LOCUS       pEPKKα1KN0253           7864 bp ds-DNA     circular     28-OCT-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    320..337
                     /label="pDGB3 alpha FP"
     misc_feature    365..390
                     /label="LB"
     Misc._feature   407..410
                     /label="GAGG 4bp overhang"
     Promoter        411..686
                     /label="nos-p"
     misc_feature    691..757
                     /label="TMV omega"
     CDS             762..1435
                     /label="CUP2"
     misc_feature    1436..1439
                     /label="GCCA"
     CDS             1440..1691
                     /label="Gal4AD"
     MIsc._Feature   1692..1695
                     /label="GCTT 4bp overhang"
     Terminator      1696..1899
                     /label="35S ter"
     misc_feature    1900..1903
                     /label="CGCT"
     misc_feature    1904..1907
                     /label="GTCA"
     misc_feature    1920..1944
                     /label="RB"
     misc_feature    complement(1933..1950)
                     /label="pDGB3 alpha RP"
     misc_feature    complement(3006..4006)
                     /label="STA\region\from\pVS1\plasmid"
     misc_feature    complement(4599..5599)
                     /label="pVS1-Rep\ori"
     misc_feature    complement(6009..6269)
                     /label="pBR322"
     rep_origin      complement(6409..6689)
                     /label="pBR322\ori"
     CDS             complement(6984..7796)
                     /label="KanR"
ORIGIN
        1 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
       61 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
      121 ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
      181 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
      241 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
      301 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
      361 ctggtggcag gatatattgt ggtgtaaaca taacgaattc gtctcaGGAG AGCGGAGAAT
      421 TAAGGGAGTC ACGTTATGAC CCCCGCCGAT GACGCGGGAC AAGCCGTTTT ACGTTTGGAA
      481 CTGACAGAAC CGCAACGTTG AAGGAGCCAC TCAGCCGCGG GTTTCTGGAG TTTAATGAGC
      541 TAAGCACATA CGTCAGAAAC CATTATTGCG CGTTCAAAAG TCGCCTAAGG TCACTATCAG
      601 CTAGCAAATA TTTCTTGTCA AAAATGCTCC ACTGACGTTC CATAAATTCC CCTCGGTATC
      661 CAATTAGAGT CTCATATTCA CTCTCATACT GTATTTTTAC AACAATTACC AACAACAACA
      721 AACAACAAAC AACATTACAA TTACTATTTA CAATTACAAT GATGGTCGTA ATTAACGGGG
      781 TCAAATATGC CTGTGAAACG TGTATCAGGG GTCACAGGGC GGCGCAGTGT ACTCACACTG
      841 ATGGTCCGCT ACAGATGATC AGACGCAAGG GAAGACCATC GACCACATGT GGCCATTGTA
      901 AAGAGCTGAG AAGAACCAAG AACTTCAACC CATCCGGTGG GTGCATGTGT GCCTCTGCAC
      961 GACGGCCAGC TGTTGGCAGC AAGGAAGATG AAACACGATG TCGTTGTGAT GAGGGTGAAC
     1021 CTTGTAAATG TCATACCAAG AGGAAAAGCA GCCGGAAATC AAAGGGAGGG TCATGCCACA
     1081 GAAGGGCAAA TGATGAAGCA GCGCATGTCA ATGGTCTTGG TATTGCAGAT CTGGACGTTC
     1141 TTTTGGGCCT AAATGGTCGC TCGTCGGATG TAGACATGAC AACCACATTG CCGAGTTTGA
     1201 AGCCACCTCT GCAAAACGGA GAAATTAAGG CCGACAGCAT TGACAATCTT GATTTGGCTT
     1261 CCCTCGATCC GCTTGAGCAA AGCCCTAGTA TATCTATGGA ACCTGTTAGT ATCAATGAAA
     1321 CAGGAAGCGC ATATACAACT ACGAACACAG CACTAAACGA TATTGACATT CCATTCTCCA
     1381 TCAATGAGTT GAACGAGCTA TACAAACAAG TATCTTCGCA TAACTCACAT TCACAGCCAa
     1441 tgacaaattc tcaagcgctt tcacaaccaa ttgcctcctc taacgttcat gataacttca
     1501 tgaataatga aatcacggct agtaaaattg atgatggtaa taattcaaaa ccactgtcac
     1561 ctggttggac ggaccaaact gcgtataacg cgtttggaat cactacaggg atgtttaata
     1621 ccactacaat ggatgatgta tataactatc tattcgatga tgaagatacc ccaccaaacc
     1681 caaaaaaaga gGCTTCTCTA GCTAGAGTCG ATCGACAAGC TCGAGTTTCT CCATAATAAT
     1741 GTGTGAGTAG TTCCCAGATA AGGGAATTAG GGTTCCTATA GGGTTTCGCT CATGTGTTGA
     1801 GCATATAAGA AACCCTTAGT ATGTATTTGT ATTTGTAAAA TACTTCTATC AATAAAATTT
     1861 CTAATTCCTA AAACCAAAAT CCAGTACTAA AATCCAGATc gctgtcatga gacgaattct
     1921 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
     1981 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag
     2041 ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc
     2101 ttctgacgtt cagtgcagcc gtcatctgaa aacgacatgt cgcacaagtc ctaagttacg
     2161 cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata
     2221 aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct
     2281 ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc
     2341 gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc
     2401 gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg
     2461 accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc
     2521 caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg
     2581 gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc
     2641 atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc
     2701 cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc
     2761 cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca
     2821 cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag
     2881 gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa
     2941 gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg
     3001 cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacctctca accgtgcggc
     3061 tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg
     3121 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct
     3181 tgcgtcatgc ggtcgctgcg tatatgatcc gatgagtaaa taaacaaata cgcaagggga
     3241 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgg
     3301 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga
     3361 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt
     3421 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt
     3481 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga
     3541 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt
     3601 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt
     3661 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta
     3721 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc
     3781 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc
     3841 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc
     3901 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg
     3961 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag
     4021 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta
     4081 tctgaataga tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat
     4141 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga
     4201 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgtctgccg
     4261 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgacggt cgcaaaccat
     4321 ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg
     4381 cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag
     4441 cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga
     4501 ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg
     4561 tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg
     4621 accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg
     4681 cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc
     4741 atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt
     4801 tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga
     4861 aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta
     4921 cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc
     4981 gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg
     5041 attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg
     5101 attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg
     5161 caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg
     5221 gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg
     5281 agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca
     5341 acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa
     5401 ttgccctagc aggggaaaaa ggtcgaaaag gactctttcc tgtggatagc acgtacattg
     5461 ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca
     5521 ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg
     5581 cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt
     5641 ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc
     5701 tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc
     5761 tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc
     5821 gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     5881 atgcagctcc cggtgacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     5941 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     6001 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     6061 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     6121 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6181 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6241 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6301 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6361 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6421 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6481 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6541 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6601 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6661 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6721 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     6781 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6841 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6901 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6961 tggtcatgca ttctaggtga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
     7021 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
     7081 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
     7141 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
     7201 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc
     7261 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     7321 ccgttattca ttcgtgattg cgcctgagcg agtcgaaata cgcgatcgct gttaaaagga
     7381 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
     7441 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc tgggatcgca
     7501 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     7561 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
     7621 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcggtagatt
     7681 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
     7741 atgttggaat ttaatcgcgg ccttgagcaa gacgtttccc gttgaatatg gctcataaca
     7801 gaacttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
     7861 taac
//