LOCUS       Exported                9243 bp DNA     circular SYN 30-MAY-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Camilla Ciolli Mattioli
  TITLE     Direct Submission
  JOURNAL   Exported Jun 7, 2022 from SnapGene 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9243
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     mobile_element  152..317
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     primer_bind     413..452
                     /label=208
     primer_bind     413..433
                     /label=206
     promoter        complement(434..472)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     434..452
                     /label=207
     primer_bind     466..497
                     /label=204_intoAfeI_pBAD_FW_flipped
     primer_bind     473..497
                     /label=203_intoAfeI_pBAD_REV
     terminator      491..518
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      610..696
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1015..1731)
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1061..1095)
                     /label=460
     primer_bind     1078..1113
                     /label=461
     RBS             1740..1748
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(1775..2059)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(2033..2079)
                     /label=202_intoAfeI_pBAD_FW
     primer_bind     complement(2033..2059)
                     /label=205_intoAfeI_pBAD_REV_flipped
     CDS             2086..2964
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     primer_bind     3183..3227
                     /label=203_intoAfeI_pBAD_REV
     primer_bind     3183..3203
                     /label=205_intoAfeI_pBAD_REV_flipped
     primer_bind     3200..3227
                     /label=204_intoAfeI_pBAD_FW_flipped
     terminator      3221..3248
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      3340..3426
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3657..4367)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     RBS             4376..4384
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(4411..4695)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(4669..4716)
                     /label=202_intoAfeI_pBAD_FW
     primer_bind     complement(4669..4698)
                     /label=205_intoAfeI_pBAD_REV_flipped
     primer_bind     complement(4695..4716)
                     /label=204_intoAfeI_pBAD_FW_flipped
     primer_bind     complement(4696..4737)
                     /label=209
     primer_bind     complement(4696..4716)
                     /label=206
     primer_bind     complement(4717..4737)
                     /label=207
     primer_bind     complement(4861..4894)
                     /label=65_for-gibson-in-p5
     protein_bind    complement(4991..5038)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(5038..5059)
                     /label=43_p2_sgRNA-mut_REV
     primer_bind     5095..5114
                     /label=473_kana_FW
     promoter        5115..5143
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             5254..6048
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5308..5327)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5918..5937
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6052..6079)
                     /label=474_kana_REV
     protein_bind    complement(6100..6147)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      6198..6284
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6387..6481
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(6509..6525)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6510..6526)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    6530..6728
                     /label=Tn7R
     primer_bind     complement(6940..6959)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7059..7081
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7119..7137)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7205..7309
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7238..7261)
                     /label=207
     primer_bind     complement(7238..7256)
                     /label=208
     CDS             7310..8170
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7528..7547)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8471..8859
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            9128..9236
                     /note="incP origin of transfer"
ORIGIN
        1 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcgg gtcagtgagc gaggaagcgg
       61 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagcg
      121 cccaaacata acaggaagaa aaatgccccg ctgtgggcgg acaaaatagt tgggaactgg
      181 gaggggtgga aatggagttt ttaaggatta tttagggaag agtgacaaaa tagatgggaa
      241 ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg acaaaatagt ttggaactag
      301 atttcactta tctggttctt gaggcggtta aaagagccgt actcttctcc gatgtcgact
      361 aggccatgat gctcattctg tgggaccaaa acgaaaaaac accctttcgg gtgtcttttc
      421 tggaatttgg tacgcggata catatttgaa tgtatttaga aaaataaaca aaagagtttg
      481 tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttaatttgat gcctggcagt
      541 ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcgcaacgt tcaaatccgc
      601 tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
      661 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg
      721 catggggaga ccccacacta ccatcggcgc tacggcgttt cacttctgag ttcggcatgg
      781 ggtcaggtgg gaccaccgcg ctactgccgc caggcaaatt ctgttttatc agaccgcttc
      841 tgcgttctga tttaatctgt atcaggctga aaatcttctc tcatccgcca aaacagccaa
      901 gcttgcatgc ctgcaggtcg actctagtga taaactaccg cattaaagct tgcatgcctg
      961 caggtctgga catttttttt tttttttttt tttttttttt ttttatgtcc gcctttattt
     1021 gtatagttca tccatgccat gtgtaatccc agcagctgtt acaaactcaa gaaggaccat
     1081 gtggtctctc ttttcgttgg gatctttcga aagggcagat tgtgtggaca ggtaatggtt
     1141 gtctggtaaa aggacagggc catcgccaat tggagtattt tgttgataat ggtctgctag
     1201 ttgaacgctt ccatcttcaa tgttgtgtct aattttgaag ttaactttga ttccattctt
     1261 ttgtttgtct gccatgatgt atacattgtg tgagttatag ttgtattcca atttgtgtcc
     1321 aagaatgttt ccatcttctt taaaatcaat accttttaac tcgattctat taacaagggt
     1381 atcaccttca aacttgactt cagcacgtgt cttgtagttc ccgtcatctt tgaaaaatat
     1441 agttctttcc tgtacataac cttcgggcat ggcactcttg aaaaagtcat gctgtttcat
     1501 atgatctggg tatctcgcaa agcattgaac accataaccg aaagtagtga caagtgttgg
     1561 ccatggaaca ggtagttttc cagtagtgca aataaattta agggtaagtt ttccgtatgt
     1621 tgcatcacct tcaccctctc cactgacaga aaatttgtgc ccattaacat caccatctaa
     1681 ttcaacaaga attgggacaa ctccagtgaa aagttcttct cctttactca ttttttataa
     1741 cctccttacc tcctgctagc ccaaaaaaac gggtatggag aaacagtaga gagttgcgat
     1801 aaaaagcgtc aggtaggatc cgctaatctt atggataaaa atgctatggc atagcaaagt
     1861 gtgacgccgt gcaaataatc aatgtggact tttctgccgt gattatagac acttttgtta
     1921 cgcgtttttg tcatggcttt ggtcccgctt tgttacagaa tgcttttaat aagcggggtt
     1981 accggtttgg ttagcgagaa gagccagtaa aagacgcagt gacggcaatg tctgatgcaa
     2041 tatggacaat tggtttcttc tctgaatggc gggagtatga aaagtatggc tgaagcgcaa
     2101 aatgatcccc tgctgccggg atactcgttt aatgcccatc tggtggcggg tttaacgccg
     2161 attgaggcca acggttatct cgattttttt atcgaccgac cgctgggaat gaaaggttat
     2221 attctcaatc tcaccattcg cggtcagggg gtggtgaaaa atcagggacg agaatttgtt
     2281 tgccgaccgg gtgatatttt gctgttcccg ccaggagaga ttcatcacta cggtcgtcat
     2341 ccggaggctc gcgaatggta tcaccagtgg gtttactttc gtccgcgcgc ctactggcat
     2401 gaatggctta actggccgtc aatatttgcc aatacggggt tctttcgccc ggatgaagcg
     2461 caccagccgc atttcagcga cctgtttggg caaatcatta acgccgggca aggggaaggg
     2521 cgctattcgg agctgctggc gataaatctg cttgagcaat tgttactgcg gcgcatggaa
     2581 gcgattaacg agtcgctcca tccaccgatg gataatcggg tacgcgaggc ttgtcagtac
     2641 atcagcgatc acctggcaga cagcaatttt gatatcgcca gcgtcgcaca gcatgtttgc
     2701 ttgtcgccgt cgcgtctgtc acatcttttc cgccagcagt tagggattag cgtcttaagc
     2761 tggcgcgagg accaacgtat cagccaggcg aagctgcttt tgagcaccac ccggatgcct
     2821 atcgccaccg tcggtcgcaa tgttggtttt gacgatcaac tctatttctc gcgggtattt
     2881 aaaaaatgca ccggggccag cccgagcgag ttccgtgccg gttgtgaaga aaaagtgaat
     2941 gatgtagccg tcaagttgtc ataattggta acgaatcaga caattgacgg cttgacggag
     3001 tagcataggg tttgcagaat ccctgcttcg tccatttgac aggcacatta tgcatcgatg
     3061 ataagctgtc aaacatgagc agatcctcta cgccggacgc atcgtggccg gcatcaccgg
     3121 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
     3181 tcgccacttc gggctcatga gcagagtttg tagaaacgca aaaaggccat ccgtcaggat
     3241 ggccttctgc ttaatttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc
     3301 gggccgttgc ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
     3361 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt
     3421 tatttgatgc ctggcagttc cctactctcg catggggaga ccccacacta ccatcggcgc
     3481 tacggcgttt cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg ctactgccgc
     3541 caggcaaatt ctgttttatc agaccgcttc tgcgttctga tttaatctgt atcaggctga
     3601 aaatcttctc tcatccgcca aaacagccaa gcttgcatgc ctgcaggtcg actctattac
     3661 ttgtacagct cgtccatgcc gccggtggag tggcggccct cggcgcgttc gtactgttcc
     3721 acgatggtgt agtcctcgtt gtgggaggtg atgtccaact tgatgttgac gttgtaggcg
     3781 ccgggcagct gcacgggctt cttggccttg taggtggtct tgacctcagc gtcgtagtgg
     3841 ccgccgtcct tcagcttcag cctctgcttg atctcgccct tcagggcgcc gtcctcgggg
     3901 tacatccgct cggaggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg
     3961 gaggggaagt tggtgccgcg cagcttcacc ttgtagatga actcgccgtc ctgcagggag
     4021 gagtcctggg tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg
     4081 aagccctcgg ggaaggacag cttcaagtag tcggggatgt cggcggggtg cttcacgtag
     4141 gccttggagc cgtacatgaa ctgaggggac aggatgtccc aggcgaaggg cagggggcca
     4201 cccttggtca ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg
     4261 ccctcgatct cgaactcgtg gccgttcacg gagccctcca tgtgcacctt gaagcgcatg
     4321 aactccttga tgatggccat gttatcctcc tcgcccttgc tcaccatttt ttataacctc
     4381 cttacctcct gctagcccaa aaaaacgggt atggagaaac agtagagagt tgcgataaaa
     4441 agcgtcaggt aggatccgct aatcttatgg ataaaaatgc tatggcatag caaagtgtga
     4501 cgccgtgcaa ataatcaatg tggacttttc tgccgtgatt atagacactt ttgttacgcg
     4561 tttttgtcat ggctttggtc ccgctttgtt acagaatgct tttaataagc ggggttaccg
     4621 gtttggttag cgagaagagc cagtaaaaga cgcagtgacg gcaatgtctg atgcaatatg
     4681 gacaattggt ttcttgcttg tttcggcgtg ggtatgaatt catgtggctg accgttctgt
     4741 tgtctctcgc tcttccgagt agacgaacaa taaggcctcc ctaacggggg gcctttttta
     4801 ttgataacaa aagtcagtgc ttccgctatt tccaaaatac cgggctaata cggtttaaac
     4861 gacctcctgg atttgctcag acagcctttt cgtcattcgt ttcagccaaa aaacttaaga
     4921 ccgccggtct tgtccactac cttgcagtaa tgcggtggac aggatcggcg gttttctttt
     4981 ctcttctcaa gaagttccta tactttctag agaataggaa cttcggaata ggaacttcct
     5041 cctgaacggc cataagaacg aaggctgtct gttgaactct cgagccgtgg aaacggatga
     5101 aggcacgaac ccagttgaca taagcctgtt cggttcgtaa actgtaatgc aagtagcgta
     5161 tgcgctcaaa actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaaga
     5221 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     5281 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     5341 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     5401 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     5461 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     5521 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     5581 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5641 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5701 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5761 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     5821 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5881 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5941 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     6001 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     6061 ggttcgaaat gactcgagca tcagcttcaa aagcgctctg aagttcctat actttctaga
     6121 gaataggaac ttcggaatag gtacttcaag atccccaatt cgagctcatc gtccgggccg
     6181 caagctccta gcggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     6241 aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct caagctagag
     6301 agtcattacc ccaggcgttt aagggcacca ataactgcct taaaaaaatt acgccccgcc
     6361 ctgccactca tcgcagtcta gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     6421 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     6481 ccaagctcag ctaattaagc tagcttatcg ataccgtcga cctcgaaccc cacgcccctc
     6541 tttaatacga cgggcaattt gcacttcaga aaatgaagag tttgctttag ccataacaaa
     6601 agtccagtat gctttttcac agcataactg gactgatttc agtttacaac tattctgtct
     6661 agtttaagac tttattgtca tagtttagat ctattttgtt cagtttaaga ctttattgtc
     6721 cgcccacacc tgcttacgca gggcatccat ttattactca accgtaaccg attttgccag
     6781 gttacgcggc tggtcctcta gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     6841 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     6901 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     6961 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     7021 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     7081 gttttcaccg tcgtcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     7141 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     7201 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7261 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7321 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7381 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     7441 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     7501 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     7561 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     7621 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     7681 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     7741 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     7801 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     7861 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     7921 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     7981 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtccc gcggtatcat
     8041 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8101 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8161 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8221 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8281 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggcagtta
     8341 ttggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     8401 agattgattt aaaacttcat ttttaatttt tgcggccgca agatccggcc acgatgcgtc
     8461 cggcgtagag gatctgaaga tcagcagttc aacctgttga tagtacgtac taagctctca
     8521 tgtttcacgt actaagctct catgtttaac gtactaagct ctcatgttta acgaactaaa
     8581 ccctcatggc taacgtacta agctctcatg gctaacgtac taagctctca tgtttcacgt
     8641 actaagctct catgtttgaa caataaaatt aatataaatc agcaacttaa atagcctcta
     8701 aggttttaag ttttataaga aaaaaaagaa tatataaggc ttttaaagct tttaaggttt
     8761 aacggttgtg gacaacaagc cagggatgta acgcactgag aagcccttag agcctctcaa
     8821 agcaattttg agtgacacag gaacacttaa cggctgacat gggaattagc catggcatca
     8881 cagtatcgtg atgacagagg cagggagtgc ggccttttta cggttcctgg ccttttgctg
     8941 gccttttgct cacatgttct ttcctgcgtt atcaggggat tccttaaggt atactttccg
     9001 ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg
     9061 cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc
     9121 gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg
     9181 tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccgataa
     9241 gct
//
