LOCUS       pEPCT⍺KN0005            7696 bp ds-DNA     circular     16-FEB-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(964..1224)
                     /label="pBR322"
     rep_origin      complement(1364..1644)
                     /label="pBR322\ori"
     CDS             complement(1939..2751)
                     /label="KanR"
     misc_feature    3139..3156
                     /label="pDGB3 alpha FP"
     misc_feature    3184..3209
                     /label="LB"
     Promoter        3230..3655
                     /label="CaMV 35S promoter short"
     5'_UTR          3672..3733
                     /label="TMV Omega"
     misc_feature    3746..4254
                     /label="NanoLuc"
     CDS             4259..4342
                     /label="C terminal 3xFLAG (FLAG octapeptide) tag"
     Terminator      4347..4550
                     /label="35S ter"
     misc_feature    4555..4558
                     /label="GTCA"
     misc_feature    4571..4595
                     /label="RB"
     misc_feature    complement(4584..4601)
                     /label="pDGB3 alpha RP"
     misc_feature    complement(5657..6657)
                     /label="STA\region\from\pVS1\plasmid"
     misc_feature    complement(join(7250..7696,1..554))
                     /label="pVS1-Rep\ori"
ORIGIN
        1 atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac
       61 tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc
      121 aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag
      181 ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac
      241 gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg
      301 atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc
      361 ctagcagggg aaaaaggtcg aaaaggactc tttcctgtgg atagcacgta cattgggaac
      421 ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg
      481 aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa
      541 aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc
      601 cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc
      661 cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg
      721 ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca
      781 catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
      841 gctcccggtg acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
      901 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
      961 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     1021 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     1081 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     1141 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1201 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1261 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1321 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1381 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1441 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1501 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1561 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1621 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1681 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1741 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1801 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1861 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1921 atgcattcta ggtgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
     1981 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
     2041 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
     2101 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
     2161 accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac
     2221 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
     2281 attcattcgt gattgcgcct gagcgagtcg aaatacgcga tcgctgttaa aaggacaatt
     2341 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc
     2401 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttccctggga tcgcagtggt
     2461 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa
     2521 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt
     2581 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcggt agattgtcgc
     2641 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
     2701 ggaatttaat cgcggccttg agcaagacgt ttcccgttga atatggctca taacagaact
     2761 tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca
     2821 agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt
     2881 tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac
     2941 cgcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg
     3001 agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa
     3061 catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg
     3121 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
     3181 tggtggcagg atatattgtg gtgtaaacat aacgaattcg tctcaggagG TCAACATGGT
     3241 GGAGCACGAC ACTCTGGTCT ACTCCAAAAA TGTCAAAGAT ACAGTCTCAG AAGATCAAAG
     3301 GGCTATTGAG ACTTTTCAAC AAAGGATAAT TTCGGGAAAC CTCCTCGGAT TCCATTGCCC
     3361 AGCTATCTGT CACTTCATCG AAAGGACAGT AGAAAAGGAA GGTGGCTCCT ACAAATGCCA
     3421 TCATTGCGAT AAAGGAAAGG CTATCATTCA AGATCTCTCT GCCGACAGTG GTCCCAAAGA
     3481 TGGACCCCCA CCCACGAGGA GCATCGTGGA AAAAGAAGAG GTTCCAACCA CGTCTACAAA
     3541 GCAAGTGGAT TGATGTGACA TCTCCACTGA CGTAAGGGAT GACGCACAAT CCCACTATCC
     3601 TTCGCAAGAC CCTTCCTCTA TATAAGGAAG TTCATTTCAT TTGGAGAGGA CACGCTCGAG
     3661 TATAAGAGCT CATTTTTACA ACAATTACCA ACAACAACAA ACAACAAACA ACATTACAAT
     3721 TACATTTACA ATTATCGATA CAATGGTATT CACACTCGAA GATTTCGTTG GGGACTGGCG
     3781 ACAGACAGCC GGCTACAACC TGGACCAAGT CCTTGAACAG GGAGGTGTGT CCAGTTTGTT
     3841 TCAGAATCTC GGGGTGTCCG TAACTCCGAT CCAAAGGATT GTCCTGAGCG GTGAAAATGG
     3901 GCTGAAGATC GACATCCATG TCATCATCCC GTATGAAGGT CTGAGCGGCG ACCAAATGGG
     3961 CCAGATCGAA AAAATTTTTA AGGTGGTGTA CCCTGTGGAT GATCATCACT TTAAGGTGAT
     4021 CCTGCACTAT GGCACACTGG TAATCGACGG GGTTACGCCG AACATGATCG ACTATTTCGG
     4081 ACGGCCGTAT GAAGGCATCG CCGTGTTCGA CGGCAAAAAG ATCACTGTAA CAGGGACCCT
     4141 GTGGAACGGC AACAAAATTA TCGACGAGCG CCTGATCAAC CCCGACGGCT CCCTGCTGTT
     4201 CCGAGTAACC ATCAACGGAG TGACCGGCTG GCGGCTGTGC GAACGCATTC TGGCTTCGGA
     4261 TTATAAGGAC CATGACGGAG ACTATAAGGA CCATGACCTC GACGCTGCAG CAGCGGATTA
     4321 TAAGGACGAT GACGATAAGT GAGCTTCTCT AGCTAGAGTC GATCGACAAG CTCGAGTTTC
     4381 TCCATAATAA TGTGTGAGTA GTTCCCAGAT AAGGGAATTA GGGTTCCTAT AGGGTTTCGC
     4441 TCATGTGTTG AGCATATAAG AAACCCTTAG TATGTATTTG TATTTGTAAA ATACTTCTAT
     4501 CAATAAAATT TCTAATTCCT AAAACCAAAA TCCAGTACTA AAATCCAGAT CGCTgtcatg
     4561 agacgaattc tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
     4621 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat
     4681 gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata
     4741 gtgcagtcgg cttctgacgt tcagtgcagc cgtcatctga aaacgacatg tcgcacaagt
     4801 cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt
     4861 ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga
     4921 gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca
     4981 accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg
     5041 gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg
     5101 ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg
     5161 ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca
     5221 ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc
     5281 gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga
     5341 agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg
     5401 accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc
     5461 tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg
     5521 ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc
     5581 aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga
     5641 agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacctctc
     5701 aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc
     5761 ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga
     5821 gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatc cgatgagtaa ataaacaaat
     5881 acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag
     5941 acgaccatcg gaacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta
     6001 gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg
     6061 ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg
     6121 cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc
     6181 aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc
     6241 gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg
     6301 gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg
     6361 ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca
     6421 ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc
     6481 gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag
     6541 agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca
     6601 aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt
     6661 tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta
     6721 ccgagctgct atctgaatag atcgcgcagc taccagagta aatgagcaaa tgaataaatg
     6781 agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc
     6841 gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg
     6901 gttgtctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgacgg
     6961 tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa
     7021 gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga
     7081 atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg
     7141 tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat
     7201 gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct
     7261 gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt
     7321 agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat
     7381 ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc
     7441 cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg
     7501 cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc
     7561 catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc
     7621 cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat
     7681 cgagctagct gattgg
//