LOCUS       Exported                8076 bp DNA     circular SYN 07-JUN-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 7, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
COMMENT     [DEFINITION]: 
[LOCUS]: pEGFPC1
FEATURES             Location/Qualifiers
     source          1..8076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    606..617
                     /label=Kozak
                     /note="color: #8000ff"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     misc_feature    613..1329
                     /label=EGFP
                     /note="color: #00ff40; direction: RIGHT"
     CDS             613..1329
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1:  613 ..  615 / #05fd14
                        2:  616 ..  618 / #05fd14 / 1a
                        3:  619 .. 1329 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    1330..1364
                     /label=MCS
                     /note="color: #0080ff"
     misc_feature    1365..1383
                     /label=sequence coming from the cloning process
                     /note="color: #008080; direction: RIGHT"
     misc_feature    1384..4707
                     /label=Myo1b ORF
                     /note="color: #800000; direction: RIGHT"
     polyA_signal    4864..4985
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(4992..5447)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        5474..5578
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        5580..5937
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      5788..5923
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             5972..6766
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6998..7045
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
                     /note="color: #a6acb3"
     rep_origin      7374..7962
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attcggatcc tagatatcca
     1381 cccatggcca agaaggaggt aaaatcctca cttctggaca atatgatcgg cgttggagat
     1441 acggttctct tggagcctct caacgaggag acgttcatcg acaacctgaa gaagcgcttc
     1501 gatcacaatg agatatatac atacatcggg agtgtggtta tatctgttaa cccctaccgg
     1561 tccttgccca tttattcacc agagaaagtg gaagattaca gaaatagaaa cttctatgaa
     1621 ctgagccctc acatctttgc tttgtcggat gaagcataca gatccctccg tgatcaagat
     1681 aaagatcagt gcattctcat tactggggag agtggagcgg gaaaaacaga ggccagcaag
     1741 cttgtgatgt cctatgtcgc tgcagtgtgt ggaaaaggag cagaagttaa tcaggtcaag
     1801 gaacagcttc tccagtccac ccctgtcctg gaagcttttg gaaatgccaa gactgtaagg
     1861 aatgacaatt catccagatt cggcaaatac atggatattg aatttgactt taaaggcgat
     1921 ccgctgggag gagtgataag taactatctt ttagagaaat ctcgtgttgt taagcagccg
     1981 agaggtgaaa gaaacttcca cgtgttctat cagctgctct cgggtgcctc tgaggaactc
     2041 ctccacaagc ttaaactgga gcgggatttc agcagataca actatctgag tctggactct
     2101 gccaaagtga atggtgtgga cgacgctgcc aatttcagaa ctgtcaggaa tgccatgcag
     2161 atcgtgggct tttcggatcc tgaggccgag tctgtcctgg aggttgtggc agctgtcttg
     2221 aaactgggga acatcgagtt caagcctgaa tcacgaatga acggtttgga tgaaagcaaa
     2281 atcaaagaca aaaatgagtt aaaagaaatt tgtgagttga ccagcatcga tcaagtggtt
     2341 ttagaaagag cgttcagttt ccgaacggtt gaggcgaagc aggagaaagt ttcaaccaca
     2401 ttgaatgtgg ctcaggctta ttatgcccgt gatgccctgg ctaaaaacct ctacagccgg
     2461 ttgttttcat ggttggtaaa tcgaatcaac gaaagcatta aggcacaaac caaagtgaga
     2521 aagaaggtca tgggtgttct ggacatttat ggctttgaga tatttgagga caacagcttc
     2581 gagcagttca ttattaatta ttgtaatgaa aagcttcaac aaatcttcat tgaactgacc
     2641 ctcaaagaag agcaagagga atatattcgt gaggacatag aatggactca cattgactac
     2701 ttcaacaatg ctatcatttg cgacctaata gaaaacaaca caaacggaat cttggccatg
     2761 ctggatgaag agtgcctgag acccggcact gttacagatg agaccttcct ggaaaagctg
     2821 aaccaagtct gtgccaccca ccagcacttc gagagcagga tgagcaaatg ttcccgcttc
     2881 ctcaatgaca cgaccctgcc tcacagctgc ttcagaatcc agcattatgc tggcaaggtg
     2941 ctataccagg tggaagggtt tgttgacaag aacaatgacc ttctgtaccg agacctgtct
     3001 caagccatgt ggaaggctgg ccatgccctc atcaagtctc tgttccctga agggaatcct
     3061 gcaaaggtca acctgaaaag acctcctact gcagggtccc agttcaaggc gtcggtggcc
     3121 accctgatga aaaacctgca gaccaagaac ccgaactata tcaggtgcat caagccgaac
     3181 gataaaaagg ccgctcacat tttcagtgag agtctcgtgt gccatcagat ccggtacctg
     3241 ggtcttctgg agaacgttcg agtgagacgg gcaggctatg ccttcaggca ggcctatgaa
     3301 ccttgtctgg aaagatacaa gatgctttgc aaacaaacat ggcctcactg gaagggacca
     3361 gcaaggtctg gtgtggaagt tctgtttaat gagctagaga ttcccgtgga agagtactcc
     3421 ttcggtagat ccaagatatt catccgaaac ccaaggacat tattccaatt agaagaccta
     3481 aggaagcagc ggctggagga cctggccact ctcattcaga agatttatcg aggatggaaa
     3541 tgtcgtacac attttctgct catgaaaaga agccaagtcg tgattgccgc ctggtacagg
     3601 agatacgcgc aacaaaagcg atatcagcag ataaagagtt cagccctggt gattcagtct
     3661 tacatccggg gctggaaggc tagaaaaatc ctgcgggagc tgaagcatca gaaacgctgc
     3721 aaggaggcag ccaccaccat cgcagcatac tggcacggca cccaggcgcg aagggaattg
     3781 aaacgcttga aggaggaggc taggcgtaag catgcagttg ctgtcatttg ggcttactgg
     3841 cttggactga aggtacgaag ggagtacagg aaattcttta gagcaaatgc tggaaagaaa
     3901 atctatgagt ttacacttca aagaattgtg caaaaatact tgttggaaat gaaaaataaa
     3961 atgccttcct tgtcaccaat agataaaaat tggccatcaa gaccttacct attcttggat
     4021 tcgactcaca aggagctaaa aaggatcttc cacttgtgga ggtgtaaaaa atacagggat
     4081 caattcacag accagcagaa acttatttat gaagagaagc tggaggccag tgaactcttc
     4141 aaagacaaga aggctttata tccttctagt gttggtcagc cattccaagg agcttacctg
     4201 gaaatcaaca agaacccaaa atataagaaa ctcaaagatg ccattgaaga gaagatcatt
     4261 attgctgagg ttgtgaacaa aatcaaccgg gctaatggga agagtacatc tcggattttc
     4321 ctcttaacaa acaataacct tctccttgct gaccaaaagt cggggcagat caagtccgag
     4381 gtccccctgg tggatgtgac taaggtctcc atgagttcac agaatgatgg tttctttgca
     4441 gtacacctca aggagggttc agaagcagct agtaagggtg actttctctt cagcagtgac
     4501 cacctgattg aaatggcaac caagttgtat cgcacgactc ttagccaaac taaacagaag
     4561 ctcaatatcg agatttccga tgagttcctg gtacagttca gacaggacaa agtatgtgtg
     4621 aagtttattc aaggcaacca gaaaaacggg agcgtgccaa cctgcaaacg aaagaacaac
     4681 aggcttcttg aagtcgctgt cccttaagtg agggtcgacg gtaccgcggg cccgggatcc
     4741 accggatcta gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct
     4801 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     4861 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     4921 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     4981 tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     5041 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     5101 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     5161 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     5221 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     5281 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     5341 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     5401 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     5461 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5521 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
     5581 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     5641 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5701 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5761 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5821 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5881 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
     5941 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     6001 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     6061 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     6121 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     6181 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     6241 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     6301 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     6361 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     6421 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     6481 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     6541 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     6601 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     6661 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     6721 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     6781 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     6841 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     6901 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
     6961 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     7021 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     7081 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
     7141 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
     7201 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
     7261 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     7321 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     7381 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7441 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7501 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     7561 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7621 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7681 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7741 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7801 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7861 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7921 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7981 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     8041 cctgattctg tggataaccg tattaccgcc atgcat
//
