LOCUS       pEPCTΩSP0018           10500 bp ds-DNA     circular     09-MAR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             7..90
                     /label="C terminal 3xFLAG (FLAG octapeptide) tag"
     Terminator      95..298
                     /label="35S ter"
     misc_feature    303..306
                     /label="GTCA"
     misc_feature    320..344
                     /label="RB"
     misc_feature    complement(1406..2406)
                     /label="STA\region\from\pVS1\plasmid"
     misc_feature    2999..3999
                     /label="pVS1-Rep\ori"
     misc_feature    complement(4409..4669)
                     /label="pBR322"
     rep_origin      complement(4809..5089)
                     /label="pBR322\ori"
     CDS             complement(5381..6391)
                     /label="SpmR"
     promoter        complement(6392..6525)
                     /label="Spm\Promoter"
     misc_feature    6958..6983
                     /label="LB"
     Promoter        7005..7430
                     /label="CaMV 35S promoter short"
     5'_UTR          7447..7508
                     /label="TMV Omega"
     misc_feature    7521..8029
                     /label="NanoLuc"
     CDS             8034..8117
                     /label="C terminal 3xFLAG (FLAG octapeptide) tag"
     Terminator      8122..8325
                     /label="35S ter"
     Promoter        8338..8763
                     /label="CaMV 35S promoter short"
     5'_UTR          8780..8841
                     /label="TMV Omega"
ORIGIN
        1 ggTTCGGATT ATAAGGACCA TGACGGAGAC TATAAGGACC ATGACCTCGA CGCTGCAGCA
       61 GCGGATTATA AGGACGATGA CGATAAGTGA GCTTCTCTAG CTAGAGTCGA TCGACAAGCT
      121 CGAGTTTCTC CATAATAATG TGTGAGTAGT TCCCAGATAA GGGAATTAGG GTTCCTATAG
      181 GGTTTCGCTC ATGTGTTGAG CATATAAGAA ACCCTTAGTA TGTATTTGTA TTTGTAAAAT
      241 ACTTCTATCA ATAAAATTTC TAATTCCTAA AACCAAAATC CAGTACTAAA ATCCAGATCG
      301 CTgtcatgag accggatcct gacaggatat attggcgggt aaacctaaga gaaaagagcg
      361 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
      421 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
      481 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcatctgaa aacgacatgt
      541 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
      601 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
      661 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
      721 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
      781 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
      841 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
      901 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
      961 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
     1021 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
     1081 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
     1141 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
     1201 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
     1261 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
     1321 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
     1381 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
     1441 gcacctctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
     1501 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
     1561 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatcc gatgagtaaa
     1621 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
     1681 tcaggcaaga cgaccatcgg aacccatcta gcccgcgccc tgcaactcgc cggggccgat
     1741 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
     1801 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
     1861 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
     1921 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
     1981 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
     2041 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
     2101 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
     2161 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
     2221 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
     2281 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
     2341 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
     2401 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
     2461 agaccattac cgagctgcta tctgaataga tcgcgcagct accagagtaa atgagcaaat
     2521 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
     2581 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
     2641 agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
     2701 gcgtgacggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct
     2761 ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg
     2821 ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc
     2881 ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt
     2941 cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt
     3001 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga
     3061 cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct
     3121 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg
     3181 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg
     3241 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac
     3301 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga
     3361 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta
     3421 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga
     3481 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta
     3541 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta
     3601 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat
     3661 cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat
     3721 cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac
     3781 ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gactctttcc
     3841 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
     3901 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
     3961 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
     4021 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
     4081 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
     4141 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc
     4201 gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg
     4261 acggtgaaaa cctctgacac atgcagctcc cggtgacggt cacagcttgt ctgtaagcgg
     4321 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     4381 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     4441 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     4501 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     4561 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     4621 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4681 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4741 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4801 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4861 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4921 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4981 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5041 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5101 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     5161 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5221 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5281 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5341 aaactcacgt taagggattt tggtcatgca ttctaggtga ttatttgccg actaccttgg
     5401 tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc
     5461 gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg
     5521 ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta
     5581 ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatca ccagcccagt
     5641 cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag
     5701 gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg
     5761 cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa
     5821 gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg
     5881 gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc
     5941 caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa
     6001 gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca
     6061 ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc
     6121 caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc ataatgttta
     6181 actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac
     6241 atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtaccccaa
     6301 aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg
     6361 tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagc ttacgaaccg
     6421 aacaggctta tgtccactgg gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg
     6481 caaccttggg tagcagcgaa gtcgaggcat ttctgtcctg gctggaacag aacttattat
     6541 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
     6601 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
     6661 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt
     6721 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca
     6781 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag
     6841 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
     6901 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg
     6961 caggatatat tgtggtgtaa acataacgga tccggtctca ggagGTCAAC ATGGTGGAGC
     7021 ACGACACTCT GGTCTACTCC AAAAATGTCA AAGATACAGT CTCAGAAGAT CAAAGGGCTA
     7081 TTGAGACTTT TCAACAAAGG ATAATTTCGG GAAACCTCCT CGGATTCCAT TGCCCAGCTA
     7141 TCTGTCACTT CATCGAAAGG ACAGTAGAAA AGGAAGGTGG CTCCTACAAA TGCCATCATT
     7201 GCGATAAAGG AAAGGCTATC ATTCAAGATC TCTCTGCCGA CAGTGGTCCC AAAGATGGAC
     7261 CCCCACCCAC GAGGAGCATC GTGGAAAAAG AAGAGGTTCC AACCACGTCT ACAAAGCAAG
     7321 TGGATTGATG TGACATCTCC ACTGACGTAA GGGATGACGC ACAATCCCAC TATCCTTCGC
     7381 AAGACCCTTC CTCTATATAA GGAAGTTCAT TTCATTTGGA GAGGACACGC TCGAGTATAA
     7441 GAGCTCATTT TTACAACAAT TACCAACAAC AACAAACAAC AAACAACATT ACAATTACAT
     7501 TTACAATTAT CGATACAATG GTATTCACAC TCGAAGATTT CGTTGGGGAC TGGCGACAGA
     7561 CAGCCGGCTA CAACCTGGAC CAAGTCCTTG AACAGGGAGG TGTGTCCAGT TTGTTTCAGA
     7621 ATCTCGGGGT GTCCGTAACT CCGATCCAAA GGATTGTCCT GAGCGGTGAA AATGGGCTGA
     7681 AGATCGACAT CCATGTCATC ATCCCGTATG AAGGTCTGAG CGGCGACCAA ATGGGCCAGA
     7741 TCGAAAAAAT TTTTAAGGTG GTGTACCCTG TGGATGATCA TCACTTTAAG GTGATCCTGC
     7801 ACTATGGCAC ACTGGTAATC GACGGGGTTA CGCCGAACAT GATCGACTAT TTCGGACGGC
     7861 CGTATGAAGG CATCGCCGTG TTCGACGGCA AAAAGATCAC TGTAACAGGG ACCCTGTGGA
     7921 ACGGCAACAA AATTATCGAC GAGCGCCTGA TCAACCCCGA CGGCTCCCTG CTGTTCCGAG
     7981 TAACCATCAA CGGAGTGACC GGCTGGCGGC TGTGCGAACG CATTCTGGCT TCGGATTATA
     8041 AGGACCATGA CGGAGACTAT AAGGACCATG ACCTCGACGC TGCAGCAGCG GATTATAAGG
     8101 ACGATGACGA TAAGTGAGCT TCTCTAGCTA GAGTCGATCG ACAAGCTCGA GTTTCTCCAT
     8161 AATAATGTGT GAGTAGTTCC CAGATAAGGG AATTAGGGTT CCTATAGGGT TTCGCTCATG
     8221 TGTTGAGCAT ATAAGAAACC CTTAGTATGT ATTTGTATTT GTAAAATACT TCTATCAATA
     8281 AAATTTCTAA TTCCTAAAAC CAAAATCCAG TACTAAAATC CAGATCGCTg tcaggagGTC
     8341 AACATGGTGG AGCACGACAC TCTGGTCTAC TCCAAAAATG TCAAAGATAC AGTCTCAGAA
     8401 GATCAAAGGG CTATTGAGAC TTTTCAACAA AGGATAATTT CGGGAAACCT CCTCGGATTC
     8461 CATTGCCCAG CTATCTGTCA CTTCATCGAA AGGACAGTAG AAAAGGAAGG TGGCTCCTAC
     8521 AAATGCCATC ATTGCGATAA AGGAAAGGCT ATCATTCAAG ATCTCTCTGC CGACAGTGGT
     8581 CCCAAAGATG GACCCCCACC CACGAGGAGC ATCGTGGAAA AAGAAGAGGT TCCAACCACG
     8641 TCTACAAAGC AAGTGGATTG ATGTGACATC TCCACTGACG TAAGGGATGA CGCACAATCC
     8701 CACTATCCTT CGCAAGACCC TTCCTCTATA TAAGGAAGTT CATTTCATTT GGAGAGGACA
     8761 CGCTCGAGTA TAAGAGCTCA TTTTTACAAC AATTACCAAC AACAACAAAC AACAAACAAC
     8821 ATTACAATTA CATTTACAAT TATCGATACA ATGgaGgacg ccaaaaacat aaagaaaggc
     8881 ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg
     8941 aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac
     9001 atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat
     9061 gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg
     9121 ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat
     9181 gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa
     9241 aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt
     9301 atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct
     9361 catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga tagggacaag
     9421 acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg tgtcgctctg
     9481 cctcatagaa ctgcctgcgt gagattctcg catgccagag atcctatttt tggcaatcaa
     9541 atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt
     9601 actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa
     9661 gaActgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct ggtgccaacc
     9721 ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac
     9781 gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt tgccaagagg
     9841 ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc agctattctg
     9901 attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa
     9961 gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag aggcgaactg
    10021 tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc
    10081 ttgattgaca aggatggatg gctacattct ggagacatag cttactggga cgaGgacgaa
    10141 cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta tcaggtggct
    10201 cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc aggtgtcgca
    10261 ggtctGcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga
    10321 aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa
    10381 aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc
    10441 gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa gatcgccgtg
//