LOCUS       pJT351_Lenti_pEF-dCas9 15024 bp ds-DNA     circular     07-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     enhancer        257..636
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        638..836
                     /label="CMV promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     LTR             854..1034
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1081..1206
                     /label="HIV-1 Ψ"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1699..1932
                     /label="RRE"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2459..2576
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        2706..3884
                     /label="EF-1α promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     intron          2937..3875
                     /label="EF-1α intron A"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     misc_feature    3891..3896
                     /label="kozak"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             3900..3947
                     /label="nucleoplasmin NLS"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             3948..8048
                     /label="dCas9"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             3948..9710
                     /label="Translation 3948-9710"
     CDS             8103..8123
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    8139..8204
                     /label="3XFLAG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             8139..8225
                     /label="Translation 8139-8225"
     misc_feature    8208..8225
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             8466..8471
                     /label="Translation 8466-8471"
     misc_feature    8466..8474
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             8472..8474
                     /label="Translation 8472-8474"
     misc_feature    8475..8528
                     /label="T2A"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             8475..8528
                     /label="Translation 8475-8528"
     misc_feature    8529..9239
                     /label="mTagBFP2 (benchling human codon opt)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             8529..9239
                     /label="Translation 8529-9239"
     misc_feature    9246..9311
                     /label="P2A"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             9246..9311
                     /label="Translation 9246-9311"
     CDS             9312..9710
                     /label="BSD"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    9735..10323
                     /label="WPRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(10206..10217)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     complement(10326..10342)
                     /label="KS primer"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     LTR             10848..11028
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     polyA_signal    11060..11284
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      11330..11758
                     /label="f1 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        11772..12101
                     /label="SV40 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      11952..12087
                     /label="SV40 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        12149..12196
                     /label="EM7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             12215..12589
                     /label="BleoR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     polyA_signal    12719..12840
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(12889..12905)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     protein_bind    12913..12929
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        complement(12937..12943)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(12944..12961)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(12962..12967)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    12982..13003
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     rep_origin      complement(13291..13879)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(14050..14841)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(14842..14910)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        complement(14911..15015)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
       61 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
      121 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
      181 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
      241 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
      301 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      361 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      421 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      481 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      541 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      601 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      661 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      721 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      781 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
      841 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
      901 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
      961 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     1021 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     1081 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     1141 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     1201 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     1261 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     1321 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     1381 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     1441 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     1501 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     1561 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     1621 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     1681 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     1741 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     1801 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     1861 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     1921 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     1981 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     2041 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     2101 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     2161 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     2221 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     2281 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     2341 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     2401 gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt
     2461 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     2521 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     2581 tttattacag ggacagcaga gatccagttt ggttaattag ctagcTGCAA AGATGGATAA
     2641 AGTTTTAAAC AGAGAGGAAT CTTTGCAGCT AATGGACCTT CTAGGTCTTG AAAGGAGTGG
     2701 GAATTGGCTC CGGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT
     2761 TGGGGGGAGG GGTCGGCAAT TGAACCGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG
     2821 AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA
     2881 GTGCAGTAGT CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGGTAA
     2941 GTGCCGTGTG TGGTTCCCGC GGGCCTGGCC TCTTTACGGG TTATGGCCCT TGCGTGCCTT
     3001 GAATTACTTC CACCTGGCTG CAGTACGTGA TTCTTGATCC CGAGCTTCGG GTTGGAAGTG
     3061 GGTGGGAGAG TTCGAGGCCT TGCGCTTAAG GAGCCCCTTC GCCTCGTGCT TGAGTTGAGG
     3121 CCTGGCCTGG GCGCTGGGGC CGCCGCGTGC GAATCTGGTG GCACCTTCGC GCCTGTCTCG
     3181 CTGCTTTCGA TAAGTCTCTA GCCATTTAAA ATTTTTGATG ACCTGCTGCG ACGCTTTTTT
     3241 TCTGGCAAGA TAGTCTTGTA AATGCGGGCC AAGATCTGCA CACTGGTATT TCGGTTTTTG
     3301 GGGCCGCGGG CGGCGACGGG GCCCGTGCGT CCCAGCGCAC ATGTTCGGCG AGGCGGGGCC
     3361 TGCGAGCGCG GCCACCGAGA ATCGGACGGG GGTAGTCTCA AGCTGGCCGG CCTGCTCTGG
     3421 TGCCTGGCCT CGCGCCGCCG TGTATCGCCC CGCCCTGGGC GGCAAGGCTG GCCCGGTCGG
     3481 CACCAGTTGC GTGAGCGGAA AGATGGCCGC TTCCCGGCCC TGCTGCAGGG AGCTCAAAAT
     3541 GGAGGACGCG GCGCTCGGGA GAGCGGGCGG GTGAGTCACC CACACAAAGG AAAAGGGCCT
     3601 TTCCGTCCTC AGCCGTCGCT TCATGTGACT CCACGGAGTA CCGGGCGCCG TCCAGGCACC
     3661 TCGATTAGTT CTCGAGCTTT TGGAGTACGT CGTCTTTAGG TTGGGGGGAG GGGTTTTATG
     3721 CGATGGAGTT TCCCCACACT GAGTGGGTGG AGACTGAAGT TAGGCCAGCT TGGCACTTGA
     3781 TGTAATTCTC CTTGGAATTT GCCCTTTTTG AGTTTGGATC TTGGTTCATT CTCAAGCCTC
     3841 AGACAGTGGT TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC GTGAcGTACG gccaccATGA
     3901 AAAGGCCGGC GGCCACGAAA AAGGCCGGCC AGGCAAAAAA GAAAAAGGAC AAGAAGTACA
     3961 GCATCGGCCT GGCCATCGGC ACCAACTCTG TGGGCTGGGC CGTGATCACC GACGAGTACA
     4021 AGGTGCCCAG CAAGAAATTC AAGGTGCTGG GCAACACCGA CCGGCACAGC ATCAAGAAGA
     4081 ACCTGATCGG AGCCCTGCTG TTCGACAGCG GCGAAACAGC CGAGGCCACC CGGCTGAAGA
     4141 GAACCGCCAG AAGAAGATAC ACCAGACGGA AGAACCGGAT CTGCTATCTG CAAGAGATCT
     4201 TCAGCAACGA GATGGCCAAG GTGGACGACA GCTTCTTCCA CAGACTGGAA GAGTCCTTCC
     4261 TGGTGGAAGA GGATAAGAAG CACGAGCGGC ACCCCATCTT CGGCAACATC GTGGACGAGG
     4321 TGGCCTACCA CGAGAAGTAC CCCACCATCT ACCACCTGAG AAAGAAACTG GTGGACAGCA
     4381 CCGACAAGGC CGACCTGCGG CTGATCTATC TGGCCCTGGC CCACATGATC AAGTTCCGGG
     4441 GCCACTTCCT GATCGAGGGC GACCTGAACC CCGACAACAG CGACGTGGAC AAGCTGTTCA
     4501 TCCAGCTGGT GCAGACCTAC AACCAGCTGT TCGAGGAAAA CCCCATCAAC GCCAGCGGCG
     4561 TGGACGCCAA GGCCATCCTG TCTGCCAGAC TGAGCAAGAG CAGACGGCTG GAAAATCTGA
     4621 TCGCCCAGCT GCCCGGCGAG AAGAAGAATG GCCTGTTCGG CAACCTGATT GCCCTGAGCC
     4681 TGGGCCTGAC CCCCAACTTC AAGAGCAACT TCGACCTGGC CGAGGATGCC AAACTGCAGC
     4741 TGAGCAAGGA CACCTACGAC GACGACCTGG ACAACCTGCT GGCCCAGATC GGCGACCAGT
     4801 ACGCCGACCT GTTTCTGGCC GCCAAGAACC TGTCCGACGC CATCCTGCTG AGCGACATCC
     4861 TGAGAGTGAA CACCGAGATC ACCAAGGCCC CCCTGAGCGC CTCTATGATC AAGAGATACG
     4921 ACGAGCACCA CCAGGACCTG ACCCTGCTGA AAGCTCTCGT GCGGCAGCAG CTGCCTGAGA
     4981 AGTACAAAGA GATTTTCTTC GACCAGAGCA AGAACGGCTA CGCCGGCTAC ATTGACGGCG
     5041 GAGCCAGCCA GGAAGAGTTC TACAAGTTCA TCAAGCCCAT CCTGGAAAAG ATGGACGGCA
     5101 CCGAGGAACT GCTCGTGAAG CTGAACAGAG AGGACCTGCT GCGGAAGCAG CGGACCTTCG
     5161 ACAACGGCAG CATCCCCCAC CAGATCCACC TGGGAGAGCT GCACGCCATT CTGCGGCGGC
     5221 AGGAAGATTT TTACCCATTC CTGAAGGACA ACCGGGAAAA GATCGAGAAG ATCCTGACCT
     5281 TCCGCATCCC CTACTACGTG GGCCCTCTGG CCAGGGGAAA CAGCAGATTC GCCTGGATGA
     5341 CCAGAAAGAG CGAGGAAACC ATCACCCCCT GGAACTTCGA GGAAGTGGTG GACAAGGGCG
     5401 CTTCCGCCCA GAGCTTCATC GAGCGGATGA CCAACTTCGA TAAGAACCTG CCCAACGAGA
     5461 AGGTGCTGCC CAAGCACAGC CTGCTGTACG AGTACTTCAC CGTGTATAAC GAGCTGACCA
     5521 AAGTGAAATA CGTGACCGAG GGAATGAGAA AGCCCGCCTT CCTGAGCGGC GAGCAGAAAA
     5581 AGGCCATCGT GGACCTGCTG TTCAAGACCA ACCGGAAAGT GACCGTGAAG CAGCTGAAAG
     5641 AGGACTACTT CAAGAAAATC GAGTGCTTCG ACTCCGTGGA AATCTCCGGC GTGGAAGATC
     5701 GGTTCAACGC CTCCCTGGGC ACATACCACG ATCTGCTGAA AATTATCAAG GACAAGGACT
     5761 TCCTGGACAA TGAGGAAAAC GAGGACATTC TGGAAGATAT CGTGCTGACC CTGACACTGT
     5821 TTGAGGACAG AGAGATGATC GAGGAACGGC TGAAAACCTA TGCCCACCTG TTCGACGACA
     5881 AAGTGATGAA GCAGCTGAAG CGGCGGAGAT ACACCGGCTG GGGCAGGCTG AGCCGGAAGC
     5941 TGATCAACGG CATCCGGGAC AAGCAGTCCG GCAAGACAAT CCTGGATTTC CTGAAGTCCG
     6001 ACGGCTTCGC CAACAGAAAC TTCATGCAGC TGATCCACGA CGACAGCCTG ACCTTTAAAG
     6061 AGGACATCCA GAAAGCCCAG GTGTCCGGCC AGGGCGATAG CCTGCACGAG CACATTGCCA
     6121 ATCTGGCCGG CAGCCCCGCC ATTAAGAAGG GCATCCTGCA GACAGTGAAG GTGGTGGACG
     6181 AGCTCGTGAA AGTGATGGGC CGGCACAAGC CCGAGAACAT CGTGATCGAA ATGGCCAGAG
     6241 AGAACCAGAC CACCCAGAAG GGACAGAAGA ACAGCCGCGA GAGAATGAAG CGGATCGAAG
     6301 AGGGCATCAA AGAGCTGGGC AGCCAGATCC TGAAAGAACA CCCCGTGGAA AACACCCAGC
     6361 TGCAGAACGA GAAGCTGTAC CTGTACTACC TGCAGAATGG GCGGGATATG TACGTGGACC
     6421 AGGAACTGGA CATCAACCGG CTGTCCGACT ACGATGTGGA CCACATCGTG CCTCAGAGCT
     6481 TTCTGAAGGA CGACTCCATC GACAACAAGG TGCTGACCAG AAGCGACAAG GCCCGGGGCA
     6541 AGAGCGACAA CGTGCCCTCC GAAGAGGTCG TGAAGAAGAT GAAGAACTAC TGGCGGCAGC
     6601 TGCTGAACGC CAAGCTGATT ACCCAGAGAA AGTTCGACAA TCTGACCAAG GCCGAGAGAG
     6661 GCGGCCTGAG CGAACTGGAT AAGGCCGGCT TCATCAAGAG ACAGCTGGTG GAAACCCGGC
     6721 AGATCACAAA GCACGTGGCA CAGATCCTGG ACTCCCGGAT GAACACTAAG TACGACGAGA
     6781 ATGACAAGCT GATCCGGGAA GTGAAAGTGA TCACCCTGAA GTCCAAGCTG GTGTCCGATT
     6841 TCCGGAAGGA TTTCCAGTTT TACAAAGTGC GCGAGATCAA CAACTACCAC CACGCCCACG
     6901 ACGCCTACCT GAACGCCGTC GTGGGAACCG CCCTGATCAA AAAGTACCCT AAGCTGGAAA
     6961 GCGAGTTCGT GTACGGCGAC TACAAGGTGT ACGACGTGCG GAAGATGATC GCCAAGAGCG
     7021 AGCAGGAAAT CGGCAAGGCT ACCGCCAAGT ACTTCTTCTA CAGCAACATC ATGAACTTTT
     7081 TCAAGACCGA GATTACCCTG GCCAACGGCG AGATCCGGAA GCGGCCTCTG ATCGAGACAA
     7141 ACGGCGAAAC CGGGGAGATC GTGTGGGATA AGGGCCGGGA TTTTGCCACC GTGCGGAAAG
     7201 TGCTGAGCAT GCCCCAAGTG AATATCGTGA AAAAGACCGA GGTGCAGACA GGCGGCTTCA
     7261 GCAAAGAGTC TATCCTGCCC AAGAGGAACA GCGATAAGCT GATCGCCAGA AAGAAGGACT
     7321 GGGACCCTAA GAAGTACGGC GGCTTCGACA GCCCCACCGT GGCCTATTCT GTGCTGGTGG
     7381 TGGCCAAAGT GGAAAAGGGC AAGTCCAAGA AACTGAAGAG TGTGAAAGAG CTGCTGGGGA
     7441 TCACCATCAT GGAAAGAAGC AGCTTCGAGA AGAATCCCAT CGACTTTCTG GAAGCCAAGG
     7501 GCTACAAAGA AGTGAAAAAG GACCTGATCA TCAAGCTGCC TAAGTACTCC CTGTTCGAGC
     7561 TGGAAAACGG CCGGAAGAGA ATGCTGGCCT CTGCCGGCGA ACTGCAGAAG GGAAACGAAC
     7621 TGGCCCTGCC CTCCAAATAT GTGAACTTCC TGTACCTGGC CAGCCACTAT GAGAAGCTGA
     7681 AGGGCTCCCC CGAGGATAAT GAGCAGAAAC AGCTGTTTGT GGAACAGCAC AAGCACTACC
     7741 TGGACGAGAT CATCGAGCAG ATCAGCGAGT TCTCCAAGAG AGTGATCCTG GCCGACGCTA
     7801 ATCTGGACAA AGTGCTGTCC GCCTACAACA AGCACCGGGA TAAGCCCATC AGAGAGCAGG
     7861 CCGAGAATAT CATCCACCTG TTTACCCTGA CCAATCTGGG AGCCCCTGCC GCCTTCAAGT
     7921 ACTTTGACAC CACCATCGAC CGGAAGAGGT ACACCAGCAC CAAAGAGGTG CTGGACGCCA
     7981 CCCTGATCCA CCAGAGCATC ACCGGCCTGT ACGAGACACG GATCGACCTG TCTCAGCTGG
     8041 GAGGCGACag cgctGGAGGA GGTGGAAGCG GAGGAGGAGG AAGCGGAGGA GGAGGTAGCg
     8101 gacctaagaa aaagaggaag gtggcggccg ctggatccga ctacaaagac catgacggtg
     8161 attataaaga tcatgacatc gactacaagg atgacgatga caagGGTggA TCCggaTCAg
     8221 gctccAACGG CCTGACCACC GTGGCCCTGA AGGAGACCAG CACCGAGGCC CTGATGAAGA
     8281 GCAGCCCCGA GGAGAGAGAG AGAATGATCA AGCAGCTGAA GGAGGAGCTG AGACTGGAGG
     8341 AGGCCAAGCT GGTGCTGCTG AAGAAGCTGA GACAGAGCCA GATCCAGAAG GAGGCCACCG
     8401 CCCAGAAGCC CACCGGCAGC GTGGGCAGCA CCGTGACCAC ACCCCCTCCC CTGGTGAGAG
     8461 GCACCggAtc tgGAgagggA aggggAagcc tcctaacttg cggagaTgtc gaggagaatc
     8521 ctggcccaAT GGTTAGTAAG GGAGAGGAGT TGATAAAAGA GAATATGCAC ATGAAACTGT
     8581 ATATGGAAGG GACAGTGGAC AACCATCACT TCAAGTGCAC CTCTGAGGGA GAAGGAAAGC
     8641 CATACGAAGG CACCCAGACT ATGCGAATTA AGGTCGTGGA GGGAGGGCCT CTCCCCTTCG
     8701 CCTTCGATAT TCTTGCAACA TCTTTCCTCT ACGGGAGCAA AACCTTCATC AATCACACAC
     8761 AAGGCATACC TGACTTTTTT AAGCAGAGTT TTCCAGAGGG CTTTACCTGG GAGCGTGTGA
     8821 CTACATACGA AGATGGGGGT GTGCTGACGG CTACACAGGA TACTTCACTT CAGGACGGTT
     8881 GTCTGATCTA CAACGTCAAG ATCAGGGGGG TCAATTTCAC CTCGAATGGA CCAGTAATGC
     8941 AAAAAAAGAC CCTGGGATGG GAAGCTTTTA CTGAAACTCT CTATCCCGCC GACGGCGGCC
     9001 TCGAAGGCAG AAACGATATG GCTTTGAAGC TGGTTGGCGG GTCCCATCTA ATTGCCAATG
     9061 CCAAAACCAC ATATAGGAGC AAAAAGCCTG CAAAGAACCT GAAGATGCCG GGTGTGTACT
     9121 ATGTTGACTA TAGATTAGAG CGGATCAAAG AGGCAAATAA CGAAACGTAC GTGGAGCAGC
     9181 ACGAGGTAGC CGTGGCGCGC TACTGCGATT TACCCTCCAA ACTGGGCCAT AAACTGAACt
     9241 gtacaGGAAG CGGAGCTACT AACTTCAGCC TGCTGAAGCA GGCTGGcGAC GTGGAaGAaA
     9301 ACCCaGGACC Tatggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa
     9361 cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct
     9421 ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg
     9481 cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg
     9541 tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc
     9601 ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg
     9661 cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gaattcgata
     9721 tcaagcttat cggtaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     9781 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     9841 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     9901 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     9961 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
    10021 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
    10081 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
    10141 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
    10201 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
    10261 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
    10321 cgcatcgata ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata
    10381 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
    10441 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
    10501 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
    10561 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca
    10621 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag
    10681 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
    10741 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
    10801 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt
    10861 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
    10921 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
    10981 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa
    11041 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
    11101 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
    11161 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
    11221 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
    11281 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
    11341 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
    11401 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
    11461 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
    11521 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
    11581 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
    11641 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
    11701 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
    11761 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
    11821 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
    11881 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
    11941 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
    12001 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
    12061 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
    12121 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
    12181 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
    12241 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
    12301 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
    12361 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
    12421 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
    12481 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
    12541 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg
    12601 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
    12661 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
    12721 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    12781 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    12841 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
    12901 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    12961 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    13021 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    13081 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    13141 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    13201 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    13261 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    13321 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    13381 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    13441 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    13501 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    13561 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    13621 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    13681 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    13741 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    13801 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    13861 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    13921 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    13981 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    14041 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    14101 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    14161 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    14221 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    14281 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    14341 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    14401 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    14461 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    14521 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    14581 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    14641 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    14701 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    14761 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    14821 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    14881 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    14941 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    15001 aataggggtt ccgcgcacat ttcc
//