LOCUS       Exported                7344 bp DNA     circular SYN 07-JUN-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 7, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        51..627
                     /label=CMV_immearly_promoter
                     /note="color: #85dae9"
     misc_feature    130..417
                     /label=CAG_enhancer
                     /note="color: #ffef86"
     promoter        585..654
                     /label=CMV_promoter
                     /note="color: #b4abac"
     promoter        597..716
                     /label=CMV2_promoter
                     /note="color: #ff9ccd"
     gene            729..1682
                     /label=SOX2
                     /note="color: #b7e6d7; direction: RIGHT"
     misc_feature    1689..1743
                     /label=T2A
                     /note="color: #d6b295"
     gene            1749..2693
                     /label=miRFP670
                     /note="color: #f58a5e; direction: RIGHT"
     terminator      2717..2987
                     /label=TK_PA_terminator
                     /note="color: #f8d3a9"
     misc_feature    3041..3347
                     /label=f1_origin
                     /note="color: #c7b0e3"
     promoter        3479..3747
                     /label=SV40_promoter
                     /note="color: #b4abac"
     gene            3865..4653
                     /label=NeoR/KanR
                     /note="color: #75c6a9; direction: RIGHT"
     terminator      4835..4954
                     /label=SV40_PA_terminator
                     /note="color: #ff9ccd"
     misc_feature    complement(5389..6008)
                     /label=pBR322_origin
                     /note="color: #ffef86; direction: LEFT"
     gene            complement(6163..7023)
                     /label=Ampicillin
                     /note="color: #f58a5e; direction: LEFT"
     promoter        complement(7065..7093)
                     /label=AmpR_promoter
                     /note="color: #b1ff67; direction: LEFT"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actctagaat gtacaacatg atggagacgg agctgaagcc gccgggcccg cagcaaactt
      781 cggggggcgg cggcggcaac tccaccgcgg cggcggccgg cggcaaccag aaaaacagcc
      841 cggaccgcgt caagcggccc atgaatgcct tcatggtgtg gtcccgcggg cagcggcgca
      901 agatggccca ggagaacccc aagatgcaca actcggagat cagcaagcgc ctgggcgccg
      961 agtggaaact tttgtcggag acggagaagc ggccgttcat cgacgaggct aagcggctgc
     1021 gagcgctgca catgaaggag cacccggatt ataaataccg gccccggcgg aaaaccaaga
     1081 cgctcatgaa gaaggataag tacacgctgc ccggcgggct gctggccccc ggcggcaata
     1141 gcatggcgag cggggtcggg gtgggcgccg gcctgggcgc gggcgtgaac cagcgcatgg
     1201 acagttacgc gcacatgaac ggctggagca acggcagcta cagcatgatg caggaccagc
     1261 tgggctaccc gcagcacccg ggcctcaatg cgcacggcgc agcgcagatg cagcccatgc
     1321 accgctacga cgtgagcgcc ctgcagtaca actccatgac cagctcgcag acctacatga
     1381 acggctcgcc cacctacagc atgtcctact cgcagcaggg cacccctggc atggctcttg
     1441 gctccatggg ttcggtggtc aagtccgagg ccagctccag cccccctgtg gttacctctt
     1501 cctcccactc cagggcgccc tgccaggccg gggacctccg ggacatgatc agcatgtatc
     1561 tccccggcgc cgaggtgccg gaacccgccg cccccagcag acttcacatg tcccagcact
     1621 accagagcgg cccggtgccc ggcacggcca ttaacggcac actgcccctc tcacacatgt
     1681 tggctagcga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc
     1741 ctggtaccgt agcaggtcat gcctctggca gccccgcatt cgggaccgcc tctcattcga
     1801 attgcgaaca tgaagagatc cacctcgccg gctcgatcca gccgcatggc gcgcttctgg
     1861 tcgtcagcga acatgatcat cgcgtcatcc aggccagcgc caacgccgcg gaatttctga
     1921 atctcggaag cgtactcggc gttccgctcg ccgagatcga cggcgatctg ttgatcaaga
     1981 tcctgccgca tctcgatccc accgccgaag gcatgccggt cgcggtgcgc tgccggatcg
     2041 gcaatccctc tacggagtac tgcggtctga tgcatcggcc tccggaaggc gggctgatca
     2101 tcgaactcga acgtgccggc ccgtcgatcg atctgtcagg cacgctggcg ccggcgctgg
     2161 agcggatccg cacggcgggt tcactgcgcg cgctgtgcga tgacaccgtg ctgctgtttc
     2221 agcagtgcac cggctacgac cgggtgatgg tgtatcgttt cgatgagcaa ggccacggcc
     2281 tggtattctc cgagtgccat gtgcctgggc tcgaatccta tttcggcaac cgctatccgt
     2341 cgtcgactgt cccgcagatg gcgcggcagc tgtacgtgcg gcagcgcgtc cgcgtgctgg
     2401 tcgacgtcac ctatcagccg gtgccgctgg agccgcggct gtcgccgctg accgggcgcg
     2461 atctcgacat gtcgggctgc ttcctgcgct cgatgtcgcc gtgccatctg cagttcctga
     2521 aggacatggg cgtgcgcgcc accctggcgg tgtcgctggt ggtcggcggc aagctgtggg
     2581 gcctggttgt ctgtcaccat tatctgccgc gcttcatccg tttcgagctg cgggcgatct
     2641 gcaaacggct cgccgaaagg atcgcgacgc ggatcaccgc gcttgagagc taaaccggtt
     2701 agtaatgagt ttaaacgggg gaggctaact gaaacacgga aggagacaat accggaagga
     2761 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt
     2821 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     2881 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     2941 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcag atctgcgcag
     3001 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     3061 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     3121 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     3181 catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     3241 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     3301 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     3361 ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa
     3421 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     3481 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     3541 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3601 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     3661 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     3721 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     3781 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     3841 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     3901 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     3961 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     4021 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     4081 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     4141 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     4201 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     4261 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     4321 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     4381 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     4441 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     4501 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     4561 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     4621 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat
     4681 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     4741 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     4801 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     4861 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     4921 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     4981 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     5041 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     5101 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     5161 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     5221 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     5281 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     5341 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     5401 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5461 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5521 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5581 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5641 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     5701 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5761 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5821 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     5881 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5941 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6001 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6061 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6121 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     6181 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     6241 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     6301 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     6361 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     6421 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     6481 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     6541 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     6601 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     6661 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     6721 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6781 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6841 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6901 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     6961 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7021 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7081 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     7141 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtcgactctc
     7201 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     7261 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     7321 aattgcatga agaatctgct tagg
//
