LOCUS       Exported               10267 bp DNA     circular SYN 12-MAR-2022
DEFINITION  .
ACCESSION   -
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 8, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
COMMENT     Imported From
            Path: C:\Users\DCLin-workstation\Downloads
            Filename: TL146_lenti_TRE_GFP-PAK4cat.gb
FEATURES             Location/Qualifiers
     source          1..10267
                     /lab_host="."
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2602..3129
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          2811..3125
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     misc_feature    254..817
                     /label=CMV promoter
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    772..792
                     /label=CMV_fwd_primer
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     LTR             835..1015
                     /label=5'LTR
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=pink"
                     /note="color: #ffe4c4; direction: RIGHT"
     misc_feature    956..1074
                     /label=Primer binding site (PBS)
                     /note="/ApEinfo_fwdcolor=#ff8000"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1075..1187
                     /label=Psi packaging signal
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1125..1126
                     /label=splice donor (SD)
                     /note="/ApEinfo_fwdcolor=#ffff00"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1620..1974
                     /label=Rev Response Element (RRE)
                     /note="/ApEinfo_fwdcolor=#ff80ff"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2284..2293
                     /label=splice acceptor? (SA)
                     /note="/ApEinfo_fwdcolor=#ffff00"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2432..2464
                     /label=central polypurine tract (cPPT)
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     2574..2626
                     /label=TL053A_F
                     /note="color: black; sequence: 
                     ACAGCAGAGATCCAGTTTGGTTAATTAACCATTCGCCATTCAGGCTGCGCAAC"
     misc_feature    2594..2601
                     /label=PacI
                     /note="/ApEinfo_fwdcolor=#ff0000"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     2728..2750
                     /label=M13/pUC Forward
                     /note="color: black; sequence: CCAGTCACGACGTTGTAAAACGA"
     primer_bind     2742..2759
                     /label=M13 Forward
                     /note="color: black; sequence: GTAAAACGACGGCCAGTG"
     primer_bind     2743..2759
                     /label=M13 fwd
                     /note="color: black; sequence: TAAAACGACGGCCAGTG"
     promoter        2768..2786
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     2769..2788
                     /label=T7
                     /note="color: black; sequence: AATACGACTCACTATAGGGC"
     promoter        2811..3125
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of 
                     seventet operator sequences followed by the minimal 
                     CMVpromoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    2819..2837
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b; direction: RIGHT"
     protein_bind    2855..2873
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b; direction: RIGHT"
     protein_bind    2890..2908
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b; direction: RIGHT"
     protein_bind    2926..2944
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b; direction: RIGHT"
     protein_bind    2962..2980
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b; direction: RIGHT"
     protein_bind    2997..3015
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b; direction: RIGHT"
     protein_bind    3033..3051
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b; direction: RIGHT"
     primer_bind     3099..3123
                     /label=LNCX
                     /note="color: black; sequence: GCTCGTTTAGTGAACCGTCAGATCG"
     primer_bind     3111..3165
                     /label=TL146_F_v2
                     /note="color: black; sequence: 
                     AACCGTCAGATCGCCTGGAACCGGTGCCACCATGAGTAAAGGAGAAGAACTTTTC"
     misc_feature    3130..3135
                     /label=AgeI site
                     /note="/created_by=xiuyuanli"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3136..3141
                     /label=kozak
                     /note="/created_by=xiuyuanli"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3142..4932
                     /label=gfp-ibox-pak4
                     /note="Geneious type: ORF"
                     /note="/modified_by=xiuyuanli"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             3142..3855
                     /codon_start=1
                     /transl_table=0
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal ofa
                     cryptic intron"
                     /note="color: #993366"
                     /translation="MSKGEELFTGVVPILVELDGDVNGYKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDDPVLLPDNHYLSTQSALSKDPNEKRDHMVILE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(3568..3589)
                     /label=seq_mgfp5_r1
                     /note="color: black; sequence: CGTTGTGGGAGTTGTAGTTGTA"
     misc_feature    3862..3867
                     /label=BamHI
                     /note="/created_by=xiuyuanli"
                     /note="/modified_by=xiuyuanli"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             3868..3981
                     /codon_start=1
                     /transl_table=0
                     /label=INKA-box
                     /note="color: #993366"
                     /translation="EAEDWTAALLNRGRSRQPLVLGDNCFADLVHNWMELPE"
     CDS             3988..4929
                     /codon_start=1
                     /transl_table=0
                     /label=Pak4cat
                     /note="color: #993366"
                     /translation="PAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLD
                     NFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEM
                     YNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRD
                     IKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIW
                     SLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPA
                     QRATAAELLKHPFLAKAGPPASIVPLMRQNRTR"
     primer_bind     complement(4258..4279)
                     /label=seq_Pak4cat_r1
                     /note="color: black; sequence: GCTGGTAGTCCCTCATGATTAC"
     primer_bind     complement(4923..4960)
                     /label=TL145_R
                     /note="color: black; sequence: 
                     TTATCGATAAGCTTGATATCGCGGCCGCTCATCTGGTG"
     misc_feature    4933..4940
                     /label=NotI
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=pink"
                     /note="/modified_by=xiuyuanli"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4955..5551
                     /label=WPRE
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(5012..5032)
                     /label=WPRE-R
                     /note="color: black; sequence: CATAGCGTAAAAGGAGCAACA"
     misc_feature    5724..5755
                     /label=polypurine tract (PPT)
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    6072..6252
                     /label=3' LTR
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    6284..6508
                     /label=BGH polyadenylation sequence
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    6571..6877
                     /label=f1_origin
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    6991..7011
                     /label=pBABE_3_primer
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    6997..7212
                     /label=SV40_enhancer
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    7009..7277
                     /label=SV40_promoter
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    7176..7253
                     /label=SV40_origin
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    7238..7257
                     /label=SV40pro_F_primer
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    7371..7438
                     /label=EM7_promoter
                     /note="/ApEinfo_fwdcolor=#8080c0"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    7439..7813
                     /label=Sh ble (Zeocin Resistance)
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    7946..8065
                     /label=SV40_PA_terminator
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    8109..8127
                     /label=M13_reverse_primer
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    8500..9119
                     /label=pBR322 origin
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    9274..10134
                     /label=Ampicllin Resistance (Rev-Com)
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    10176..10204
                     /label=Ampicillin Promoter
                     /note="/ApEinfo_fwdcolor=#80ffff"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}"
                     /note="/ApEinfo_revcolor=green"
                     /note="color: #a6acb3; direction: RIGHT"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta accattcgcc attcaggctg cgcaactgtt gggaagggcg
     2641 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     2701 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     2761 gcgcgcgtaa tacgactcac tatagggcga attggctttc gtcttcactc gagtttactc
     2821 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgatgt
     2881 cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
     2941 gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttatccc
     3001 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
     3061 gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga
     3121 tcgcctggaa ccggtgccac catgagtaaa ggagaagaac ttttcactgg agttgtccca
     3181 attcttgttg aattagatgg tgatgttaat gggtacaaat tttctgtcag tggagagggt
     3241 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
     3301 cctgttccat ggccaacact tgtcactact ctcacttatg gtgttcaatg cttttcaaga
     3361 tatccagatc atatgaagcg gcacgacttc ttcaagagcg ccatgcctga gggatacgtg
     3421 caggagagga ccatcttctt caaggacgac gggaactaca agacacgtgc tgaagtcaag
     3481 tttgagggag acaccctcgt caacaggatc gagcttaagg gaatcgattt caaggaggac
     3541 ggaaacatcc tcggccacaa gttggaatac aactacaact cccacaacgt atacatcatg
     3601 gccgacaagc aaaagaacgg catcaaagcc aacttcaaga cccgccacaa catcgaagac
     3661 ggcggcgtgc aactcgctga tcattatcaa caaaatactc caattggcga tgaccctgtc
     3721 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
     3781 aagagagacc acatggtcat tcttgagttt gtaacggctg ctgggattac acatggcatg
     3841 gatgaactat acaaatcagg tggatccgaa gcagaggact ggacagcagc cctactgaac
     3901 aggggtcgca gtcgccagcc cctggtacta ggggacaatt gctttgctga cttggtgcac
     3961 aactggatgg agctgcctga ggaattcccc gccgcccctg ctgttcctgg gccccctggc
     4021 ccccgctcac cacagcggga gccacagcga gtatcccatg agcagttccg ggctgccctg
     4081 cagctggtgg tggacccagg cgacccccgc tcctacctgg acaacttcat caagattggc
     4141 gagggctcca cgggcatcgt gtgcatcgcc accgtgcgca gctcgggcaa gctggtggcc
     4201 gtcaagaaga tggacctgcg caagcagcag aggcgcgagc tgctcttcaa cgaggtggta
     4261 atcatgaggg actaccagca cgagaatgtg gtggagatgt acaacagcta cctggtgggg
     4321 gacgagctct gggtggtcat ggagttcctg gaaggaggcg ccctcaccga catcgtcacc
     4381 cacaccagga tgaacgagga gcagatcgca gccgtgtgcc ttgcagtgct gcaggccctg
     4441 tcggtgctcc acgcccaggg cgtcatccac cgggacatca agagcgactc gatcctgctg
     4501 acccatgatg gcagggtgaa gctgtcagac tttgggttct gcgcccaggt gagcaaggaa
     4561 gtgccccgaa ggaagtcgct ggtcggcacg ccctactgga tggccccaga gctcatctcc
     4621 cgccttccct acgggccaga ggtagacatc tggtcgctgg ggataatggt gattgagatg
     4681 gtggacggag agccccccta cttcaacgag ccacccctca aagccatgaa gatgattcgg
     4741 gacaacctgc caccccgact gaagaacctg cacaaggtgt cgccatccct gaagggcttc
     4801 ctggaccgcc tgctggtgcg agaccctgcc cagcgggcca cggcagccga gctgctgaag
     4861 cacccattcc tggccaaggc agggccgcct gccagcatcg tgcccctcat gcgccagaac
     4921 cgcaccagat gagcggccgc gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     4981 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     5041 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     5101 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     5161 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     5221 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     5281 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     5341 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     5401 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     5461 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     5521 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac
     5581 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     5641 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     5701 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     5761 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     5821 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     5881 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     5941 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     6001 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     6061 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6121 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6181 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6241 aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     6301 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     6361 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     6421 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     6481 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     6541 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     6601 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     6661 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     6721 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     6781 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     6841 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     6901 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6961 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     7021 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     7081 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     7141 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     7201 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     7261 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     7321 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     7381 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     7441 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
     7501 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
     7561 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
     7621 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
     7681 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
     7741 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
     7801 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
     7861 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     7921 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     7981 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8041 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     8101 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     8161 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     8221 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     8281 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     8341 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     8401 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     8461 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8521 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8581 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8641 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8701 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8761 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8821 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8881 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8941 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9001 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9061 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     9121 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     9181 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     9241 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     9301 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9361 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     9421 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     9481 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     9541 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     9601 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9661 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9721 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9781 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9841 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9901 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9961 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10021 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    10081 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    10141 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    10201 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    10261 acctgac
//
