LOCUS       GB_UA_114A_TU_for_const 9756 bp ds-DNA     linear       14-JUN-2019
DEFINITION  .
ACCESSION   GB_UA_114A                                                         
VERSION     GB_UA_114A                                                         
FEATURES             Location/Qualifiers
     promoter        47..1074
                     /label="35s"
     CDS             1077..1328
                     /label="Gal4AD"
     CDS             1077..3233
                     /label="Translation 1077-3233"
     misc_feature    1359..3197
                     /label="PhiC31"
     terminator      complement(3202..3427)
                     /label="T35S"
     misc_feature    3428..3431
                     /label="CGCT"
     misc_feature    3432..3435
                     /label="GTCA"
     misc_feature    3448..3472
                     /label="RB"
     misc_feature    3461..3478
                     /label="alpha RP"
     misc_feature    complement(4534..5534)
                     /label="STA\region\from\pVS1\plasmid"
     misc_feature    complement(6127..7127)
                     /label="pVS1-Rep\ori"
     misc_feature    complement(7537..7797)
                     /label="pBR322"
     rep_origin      complement(7937..8217)
                     /label="pBR322\ori"
     CDS             complement(8512..9324)
                     /label="KanR"
     misc_feature    9712..9729
                     /label="alphaFP"
ORIGIN
        1 tggcaggata tattgtggtg taaacataac gaattcgtct caggagacta gagccaagct
       61 gatctccttt gccccggaga tcaccatgga cgactttctc tatctctacg atctaggaag
      121 aaagttcgac ggagaaggtg acgataccat gttcaccacc gataatgaga agattagcct
      181 cttcaatttc agaaagaatg ctgacccaca gatggttaga gaggcctacg cggcaggtct
      241 gatcaagacg atctacccga gtaataatct ccaggagatc aaataccttc ccaagaaggt
      301 taaagatgca gtcaaaagat tcaggactaa ctgcatcaag aacacagaga aagatatatt
      361 tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc ataaaccaag
      421 gcaagtaata gagattggag tctctaagaa agtagttcct actgaatcaa aggccatgga
      481 gtcaaaaatt cagatcgagg atctaacaga actcgccgtg aagactggcg aacagttcat
      541 acagagtctt ttacgactca atgacaagaa gaaaatcttc gtcaacatgg tggagcacga
      601 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
      661 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
      721 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
      781 taaaggaaag gctatcgttc aagatgcccc tgccgacagt ggtcccaaag atggaccccc
      841 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
      901 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
      961 cccttcctct atataaggaa gttcatttca tttggagagg actccggtat ttttacaaca
     1021 attaccacaa caaaacaaac aacaaacaac attacaattt actattctag tcgaccatga
     1081 caaattctca agcgctttca caaccaattg cctcctctaa cgttcatgat aacttcatga
     1141 ataatgaaat cacggctagt aaaattgatg atggtaataa ttcaaaacca ctgtcacctg
     1201 gttggacgga ccaaactgcg tataacgcgt ttggaatcac tacagggatg tttaatacca
     1261 ctacaatgga tgatgtatat aactatctat tcgatgatga agatacccca ccaaaaccaa
     1321 aaaaagaggg tggcagtgga gggggcggaa gcgggggaat ggacacgtat gccggcgctt
     1381 atgatagaca atcaagagaa agagagaact cttctgccgc ctctccagct acacagagat
     1441 ctgccaacga ggataaagct gctgatttac aaagagaggt agagagagat gggggtaggt
     1501 ttagatttgt gggtcatttc tctgaggctc ctggtacatc agcctttggt actgctgaac
     1561 ggcctgaatt tgaaagaata ttaaatgagt gtcgggccgg tcgactaaat atgataatag
     1621 tttatgatgt atctaggttc agtagactca aagttatgga tgcaattccg atcgtgagcg
     1681 agctacttgc actcggtgtt actatcgtaa gtacacaaga aggtgtattc aggcagggta
     1741 atgtgatgga ccttatacac cttattatgc gactcgacgc tagccataaa gagtcatcac
     1801 tgaagagtgc taaaatcttg gacaccaaga acctgcaaag ggagcttgga ggctatgtag
     1861 gaggaaaagc tccgtatggt ttcgaactcg tgagtgagac aaaagagatt accagaaatg
     1921 gtaggatggt gaacgtggtt attaataagc tagctcatag cacaacccct ctcacagggc
     1981 cttttgagtt cgaacctgac gttattagat ggtggtggag agagatcaaa actcacaaac
     2041 atcttccatt taagccaggt agccaggctg ctatccaccc tggatctatc actgggcttt
     2101 gtaaaaggat ggatgctgac gcagtgccaa ctcgagggga aacaatcggt aagaagactg
     2161 catcttccgc ttgggatccc gctacagtta tgagaatcct tcgggatccg cgtattgctg
     2221 gattcgccgc agaagtcatt tataagaaaa aacctgatgg tactcctact acaaaaatcg
     2281 aaggttatag gatccaaagg gatcctatta ctttgcgtcc agttgaatta gactgtggac
     2341 cgatcatcga acctgccgag tggtacgagt tgcaggcctg gcttgatggt agagggcgtg
     2401 gaaagggttt atctcgggga caggcaattt tgtcagcaat ggataagctt tattgtgagt
     2461 gtggggctgt tatgacatca aaacgtggag aagagtcaat caaggattct tacaggtgca
     2521 gacgtcgtaa agtggtggac ccttctgctc caggacagca tgagggtaca tgcaacgttt
     2581 ctatggctgc tcttgataaa tttgtggccg aacgtatttt caacaagatt agacacgctg
     2641 aaggggatga ggaaacttta gctcttcttt gggaggctgc ccggcgtttt ggtaagctaa
     2701 cagaggcacc agaaaaaagt ggcgaaagag ctaatttggt agccgaaaga gcagacgcct
     2761 tgaatgcatt ggaagaactt tacgaagata gggctgccgg agcatatgat ggaccagttg
     2821 gtcggaagca tttcaggaag caacaagctg cactaacttt gagacagcaa ggcgcagagg
     2881 agcgactcgc agagcttgag gccgccgagg ccccaaagct tccactcgat caatggtttc
     2941 ctgaggatgc cgacgctgat cctacaggtc caaagagctg gtggggaaga gctagcgtag
     3001 acgataagag agtgtttgtg ggtctatttg ttgacaagat tgtagttact aagtccacta
     3061 cgggtcgagg acagggcact cccatcgaaa agagagccag tattacctgg gcaaagcctc
     3121 caaccgatga tgatgaagac gatgcacaag atggtaccga ggatgtggca gcaccaaaaa
     3181 aaaagaggaa ggtgtaagct tcggccatgc tagagtccgc aaaaatcacc agtctctctc
     3241 tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg
     3301 gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     3361 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     3421 gtgacctcgc tgtcatgaga cgaattctga caggatatat tggcgggtaa acctaagaga
     3481 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg
     3541 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca
     3601 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt catctgaaaa
     3661 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg
     3721 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca
     3781 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga
     3841 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt
     3901 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca
     3961 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg
     4021 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc
     4081 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg
     4141 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc
     4201 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca
     4261 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg
     4321 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga
     4381 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc
     4441 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa
     4501 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag
     4561 ccgcccgcgc acctctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc
     4621 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa
     4681 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatccga
     4741 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga
     4801 aaggcgggtc aggcaagacg accatcggaa cccatctagc ccgcgccctg caactcgccg
     4861 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg
     4921 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg
     4981 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact
     5041 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt
     5101 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg
     5161 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg
     5221 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc
     5281 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg
     5341 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt
     5401 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc
     5461 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa
     5521 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg
     5581 ccaaggcaag accattaccg agctgctatc tgaatagatc gcgcagctac cagagtaaat
     5641 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc
     5701 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc
     5761 caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg
     5821 aggaatcggc gtgacggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt
     5881 gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca
     5941 gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg
     6001 caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca
     6061 gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac
     6121 gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag
     6181 cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat
     6241 tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa
     6301 gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc
     6361 tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta
     6421 aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg
     6481 gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg
     6541 ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag
     6601 aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt
     6661 tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag
     6721 acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc
     6781 aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct
     6841 ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc
     6901 taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaagga
     6961 ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac
     7021 ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat
     7081 ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt
     7141 aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa
     7201 gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg
     7261 gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa
     7321 gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt
     7381 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gtgacggtca cagcttgtct
     7441 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     7501 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat
     7561 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     7621 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg
     7681 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7741 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7801 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7861 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7921 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7981 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8041 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8101 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8161 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8221 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     8281 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8341 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8401 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8461 tggaacgaaa actcacgtta agggattttg gtcatgcatt ctaggtgatt agaaaaactc
     8521 atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg
     8581 aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag
     8641 atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc
     8701 ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga
     8761 gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc
     8821 gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag
     8881 tcgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg
     8941 caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac
     9001 ctggaatgct gttttccctg ggatcgcagt ggtgagtaac catgcatcat caggagtacg
     9061 gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat
     9121 ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc
     9181 atcgggcttc ccatacaatc ggtagattgt cgcacctgat tgcccgacat tatcgcgagc
     9241 ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc ttgagcaaga
     9301 cgtttcccgt tgaatatggc tcataacaga acttattatt tccttcctct tttctacagt
     9361 atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg
     9421 cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt
     9481 ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct
     9541 gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag
     9601 cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac
     9661 ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt
     9721 gccgagctgc cggtcgggga gctgttggct ggctgg
//