LOCUS       Exported                5411 bp DNA     circular SYN 30-MAY-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Camilla Ciolli Mattioli
  TITLE     Direct Submission
  JOURNAL   Exported Jun 7, 2022 from SnapGene 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5411
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(941..1801)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1916..1935
                     /label=46_gfp_FW
     misc_feature    2029..2497
                     /label=rpsM_promoter
     primer_bind     2029..2048
                     /label=465_rpsM_FW_leia
     misc_feature    2498..2632
                     /label=rpsM_gene
     misc_feature    2498..2500
                     /label=rpsM_START
     primer_bind     2615..2651
                     /label=101_GFP_FW
     primer_bind     2645..2678
                     /label=123_rpsB_GFP_FW
     misc_feature    2660..3373
                     /label=GFP
     CDS             2660..3373
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     2660..2683
                     /label=125_GFP_pKD3_FW
     primer_bind     2660..2683
                     /label=111_pBAD_GFP_FW
     primer_bind     complement(2859..2881)
                     /label=47_gfp_REV
     primer_bind     complement(3351..3373)
                     /label=126_GFP_pKD3_REV
     primer_bind     complement(3352..3431)
                     /label=102_GFP-AAV_REV
     primer_bind     complement(3352..3431)
                     /label=103_GFP-LVA_REV
     primer_bind     complement(3352..3431)
                     /label=104_GFP-LAA_REV
     primer_bind     complement(3416..3439)
                     /label=112_pBAD_GFP_REV
     oriT            4022..4109
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     1981 gtatcacgag gccctttcgt cttcaagaat tcgagctcgg tacccgggga tcccggagtt
     2041 catgcgtgat gcaatgaaag tgccgttcta cttcggtggg acctcactgc ttatcgttgt
     2101 tgtcgtgatt atggacttta tggctcaagt gcaaactctg atgatgtcca gtcagtatga
     2161 gtctgcattg aagaaggcga acctgaaagg ctacggccgt taattggtcg cctgagaagt
     2221 tacggagagt aaaaatgaaa gttcgtgctt ccgtcaagaa attatgccgt aactgcaaaa
     2281 tcgttaagcg tgatggtgtc atccgtgtga tttgcagtgc cgagccgaag cataaacagc
     2341 gccaaggctg attttttcgc atatttttct tgcaaagttg ggttgagctg gctagattag
     2401 ccagccaatc ttttgtatgt ctgtacgttt ccatttgagt atcctgaaaa cgggcttttc
     2461 agcatggtac gtacatatta aatagtagga gtgcatagtg gcccgtatag caggcattaa
     2521 cattcctgat cagaaacacg ccgtgatcgc gttaacttcg atctacggtg tcggcaagac
     2581 ccgttctaaa gccatcctgg ctgcagcggg tatcgctgaa aatgttaaga tcctctagat
     2641 ttaagaagga gatatacata tgagtaaagg agaagaactt ttcactggag ttgtcccaat
     2701 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
     2761 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
     2821 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
     2881 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
     2941 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
     3001 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
     3061 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
     3121 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
     3181 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
     3241 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
     3301 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
     3361 tgaactatac aaaaggcctg cagcaaacga cgaaaactac gctttagtag cttaaagctt
     3421 taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg tatgaaatct
     3481 aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg
     3541 gttatgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag catcgccagt
     3601 cactatggcg tgctgctagc gcttttagcc gctttagcgg cctttccccc tacccgaagg
     3661 gtgggggcgc gtgtgcagcc ccgcagggcc tgtctcggtc gatcattcag cccggctcat
     3721 ccttctggcg tggcggcaga ccgaacaagg cgcggtcgtg gtcgcgttca aggtacgcat
     3781 ccattgccgc catgagccga tcctccggcc actcgctgct gttcaccttg gccaaaatca
     3841 tggcccccac cagcaccttg cgccttgttt cgttcttgcg ctcttgctgc tgttcccttg
     3901 cccgcacccg ctgaatttcg gcattgattc gcgctcgttg ttcttcgagc ttggccagcc
     3961 gatccgccgc cttgttgctc cccttaacca tcttgacacc ccattgttaa tgtgctgtct
     4021 cgtaggctat catggaggca cagcggcggc aatcccgacc ctactttgta ggggaggggg
     4081 cacttaccgg tttctcttcg agaaactggc ctaacggcca cccttcgggc ggtgcgctct
     4141 ccgagggcca ttgcatggag ccgaaaagca aaagcaacag cgaggcagca tggcgattta
     4201 tcaccttacg gcgaaaaccg gcagcaggtc gggcggccaa tcggccaggg ccaaggccga
     4261 ctacatccag cgcgaaggca agtatgcccg cgacatggat gaagtcttgc acgccgaatc
     4321 cgggcacatg ccggagttcg tcgagcggcc cgccgactac tgggatgctg ccgacctgta
     4381 tgaacgcgcc aatgggcggc tgttcaagga ggtcgaattt gccctgccgg tcgagctgac
     4441 cctcgaccag cagaaggcgc tggcgtccga gttcgcccag cacctgaccg gtgccgagcg
     4501 cctgccgtat acgctggcca tccatgccgg tggcggcgag aacccgcact gccacctgat
     4561 gatctccgag cggatcaatg acggcatcga gcggcccgcc gctcagtggt tcaagcggta
     4621 caacggcaag accccggaga agggcggggc acagaagacc gaagcgctca agcccaaggc
     4681 atggcttgag cagacccgcg aggcatgggc cgaccatgcc aaccgggcat tagagcgggc
     4741 tggccacgac gcccgcattg accacagaac acttgaggcg cagggcatcg agcgcctgcc
     4801 cggtgttcac ctggggccga acgtggtgga gatggaaggc cggggcatcc gcaccgaccg
     4861 ggcagacgtg gccctgaaca tcgacaccgc caacgcccag atcatcgact tacaggaata
     4921 ccgggaggca atagaccatg aacgcaatcg acagagtgaa gaaatccaga ggcatcaacg
     4981 agttagcgga gcagatcgaa ccgctggccc agagcatggc gacactggcc gacgaagccc
     5041 ggcaggtcat gagccagacc cagcaggcca gcgaggcgca ggcggcggag tggctgaaag
     5101 cccagcgcca gacaggggcg gcatgggtgg agctggccaa agagttgcgg gaggtagccg
     5161 ccgaggtgag cagcgccgcg cagagcgccc ggagcgcgtc gcgggggtgg cactggaagc
     5221 tatggctaac cgtgatgctg gcttccatga tgcctacggt ggtgctgctg atcgcatcgt
     5281 tgctcttgct cgacctgacg ccactgacaa ccgaggacgg ctcgatctgg ctgcgcttgg
     5341 tggcccgatg aagaacgaca ggactttgca ggccataggc cgacagctca aggccatggg
     5401 ctgtgagcgc t
//
