LOCUS       Exported                9915 bp DNA     circular SYN 09-JUN-2022
DEFINITION  -.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 8, 2016 from SnapGene 2.3.2
            http://www.snapgene.com
REFERENCE   2  (bases 1 to 9915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 9, 2022 from SnapGene 5.3.0
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exported 
            Nov 8, 2016 from SnapGene 2.3.2
            http://www.snapgene.com"
FEATURES             Location/Qualifiers
     source          1..9915
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        237..616
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..820
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        618..816
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early  
                     promoter"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus  
                     type 1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1685..1918
                     /label=RRE
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for  
                     Rev-dependent mRNA export from the nucleus to the  
                     cytoplasm."
     misc_feature    1685..1918
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2445..2562
                     /label=cPPT/CTS
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination  
                     sequence of HIV-1"
     misc_feature    2445..2562
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2625..2654
                     /label=pAF
     promoter        2667..2878
                     /label=EFS
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     2887..2916
                     /label=gAF
     primer_bind     complement(2887..2916)
                     /label=pAR
     CDS             2923..5370
                     /codon_start=1
                     /label=ACE2
                     /translation="MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSL
                     ASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNG
                     SSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLW
                     AWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQL
                     IEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSL
                     TVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQ
                     KAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEG
                     FHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEK
                     WRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYT
                     RTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGA
                     KNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEW
                     NDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDI
                     IPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVI
                     VVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSFGTETSQV
                     APA"
     misc_feature    complement(5376..5405)
                     /label=gAR
     misc_feature    5376..5405
                     /label=pBF
     promoter        5406..5910
                     /label=PGK
                     /note="/vntifkey=""29"""
     misc_feature    complement(5911..5940)
                     /label=pBR
     misc_feature    5911..5940
                     /label=gBF
     CDS             5947..6741
                     /codon_start=1
                     /label=Neo
                     /note="/vntifkey=""4"""
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    complement(6028..6766)
                     /label=seq wpre.rev
     misc_feature    complement(6752..6781)
                     /label=gBR
     misc_feature    6804..7392
                     /label=WPRE
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional  
                     regulatory element"
     misc_feature    6804..7392
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7395..7411)
                     /label=KS primer
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar  
                     variants"
     primer_bind     complement(7395..7411)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             7921..8101
                     /label=5' LTR (truncated)
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     LTR             7921..8101
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      8163..8751
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  
                     replication"
     rep_origin      complement(8163..8751)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8922..9782)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(8922..9782)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9783..9887)
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
     promoter        complement(9783..9887)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct ggaggaggag
     1561 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1621 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1681 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1741 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     1801 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1861 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1921 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1981 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     2041 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     2101 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     2161 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     2221 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     2281 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     2341 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     2401 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2461 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2521 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2581 agcagagatc cagtttggtt agtaccgggc ccgggtcgac tagtgatcag tgtgagggag
     2641 tgtaaagctg gtttaaaccc tgcagggggc agagcgcaca tcgcccacag tccccgagaa
     2701 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
     2761 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     2821 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagcg
     2881 ccggtgctaa ctcgaacgct agctgtgcga tcgtttgcca ccatgtcaag ctcttcctgg
     2941 ctccttctca gccttgttgc tgtaactgct gctcagtcca ccattgagga acaggccaag
     3001 acatttttgg acaagtttaa ccacgaagcc gaagacctgt tctatcaaag ttcacttgct
     3061 tcttggaatt ataacaccaa tattactgaa gagaatgtcc aaaacatgaa taatgctggg
     3121 gacaaatggt ctgccttttt aaaggaacag tccacacttg cccaaatgta tccactacaa
     3181 gaaattcaga atctcacagt caagcttcag ctgcaggctc ttcagcaaaa tgggtcttca
     3241 gtgctctcag aagacaagag caaacggttg aacacaattc taaatacaat gagcaccatc
     3301 tacagtactg gaaaagtttg taacccagat aatccacaag aatgcttatt acttgaacca
     3361 ggtttgaatg aaataatggc aaacagttta gactacaatg agaggctctg ggcttgggaa
     3421 agctggagat ctgaggtcgg caagcagctg aggccattat atgaagagta tgtggtcttg
     3481 aaaaatgaga tggcaagagc aaatcattat gaggactatg gggattattg gagaggagac
     3541 tatgaagtaa atggggtaga tggctatgac tacagccgcg gccagttgat tgaagatgtg
     3601 gaacatacct ttgaagagat taaaccatta tatgaacatc ttcatgccta tgtgagggca
     3661 aagttgatga atgcctatcc ttcctatatc agtccaattg gatgcctccc tgctcatttg
     3721 cttggtgata tgtggggtag attttggaca aatctgtact ctttgacagt tccctttgga
     3781 cagaaaccaa acatagatgt tactgatgca atggtggacc aggcctggga tgcacagaga
     3841 atattcaagg aggccgagaa gttctttgta tctgttggtc ttcctaatat gactcaagga
     3901 ttctgggaaa attccatgct aacggaccca ggaaatgttc agaaagcagt ctgccatccc
     3961 acagcttggg acctggggaa gggcgacttc aggatcctta tgtgcacaaa ggtgacaatg
     4021 gacgacttcc tgacagctca tcatgagatg gggcatatcc agtatgatat ggcatatgct
     4081 gcacaacctt ttctgctaag aaatggagct aatgaaggat tccatgaagc tgttggggaa
     4141 atcatgtcac tttctgcagc cacacctaag catttaaaat ccattggtct tctgtcaccc
     4201 gattttcaag aagacaatga aacagaaata aacttcctgc tcaaacaagc actcacgatt
     4261 gttgggactc tgccatttac ttacatgtta gagaagtgga ggtggatggt ctttaaaggg
     4321 gaaattccca aagaccagtg gatgaaaaag tggtgggaga tgaagcgaga gatagttggg
     4381 gtggtggaac ctgtgcccca tgatgaaaca tactgtgacc ccgcatctct gttccatgtt
     4441 tctaatgatt actcattcat tcgatattac acaaggaccc tttaccaatt ccagtttcaa
     4501 gaagcacttt gtcaagcagc taaacatgaa ggccctctgc acaaatgtga catctcaaac
     4561 tctacagaag ctggacagaa actgttcaat atgctgaggc ttggaaaatc agaaccctgg
     4621 accctagcat tggaaaatgt tgtaggagca aagaacatga atgtaaggcc actgctcaac
     4681 tactttgagc ccttatttac ctggctgaaa gaccagaaca agaattcttt tgtgggatgg
     4741 agtaccgact ggagtccata tgcagaccaa agcatcaaag tgaggataag cctaaaatca
     4801 gctcttggag ataaagcata tgaatggaac gacaatgaaa tgtacctgtt ccgatcatct
     4861 gttgcatatg ctatgaggca gtacttttta aaagtaaaaa atcagatgat tctttttggg
     4921 gaggaggatg tgcgagtggc taatttgaaa ccaagaatct cctttaattt ctttgtcact
     4981 gcacctaaaa atgtgtctga tatcattcct agaactgaag ttgaaaaggc catcaggatg
     5041 tcccggagcc gtatcaatga tgctttccgt ctgaatgaca acagcctaga gtttctgggg
     5101 atacagccaa cacttggacc tcctaaccag ccccctgttt ccatatggct gattgttttt
     5161 ggagttgtga tgggagtgat agtggttggc attgtcatcc tgatcttcac tgggatcaga
     5221 gatcggaaga agaaaaataa agcaagaagt ggagaaaatc cttatgcctc catcgatatt
     5281 agcaaaggag aaaataatcc aggattccaa aacactgatg atgttcagac ctcctttggt
     5341 accgagacct cccaggtggc gcccgcttaa tgtacaaacc gctgttccta ggaatcccga
     5401 ggcctctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     5461 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     5521 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     5581 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     5641 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     5701 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     5761 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
     5821 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     5881 ttctcctctt cctcatctcc gggcctttcg gacccgacat tagcgctaca gcttaagcgg
     5941 gccaccatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     6001 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     6061 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     6121 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     6181 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     6241 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     6301 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     6361 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     6421 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
     6481 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     6541 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     6601 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     6661 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     6721 cttcttgacg agttcttctg aggatccgtt taaacgttgt tgtttggggt tgaattactc
     6781 ttctagaccg cggcgcgccc gataatcaac ctctggatta caaaatttgt gaaagattga
     6841 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     6901 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     6961 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     7021 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     7081 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     7141 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     7201 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     7261 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7321 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7381 ccgcctcccc gcatcgatac cgtcgacctc gatcgagacc tagaaaaaca tggagcaatc
     7441 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     7501 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     7561 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     7621 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     7681 tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac
     7741 aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc
     7801 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg
     7861 aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact
     7921 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7981 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8041 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     8101 agcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8161 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8221 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8281 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8341 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8401 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8461 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8521 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8581 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     8641 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8701 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8761 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8821 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8881 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8941 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9001 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9061 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9121 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9181 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9241 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9301 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9361 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9421 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9481 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9541 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9601 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9661 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9721 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9781 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9841 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9901 aaagtgccac ctgac
//
