein_bind    12808..12824
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        complement(12832..12838)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(12839..12856)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(12857..12862)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    12877..12898
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     rep_origin      complement(13186..13774)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(13945..14736)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(14737..14805)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        complement(14806..14910)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
       61 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
      121 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
      181 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      241 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      301 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      361 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      421 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      481 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      541 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      601 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      661 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      721 tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
      781 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
      841 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
      901 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
      961 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     1021 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     1081 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     1141 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     1201 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     1261 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     1321 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     1381 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     1441 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     1501 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     1561 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     1621 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     1681 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     1741 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     1801 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     1861 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     1921 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     1981 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     2041 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     2101 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     2161 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     2221 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     2281 atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag
     2341 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2401 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2461 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta attagctagc
     2521 TGCAAAGATG GATAAAGTTT TAAACAGAGA GGAATCTTTG CAGCTAATGG ACCTTCTAGG
     2581 TCTTGAAAGG AGTGGGAATT GGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA
     2641 CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGAAC CGGTGCCTAG AGAAGGTGGC
     2701 GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG
     2761 GAGAACCGTA TATAAGTGCA GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG
     2821 CCAGAACACA GGTAAGTGCC GTGTGTGGTT CCCGCGGGCC TGGCCTCTTT ACGGGTTATG
     2881 GCCCTTGCGT GCCTTGAATT ACTTCCACCT GGCTGCAGTA CGTGATTCTT GATCCCGAGC
     2941 TTCGGGTTGG AAGTGGGTGG GAGAGTTCGA GGCCTTGCGC TTAAGGAGCC CCTTCGCCTC
     3001 GTGCTTGAGT TGAGGCCTGG CCTGGGCGCT GGGGCCGCCG CGTGCGAATC TGGTGGCACC
     3061 TTCGCGCCTG TCTCGCTGCT TTCGATAAGT CTCTAGCCAT TTAAAATTTT TGATGACCTG
     3121 CTGCGACGCT TTTTTTCTGG CAAGATAGTC TTGTAAATGC GGGCCAAGAT CTGCACACTG
     3181 GTATTTCGGT TTTTGGGGCC GCGGGCGGCG ACGGGGCCCG TGCGTCCCAG CGCACATGTT
     3241 CGGCGAGGCG GGGCCTGCGA GCGCGGCCAC CGAGAATCGG ACGGGGGTAG TCTCAAGCTG
     3301 GCCGGCCTGC TCTGGTGCCT GGCCTCGCGC CGCCGTGTAT CGCCCCGCCC TGGGCGGCAA
     3361 GGCTGGCCCG GTCGGCACCA GTTGCGTGAG CGGAAAGATG GCCGCTTCCC GGCCCTGCTG
     3421 CAGGGAGCTC AAAATGGAGG ACGCGGCGCT CGGGAGAGCG GGCGGGTGAG TCACCCACAC
     3481 AAAGGAAAAG GGCCTTTCCG TCCTCAGCCG TCGCTTCATG TGACTCCACG GAGTACCGGG
     3541 CGCCGTCCAG GCACCTCGAT TAGTTCTCGA GCTTTTGGAG TACGTCGTCT TTAGGTTGGG
     3601 GGGAGGGGTT TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC
     3661 CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT
     3721 TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAc
     3781 GTACGgccac cATGAAAAGG CCGGCGGCCA CGAAAAAGGC CGGCCAGGCA AAAAAGAAAA
     3841 AGGACAAGAA GTACAGCATC GGCCTGGCCA TCGGCACCAA CTCTGTGGGC TGGGCCGTGA
     3901 TCACCGACGA GTACAAGGTG CCCAGCAAGA AATTCAAGGT GCTGGGCAAC ACCGACCGGC
     3961 ACAGCATCAA GAAGAACCTG ATCGGAGCCC TGCTGTTCGA CAGCGGCGAA ACAGCCGAGG
     4021 CCACCCGGCT GAAGAGAACC GCCAGAAGAA GATACACCAG ACGGAAGAAC CGGATCTGCT
     4081 ATCTGCAAGA GATCTTCAGC AACGAGATGG CCAAGGTGGA CGACAGCTTC TTCCACAGAC
     4141 TGGAAGAGTC CTTCCTGGTG GAAGAGGATA AGAAGCACGA GCGGCACCCC ATCTTCGGCA
     4201 ACATCGTGGA CGAGGTGGCC TACCACGAGA AGTACCCCAC CATCTACCAC CTGAGAAAGA
     4261 AACTGGTGGA CAGCACCGAC AAGGCCGACC TGCGGCTGAT CTATCTGGCC CTGGCCCACA
     4321 TGATCAAGTT CCGGGGCCAC TTCCTGATCG AGGGCGACCT GAACCCCGAC AACAGCGACG
     4381 TGGACAAGCT GTTCATCCAG CTGGTGCAGA CCTACAACCA GCTGTTCGAG GAAAACCCCA
     4441 TCAACGCCAG CGGCGTGGAC GCCAAGGCCA TCCTGTCTGC CAGACTGAGC AAGAGCAGAC
     4501 GGCTGGAAAA TCTGATCGCC CAGCTGCCCG GCGAGAAGAA GAATGGCCTG TTCGGCAACC
     4561 TGATTGCCCT GAGCCTGGGC CTGACCCCCA ACTTCAAGAG CAACTTCGAC CTGGCCGAGG
     4621 ATGCCAAACT GCAGCTGAGC AAGGACACCT ACGACGACGA CCTGGACAAC CTGCTGGCCC
     4681 AGATCGGCGA CCAGTACGCC GACCTGTTTC TGGCCGCCAA GAACCTGTCC GACGCCATCC
     4741 TGCTGAGCGA CATCCTGAGA GTGAACACCG AGATCACCAA GGCCCCCCTG AGCGCCTCTA
     4801 TGATCAAGAG ATACGACGAG CACCACCAGG ACCTGACCCT GCTGAAAGCT CTCGTGCGGC
     4861 AGCAGCTGCC TGAGAAGTAC AAAGAGATTT TCTTCGACCA GAGCAAGAAC GGCTACGCCG
     4921 GCTACATTGA CGGCGGAGCC AGCCAGGAAG AGTTCTACAA GTTCATCAAG CCCATCCTGG
     4981 AAAAGATGGA CGGCACCGAG GAACTGCTCG TGAAGCTGAA CAGAGAGGAC CTGCTGCGGA
     5041 AGCAGCGGAC CTTCGACAAC GGCAGCATCC CCCACCAGAT CCACCTGGGA GAGCTGCACG
     5101 CCATTCTGCG GCGGCAGGAA GATTTTTACC CATTCCTGAA GGACAACCGG GAAAAGATCG
     5161 AGAAGATCCT GACCTTCCGC ATCCCCTACT ACGTGGGCCC TCTGGCCAGG GGAAACAGCA
     5221 GATTCGCCTG GATGACCAGA AAGAGCGAGG AAACCATCAC CCCCTGGAAC TTCGAGGAAG
     5281 TGGTGGACAA GGGCGCTTCC GCCCAGAGCT TCATCGAGCG GATGACCAAC TTCGATAAGA
     5341 ACCTGCCCAA CGAGAAGGTG CTGCCCAAGC ACAGCCTGCT GTACGAGTAC TTCACCGTGT
     5401 ATAACGAGCT GACCAAAGTG AAATACGTGA CCGAGGGAAT GAGAAAGCCC GCCTTCCTGA
     5461 GCGGCGAGCA GAAAAAGGCC ATCGTGGACC TGCTGTTCAA GACCAACCGG AAAGTGACCG
     5521 TGAAGCAGCT GAAAGAGGAC TACTTCAAGA AAATCGAGTG CTTCGACTCC GTGGAAATCT
     5581 CCGGCGTGGA AGATCGGTTC AACGCCTCCC TGGGCACATA CCACGATCTG CTGAAAATTA
     5641 TCAAGGACAA GGACTTCCTG GACAATGAGG AAAACGAGGA CATTCTGGAA GATATCGTGC
     5701 TGACCCTGAC ACTGTTTGAG GACAGAGAGA TGATCGAGGA ACGGCTGAAA ACCTATGCCC
     5761 ACCTGTTCGA CGACAAAGTG ATGAAGCAGC TGAAGCGGCG GAGATACACC GGCTGGGGCA
     5821 GGCTGAGCCG GAAGCTGATC AACGGCATCC GGGACAAGCA GTCCGGCAAG ACAATCCTGG
     5881 ATTTCCTGAA GTCCGACGGC TTCGCCAACA GAAACTTCAT GCAGCTGATC CACGACGACA
     5941 GCCTGACCTT TAAAGAGGAC ATCCAGAAAG CCCAGGTGTC CGGCCAGGGC GATAGCCTGC
     6001 ACGAGCACAT TGCCAATCTG GCCGGCAGCC CCGCCATTAA GAAGGGCATC CTGCAGACAG
     6061 TGAAGGTGGT GGACGAGCTC GTGAAAGTGA TGGGCCGGCA CAAGCCCGAG AACATCGTGA
     6121 TCGAAATGGC CAGAGAGAAC CAGACCACCC AGAAGGGACA GAAGAACAGC CGCGAGAGAA
     6181 TGAAGCGGAT CGAAGAGGGC ATCAAAGAGC TGGGCAGCCA GATCCTGAAA GAACACCCCG
     6241 TGGAAAACAC CCAGCTGCAG AACGAGAAGC TGTACCTGTA CTACCTGCAG AATGGGCGGG
     6301 ATATGTACGT GGACCAGGAA CTGGACATCA ACCGGCTGTC CGACTACGAT GTGGACCACA
     6361 TCGTGCCTCA GAGCTTTCTG AAGGACGACT CCATCGACAA CAAGGTGCTG ACCAGAAGCG
     6421 ACAAGGCCCG GGGCAAGAGC GACAACGTGC CCTCCGAAGA GGTCGTGAAG AAGATGAAGA
     6481 ACTACTGGCG GCAGCTGCTG AACGCCAAGC TGATTACCCA GAGAAAGTTC GACAATCTGA
     6541 CCAAGGCCGA GAGAGGCGGC CTGAGCGAAC TGGATAAGGC CGGCTTCATC AAGAGACAGC
     6601 TGGTGGAAAC CCGGCAGATC ACAAAGCACG TGGCACAGAT CCTGGACTCC CGGATGAACA
     6661 CTAAGTACGA CGAGAATGAC AAGCTGATCC GGGAAGTGAA AGTGATCACC CTGAAGTCCA
     6721 AGCTGGTGTC CGATTTCCGG AAGGATTTCC AGTTTTACAA AGTGCGCGAG ATCAACAACT
     6781 ACCACCACGC CCACGACGCC TACCTGAACG CCGTCGTGGG AACCGCCCTG ATCAAAAAGT
     6841 ACCCTAAGCT GGAAAGCGAG TTCGTGTACG GCGACTACAA GGTGTACGAC GTGCGGAAGA
     6901 TGATCGCCAA GAGCGAGCAG GAAATCGGCA AGGCTACCGC CAAGTACTTC TTCTACAGCA
     6961 ACATCATGAA CTTTTTCAAG ACCGAGATTA CCCTGGCCAA CGGCGAGATC CGGAAGCGGC
     7021 CTCTGATCGA GACAAACGGC GAAACCGGGG AGATCGTGTG GGATAAGGGC CGGGATTTTG
     7081 CCACCGTGCG GAAAGTGCTG AGCATGCCCC AAGTGAATAT CGTGAAAAAG ACCGAGGTGC
     7141 AGACAGGCGG CTTCAGCAAA GAGTCTATCC TGCCCAAGAG GAACAGCGAT AAGCTGATCG
     7201 CCAGAAAGAA GGACTGGGAC CCTAAGAAGT ACGGCGGCTT CGACAGCCCC ACCGTGGCCT
     7261 ATTCTGTGCT GGTGGTGGCC AAAGTGGAAA AGGGCAAGTC CAAGAAACTG AAGAGTGTGA
     7321 AAGAGCTGCT GGGGATCACC ATCATGGAAA GAAGCAGCTT CGAGAAGAAT CCCATCGACT
     7381 TTCTGGAAGC CAAGGGCTAC AAAGAAGTGA AAAAGGACCT GATCATCAAG CTGCCTAAGT
     7441 ACTCCCTGTT CGAGCTGGAA AACGGCCGGA AGAGAATGCT GGCCTCTGCC GGCGAACTGC
     7501 AGAAGGGAAA CGAACTGGCC CTGCCCTCCA AATATGTGAA CTTCCTGTAC CTGGCCAGCC
     7561 ACTATGAGAA GCTGAAGGGC TCCCCCGAGG ATAATGAGCA GAAACAGCTG TTTGTGGAAC
     7621 AGCACAAGCA CTACCTGGAC GAGATCATCG AGCAGATCAG CGAGTTCTCC AAGAGAGTGA
     7681 TCCTGGCCGA CGCTAATCTG GACAAAGTGC TGTCCGCCTA CAACAAGCAC CGGGATAAGC
     7741 CCATCAGAGA GCAGGCCGAG AATATCATCC ACCTGTTTAC CCTGACCAAT CTGGGAGCCC
     7801 CTGCCGCCTT CAAGTACTTT GACACCACCA TCGACCGGAA GAGGTACACC AGCACCAAAG
     7861 AGGTGCTGGA CGCCACCCTG ATCCACCAGA GCATCACCGG CCTGTACGAG ACACGGATCG
     7921 ACCTGTCTCA GCTGGGAGGC GACagcgctG GAGGAGGTGG AAGCGGAGGA GGAGGAAGCG
     7981 GAGGAGGAGG TAGCggacct aagaaaaaga ggaaggtggc ggccgctgga tccgactaca
     8041 aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagG
     8101 GTggATCCgg aTCAggctcc AACGTGGAGG TGGTGAAGGT GATGCCCCAG GACCTGGTGA
     8161 CCTTCAAGGA CGTGGCCATC GACTTCAGCC AGGAGGAGTG GCAGTGGATG AACCCCGCCC
     8221 AGAAGAGACT GTACAGAAGC ATGATGCTGG AGAACTACCA GAGCCTGGTG AGCCTGGGCC
     8281 TGTGCATCAG CAAGCCCTAC GTGATCAGCC TGCTGGAGCA GGGCAGAGAG CCCTGGGAGA
     8341 TGACCAGCGA GATGACCAGA ggAtctgGAg agggAagggg Aagcctccta acttgcggag
     8401 aTgtcgagga gaatcctggc ccaATGGTTA GTAAGGGAGA GGAGTTGATA AAAGAGAATA
     8461 TGCACATGAA ACTGTATATG GAAGGGACAG TGGACAACCA TCACTTCAAG TGCACCTCTG
     8521 AGGGAGAAGG AAAGCCATAC GAAGGCACCC AGACTATGCG AATTAAGGTC GTGGAGGGAG
     8581 GGCCTCTCCC CTTCGCCTTC GATATTCTTG CAACATCTTT CCTCTACGGG AGCAAAACCT
     8641 TCATCAATCA CACACAAGGC ATACCTGACT TTTTTAAGCA GAGTTTTCCA GAGGGCTTTA
     8701 CCTGGGAGCG TGTGACTACA TACGAAGATG GGGGTGTGCT GACGGCTACA CAGGATACTT
     8761 CACTTCAGGA CGGTTGTCTG ATCTACAACG TCAAGATCAG GGGGGTCAAT TTCACCTCGA
     8821 ATGGACCAGT AATGCAAAAA AAGACCCTGG GATGGGAAGC TTTTACTGAA ACTCTCTATC
     8881 CCGCCGACGG CGGCCTCGAA GGCAGAAACG ATATGGCTTT GAAGCTGGTT GGCGGGTCCC
     8941 ATCTAATTGC CAATGCCAAA ACCACATATA GGAGCAAAAA GCCTGCAAAG AACCTGAAGA
     9001 TGCCGGGTGT GTACTATGTT GACTATAGAT TAGAGCGGAT CAAAGAGGCA AATAACGAAA
     9061 CGTACGTGGA GCAGCACGAG GTAGCCGTGG CGCGCTACTG CGATTTACCC TCCAAACTGG
     9121 GCCATAAACT GAACtgtaca GGAAGCGGAG CTACTAACTT CAGCCTGCTG AAGCAGGCTG
     9181 GcGACGTGGA aGAaAACCCa GGACCTatgg ccaagccttt gtctcaagaa gaatccaccc
     9241 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg
     9301 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
     9361 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
     9421 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
     9481 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg
     9541 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg
     9601 gctaagaatt cgatatcaag cttatcggta atcaacctct ggattacaaa atttgtgaaa
     9661 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     9721 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     9781 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     9841 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     9901 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     9961 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
    10021 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
    10081 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
    10141 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
    10201 tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa
    10261 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
    10321 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
    10381 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
    10441 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
    10501 attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct
    10561 acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc
    10621 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt
    10681 ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta
    10741 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
    10801 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
    10861 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
    10921 gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
    10981 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
    11041 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
    11101 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
    11161 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
    11221 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
    11281 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
    11341 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
    11401 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
    11461 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
    11521 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
    11581 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
    11641 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
    11701 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
    11761 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
    11821 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
    11881 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
    11941 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
    12001 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
    12061 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt
    12121 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
    12181 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
    12241 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
    12301 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
    12361 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
    12421 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
    12481 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
    12541 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
    12601 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    12661 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    12721 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
    12781 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
    12841 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
    12901 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    12961 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    13021 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    13081 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    13141 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    13201 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    13261 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    13321 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    13381 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    13441 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    13501 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    13561 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    13621 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    13681 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    13741 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    13801 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    13861 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    13921 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    13981 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    14041 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    14101 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    14161 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    14221 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    14281 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    14341 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    14401 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    14461 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    14521 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    14581 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    14641 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    14701 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    14761 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    14821 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    14881 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    14941 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
    15001 gccgcatagt taagccagta tctg
//