LOCUS       pEPCT⍺KN0001            8836 bp ds-DNA     circular     26-APR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    6..9
                     /label="GTCA"
     misc_feature    22..46
                     /label="RB"
     misc_feature    complement(35..52)
                     /label="pDGB3 alpha RP"
     misc_feature    complement(1108..2108)
                     /label="STA\region\from\pVS1\plasmid"
     misc_feature    complement(2701..3701)
                     /label="pVS1-Rep\ori"
     misc_feature    complement(4111..4371)
                     /label="pBR322"
     rep_origin      complement(4511..4791)
                     /label="pBR322\ori"
     CDS             complement(5086..5898)
                     /label="KanR"
     misc_feature    6286..6303
                     /label="pDGB3 alpha FP"
     misc_feature    6331..6356
                     /label="LB"
     Promoter        6377..6802
                     /label="CaMV 35S promoter short"
     5'_UTR          6819..6880
                     /label="TMV Omega"
     CDS             8546..8629
                     /label="C terminal 3xFLAG (FLAG octapeptide) tag"
     Terminator      8634..8836
                     /label="35S ter"
ORIGIN
        1 TCGCTgtcat gagacgaatt ctgacaggat atattggcgg gtaaacctaa gagaaaagag
       61 cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt
      121 tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct
      181 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcatctg aaaacgacat
      241 gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct
      301 tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc
      361 catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca
      421 ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga
      481 gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg
      541 ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct
      601 actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc
      661 gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc
      721 cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc
      781 ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg
      841 cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca
      901 tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag
      961 gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga
     1021 gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt
     1081 ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc
     1141 gcgcacctct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg
     1201 gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga
     1261 tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat ccgatgagta
     1321 aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg
     1381 ggtcaggcaa gacgaccatc ggaacccatc tagcccgcgc cctgcaactc gccggggccg
     1441 atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg
     1501 aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg
     1561 ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg
     1621 tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca
     1681 tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa
     1741 ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg
     1801 ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg
     1861 tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg
     1921 acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta
     1981 atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc
     2041 acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt
     2101 caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg
     2161 caagaccatt accgagctgc tatctgaata gatcgcgcag ctaccagagt aaatgagcaa
     2221 atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac
     2281 aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg
     2341 taagcggctg ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat
     2401 cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac
     2461 ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca
     2521 cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg
     2581 ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt
     2641 ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc
     2701 gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca
     2761 gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac
     2821 ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag
     2881 ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg
     2941 cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc
     3001 acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc
     3061 gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag
     3121 tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg
     3181 gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc
     3241 taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc
     3301 tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg
     3361 atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg
     3421 atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt
     3481 acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggactcttt
     3541 cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac
     3601 attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa
     3661 gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc
     3721 cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct
     3781 acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct
     3841 ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg
     3901 ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga
     3961 tgacggtgaa aacctctgac acatgcagct cccggtgacg gtcacagctt gtctgtaagc
     4021 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     4081 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
     4141 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     4201 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     4261 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4321 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4381 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4441 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4501 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4561 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4621 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4681 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4741 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4801 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     4861 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4921 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4981 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5041 gaaaactcac gttaagggat tttggtcatg cattctaggt gattagaaaa actcatcgag
     5101 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
     5161 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
     5221 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
     5281 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
     5341 caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc
     5401 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagtcgaaa
     5461 tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa
     5521 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
     5581 tgctgttttc cctgggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
     5641 atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc
     5701 tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
     5761 cttcccatac aatcggtaga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
     5821 atacccatat aaatcagcat ccatgttgga atttaatcgc ggccttgagc aagacgtttc
     5881 ccgttgaata tggctcataa cagaacttat tatttccttc ctcttttcta cagtatttaa
     5941 agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct
     6001 aaaaccttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca
     6061 tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc
     6121 gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt
     6181 tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct
     6241 cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag
     6301 ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacataac
     6361 gaattcgtct caggagGTCA ACATGGTGGA GCACGACACT CTGGTCTACT CCAAAAATGT
     6421 CAAAGATACA GTCTCAGAAG ATCAAAGGGC TATTGAGACT TTTCAACAAA GGATAATTTC
     6481 GGGAAACCTC CTCGGATTCC ATTGCCCAGC TATCTGTCAC TTCATCGAAA GGACAGTAGA
     6541 AAAGGAAGGT GGCTCCTACA AATGCCATCA TTGCGATAAA GGAAAGGCTA TCATTCAAGA
     6601 TCTCTCTGCC GACAGTGGTC CCAAAGATGG ACCCCCACCC ACGAGGAGCA TCGTGGAAAA
     6661 AGAAGAGGTT CCAACCACGT CTACAAAGCA AGTGGATTGA TGTGACATCT CCACTGACGT
     6721 AAGGGATGAC GCACAATCCC ACTATCCTTC GCAAGACCCT TCCTCTATAT AAGGAAGTTC
     6781 ATTTCATTTG GAGAGGACAC GCTCGAGTAT AAGAGCTCAT TTTTACAACA ATTACCAACA
     6841 ACAACAAACA ACAAACAACA TTACAATTAC ATTTACAATT ATCGATACAA TGgaGgacgc
     6901 caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga
     6961 gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga
     7021 tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc
     7081 agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa
     7141 ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc
     7201 cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac
     7261 cgtggtgttc gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc
     7321 aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat
     7381 gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga
     7441 gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct
     7501 gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga
     7561 tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca
     7621 tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt
     7681 aatgtataga tttgaagaag aActgtttct gaggagcctt caggattaca agattcaaag
     7741 tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata
     7801 cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg
     7861 ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga
     7921 gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa
     7981 agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt
     8041 taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa
     8101 tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc
     8161 ttactgggac gaGgacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta
     8221 caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat
     8281 cttcgacgca ggtgtcgcag gtctGcccga cgatgacgcc ggtgaacttc ccgccgccgt
     8341 tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag
     8401 tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa
     8461 aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa
     8521 gggcggaaag atcgccgtgg gTTCGGATTA TAAGGACCAT GACGGAGACT ATAAGGACCA
     8581 TGACCTCGAC GCTGCAGCAG CGGATTATAA GGACGATGAC GATAAGTGAG CTTCTCTAGC
     8641 TAGAGTCGAT CGACAAGCTC GAGTTTCTCC ATAATAATGT GTGAGTAGTT CCCAGATAAG
     8701 GGAATTAGGG TTCCTATAGG GTTTCGCTCA TGTGTTGAGC ATATAAGAAA CCCTTAGTAT
     8761 GTATTTGTAT TTGTAAAATA CTTCTATCAA TAAAATTTCT AATTCCTAAA ACCAAAATCC
     8821 AGTACTAAAA TCCAGA
//