LOCUS       ATP1A1_T804N_hPG        5382 bp    DNA     circular UNA 08-JUN-2022
DEFINITION  Ligation of 2 sequences.
ACCESSION   urn.local...x-ewzuvlk
VERSION     urn.local...x-ewzuvlk
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             complement(7..9)
                     /standard_name="Stop"
     misc_feature    complement(10..726)
                     /note="Geneious type: CDS"
                     /standard_name="EBFP"
     misc_feature    complement(526..528)
                     /standard_name="H66"
     misc_feature    complement(529..531)
                     /standard_name="S65"
     promoter        complement(778..1293)
                     /standard_name="hPGK1"
     misc_feature    1342..2086
                     /standard_name="ATP1A1_HA-R"
     promoter        2466..2530
                     /standard_name="AmpR promoter"
     CDS             2531..3388
                     /standard_name="AmpR"
     rep_origin      3562..4150
     misc_feature    4314..4333
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="pUC19 R"
     misc_feature    4392..5139
                     /standard_name="ATP1A1_HA-L"
     exon            4953..5107
                     /standard_name="Exon 17"
     misc_feature    5069..5071
                     /standard_name="T804N"
     terminator      complement(5156..5380)
                     /standard_name="bGH Poly A"
ORIGIN      
        1 tgcaggtcac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
       61 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct
      121 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
      181 gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt
      241 gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc
      301 cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg
      361 gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc
      421 cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc
      481 gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtggc tcagggtggt
      541 cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag
      601 cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac
      661 gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct
      721 caccatggct ccctgaaaat acagattctc ggcggccgcc ttaaggctga gggtacccct
      781 ggggagagag gtcggtgatt cggtcaacga gggagccgac tgccgacgtg cgctccggag
      841 gcttgcagaa tgcggaacac cgcgcgggca ggaacagggc ccacactacc gccccacacc
      901 ccgcctcccg caccgcccct tcccggccgc tgctctcggc gcgccctgct gagcagccgc
      961 tattggccac agcccatcgc ggtcggcgcg ctgccattgc tccctggcgc tgtccgtctg
     1021 cgagggtact agtgagacgt gcggcttccg tttgtcacgt ccggcacgcc gcgaaccgca
     1081 aggaaccttc ccgacttagg ggcggagcag gaagcgtcgc cggggggccc acaagggtag
     1141 cggcgaagat ccgggtgacg ctgcgaacgg acgtgaagaa tgtgcgagac ccagggtcgg
     1201 cgccgctgcg tttcccggaa ccacgcccag agcagccgcg tccctgcgca aacccagggc
     1261 tgccttggaa aaggcgcaac cccaaccccg tggatgcatt aaaaaacctc ccacacctcc
     1321 ccctgaacct gaaacaagct tgtgaggtgt gagctgtgtc ttcattcact ggcactatgc
     1381 tcccagcatc catgaggctg gcgttgggaa aagaaattct caggaccagt atccagtgtg
     1441 tgtcccaatc ccggcttcac agaatcagta ttaccactct tcaaggcagc agttaacatt
     1501 tgtttatcag atgggaatct ttatttttgt atacttcacc taaaagattt ttaaatggga
     1561 gggcaagaat tttataacaa aaggttcaca atattagctt ccttattttt agtaactaaa
     1621 ttccttctcc ccaccccttc ccaggttcct gccatctccc tggcttatga gcaggctgag
     1681 agtgacatca tgaagagaca gcccagaaat cccaaaacag acaaacttgt gaatgagcgg
     1741 ctgatcagca tggcctatgg gcagattggt aagctgcagc ctggagtggg aagctggcac
     1801 atctaaggca tctgaggtga tggtgtccac ctcaggttga ggttgatttc agagactgca
     1861 aatccaggcg actttcaggt ctaggatgag ccctaacgga gtgagcctgt ggagtttctc
     1921 tgaactctct tccatgtgag aatacatctg ttgctgtttg cccagttacc ttttgtggaa
     1981 actggttttc tatactgagc accttggaac tgggctcaac tctgcctgag aaatgaggat
     2041 gtccttcctc actgagcctt gcggaacagg aggcatggtt ctaagggcta gccggaagcg
     2101 ctcctccctc tgcgagagca atggctccgt ccggtttcga gccatatggt gcactctcag
     2161 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     2221 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     2281 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     2341 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     2401 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2461 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2521 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     2581 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     2641 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     2701 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2761 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     2821 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     2881 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     2941 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     3001 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     3061 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     3121 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     3181 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3241 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     3301 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3361 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     3421 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     3481 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3541 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3601 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3661 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     3721 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3781 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3841 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3901 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3961 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4021 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4081 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4141 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4201 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4261 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     4321 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     4381 atgcagaatt ccacccacca gagtcattac tttgagtagc tcttgttaat gctgggggct
     4441 atgtttgttg tcacttctca gttctgttat ttggtgtagg gcctgtgtga atacttgcct
     4501 gtgacggttc tcaggcttca taaatagtct caataggaaa ggagcagtgt ctgtaatgag
     4561 tgctcagtgg gggcatgcat cgcactattt ccatcgctag gaaaagtgat tggtattaac
     4621 ccgttttccc ttttctgggg tagggtgcta tcgtggctgt gactggtgac ggtgtgaatg
     4681 actctccagc tttgaagaaa gcagacattg gggttgctat ggggattgct ggctcagatg
     4741 tgtccaagca agctgctgac atgattcttc tggatgacaa ctttgcctca attgtgactg
     4801 gagtagagga aggtgagagc tatttaaggt gtacaccaag atcttattca gatactgccc
     4861 attagcatcc atttctgtat acttcttgga tatgttcagt ttccagtgtg cttgtctcat
     4921 aagctaacag taaaaaatct tggttttcat aggtcgtctg atctttgata acttgaagaa
     4981 atccattgct tataccttaa ccagtaacat tcccgagatc accccgttcc tgatatttat
     5041 tattgcaaac attccactac cactggggaa tgtcaccatc ctctgcattg acttgggcac
     5101 tgacatggtg agtgtcacaa cagtcacaga tcgatatcag acgtgatatg tcgacccata
     5161 gagcccaccg catccccagc atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc
     5221 tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga tgcaatttcc
     5281 tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga aggcacgggg
     5341 gaggggcaaa caacagatgg ctggcaacta gaaggcacag cc
//
