LOCUS       pOdd_spacer1            2260 bp ds-DNA     circular     22-MAR-2018
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        complement(1866..2013)
                     /locus_tag="specR_promoter_from_pKD13"
                     /label=specR_promoter_from_pKD13
                     /ApEinfo_fwdcolor="#a5ce0b"
                     /ApEinfo_revcolor="#a5ce0b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(395..983)
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
                     /locus_tag="pMB1-pUC19 ORI variant"
                     /label=pMB1-pUC19 ORI variant
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1510..1865)
                     /locus_tag="Spec gene"
                     /label=Spec gene
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="#ffc100"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1510..1511)
                     /locus_tag="BspMI"
                     /label=BspMI
                     /ApEinfo_fwdcolor="#ff1827"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2219..2260)
                     /locus_tag="In T-DNA region AH003392.2"
                     /label=In T-DNA region AH003392.2
                     /ApEinfo_fwdcolor="#ffff66"
                     /ApEinfo_revcolor="#ffff66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1074..1508)
                     /locus_tag="SpecR"
                     /label=SpecR
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    46..48
                     /locus_tag="BsaI recomposition"
                     /label=BsaI recomposition
                     /ApEinfo_fwdcolor="#7ffe07"
                     /ApEinfo_revcolor="#7ffe07"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1506..1508)
                     /locus_tag="BspMI(1)"
                     /label=BspMI(1)
                     /ApEinfo_label="BspMI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    257..259
                     /locus_tag="BsaI site recomposition"
                     /label=BsaI site recomposition
                     /ApEinfo_fwdcolor="#7ffe07"
                     /ApEinfo_revcolor="#7ffe07"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1074..1508)
                     /locus_tag="Spec gene(1)"
                     /label=Spec gene(1)
                     /ApEinfo_label="Spec gene"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="#ffc100"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    257..257
                     /locus_tag="N spacer"
                     /label=N spacer
                     /ApEinfo_fwdcolor="#ffff66"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1510..1865)
                     /locus_tag="SpecR(1)"
                     /label=SpecR(1)
                     /ApEinfo_label="SpecR"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    48..48
                     /locus_tag="N spacer(1)"
                     /label=N spacer(1)
                     /ApEinfo_label="N spacer"
                     /ApEinfo_fwdcolor="#ffff66"
                     /ApEinfo_revcolor="#ffff66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1626..1626
                     /locus_tag="Mut G->A, silent"
                     /label=Mut G->A, silent
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1631..1636)
                     /locus_tag="BpmI"
                     /label=BpmI
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    49..52
                     /label=Overhang
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1900..1900
                     /locus_tag="Mut C->A"
                     /label=Mut C->A
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(253..256)
                     /label=Overhang(1)
                     /ApEinfo_label="Overhang"
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2032..2033)
                     /locus_tag="BspMI(2)"
                     /label=BspMI(2)
                     /ApEinfo_label="BspMI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    53..252
                     /label=uLoop-O-spacer2
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2028..2030)
                     /locus_tag="BspMI(3)"
                     /label=BspMI(3)
                     /ApEinfo_label="BspMI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1510..2046)
                     /locus_tag="pro-CDS-Ter of SpecR"
                     /label=pro-CDS-Ter of SpecR
                     /ApEinfo_fwdcolor="#feffa4"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1074..1508)
                     /locus_tag="pro-CDS-Ter of SpecR(1)"
                     /label=pro-CDS-Ter of SpecR(1)
                     /ApEinfo_label="pro-CDS-Ter of SpecR"
                     /ApEinfo_fwdcolor="#feffa4"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1506..1508)
                     /locus_tag="BspMI(4)"
                     /label=BspMI(4)
                     /ApEinfo_label="BspMI"
                     /ApEinfo_fwdcolor="#ff1827"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1524..1542
                     /locus_tag="SpecR R"
                     /label=SpecR R
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1541..1560)
                     /locus_tag="SpecR F"
                     /label=SpecR F
                     /ApEinfo_fwdcolor="#008080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1510..1511)
                     /locus_tag="BspMI(5)"
                     /label=BspMI(5)
                     /ApEinfo_label="BspMI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    836..836
                     /locus_tag="pMB1 high-copy number mutation"
                     /label=pMB1 high-copy number mutation
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(42..45)
                     /locus_tag="BsaI"
                     /label=BsaI
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    260..263
                     /locus_tag="BsaI(1)"
                     /label=BsaI(1)
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    305..334
                     /locus_tag="Extra region of pBR322"
                     /label=Extra region of pBR322
                     /ApEinfo_fwdcolor="#66ffcc"
                     /ApEinfo_revcolor="#66ffcc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1..40
                     /locus_tag="UNS1"
                     /label=UNS1
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    265..304
                     /locus_tag="UNSX"
                     /label=UNSX
                     /ApEinfo_fwdcolor="#9882ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 CATTACTCGC ATCCATTCTC AGGCTGTCTC GTCTCGTCTC aggtcTCCgg agGCTGGAGG
       61 GAAACATCGT GCGTTAGTGA TTAGGGGAAC CCTTCCTTAC CCGCAAGAAA ATGAAACTGT
      121 AATACCAATA GACCTACACC CTATTTTAAT AGAACACTGC CAGAACCCTT TATGGAGGGC
      181 TTGGACTCGC GGTAAAAGCT TAGAGAAACT CACGACAAAC GAAACTGGAC CTGAACTGAA
      241 GCCCGATCGC CAcgctCGAg accaCCAGGA TACATAGATT ACCACAACTC CGAGCCCTTC
      301 CACCcacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
      361 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      421 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      481 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      541 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      601 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      661 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      721 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      781 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      841 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      901 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      961 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1021 agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtga ttattatttg
     1081 ccgactacct tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg
     1141 cgcgaggcca agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg
     1201 ggctgatact gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg
     1261 attttgccgg ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca
     1321 tcgccagccc agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat
     1381 agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg
     1441 ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg
     1501 aagataccag caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct
     1561 ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga
     1621 atctcactct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc
     1681 gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc
     1741 aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg
     1801 cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc
     1861 ctcatgcgaa acgatcctca tcctgtctct tgatcagata ttgatcccct gcgccatcag
     1921 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag
     1981 ggcgccccag ctggcaattc cggttcgctt gctaagcttg catgcctgca agtcgactct
     2041 agaggagcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
     2101 ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc
     2161 ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact
     2221 gatgggctgc ctgtatcgag tggtgatttt gtgccgagct  
//
