LOCUS       AF_URA3                 3119 bp    ds-DNA  linear   UNK 13-NOV-2018
DEFINITION  .
ACCESSION   AF_URA3
VERSION     AF_URA3
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     
            
            ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        complement(1532..1679)
                     /locus_tag="specR_promoter_from_pKD13"
                     /label="specR_promoter_from_pKD13"
                     /ApEinfo_label="specR_promoter_from_pKD13"
                     /ApEinfo_fwdcolor="#a5ce0b"
                     /ApEinfo_revcolor="#a5ce0b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        1979..2155
                     /locus_tag="ScURA3 Promoter"
                     /label="ScURA3 Promoter"
                     /ApEinfo_label="ScURA3 Promoter"
                     /ApEinfo_fwdcolor="#ffb334"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1975..1978
                     /locus_tag="A - Overhang"
                     /label="A - Overhang"
                     /ApEinfo_label="A - Overhang"
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      2960..3037
                     /locus_tag="ScURA3 Terminator"
                     /label="ScURA3 Terminator"
                     /ApEinfo_label="ScURA3 Terminator"
                     /ApEinfo_fwdcolor="#ffcb72"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(61..649)
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
                     /locus_tag="pMB1-pUC19 ORI variant"
                     /label="pMB1-pUC19 ORI variant"
                     /ApEinfo_label="pMB1-pUC19 ORI variant"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1176..1531)
                     /locus_tag="Spec gene"
                     /label="Spec gene"
                     /ApEinfo_label="Spec gene"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="#ffc100"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1176..1177)
                     /locus_tag="BspMI"
                     /label="BspMI"
                     /ApEinfo_label="BspMI"
                     /ApEinfo_fwdcolor="#ff1827"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1885..1926)
                     /locus_tag="In T-DNA region AH003392.2"
                     /label="In T-DNA region AH003392.2"
                     /ApEinfo_label="In T-DNA region AH003392.2"
                     /ApEinfo_fwdcolor="#ffff66"
                     /ApEinfo_revcolor="#ffff66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(3038..3041)
                     /locus_tag="F - Overhang"
                     /label="F - Overhang"
                     /ApEinfo_label="F - Overhang"
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(740..1174)
                     /locus_tag="SpecR"
                     /label="SpecR"
                     /ApEinfo_label="SpecR"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2156..2959
                     /locus_tag="URA3"
                     /label="URA3"
                     /ApEinfo_label="URA3"
                     /ApEinfo_fwdcolor="#ff9127"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1172..1174)
                     /locus_tag="BspMI(1)"
                     /label="BspMI(1)"
                     /ApEinfo_label="BspMI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(740..1174)
                     /locus_tag="Spec gene(1)"
                     /label="Spec gene(1)"
                     /ApEinfo_label="Spec gene"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="#ffc100"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3090..3119
                     /locus_tag="Extra region of pBR322"
                     /label="Extra region of pBR322"
                     /ApEinfo_label="Extra region of pBR322"
                     /ApEinfo_fwdcolor="#66ffcc"
                     /ApEinfo_revcolor="#66ffcc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1927..1966
                     /locus_tag="UNS1"
                     /label="UNS1"
                     /ApEinfo_label="UNS1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1176..1531)
                     /locus_tag="SpecR(1)"
                     /label="SpecR(1)"
                     /ApEinfo_label="SpecR"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1292
                     /locus_tag="Mut G->A, silent"
                     /label="Mut G->A, silent"
                     /ApEinfo_label="Mut G->A, silent"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1297..1302)
                     /locus_tag="BpmI"
                     /label="BpmI"
                     /ApEinfo_label="BpmI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1566
                     /locus_tag="Mut C->A"
                     /label="Mut C->A"
                     /ApEinfo_label="Mut C->A"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1698..1699)
                     /locus_tag="BspMI(2)"
                     /label="BspMI(2)"
                     /ApEinfo_label="BspMI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1694..1696)
                     /locus_tag="BspMI(3)"
                     /label="BspMI(3)"
                     /ApEinfo_label="BspMI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1176..1712)
                     /locus_tag="pro-CDS-Ter of SpecR"
                     /label="pro-CDS-Ter of SpecR"
                     /ApEinfo_label="pro-CDS-Ter of SpecR"
                     /ApEinfo_fwdcolor="#feffa4"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(740..1174)
                     /locus_tag="pro-CDS-Ter of SpecR(1)"
                     /label="pro-CDS-Ter of SpecR(1)"
                     /ApEinfo_label="pro-CDS-Ter of SpecR"
                     /ApEinfo_fwdcolor="#feffa4"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1172..1174)
                     /locus_tag="BspMI(4)"
                     /label="BspMI(4)"
                     /ApEinfo_label="BspMI"
                     /ApEinfo_fwdcolor="#ff1827"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1190..1208
                     /locus_tag="SpecR R"
                     /label="SpecR R"
                     /ApEinfo_label="SpecR R"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1207..1226)
                     /locus_tag="SpecR F"
                     /label="SpecR F"
                     /ApEinfo_label="SpecR F"
                     /ApEinfo_fwdcolor="#008080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1176..1177)
                     /locus_tag="BspMI(5)"
                     /label="BspMI(5)"
                     /ApEinfo_label="BspMI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    502
                     /locus_tag="pMB1 high-copy number mutation"
                     /label="pMB1 high-copy number mutation"
                     /ApEinfo_label="pMB1 high-copy number mutation"
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     BsaI_F          1968..1973
                     /locus_tag="BsaI_F"
                     /label="BsaI_F"
                     /ApEinfo_label="BsaI_F"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3050..3089
                     /locus_tag="UNSX"
                     /label="UNSX"
                     /ApEinfo_label="UNSX"
                     /ApEinfo_fwdcolor="#9882ff"
                     /ApEinfo_revcolor="#9882ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     BsaI_R          complement(3043..3048)
                     /locus_tag="BsaI_R"
                     /label="BsaI_R"
                     /ApEinfo_label="BsaI_R"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2674
                     /locus_tag="mut SapI"
                     /label="mut SapI"
                     /ApEinfo_label="mut SapI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
       61 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      541 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      721 catgcattct aggtgattat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
      781 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
      841 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
      901 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
      961 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
     1021 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
     1081 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
     1141 gatcaatgtc gatcgtggct ggctcgaaga taccagcaag aatgtcattg cgctgccatt
     1201 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
     1261 caatggtgac ttctacagcg cggagaatct cactctctcc aggggaagcc gaagtttcca
     1321 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
     1381 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
     1441 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
     1501 tcgatacttc ggcgatcacc gcttccctca tgcgaaacga tcctcatcct gtctcttgat
     1561 cagatattga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt
     1621 tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgcta
     1681 agcttgcatg cctgcaagtc gactctagag gagcggtgat cacaggcagc aacgctctgt
     1741 catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct
     1801 tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg
     1861 ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc
     1921 cgagctcatt actcgcatcc attctcaggc tgtctcgtct cgtctcaggt ctcaggagcg
     1981 gtttccttga aatttttttg attcggtaat ctccgaacag aaggaagaac gaaggaagga
     2041 gcacagactt agattggtat atatacgcat atgtagtgtt gaagaaacat gaaattgccc
     2101 agtattctta acccaactgc acagaacaaa aacgtgcagg aaacgaagat aaatcatgtc
     2161 gaaagctaca tataaggaac gtgctgctac tcatcctagt cctgttgctg ccaagctatt
     2221 taatatcatg cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa
     2281 ggaattactg gagttagttg aagcattagg tcccaaaatt tgtttactaa aaacacatgt
     2341 ggatatcttg actgattttt ccatggaggg cacagttaag ccgctaaagg cattatccgc
     2401 caagtacaat tttttactct tcgaggacag aaaatttgct gacattggta atacagtcaa
     2461 attgcagtac tctgcgggtg tatacagaat agcagaatgg gcagacatta cgaatgcaca
     2521 cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa
     2581 ggaacctaga ggccttttga tgttagcaga attgtcatgc aagggctccc tatctactgg
     2641 agaatatact aagggtactg ttgacattgc gaaaagcgac aaagattttg ttatcggctt
     2701 tattgctcaa agagacatgg gtggaagaga tgaaggttac gattggttga ttatgacacc
     2761 cggtgtgggt ttagatgaca agggagatgc attgggtcaa cagtatagaa ccgtggatga
     2821 tgtggtttct acaggatctg acattattat tgttggaaga ggactatttg caaagggaag
     2881 ggatgctaag gtagagggtg aacgttacag aaaagcaggc tgggaagcat atttgagaag
     2941 atgcggccag caaaactaaa aaactgtatt ataagtaaat gcatgtatac taaactcaca
     3001 aattagagct tcaatttaat tatatcagtt attaccccgc ttgagaccac caggatacat
     3061 agattaccac aactccgagc ccttccaccc acagaatcag gggataacgc aggaaagaa
//
